ISSod14
- Family IS110
- Group IS1111
Isoform Synonym(s) ISSo14
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AE015826 | ND | Shewanella oneidensis | Shewanella oneidensis MR-1 |
DNA section
IS Length : 1438 bp
Ends
IR Length : 13
IRL : tgctgagATGGTCACCTCGGTCTCGGCATCGCAATGTGCCATGATTTAGG
IRR : ----tatATGGTCACCTCGGGGTTAGCAAGGTCAATGTCCTATTTTGTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGCTGAGATGGTCACCTCGGTCTCGGCATCGCAATGTGCCATGATTTAGGTGCACAAAATCTGACTCGAGGTGACCGATATGAACTATAAACTCATCAGC
ATTGATTTGGCAAAAACTGTCTTCCAAGTCGCAGCCTTTAACCCTGATAACACCATTGCTTTTAACCGTAAAATCAATCGCCCAGCATTACTTGACATGC
TTAGGCAATGCGAGCCGACACTCGTGGTGATGGAAGCCTGCTACAGCGCTAATTACTGGGGGCGTACAATCGAAAAACTCGGCCATACCGTCAAGCTTAT
CCCCGCCAGAATGGTGAAAGCCATGCTGGTTGGTAATAAGAATGACGCCAATGATGCGGTTGCCATCGGAGAAACGGCTGGCCGCCCTAAAGTGAGTCTA
CTTCGCCCTAAAACGGTCGAGCAGCAGGATATTCAATCTTTACTGCGCATTCGTGAGCGCTTGGTGGAGAATCGCACGGCTAACTGCAATCAACTGCGTG
GTTTTCTCGCGGAATACGGCCTGATTGTCGCAAAGACCCGCCGCCAGTTGATGTTAGCCATTCCGCTCATTCTTGAAGATGCTGACAACGAATTATCCAC
GGTTGCCAGAGGGTTTATTCAACGACTCTATGAGTTTCAGTTGTATTTAAATGAACAAATCGACCAGGTTGCACAGGACATATTAGCCCTTGCCCAAGCC
ATGCCACAGTATCCATTATTGCTAACCATCCCCGGTGTCGGCCCTATCGTCGCATCCACATTATTAGCCAGTATTAATGAAGTTAATGATTTTAAAAATG
GCCGGCAATTGGCGGCATGGCTAGGGCTCACTCCTTCACAATACAGCAGTGGCACGACCAATCGGCTCGGCAAGATAACCAAGCGCGGAAATCAAACGCT
CCGGCGGTTACTTATCCATGGCGCAAGAACCGTGGTGAACTGGTGCGATAAGAAAGACGACTCACTTAGCCTGTGGTTACAACGGCTTAAACAGCGTCAA
CACGTCTGCAAAGTGACGGTTGCCTTGGCCAATAAGATGGCTCGGATGGTGTTTGTAGTGATGAGCCGCGGACAAGCATTTGATATGAATAAGGCCTGTA
AATTGGCAACGTAATGCAGTGTTTTTTGCGTAAATAGACTCAAAATGTAAATAGATAAATGTCCATCAAAGCACAACAGCGGTAATGGTATTAATGAGTT
AAACGGTTTGTCTTACCCAAAACCTGATGAGGACAGTGGCCTTTGAGGCCATGTGGGTGCCAAGGAGGGTAAGACGTCAATTTGGATCTCATTAGGGCGC
AACAGACGCCGGATATATGTCGCAAGACCGCAAACCATTAACGACCGATACCCCGTTGTGAAAGGTGAATTATCGATTAGCACAAATTAATTTGAACAAA
ATAGGACATTGACCTTGCTAACCCCGAGGTGACCATATA
ATTGATTTGGCAAAAACTGTCTTCCAAGTCGCAGCCTTTAACCCTGATAACACCATTGCTTTTAACCGTAAAATCAATCGCCCAGCATTACTTGACATGC
TTAGGCAATGCGAGCCGACACTCGTGGTGATGGAAGCCTGCTACAGCGCTAATTACTGGGGGCGTACAATCGAAAAACTCGGCCATACCGTCAAGCTTAT
CCCCGCCAGAATGGTGAAAGCCATGCTGGTTGGTAATAAGAATGACGCCAATGATGCGGTTGCCATCGGAGAAACGGCTGGCCGCCCTAAAGTGAGTCTA
CTTCGCCCTAAAACGGTCGAGCAGCAGGATATTCAATCTTTACTGCGCATTCGTGAGCGCTTGGTGGAGAATCGCACGGCTAACTGCAATCAACTGCGTG
GTTTTCTCGCGGAATACGGCCTGATTGTCGCAAAGACCCGCCGCCAGTTGATGTTAGCCATTCCGCTCATTCTTGAAGATGCTGACAACGAATTATCCAC
GGTTGCCAGAGGGTTTATTCAACGACTCTATGAGTTTCAGTTGTATTTAAATGAACAAATCGACCAGGTTGCACAGGACATATTAGCCCTTGCCCAAGCC
ATGCCACAGTATCCATTATTGCTAACCATCCCCGGTGTCGGCCCTATCGTCGCATCCACATTATTAGCCAGTATTAATGAAGTTAATGATTTTAAAAATG
GCCGGCAATTGGCGGCATGGCTAGGGCTCACTCCTTCACAATACAGCAGTGGCACGACCAATCGGCTCGGCAAGATAACCAAGCGCGGAAATCAAACGCT
CCGGCGGTTACTTATCCATGGCGCAAGAACCGTGGTGAACTGGTGCGATAAGAAAGACGACTCACTTAGCCTGTGGTTACAACGGCTTAAACAGCGTCAA
CACGTCTGCAAAGTGACGGTTGCCTTGGCCAATAAGATGGCTCGGATGGTGTTTGTAGTGATGAGCCGCGGACAAGCATTTGATATGAATAAGGCCTGTA
AATTGGCAACGTAATGCAGTGTTTTTTGCGTAAATAGACTCAAAATGTAAATAGATAAATGTCCATCAAAGCACAACAGCGGTAATGGTATTAATGAGTT
AAACGGTTTGTCTTACCCAAAACCTGATGAGGACAGTGGCCTTTGAGGCCATGTGGGTGCCAAGGAGGGTAAGACGTCAATTTGGATCTCATTAGGGCGC
AACAGACGCCGGATATATGTCGCAAGACCGCAAACCATTAACGACCGATACCCCGTTGTGAAAGGTGAATTATCGATTAGCACAAATTAATTTGAACAAA
ATAGGACATTGACCTTGCTAACCCCGAGGTGACCATATA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1035 bp | 344 aa | 80 | 1114 | + | No |
Chemistry : DEDD
ORF sequence :
MNYKLISIDLAKTVFQVAAFNPDNTIAFNRKINRPALLDMLRQCEPTLVVMEACYSANYWGRTIEKLGHTVKLIPARMVKAMLVGNKNDANDAVAIGETA
GRPKVSLLRPKTVEQQDIQSLLRIRERLVENRTANCNQLRGFLAEYGLIVAKTRRQLMLAIPLILEDADNELSTVARGFIQRLYEFQLYLNEQIDQVAQD
ILALAQAMPQYPLLLTIPGVGPIVASTLLASINEVNDFKNGRQLAAWLGLTPSQYSSGTTNRLGKITKRGNQTLRRLLIHGARTVVNWCDKKDDSLSLWL
QRLKQRQHVCKVTVALANKMARMVFVVMSRGQAFDMNKACKLAT
GRPKVSLLRPKTVEQQDIQSLLRIRERLVENRTANCNQLRGFLAEYGLIVAKTRRQLMLAIPLILEDADNELSTVARGFIQRLYEFQLYLNEQIDQVAQD
ILALAQAMPQYPLLLTIPGVGPIVASTLLASINEVNDFKNGRQLAAWLGLTPSQYSSGTTNRLGKITKRGNQTLRRLLIHGARTVVNWCDKKDDSLSLWL
QRLKQRQHVCKVTVALANKMARMVFVVMSRGQAFDMNKACKLAT
Blast result :
Comments
No uninterrupted target sequence was found, so the ends of the IS have been defined by analogy with other IS1111 family elements, assuming that 7 nt separate IRl from the left-hand end of the element and 4 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
There are 2 copies of ISSo14 in the Shewanella oneidensis MR-1 genome, with the transposase gene corresponding to orfs SO3945 and SO4548.
The transposase protein is 38% identical to that of IS1111.
There are 2 copies of ISSo14 in the Shewanella oneidensis MR-1 genome, with the transposase gene corresponding to orfs SO3945 and SO4548.
The transposase protein is 38% identical to that of IS1111.
References
1] Heidelberg et al. (2002) Nature Biotechnol. 20, 1118-1123
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384