ISEch3
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AE005940 | ND | Erwinia chrysanthemi | Erwinia chrysanthemi 3937 |
DNA section
IS Length : 1363 bp
Ends
IR Length : 11
IRL : tgatgaaATGGACGCTCCAACTTGACGGCATCGAATGTGCCAAACTGAGA
IRR : ----tatATGGACGCTCCCACATTTGCAATCTGTTTTCTTTAAAACGGCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGATGAAATGGACGCTCCAACTTGACGGCATCGAATGTGCCAAACTGAGAGCATCACACTTCAGTTAAAGAAGGAGCGTCCACATGCAAGTATCAACTCT
TGGCATTGATTTGGCAAAAAATGTTTTCCAACTTCATGGCGTTGATAATTCAGGGAAAACCGTATTGCGAAAGCGACTGACCAGAAACGCATTCCTGCCA
TTTCTGGCCCAGTTGCCCCCCTGTTTAATTGGTATGGAAGCCTGTGCATCAGCTCATCACTTCGCTCGTCGTTTGATGCAGTACGGCCATCAGGTCAGGT
TGATGCCGCCCAGCTATGTCAAACCGTATGTGAAGCGGGATAAAACCGACGCGCGAGATGCAGAAGCCATTTGTGAAGCCGTAGCCCGACCCAACATGCG
TTTTGTTGCCGTGAAAAGTGAAACGCAGCAGGCCATATTAGCACTGCATACTGAACGGGACCTGCTGATTCGGGAAAGGGTTGCCAGCACCAACAGCTTG
CGGGCAATGCTGGCAGAGTTCGGTGTGGTGATGGAAACCGGCCTGTCAGCCTTGTACAAGACATTGCCGGTGATACTGGAGGATGGGGAAAATGCGCTCA
CGGCGCTGACAAGGCGCAGTGCAGCACGTCAACTAACGCATCTGAGGGAGCTGGAGCACCAGATAGCAGAAGTTGAACGTGAGTTAAGCGCGTGGTTCCA
CAGTCAGGAGGCCTGCAGGCGGCTGGAAAAAGTCCCTGGAGTTGGCATGCTGACGGCGACGTATATGGTTGCAGCAGTAGGGGATGGTTCACAGTTTACC
TCCTCAAAACAGTTTGCGGCGTGGGTGGGGCTGACGCCGAAGGAACACTCCAGTGGTGGTAAACAGTGGCTGGGCCCCATAAGTAAGCGGGGTGACCGTT
ATTTTCGCTATCTGCTGATACATGGAGCAAGAGCACTGGCGGCAGTGATAGAGCGACACCGTGATAAACAGACATGGTTTTATAGCCTGCTGAAGAGAAA
ACCGTTCAACGTGGCAGTGGTCGCCCAGGCAGGCAGAACAGCGAGAATCTTGTGGTCAATGTTGAGAGGCGGGAGTGAATACCGGGAAATCGCGCCTGTC
TGAAGGACGGGTTTTAAAGGAAGGAAACGAGTCAGGCGGTTCTGAAAAGTGCAAGGTGTAATCACAGTAATGGCAAAACAGGTAAGACCGCAGGAGAAAC
AGGTCGTTCGCCGCGTGGGCTTTGAGCCCGAAGGGATGTAAGACGTTCTCCTGGCGGATTTCATTATGGTGGCGCGACAAAAGCGAAAAACCGGATATAT
GACTGCACAACCTGCCATGCCGTTTTAAAGAAAACAGATTGCAAATGTGGGAGCGTCCATATA
TGGCATTGATTTGGCAAAAAATGTTTTCCAACTTCATGGCGTTGATAATTCAGGGAAAACCGTATTGCGAAAGCGACTGACCAGAAACGCATTCCTGCCA
TTTCTGGCCCAGTTGCCCCCCTGTTTAATTGGTATGGAAGCCTGTGCATCAGCTCATCACTTCGCTCGTCGTTTGATGCAGTACGGCCATCAGGTCAGGT
TGATGCCGCCCAGCTATGTCAAACCGTATGTGAAGCGGGATAAAACCGACGCGCGAGATGCAGAAGCCATTTGTGAAGCCGTAGCCCGACCCAACATGCG
TTTTGTTGCCGTGAAAAGTGAAACGCAGCAGGCCATATTAGCACTGCATACTGAACGGGACCTGCTGATTCGGGAAAGGGTTGCCAGCACCAACAGCTTG
CGGGCAATGCTGGCAGAGTTCGGTGTGGTGATGGAAACCGGCCTGTCAGCCTTGTACAAGACATTGCCGGTGATACTGGAGGATGGGGAAAATGCGCTCA
CGGCGCTGACAAGGCGCAGTGCAGCACGTCAACTAACGCATCTGAGGGAGCTGGAGCACCAGATAGCAGAAGTTGAACGTGAGTTAAGCGCGTGGTTCCA
CAGTCAGGAGGCCTGCAGGCGGCTGGAAAAAGTCCCTGGAGTTGGCATGCTGACGGCGACGTATATGGTTGCAGCAGTAGGGGATGGTTCACAGTTTACC
TCCTCAAAACAGTTTGCGGCGTGGGTGGGGCTGACGCCGAAGGAACACTCCAGTGGTGGTAAACAGTGGCTGGGCCCCATAAGTAAGCGGGGTGACCGTT
ATTTTCGCTATCTGCTGATACATGGAGCAAGAGCACTGGCGGCAGTGATAGAGCGACACCGTGATAAACAGACATGGTTTTATAGCCTGCTGAAGAGAAA
ACCGTTCAACGTGGCAGTGGTCGCCCAGGCAGGCAGAACAGCGAGAATCTTGTGGTCAATGTTGAGAGGCGGGAGTGAATACCGGGAAATCGCGCCTGTC
TGAAGGACGGGTTTTAAAGGAAGGAAACGAGTCAGGCGGTTCTGAAAAGTGCAAGGTGTAATCACAGTAATGGCAAAACAGGTAAGACCGCAGGAGAAAC
AGGTCGTTCGCCGCGTGGGCTTTGAGCCCGAAGGGATGTAAGACGTTCTCCTGGCGGATTTCATTATGGTGGCGCGACAAAAGCGAAAAACCGGATATAT
GACTGCACAACCTGCCATGCCGTTTTAAAGAAAACAGATTGCAAATGTGGGAGCGTCCATATA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1020 bp | 339 aa | 84 | 1103 | + | No |
Chemistry : DEDD
ORF sequence :
MQVSTLGIDLAKNVFQLHGVDNSGKTVLRKRLTRNAFLPFLAQLPPCLIGMEACASAHHFARRLMQYGHQVRLMPPSYVKPYVKRDKTDARDAEAICEAV
ARPNMRFVAVKSETQQAILALHTERDLLIRERVASTNSLRAMLAEFGVVMETGLSALYKTLPVILEDGENALTALTRRSAARQLTHLRELEHQIAEVERE
LSAWFHSQEACRRLEKVPGVGMLTATYMVAAVGDGSQFTSSKQFAAWVGLTPKEHSSGGKQWLGPISKRGDRYFRYLLIHGARALAAVIERHRDKQTWFY
SLLKRKPFNVAVVAQAGRTARILWSMLRGGSEYREIAPV
ARPNMRFVAVKSETQQAILALHTERDLLIRERVASTNSLRAMLAEFGVVMETGLSALYKTLPVILEDGENALTALTRRSAARQLTHLRELEHQIAEVERE
LSAWFHSQEACRRLEKVPGVGMLTATYMVAAVGDGSQFTSSKQFAAWVGLTPKEHSSGGKQWLGPISKRGDRYFRYLLIHGARALAAVIERHRDKQTWFY
SLLKRKPFNVAVVAQAGRTARILWSMLRGGSEYREIAPV
Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. Unlike most IS1111 family elements, the first nt of the sequence shown (T) does not match the 4th nt to the right of IRr (a C not shown in the above sequence). There are 3 complete copies of this IS in the Erwinia chrysanthemi 3937 genome and one of the other copies has T at both positions, while the other has C.
The transposase is 69% identical to that of ISYen1 and 41% identical to that of IS1111.
By analogy with IS4321, ISEch3 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region locatedjust inside the right-hand end of the element are correctly spaced to form a promoter.
The transposase is 69% identical to that of ISYen1 and 41% identical to that of IS1111.
By analogy with IS4321, ISEch3 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region locatedjust inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] The Institute for Genomic Research http://www.tigr.org
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384