ISSod2
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004347 | ND | Shewanella oneidensis | Shewanella oneidensis MR-1 |
DNA section
IS Length : 1186 bp
Ends
IR Length : 30/41
IRL : GGTAATCCCCCCTCAAATTAACTGACGTCAAAAGTAGAATTTTCTCGTAA
IRR : GGTAATCCTCCCATTTTTAACCGAAGTTAAAAGTAGAGTTCATGCAACCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGTAATCCCCCCTCAAATTAACTGACGTCAAAAGTAGAATTTTCTCGTAACATAAACGCAGGAGATTCACTATGAAAACATCAAAATTCACCGATAGCCA
AATCATGGCAATCCTTAAACAAGCTGAAGCCGGAACTCCAGTCCCAGAATTGTGCCGCGAACATGGCATGAGCTCAGCGACTTTTTACAAATGGCGAGCC
AAATTTGGCGGCATGGATGCGTCATTGATGGCTCGTCTAAAAGAGCTTGAAGCTGAAAATGCTCGACTGAAAAAGATGTATGCCGAAGAGCGGCTAAAGG
CCGATGTCATTCAGGAAGCCATGGCAAAAAAGTGGTAAAGCTGTCGCAACGTAAAGCGATGGCTCAGCATGCAGTTGCAAATCGAAAAATCAGTATCCGT
TTGGCCTGTGCAGCATTTTGTATCAGCGAAACCTGTTATCGCTACCGGCGCGTTAATGATGACGAAAATGCAGAGATTGCAGAATTTCTTGTTGAATTAA
CTGAAAAAGAATCGGATTGGGGCTTTGGTCAGTGTTTTAATTACTTACGCAACGTAAAAGGCTTTGCTTGGAACCACAAGCGTGTATACCGAATTTACTG
CGAATTAGCGTTAAATCTGCGGATAAAACCGCGCAGAAGGTTAAAACGAAATGCACCAGAGCCGCTGAAAGAGCCAACTAAAGCCAATCAGGTTTGGTCG
ATAGATTTTATGCATGACCAGTTATCAGATGGACGAAGCTATCGACTGTTCAACGTGATTGATGACTTTAAACGTGAAGGGCTCGCGATTGAAGCAGGTT
TTTCATTGCCAACAATGCGGGTGATCCGGACCCTAAATCAGCTACTTGAAGGGCGAACAAAACCAATCGCGATCCGTTGTGACAATGGACCTGAATTTAT
TAGCCACGAATTTACGGAATGGGCGAAAAAGCATGAGATCAGGATTGATTACATTCAACCTGGCAAGCCACAGCAAAATGCGTACATAGAACGACACAAT
AAGACCATCAGGTACAGCTGGGTAAGCAAACATTTATTTGATACGCTTGAAGAAGTTCAGGACTATGCCACAAAATGGTTATGGTTTTACAATCATGAAC
GGCCGCACAAAGCCAATGGCGGAAAGCCGCCATTGATGGTTGCATGAACTCTACTTTTAACTTCGGTTAAAAATGGGAGGATTACC
AATCATGGCAATCCTTAAACAAGCTGAAGCCGGAACTCCAGTCCCAGAATTGTGCCGCGAACATGGCATGAGCTCAGCGACTTTTTACAAATGGCGAGCC
AAATTTGGCGGCATGGATGCGTCATTGATGGCTCGTCTAAAAGAGCTTGAAGCTGAAAATGCTCGACTGAAAAAGATGTATGCCGAAGAGCGGCTAAAGG
CCGATGTCATTCAGGAAGCCATGGCAAAAAAGTGGTAAAGCTGTCGCAACGTAAAGCGATGGCTCAGCATGCAGTTGCAAATCGAAAAATCAGTATCCGT
TTGGCCTGTGCAGCATTTTGTATCAGCGAAACCTGTTATCGCTACCGGCGCGTTAATGATGACGAAAATGCAGAGATTGCAGAATTTCTTGTTGAATTAA
CTGAAAAAGAATCGGATTGGGGCTTTGGTCAGTGTTTTAATTACTTACGCAACGTAAAAGGCTTTGCTTGGAACCACAAGCGTGTATACCGAATTTACTG
CGAATTAGCGTTAAATCTGCGGATAAAACCGCGCAGAAGGTTAAAACGAAATGCACCAGAGCCGCTGAAAGAGCCAACTAAAGCCAATCAGGTTTGGTCG
ATAGATTTTATGCATGACCAGTTATCAGATGGACGAAGCTATCGACTGTTCAACGTGATTGATGACTTTAAACGTGAAGGGCTCGCGATTGAAGCAGGTT
TTTCATTGCCAACAATGCGGGTGATCCGGACCCTAAATCAGCTACTTGAAGGGCGAACAAAACCAATCGCGATCCGTTGTGACAATGGACCTGAATTTAT
TAGCCACGAATTTACGGAATGGGCGAAAAAGCATGAGATCAGGATTGATTACATTCAACCTGGCAAGCCACAGCAAAATGCGTACATAGAACGACACAAT
AAGACCATCAGGTACAGCTGGGTAAGCAAACATTTATTTGATACGCTTGAAGAAGTTCAGGACTATGCCACAAAATGGTTATGGTTTTACAATCATGAAC
GGCCGCACAAAGCCAATGGCGGAAAGCCGCCATTGATGGTTGCATGAACTCTACTTTTAACTTCGGTTAAAAATGGGAGGATTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 72 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSKFTDSQIMAILKQAEAGTPVPELCREHGMSSATFYKWRAKFGGMDASLMARLKELEAENARLKKMYAEERLKADVIQEAMAKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 891 bp | 296 aa | 257 | 1147 | + | No |
Description : Second part of the transposase
ORF sequence :
KCSTEKDVCRRAAKGRCHSGSHGKKVVKLSQRKAMAQHAVANRKISIRLACAAFCISETCYRYRRVNDDENAEIAEFLVELTEKESDWGFGQCFNYLRNV
KGFAWNHKRVYRIYCELALNLRIKPRRRLKRNAPEPLKEPTKANQVWSIDFMHDQLSDGRSYRLFNVIDDFKREGLAIEAGFSLPTMRVIRTLNQLLEGR
TKPIAIRCDNGPEFISHEFTEWAKKHEIRIDYIQPGKPQQNAYIERHNKTIRYSWVSKHLFDTLEEVQDYATKWLWFYNHERPHKANGGKPPLMVA
KGFAWNHKRVYRIYCELALNLRIKPRRRLKRNAPEPLKEPTKANQVWSIDFMHDQLSDGRSYRLFNVIDDFKREGLAIEAGFSLPTMRVIRTLNQLLEGR
TKPIAIRCDNGPEFISHEFTEWAKKHEIRIDYIQPGKPQQNAYIERHNKTIRYSWVSKHLFDTLEEVQDYATKWLWFYNHERPHKANGGKPPLMVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1076 bp | 358 aa | 72 | 1147 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSKFTDSQIMAILKQAEAGTPVPELCREHGMSSATFYKWRAKFGGMDASLMARLKELEAENARLKKMYAEERLKADVIQEAMAKKVVKLSQRKAMAQH
AVANRKISIRLACAAFCISETCYRYRRVNDDENAEIAEFLVELTEKESDWGFGQCFNYLRNVKGFAWNHKRVYRIYCELALNLRIKPRRRLKRNAPEPLK
EPTKANQVWSIDFMHDQLSDGRSYRLFNVIDDFKREGLAIEAGFSLPTMRVIRTLNQLLEGRTKPIAIRCDNGPEFISHEFTEWAKKHEIRIDYIQPGKP
QQNAYIERHNKTIRYSWVSKHLFDTLEEVQDYATKWLWFYNHERPHKANGGKPPLMVA
AVANRKISIRLACAAFCISETCYRYRRVNDDENAEIAEFLVELTEKESDWGFGQCFNYLRNVKGFAWNHKRVYRIYCELALNLRIKPRRRLKRNAPEPLK
EPTKANQVWSIDFMHDQLSDGRSYRLFNVIDDFKREGLAIEAGFSLPTMRVIRTLNQLLEGRTKPIAIRCDNGPEFISHEFTEWAKKHEIRIDYIQPGKP
QQNAYIERHNKTIRYSWVSKHLFDTLEEVQDYATKWLWFYNHERPHKANGGKPPLMVA
Blast result :
Comments
There are 5 copies of ISSod2 in the chromosome and 0 copies in the plasmid of this strain.
ISSod2 is 89% aa similar to ISAca1.
ISSod2 is 89% aa similar to ISAca1.
References
1] Bordi, C., M. Ansaldi, S. Gon, C. Jourlin-Castelli, C. Iobbi-Nivol, and V. Mejean. (2004) J. Bacteriol.186(14):4502-4509