ISAfe1

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acidithiobacillus ferroxidans
Acidithiobacillus ferroxidans ATCC 23270
DNA section
IS Length : 1424 bp

Ends


IR Length : 12

IRL : agatgtaATGGATGCCTCCCCCTACAGGACCGGCAGCGGGGTGCTTCAGA
IRR : ----tatATGGATGCCTCCATGTGTGCAAGAGGATTGCGATGTCTGACAT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

AGATGTAATGGATGCCTCCCCCTACAGGACCGGCAGCGGGGTGCTTCAGAAGGGAGTCCCCGCTTGATCCGGACGGCATCAAGGTGCCAAGGTGGAAATT
TATCCACGTAAAGGACAAGGAGGACTCGTCATGCAGGTTACAACATACGGATTGGATTTGGCAAAAAGGGTCATGCAACTGCACTGGGTAAACCTGGAGA
CCGGCGAGATTCACCGCAAACAGTTGAAGCGCCGTGCACTCCTGGAGTTTTTCGCCAACCGCCAACCGGGAATCGTGGCGATGGAGGCTTGCGGTAGCGC
CCACTATTGGGGACGGGAATTGCGTAAGCTGGGGCATGAGGTCCGCTTGATTGCTGCCCAGTTTGTGCGTCCCTTCGTGAAGACCAACAAGAACGATGCG
GCCGATGCGGCAGCGATCTGGGAAGCCGTACAGCGGCCTGATATGCGCTTCGTGGCGGTCAAGAGTGAGGAACAGCAATCCGTTCTGTCGCTGCACCGTA
TGCGGGAGCAATTGATCAAGATGCGCACTATGCAGGTGAACCAGATCCGGGGCCTGCTCTACGAATTTGGTGCTGATCTGCCGCAGGGCCGCCAGCGGGG
GCTGAAAGAGGTTCCTGATGCTTTGGCCGACCTGGAAAAATCGATATCGCCCATGCTGCGGGACACGGTTCGTCAGCAATTAAAACGTCTTGAAGAGATG
GACAAGGATATTGCGGATATCGAAAAGCGTTTGACGCTGTGGAAGAAAGGCCAAGAGGCCGTGACTCGATTGATGGCTATCCCCGGCGTGGGGCTACTTA
CGGCAACTGCCATTATCGCCACCGTGGGAGATATGAAGTCCTTTCGTTCAGGACGGGAGTTTGCGGCGTTTCTGGGGCTGGTTCCCCGCCAGAGTGGGAC
CGGTGGCAAGGTCCGATTACTGGGGATCAGCAAACGGGGGGATACCTATCTCCGAACATTGCTGACCCATGGAGCCAGGGCGGTGGTGAACTTCCAGATC
AAGAATCGGAATCCCTGGATTGACAAACTGCTCAGTCGGCGACCCCATAATGTGGTGGTCGTGGCACAGTCCAACAAGATGGCGCGAACCATCTGGGCGC
TGCTGGTGAAAAACACAGAATATGTGGCCAATCACGCTAGGAATGCGGCCGCCCTATAGTAACAGTTACAGGATTGTTGACGTTCAGCAAAAGGAGTTGC
GCAGGCGGTACACGATTGATGGCAAAACAGGTCAGACCGCAGGAAAGGGAACCTGGAAGCTCTCTGTCCTAATAAGGACGCTTTAGAGATGAGGACTTTC
CTGGCGGATTTCATCAGGGCCAGCAGGCTTAATGTCCTGCATCAAAGGCCGGATATAAGACTGCAACCCGACCTCAACATGTCAGACATCGCAATCCTCT
TGCACACATGGAGGCATCCATATA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1029 bp342 aa1311159+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MQVTTYGLDLAKRVMQLHWVNLETGEIHRKQLKRRALLEFFANRQPGIVAMEACGSAHYWGRELRKLGHEVRLIAAQFVRPFVKTNKNDAADAAAIWEAV
QRPDMRFVAVKSEEQQSVLSLHRMREQLIKMRTMQVNQIRGLLYEFGADLPQGRQRGLKEVPDALADLEKSISPMLRDTVRQQLKRLEEMDKDIADIEKR
LTLWKKGQEAVTRLMAIPGVGLLTATAIIATVGDMKSFRSGREFAAFLGLVPRQSGTGGKVRLLGISKRGDTYLRTLLTHGARAVVNFQIKNRNPWIDKL
LSRRPHNVVVVAQSNKMARTIWALLVKNTEYVANHARNAAAL

 

Blast result :
Comments
No uninterrupted target sequence was found, so the ends of the IS have been defined by analogy with other IS1111 family elements, assuming that 7 nt separate IRl from the left-hand end of the element and 4 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.

There are 2 copies of ISAfe1 in the Acidithiobacillus ferroxidans ATCC 23270 genome and the transposase protein is 42% identical to that of IS1111.
References
1] The Institute for Genomic Research http://www.tigr.org
2] Partridge and Hall (2003) J. Bacteriol. 185 6371-6384