ISBj4
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AP005952 | ND | Bradyrhizobium japonicum | Bradyrhizobium japonicum USDA110 |
DNA section
IS Length : 1576 bp
Ends
IR Length : 14/15
IRL : taatgggATGGTCCGGCCGTGCTCCTGCCCCGCCAGCGATAAGCTGGCCA
IRR : ----tgtACGGTCCGGCCGTGCGTAGCAAGAGGTTCGTCGATCAGGTGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TAATGGGATGGTCCGGCCGTGCTCCTGCCCCGCCAGCGATAAGCTGGCCAGGGGCGCCAAGACAAGGAGCACGCCATGTCCCAGACACCCAGTACCGCGA
TCGCCGTGATCGGCATCGATATCGGCAAAAACTCGTTCCACGTCGTGGGCCACGATACGCGCGGCACCGTCGTGCTGCGGCAAAAGTGGTCGCGTGGCCA
GGTCGAGGCGCGGCTCGCCAATATGCCGCCTTGCCTGATCGGCATGGAAGCCTGCGTCGGCGCACATCATTTGAGCCGCAAACTCGCATCGTTAGGTCAC
GATGCCAGGTTGATGCCGGCCAAATATGTCCGCCCTTATAGCAAAGGACCGAAGAACGACTTCAATGACGCCGAAGCGATTGCCGAAGCCGTGCAGCGCC
CGACGATGAAGTTCGTGGCGACCAAGACCGCGGAGCAACTGGATCTGCAGGCGCTGCATCGGGTGCGCGAGCGGCTGGTGTCGCAACGCACCGGCATCAT
CAACCAGATTCGCGCCTTCATGCTGGAACGCGGGATCGCGGTGCGCCAGGGTATCGGCTTCCTGCGCACAGAACTGCCCACCATCCTTGCGACGCGCACC
GATGCCCTGTCGCCACGCATGTTGTGTGTCATCGAGGAGTTGGCAGGCGACTGGCGTCGGCTGGATCAGCGCATCGATAGGCTCTCCGGCGAGATCGAAG
CACTGGCCCGTCAAGATCAGGCATGTTCGCGCCTGATGACGGTGCCTGGCATCGGGCCGATCATTTCGAGCGCCATGGTGGCCGCGATCGGCACTGGAGA
CGTCTTCTCCAAAGGCCGTGACTTCGGCGCCTGGCTCGGGCTGGTGCCCAAGCAGATCTCGACGGGAGACCGCACGATCCTCGGCAAAATCTCAAGGCGC
GGCAATCGCTACCTCCGCGTTCTGTTCGTCCAGGCGGCATGGGTGGTGCTGGTCAGGATCAAGAACTGGGAACGTTACGGACTCAGATCCTGGATCGAAG
CCGCCAAGAAGCGATTGCACCACAACGTGCTGGCGATCGCACTCGCCAACAAGCTTGCCCGCATCGCCTGGGCGGTGCTGGCTAAAGGACGCGCCTTCGA
GCTGACAAGGACCAACGATGCAGGCGTCCGACCCGCTTGATCGTCGCGCCGTGCTCGGCGCGGTCAAGGCGCGGCCTGGCAACGCCGGAGCCAGCCGCAA
GCAAAGCGCAACGGCCGGCCTTGACCGCCCGTGCGCGCGACGCGACGCACGTCCTGCGGGCCGGGACGAAGGAACGGCCCCTGGCTCGAACACAGGAACT
GCGAGGTAGGAGGAGCAAGCGATGACGTAACCCCATCAACAGGTTCCAGCCGAGGTCTGCGAGAGGATGAGACGAGATGGAGGTTCGGTCTTCCCGGCGC
ATGCGAACACTGGTGACCCAAATGGCCCAATCGAGGCCTGTCCGCTAATGAGAACGCACGCGCGCTGATATCCATGATGGCCCGGAGAAAATGCTCCAAT
CAAAGGCCGGATACATTGATGCAAGACCACACCACCTGATCGACGAACCTCTTGCTACGCACGGCCGGACCGTACA
TCGCCGTGATCGGCATCGATATCGGCAAAAACTCGTTCCACGTCGTGGGCCACGATACGCGCGGCACCGTCGTGCTGCGGCAAAAGTGGTCGCGTGGCCA
GGTCGAGGCGCGGCTCGCCAATATGCCGCCTTGCCTGATCGGCATGGAAGCCTGCGTCGGCGCACATCATTTGAGCCGCAAACTCGCATCGTTAGGTCAC
GATGCCAGGTTGATGCCGGCCAAATATGTCCGCCCTTATAGCAAAGGACCGAAGAACGACTTCAATGACGCCGAAGCGATTGCCGAAGCCGTGCAGCGCC
CGACGATGAAGTTCGTGGCGACCAAGACCGCGGAGCAACTGGATCTGCAGGCGCTGCATCGGGTGCGCGAGCGGCTGGTGTCGCAACGCACCGGCATCAT
CAACCAGATTCGCGCCTTCATGCTGGAACGCGGGATCGCGGTGCGCCAGGGTATCGGCTTCCTGCGCACAGAACTGCCCACCATCCTTGCGACGCGCACC
GATGCCCTGTCGCCACGCATGTTGTGTGTCATCGAGGAGTTGGCAGGCGACTGGCGTCGGCTGGATCAGCGCATCGATAGGCTCTCCGGCGAGATCGAAG
CACTGGCCCGTCAAGATCAGGCATGTTCGCGCCTGATGACGGTGCCTGGCATCGGGCCGATCATTTCGAGCGCCATGGTGGCCGCGATCGGCACTGGAGA
CGTCTTCTCCAAAGGCCGTGACTTCGGCGCCTGGCTCGGGCTGGTGCCCAAGCAGATCTCGACGGGAGACCGCACGATCCTCGGCAAAATCTCAAGGCGC
GGCAATCGCTACCTCCGCGTTCTGTTCGTCCAGGCGGCATGGGTGGTGCTGGTCAGGATCAAGAACTGGGAACGTTACGGACTCAGATCCTGGATCGAAG
CCGCCAAGAAGCGATTGCACCACAACGTGCTGGCGATCGCACTCGCCAACAAGCTTGCCCGCATCGCCTGGGCGGTGCTGGCTAAAGGACGCGCCTTCGA
GCTGACAAGGACCAACGATGCAGGCGTCCGACCCGCTTGATCGTCGCGCCGTGCTCGGCGCGGTCAAGGCGCGGCCTGGCAACGCCGGAGCCAGCCGCAA
GCAAAGCGCAACGGCCGGCCTTGACCGCCCGTGCGCGCGACGCGACGCACGTCCTGCGGGCCGGGACGAAGGAACGGCCCCTGGCTCGAACACAGGAACT
GCGAGGTAGGAGGAGCAAGCGATGACGTAACCCCATCAACAGGTTCCAGCCGAGGTCTGCGAGAGGATGAGACGAGATGGAGGTTCGGTCTTCCCGGCGC
ATGCGAACACTGGTGACCCAAATGGCCCAATCGAGGCCTGTCCGCTAATGAGAACGCACGCGCGCTGATATCCATGATGGCCCGGAGAAAATGCTCCAAT
CAAAGGCCGGATACATTGATGCAAGACCACACCACCTGATCGACGAACCTCTTGCTACGCACGGCCGGACCGTACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1065 bp | 354 aa | 76 | 1140 | + | No |
Chemistry : DEDD
ORF sequence :
MSQTPSTAIAVIGIDIGKNSFHVVGHDTRGTVVLRQKWSRGQVEARLANMPPCLIGMEACVGAHHLSRKLASLGHDARLMPAKYVRPYSKGPKNDFNDAE
AIAEAVQRPTMKFVATKTAEQLDLQALHRVRERLVSQRTGIINQIRAFMLERGIAVRQGIGFLRTELPTILATRTDALSPRMLCVIEELAGDWRRLDQRI
DRLSGEIEALARQDQACSRLMTVPGIGPIISSAMVAAIGTGDVFSKGRDFGAWLGLVPKQISTGDRTILGKISRRGNRYLRVLFVQAAWVVLVRIKNWER
YGLRSWIEAAKKRLHHNVLAIALANKLARIAWAVLAKGRAFELTRTNDAGVRPA
AIAEAVQRPTMKFVATKTAEQLDLQALHRVRERLVSQRTGIINQIRAFMLERGIAVRQGIGFLRTELPTILATRTDALSPRMLCVIEELAGDWRRLDQRI
DRLSGEIEALARQDQACSRLMTVPGIGPIISSAMVAAIGTGDVFSKGRDFGAWLGLVPKQISTGDRTILGKISRRGNRYLRVLFVQAAWVVLVRIKNWER
YGLRSWIEAAKKRLHHNVLAIALANKLARIAWAVLAKGRAFELTRTNDAGVRPA
Blast result :
Comments
No uninterrupted target sequence was found, so the ends of the IS have been defined by analogy with other IS1111 family elements, assuming that 7 nt separate IRl from the left-hand end of the element and 4 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
There are 6 copies in the Bradyrhizobium japonicum USDA110 genome, with the transposase gene corresponding to orfs bll4626, blr5195, blr6054, bll7554, blr7715 and bll8183.
The transposase protein is 37% identical to that of IS1111.
There are 6 copies in the Bradyrhizobium japonicum USDA110 genome, with the transposase gene corresponding to orfs bll4626, blr5195, blr6054, bll7554, blr7715 and bll8183.
The transposase protein is 37% identical to that of IS1111.
References
1] Kaneko et al. (2002) DNA Res. 9, 189-197 and 225-256
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384