ISFac1

  • Family ISH3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AAAA01000062 ND Ferroplasma acidarmanus
Ferroplasma acidarmanus Faci_62
DNA section
IS Length : 1477 bp

Ends


IR Length : 18

IRL : CAGACTCTCCGCATGTCTTACCATATAATTTAATTTCTTTGTAAATCCTT
IRR : CAGACTCTCCGCATGTCTATGATAGTGATGCAGAATATCATTAATTTATG

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

CAGACTCTCCGCATGTCTTACCATATAATTTAATTTCTTTGTAAATCCTTGTAGTTTTATGTGTTACAGGGCAAATTATATGTACGACAAATTTAAAATT
GGAGGGAATAATTTTGAATTATGAACAACAAAACAATCCCTCCAAAATTAAATGCCAATAAGGTTAAAAAATTTTACTCTTCTCAGATGTACAATATTTT
CTCAAATAATATACATGTACAGTCAAGGTATAATTCAGGGGATATTGTAAATACAGTAAGGGATGCTATAGCACATAAAATGTATGTTGAAACATATGTA
AGGAATTACCATAGAATACATACACCATCAGGGGATACAGTGTTTAAAAGAATATATGAAGCTGCTGTTAACCATGGAATAGATCTATTAACAATGCTTA
TTGATAATACTGTTAAAATGGCTATGGAATTGAATGCATTCAATAGTTATGTAAATGTTGCAATAGATGAGCATGATGAGCCATATACTGGAAAAGACAA
CCCATATTTAATAGATGCACCATTCCATAGGTTCAGGGGCACAGACATGGCATACAGGTTTGCTACATTGGATTGTGTAGACAATAATAGATTTACTTTA
GCAGCTATGGTTAAGCATCCACTGGATGTAATTAATAATGCTAAGGAAGTTAGAATGGTTATAGAACATGCATTGTCACTGGGAATAAAGTAAATACAGT
ATTAATGGACAGGGGATACTTAGATTCCAGTGTAATGGATGCAGTGGACTCAATGAAATTGAAATATATTATACCTGCAAAGGACAATCATAAAGTTATA
AAATTCAAGAAAATGGATCTGAAATACTGCAAGGATGTATATGGAAATGAATTCCCATTTATGGTTTTAAGGGATAATATAGGATCATCAAAGAGAGTTA
ATGCCAATTTTGTCCATATAGTGTACTATTCCCATAAAAAGCATGATTTCTCATTCTATACCAATATTAATGTTAATGAAAACAATGTAATTGAACTTGC
AGAGCTGTACAGGAAAAGATGGGGAATAGAGAATGGATACCAGGAAAGGAAAATAGACATAAGGGAAAAGACACACTCAAGCCATATGGCTATAAGGCAA
TTCCTGTACTTCTTTTCCATACTGGTATACAATATATGGGTCCTTATCAACCTTATAAGGAGAATATATGGTTTATCACATATCATACTCATGGACTTTT
TAATATCCATGGGGAATAGGAAATGGAAAACAATAATAAATAACAATGGATAAATAAATAATAATTGCAGGGAAATATCCCGGTAATTATTGTATCATTA
GCTTAAGCTATGGATATTGTATTTTTAATATTCTTATAATACTGTAAAATTCATAATTTTTGTATCTTATAAAGCAACATTGGTGTATAATTCTGGTTGA
ATAGAAAATAAAATTATGCTTTATATTCATAAATTAATGATATTCTGCATCACTATCATAGACATGCGGAGAGTCTG
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence : No
  • Secondary structure : No-structure

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
573 bp190 aa121693+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MNNKTIPPKLNANKVKKFYSSQMYNIFSNNIHVQSRYNSGDIVNTVRDAIAHKMYVETYVRNYHRIHTPSGDTVFKRIYEAAVNHGIDLLTMLIDNTVKM
AMELNAFNSYVNVAIDEHDEPYTGKDNPYLIDAPFHRFRGTDMAYRFATLDCVDNNRFTLAAMVKHPLDVINNAKEVRMVIEHALSLGIK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
600 bp199 aa6541253+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

NGYRTCIVTGNKVNTVLMDRGYLDSSVMDAVDSMKLKYIIPAKDNHKVIKFKKMDLKYCKDVYGNEFPFMVLRDNIGSSKRVNANFVHIVYYSHKKHDFS
FYTNINVNENNVIELAELYRKRWGIENGYQERKIDIREKTHSSHMAIRQFLYFFSILVYNIWVLINLIRRIYGLSHIILMDFLISMGNRKWKTIINNNG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1133 bp377 aa1211253+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNNKTIPPKLNANKVKKFYSSQMYNIFSNNIHVQSRYNSGDIVNTVRDAIAHKMYVETYVRNYHRIHTPSGDTVFKRIYEAAVNHGIDLLTMLIDNTVKM
AMELNAFNSYVNVAIDEHDEPYTGKDNPYLIDAPFHRFRGTDMAYRFATLDCVDNNRFTLAAMVKHPLDVINNAKEVRMVIEHALSLGIKVNTVLMDRGY
LDSSVMDAVDSMKLKYIIPAKDNHKVIKFKKMDLKYCKDVYGNEFPFMVLRDNIGSSKRVNANFVHIVYYSHKKHDFSFYTNINVNENNVIELAELYRKR
WGIENGYQERKIDIREKTHSSHMAIRQFLYFFSILVYNIWVLINLIRRIYGLSHIILMDFLISMGNRKWKTIINNNG

 

Blast result :
Comments
ISFac1 is 48% aa similar to ISMma1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References