ISDha1
- Family IS256
- Group
Isoform Synonym(s) ISDesp1, ISDesp2, ISDesp3
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF439608 | ND | Desulfitobacterium hafniense | Desulfitobacterium sp. Y51 Desulfitobacterium hafniense TCE1 |
DNA section
IS Length : 1570 bp
Ends
IR Length : 26
IRL : GATAGAGTACAATTAAGTTCGCAAAAGTAAAAAAGGAAAAGCCATCGGTA
IRR : GATAGTGTCAAGAAATGTTCGCAAAAACCATCAGAACCCCTTAAGGCTAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGTTTAGAT | TTTTTATA | GGGTGTGTTT | 8 |
DNA sequence
GATAGAGTACAATTAAGTTCGCAAAAGTAAAAAAGGAAAAGCCATCGGTAACTCCGAAAAGGTAAGTGCTGAAGCAAACCAAAAAGGAGTTGTTAACCGA
TGGCTTACCCAAATTCTACCGTACCTTTTGAAAAAATGCTAATGAAATTTGTAACCGAAGAAGATCCCATGCAGGCTATGTTAAAATGGCTCTGCGAAAG
GCTGATGGAAGCAGAAGTGGATGCAAAGCTAGGTGCTGAAAAATCAGAACGGTCTACTGAACGTCAAGGGTATCGATCAGGCTATCGCGTTCGCCGTTTC
GATACCCGGATGGGGACACTGTATTTAATGGTACCCAAACTGCGAAATGGCGGCTATATCCCTTTCTTTGTGGAAGGTAAGAAACGTTCTGAATTGGCCT
TGATGAATGTGATTCAAGAAGCCTATGTTAATGGCGTATCCACCCGTAAAATAGAGAAACTCACTAAGTCCTTAGGCATTGACTCCATGTCACGAAGTCA
GGTATCAAGCATCGCTAAAGAACTGAACGAACAGGTTGAAGCCTTTCGTAATCGAAGTCTCGATAAAGTCTATCCCGTACTCTGGGTGGATGCTCTCTAT
GAGAAAATCCGAGATGATCGAAGGGTCAAAAATATGGCAGTGCTGATTGTAACGGGTATTGATCTAGAAGGCAAACGAGATATTCTGGCCGTAGAGCCCA
TGCCAGAAGAGTCCACAGCAACCTACACCAGTCTCTTCGAAAAGTTAAAGTCACGAGGACTAGAGAAAGTCTGGCTGGTTGTCTCAGATGCCCATAAAGG
GTTAGTTAAAGCTGTTCAAGAGTCCTTTATCGGGTGTTCCTGGCAACGCTGTAAAGTGCATTTTATGCGGAACATCCTCGCTCATATATCCGGTCGAGAT
AAGCAAGCGTTTGCTAATAAACTAAAGCAAATCTGGCTTCAACCAGACTATAGCAGTGCAAAGAAATACGCTAATTCTCTGATGGATGAGTTTGAGGCCA
AATATCCTGAAGCCATTAAAACTCTGGAAGAAGGGTTGGAAGACTCACTACAGTTTTTCAGCTTTGCAGAGATTGATTCAAGAAAGATCGCGTCAACCAA
CCTGCTGGAAAGACTCAACCGAGAAGTACGTAGAAGAACCCGGGTGGTTGGGATATTCCCAAGTATGGATTCCTATATTCGGCTAGTAACCAGCTACCTT
ATTGAGTATAGCGAAGACTGGTGCAGTGGCCGATCCTATATCAATCCAAAAATCATCACTTCGATTGAGCAGGAACGTTCAAAAGCTGCGTAAGCCTTAA
TCATTAATTCAGAAAAGCATGACGCTCGGCGCTAGCCAAGCAAACTCTCTTTGATTTAGGGTTACTCTTTGGGGTGAAAGAGACTTTGCCGTCTTCAAGC
GTTTTGGTAGGGTGCACGTGTCAATGGGATGATGCCGAGCATATCCTCGGCCTGACGGCCTCCGGTATCCCTCGCATCACCCTTGACACTCTTCCCAACT
TACGCTTTTACATAAAACATTTAGCCTTAAGGGGTTCTGATGGTTTTTGCGAACATTTCTTGACACTATC
TGGCTTACCCAAATTCTACCGTACCTTTTGAAAAAATGCTAATGAAATTTGTAACCGAAGAAGATCCCATGCAGGCTATGTTAAAATGGCTCTGCGAAAG
GCTGATGGAAGCAGAAGTGGATGCAAAGCTAGGTGCTGAAAAATCAGAACGGTCTACTGAACGTCAAGGGTATCGATCAGGCTATCGCGTTCGCCGTTTC
GATACCCGGATGGGGACACTGTATTTAATGGTACCCAAACTGCGAAATGGCGGCTATATCCCTTTCTTTGTGGAAGGTAAGAAACGTTCTGAATTGGCCT
TGATGAATGTGATTCAAGAAGCCTATGTTAATGGCGTATCCACCCGTAAAATAGAGAAACTCACTAAGTCCTTAGGCATTGACTCCATGTCACGAAGTCA
GGTATCAAGCATCGCTAAAGAACTGAACGAACAGGTTGAAGCCTTTCGTAATCGAAGTCTCGATAAAGTCTATCCCGTACTCTGGGTGGATGCTCTCTAT
GAGAAAATCCGAGATGATCGAAGGGTCAAAAATATGGCAGTGCTGATTGTAACGGGTATTGATCTAGAAGGCAAACGAGATATTCTGGCCGTAGAGCCCA
TGCCAGAAGAGTCCACAGCAACCTACACCAGTCTCTTCGAAAAGTTAAAGTCACGAGGACTAGAGAAAGTCTGGCTGGTTGTCTCAGATGCCCATAAAGG
GTTAGTTAAAGCTGTTCAAGAGTCCTTTATCGGGTGTTCCTGGCAACGCTGTAAAGTGCATTTTATGCGGAACATCCTCGCTCATATATCCGGTCGAGAT
AAGCAAGCGTTTGCTAATAAACTAAAGCAAATCTGGCTTCAACCAGACTATAGCAGTGCAAAGAAATACGCTAATTCTCTGATGGATGAGTTTGAGGCCA
AATATCCTGAAGCCATTAAAACTCTGGAAGAAGGGTTGGAAGACTCACTACAGTTTTTCAGCTTTGCAGAGATTGATTCAAGAAAGATCGCGTCAACCAA
CCTGCTGGAAAGACTCAACCGAGAAGTACGTAGAAGAACCCGGGTGGTTGGGATATTCCCAAGTATGGATTCCTATATTCGGCTAGTAACCAGCTACCTT
ATTGAGTATAGCGAAGACTGGTGCAGTGGCCGATCCTATATCAATCCAAAAATCATCACTTCGATTGAGCAGGAACGTTCAAAAGCTGCGTAAGCCTTAA
TCATTAATTCAGAAAAGCATGACGCTCGGCGCTAGCCAAGCAAACTCTCTTTGATTTAGGGTTACTCTTTGGGGTGAAAGAGACTTTGCCGTCTTCAAGC
GTTTTGGTAGGGTGCACGTGTCAATGGGATGATGCCGAGCATATCCTCGGCCTGACGGCCTCCGGTATCCCTCGCATCACCCTTGACACTCTTCCCAACT
TACGCTTTTACATAAAACATTTAGCCTTAAGGGGTTCTGATGGTTTTTGCGAACATTTCTTGACACTATC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1194 bp | 1194 aa | 100 | 1293 | + | No |
Chemistry : DDE
ORF sequence :
MAYPNSTVPFEKMLMKFVTEEDPMQAMLKWLCERLMEAEVDAKLGAEKSERSTERQGYRSGYRVRRFDTRMGTLYLMVPKLRNGGYIPFFVEGKKRSELA
LMNVIQEAYVNGVSTRKIEKLTKSLGIDSMSRSQVSSIAKELNEQVEAFRNRSLDKVYPVLWVDALYEKIRDDRRVKNMAVLIVTGIDLEGKRDILAVEP
MPEESTATYTSLFEKLKSRGLEKVWLVVSDAHKGLVKAVQESFIGCSWQRCKVHFMRNILAHISGRDKQAFANKLKQIWLQPDYSSAKKYANSLMDEFEA
KYPEAIKTLEEGLEDSLQFFSFAEIDSRKIASTNLLERLNREVRRRTRVVGIFPSMDSYIRLVTSYLIEYSEDWCSGRSYINPKIITSIEQERSKAA
LMNVIQEAYVNGVSTRKIEKLTKSLGIDSMSRSQVSSIAKELNEQVEAFRNRSLDKVYPVLWVDALYEKIRDDRRVKNMAVLIVTGIDLEGKRDILAVEP
MPEESTATYTSLFEKLKSRGLEKVWLVVSDAHKGLVKAVQESFIGCSWQRCKVHFMRNILAHISGRDKQAFANKLKQIWLQPDYSSAKKYANSLMDEFEA
KYPEAIKTLEEGLEDSLQFFSFAEIDSRKIASTNLLERLNREVRRRTRVVGIFPSMDSYIRLVTSYLIEYSEDWCSGRSYINPKIITSIEQERSKAA
Blast result :
Comments
Desulfitobacterium hafniense strain TCE1 is known of dechlorinate tetrachloroethene
part of a composite transposon (Tn-Dha1), containing tetrachloroethene reductive dehalogenase genes.
DR present not around single ISDha1 copy, but around composite transposon.
indication of circular intermediates of transposition
Desulfitobacterium sp. strain Y51 dechlorinates tetrachloroethene to cis-1,2-dichloroethene by the PceA reductive dehalogenase. The pceA gene is located between ISDesp1 and ISDesp2 and they form a composite transposon. This structure is very similar to Tn-Dha1 in Desulfitobacterium hafniense strain TCE1 described by Maillard et al.
part of a composite transposon (Tn-Dha1), containing tetrachloroethene reductive dehalogenase genes.
DR present not around single ISDha1 copy, but around composite transposon.
indication of circular intermediates of transposition
Desulfitobacterium sp. strain Y51 dechlorinates tetrachloroethene to cis-1,2-dichloroethene by the PceA reductive dehalogenase. The pceA gene is located between ISDesp1 and ISDesp2 and they form a composite transposon. This structure is very similar to Tn-Dha1 in Desulfitobacterium hafniense strain TCE1 described by Maillard et al.
References
1] Maillard J, Regeard C, Holliger C. (2005) Environ Microbiol. 7(1):107-17.
2] Futagami T, Tsuboi Y, Suyama A, Goto M, Furukawa K. (2006) Appl Microbiol Biotechnol.70(6):720-8
2] Futagami T, Tsuboi Y, Suyama A, Goto M, Furukawa K. (2006) Appl Microbiol Biotechnol.70(6):720-8