ISBfun1
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Burkholderia fungorum | Burkholderia fungorum LB400 |
DNA section
IS Length : 1466 bp
Ends
IR Length : 12/13
IRL : gaatgaAAGGGACTTCCCCGTCCCGAGGCTGCGGCGGAAGCCGCGAGTCG
IRR : ---tacAAGGG-CTTCCCCGTTGAGCACAAGCTCAATCGAGGTTTGCATG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GAATGAAAGGGACTTCCCCGTCCCGAGGCTGCGGCGGAAGCCGCGAGTCGGCATCAATGTGCCAAGATGAAGTTGCGAACGTTCATCGACACCAGCGAGA
GGGGAAGTCCATGCCTATTGTCACTATCGGAATCGATCTTGCCAAGAATATATTCGCGGTTCACGGTGTGGACGAAGCCGGTAAAGCGATGCTGATCAAG
CCACGTGTGCCGCGTGATCAGCTGGCGACGTTGATTGCGCAGTTGCCAGCGTGCCTCATCGGCATGGAGGCCTGCTCCGGTGCGCATCACTGGGCCCGCC
TCTTCCAGCAGCATGGCCATACCGTCAAGCTCATGGCGCCCAACCTTGTGGCGCCTTATCGCATGTCGGGCAAGCGAGGCAAGAACGACGCGGCGGATGC
TGCTGCGATCTGCGAAGCCGTGGCGCGCCCAAGCATGCGTTTCGTGCCGCTGAAGGACGAACATCAGCAGGCAACGCTTTGCCTGCACCGGACACGACAA
GGTTTCGTTGAAGAGCGAACCGCGACCTACAACCGGTTGCGAGGCCTGCTCCCGGAATTCGGTGTAGTGCTACCCCAGAGCCCGGAGAAGTTGCGTAGGT
ACGTCACGGAACACCTGGACGCATTGCCAGGCTGGGCGAAGCGTAGCATCGGCGATCTGCTTGAGCATGCCGGCCATATCGAGGATCGCCTGCTCGAATA
CGACCGTGCGATCGCTGAGATCGCGCGTGAGGATGCGCGCAGCAAGAGACTGATGCAGTTGCGCGGCGTCGGCCCAACCACGGCCAGCGCGTTGCTCGCC
AGTATCGGTGCCGGGCGCGACTTCAGAAACGGCCGACAGGTAGCAGCCTGGATCGGACTCACGCCCGGTCAATACAGCAGTGGCGGCAAGGCGCGGCTGG
GCAGCATCACGAAGGCAGGCGACGCCTATCTGCGCGGGTTGCTGGTCAACGGTGCCCGCGCCGTCATTGCCAATCTCGGCGAGAAACAGGATCGTTTCAG
CCGTTGGGTCCGAACCCTGATCGAGCGCCGTGGCTACTGGCGAGCTGCTGTCGCGATTGCCGCAAAGAACGCGAGATTGGCTTGGGCCTCGTTGAAATAT
GGCGAGGACTTCAAGCTCGAACCTGCGGCAGACTGAGTTCGATTCGAGCCTGTCGATCTACCAATGCCTGGACGCGAGCACGGACATCATGAGCACATAG
AAGCGATCACTTTGCACTGCCAGCGGTGATGTGAAGCGGGTTGGACCCGCGCGGGGCGTACCTGGCTAACACGGCGGGGATATCCATTCCCGGCTAGAGA
ATGAGACCCCTGCGCGCGTCTTTCATCAGGGTCCGGGCCATTGGTCCAACATGACCGGTTGTAGAACCGCAGTCCTTCACCTTCTCACATACACCAGCGC
GGCATTGCAGCATGTATCGACATGCAAACCTCGATTGAGCTTGTGCTCAACGGGGAAGCCCTTGTA
GGGGAAGTCCATGCCTATTGTCACTATCGGAATCGATCTTGCCAAGAATATATTCGCGGTTCACGGTGTGGACGAAGCCGGTAAAGCGATGCTGATCAAG
CCACGTGTGCCGCGTGATCAGCTGGCGACGTTGATTGCGCAGTTGCCAGCGTGCCTCATCGGCATGGAGGCCTGCTCCGGTGCGCATCACTGGGCCCGCC
TCTTCCAGCAGCATGGCCATACCGTCAAGCTCATGGCGCCCAACCTTGTGGCGCCTTATCGCATGTCGGGCAAGCGAGGCAAGAACGACGCGGCGGATGC
TGCTGCGATCTGCGAAGCCGTGGCGCGCCCAAGCATGCGTTTCGTGCCGCTGAAGGACGAACATCAGCAGGCAACGCTTTGCCTGCACCGGACACGACAA
GGTTTCGTTGAAGAGCGAACCGCGACCTACAACCGGTTGCGAGGCCTGCTCCCGGAATTCGGTGTAGTGCTACCCCAGAGCCCGGAGAAGTTGCGTAGGT
ACGTCACGGAACACCTGGACGCATTGCCAGGCTGGGCGAAGCGTAGCATCGGCGATCTGCTTGAGCATGCCGGCCATATCGAGGATCGCCTGCTCGAATA
CGACCGTGCGATCGCTGAGATCGCGCGTGAGGATGCGCGCAGCAAGAGACTGATGCAGTTGCGCGGCGTCGGCCCAACCACGGCCAGCGCGTTGCTCGCC
AGTATCGGTGCCGGGCGCGACTTCAGAAACGGCCGACAGGTAGCAGCCTGGATCGGACTCACGCCCGGTCAATACAGCAGTGGCGGCAAGGCGCGGCTGG
GCAGCATCACGAAGGCAGGCGACGCCTATCTGCGCGGGTTGCTGGTCAACGGTGCCCGCGCCGTCATTGCCAATCTCGGCGAGAAACAGGATCGTTTCAG
CCGTTGGGTCCGAACCCTGATCGAGCGCCGTGGCTACTGGCGAGCTGCTGTCGCGATTGCCGCAAAGAACGCGAGATTGGCTTGGGCCTCGTTGAAATAT
GGCGAGGACTTCAAGCTCGAACCTGCGGCAGACTGAGTTCGATTCGAGCCTGTCGATCTACCAATGCCTGGACGCGAGCACGGACATCATGAGCACATAG
AAGCGATCACTTTGCACTGCCAGCGGTGATGTGAAGCGGGTTGGACCCGCGCGGGGCGTACCTGGCTAACACGGCGGGGATATCCATTCCCGGCTAGAGA
ATGAGACCCCTGCGCGCGTCTTTCATCAGGGTCCGGGCCATTGGTCCAACATGACCGGTTGTAGAACCGCAGTCCTTCACCTTCTCACATACACCAGCGC
GGCATTGCAGCATGTATCGACATGCAAACCTCGATTGAGCTTGTGCTCAACGGGGAAGCCCTTGTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1026 bp | 341 aa | 111 | 1136 | + | No |
Chemistry : DEDD
ORF sequence :
MPIVTIGIDLAKNIFAVHGVDEAGKAMLIKPRVPRDQLATLIAQLPACLIGMEACSGAHHWARLFQQHGHTVKLMAPNLVAPYRMSGKRGKNDAADAAAI
CEAVARPSMRFVPLKDEHQQATLCLHRTRQGFVEERTATYNRLRGLLPEFGVVLPQSPEKLRRYVTEHLDALPGWAKRSIGDLLEHAGHIEDRLLEYDRA
IAEIAREDARSKRLMQLRGVGPTTASALLASIGAGRDFRNGRQVAAWIGLTPGQYSSGGKARLGSITKAGDAYLRGLLVNGARAVIANLGEKQDRFSRWV
RTLIERRGYWRAAVAIAAKNARLAWASLKYGEDFKLEPAAD
CEAVARPSMRFVPLKDEHQQATLCLHRTRQGFVEERTATYNRLRGLLPEFGVVLPQSPEKLRRYVTEHLDALPGWAKRSIGDLLEHAGHIEDRLLEYDRA
IAEIAREDARSKRLMQLRGVGPTTASALLASIGAGRDFRNGRQVAAWIGLTPGQYSSGGKARLGSITKAGDAYLRGLLVNGARAVIANLGEKQDRFSRWV
RTLIERRGYWRAAVAIAAKNARLAWASLKYGEDFKLEPAAD
Blast result :
Comments
No uninterrupted target sequence was found, so the ends of the IS have been defined by analogy with closely related IS1111 family elements, assuming that 6 nt separate IRl from the left-hand end of the element and 4 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 5 nt on the left and 4 on the right.
The transposase protein is 81, 80 and 39% identical to those of IS1492, ISPsy16 and IS1111. respectively.
The transposase protein is 81, 80 and 39% identical to those of IS1492, ISPsy16 and IS1111. respectively.
References
1] US Department of Energy Joint Genome Institute http://jgi.doe.gov/JGI_microbial/html/
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384