ISEch4
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Erwinia chrysanthemi | Erwinia chrysanthemi 3937 |
DNA section
IS Length : 1354 bp
Ends
IR Length : 12
IRL : taataagATGGTCACCCCCACCCTGTGAGCGGTACGCTGACAGGGTGTTT
IRR : ----tctATGGTCACCCCCGTTTTTGCAACACCGATTTTGATTTATGATG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TAATAAGATGGTCACCCCCACCCTGTGAGCGGTACGCTGACAGGGTGTTTTTCTTTCTGAAGGGGCTCATCATGCAAAACGTTACGCTCATCGGTATCGA
CCTCGGCAAGCATTCGTTCCATGTCCACGCTCAGGATAAAAACGGCAATGCATTGCTACGTAAAAAGTTTTCTCGCAACCAGTTAACCGCCTTTCTCTCT
ACTTGTACCGCTTCTACCGTTGTTATGGAGTCCTGTGCCGGCGCTCATTTTATGGCACGACACATCAGCCAGTTCGGTCATCAGGTGAAGCTCATCTCTC
CTCAGTTTGTCCGACCCTTTGTTAAAAGCAACAAGAACGATTTCATTGATGCTGAGGCTATCTGCGAAGCCGCCTCACGACCTTCTATGCGTTTCGTCAC
CCCGCGAACCGAGGACCAGCAGGCTATGTCAGCGTTCCATCGCGTCCGTGACACTCTCGTCAGAGAACGCGTTAAAACGACCAATCAGATGCATGCTTTC
CTGCTGGAGTTTGGCATTAGCATGCCGCGCGGCATCGCGATTATCAGGCGTCTTGCCGTAGTGCTTGAAGAGCACGAACTGCCGCCTTATTTAGCGCGAT
TGCTTATGCGACTCCATCAGCATTATGCCTGCCTCACCGAACAGATTAAGGAGCTCGAACAAGAGTTAAAAATCCACATCGCGGGTGACGATACCGCGCA
GCGCCTGCTGACTATCCCCGGCGTCGGGCTTATCACGGCCAGCCTTTTAGCCACGAAGCTCGGCGACGGAAAAAGTTATGCCAGCAGCCGGGACTTTGCT
GCCTCAACGGGGTTAGTCCCGCGTCAGTACAGCACGGGCGGGAAAAGTACGCTAATGGGCATCAGTAAGCGCGGGGATAAAAATCTGCGGCGTCTTCTGG
TGCAATGCGCTCGCGCTTTTATGCAACGGCTTGAACACAATCAGGGACGGCTGGCTGACTGGGTGAAGACCCAGCTCTCGCGGCATCACTCGAACGTGGT
GGCCTGCGGGCTGGCGAACAAATTAGCCCGAATAGCCTGGTCGGTGACAACCCATCGGACAGCGTTTATCCAATAAAAAAGAGCCAACAGCGACGAACAG
TTAGCTCTTGGGTGTTACGCAGTGACTTTCTGGTTTTGCGACGACTGATTATTGATGACATGAACGGCACAGCGACCTGGGGATGAACCTGACATAAAAA
ATGGCTCTTTGAAGCCGTCCGGTTTTTAAGGTTCTGCAGGCGCGGACTTCATCGTGGCGCGGGCAACGTAAGGTTGCCCATCAGACGCCGTATAGATTTA
AGCAAGCCCATCATAAATCAAAATCGGTGTTGCAAAAACGGGGGTGACCATAGA
CCTCGGCAAGCATTCGTTCCATGTCCACGCTCAGGATAAAAACGGCAATGCATTGCTACGTAAAAAGTTTTCTCGCAACCAGTTAACCGCCTTTCTCTCT
ACTTGTACCGCTTCTACCGTTGTTATGGAGTCCTGTGCCGGCGCTCATTTTATGGCACGACACATCAGCCAGTTCGGTCATCAGGTGAAGCTCATCTCTC
CTCAGTTTGTCCGACCCTTTGTTAAAAGCAACAAGAACGATTTCATTGATGCTGAGGCTATCTGCGAAGCCGCCTCACGACCTTCTATGCGTTTCGTCAC
CCCGCGAACCGAGGACCAGCAGGCTATGTCAGCGTTCCATCGCGTCCGTGACACTCTCGTCAGAGAACGCGTTAAAACGACCAATCAGATGCATGCTTTC
CTGCTGGAGTTTGGCATTAGCATGCCGCGCGGCATCGCGATTATCAGGCGTCTTGCCGTAGTGCTTGAAGAGCACGAACTGCCGCCTTATTTAGCGCGAT
TGCTTATGCGACTCCATCAGCATTATGCCTGCCTCACCGAACAGATTAAGGAGCTCGAACAAGAGTTAAAAATCCACATCGCGGGTGACGATACCGCGCA
GCGCCTGCTGACTATCCCCGGCGTCGGGCTTATCACGGCCAGCCTTTTAGCCACGAAGCTCGGCGACGGAAAAAGTTATGCCAGCAGCCGGGACTTTGCT
GCCTCAACGGGGTTAGTCCCGCGTCAGTACAGCACGGGCGGGAAAAGTACGCTAATGGGCATCAGTAAGCGCGGGGATAAAAATCTGCGGCGTCTTCTGG
TGCAATGCGCTCGCGCTTTTATGCAACGGCTTGAACACAATCAGGGACGGCTGGCTGACTGGGTGAAGACCCAGCTCTCGCGGCATCACTCGAACGTGGT
GGCCTGCGGGCTGGCGAACAAATTAGCCCGAATAGCCTGGTCGGTGACAACCCATCGGACAGCGTTTATCCAATAAAAAAGAGCCAACAGCGACGAACAG
TTAGCTCTTGGGTGTTACGCAGTGACTTTCTGGTTTTGCGACGACTGATTATTGATGACATGAACGGCACAGCGACCTGGGGATGAACCTGACATAAAAA
ATGGCTCTTTGAAGCCGTCCGGTTTTTAAGGTTCTGCAGGCGCGGACTTCATCGTGGCGCGGGCAACGTAAGGTTGCCCATCAGACGCCGTATAGATTTA
AGCAAGCCCATCATAAATCAAAATCGGTGTTGCAAAAACGGGGGTGACCATAGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1005 bp | 334 aa | 72 | 1076 | + | No |
Chemistry : DEDD
ORF sequence :
MQNVTLIGIDLGKHSFHVHAQDKNGNALLRKKFSRNQLTAFLSTCTASTVVMESCAGAHFMARHISQFGHQVKLISPQFVRPFVKSNKNDFIDAEAICEA
ASRPSMRFVTPRTEDQQAMSAFHRVRDTLVRERVKTTNQMHAFLLEFGISMPRGIAIIRRLAVVLEEHELPPYLARLLMRLHQHYACLTEQIKELEQELK
IHIAGDDTAQRLLTIPGVGLITASLLATKLGDGKSYASSRDFAASTGLVPRQYSTGGKSTLMGISKRGDKNLRRLLVQCARAFMQRLEHNQGRLADWVKT
QLSRHHSNVVACGLANKLARIAWSVTTHRTAFIQ
ASRPSMRFVTPRTEDQQAMSAFHRVRDTLVRERVKTTNQMHAFLLEFGISMPRGIAIIRRLAVVLEEHELPPYLARLLMRLHQHYACLTEQIKELEQELK
IHIAGDDTAQRLLTIPGVGLITASLLATKLGDGKSYASSRDFAASTGLVPRQYSTGGKSTLMGISKRGDKNLRRLLVQCARAFMQRLEHNQGRLADWVKT
QLSRHHSNVVACGLANKLARIAWSVTTHRTAFIQ
Blast result :
Comments
No uninterrupted target sequence was found, so the ends of the IS have been defined by analogy with other IS1111 family elements, assuming that 7 nt separate IRl from the left-hand end of the element and 4 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
There are 4 copies of this IS in the Erwinia chrysanthemi 3937 genome. The transposase protein is 82% identical to that of IS1328 and 37% identical to that of IS1111.
There are 4 copies of this IS in the Erwinia chrysanthemi 3937 genome. The transposase protein is 82% identical to that of IS1328 and 37% identical to that of IS1111.
References
1] The Institute for Genomic Research http://www.tigr.org
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384