ISBlo11
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004307 | ND | Bifidobacterium longum | Bifidobacterium longum NCC2705 Bifidobacterium longum subsp. longum JDM301 Bifidobacterium longum subsp. infantis ATCC 15697 Bifidobacterium longum subsp. infantis JCM 1222 |
DNA section
IS Length : 1432 bp
Ends
IR Length : 26/38
IRL : GGGGATGCGGACGGAAAGTTGGACGCTTGGGCGTCCGGATTGGAGTCCGC
IRR : GGGGGTGCGGATGTTTTTTTGGACGGTCAGGCGGCCAGTCCAAGACTTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGTATAGAAT | CTC | ACTAGAAAAT | 3 |
| AAATCGGGCG | TTA | TCGGATATGC | 3 |
| ATTTCCCTCA | TCC | CAAACTATGA | 3 |
| GTCGAATTCC | GAA | CGCCCGAATT | 3 |
| GTTGTCTAAG | TAC | TATGGGAAAG | 3 |
| GGAGAAGCCG | GCA | TTCACAAAAT | 3 |
| TTTACTTTCG | GTG | ATAAAACTTG | 3 |
DNA sequence
GGGGATGCGGACGGAAAGTTGGACGCTTGGGCGTCCGGATTGGAGTCCGCATGTTTTCGATGGAGGATCGGCGCAGGGCCGTTGACCTGTATTTCACGGA
GGGCATGACCATCAGGAAGGTCATTGCCGAGCTTGGTTACCCGTCCGAGGGCGCGCTGGTGAAATGGGTTCGCGAGGACCCGCGTTACACGGGCGCGTGC
AGGCGTTCGTATACGCTGGAATGCAAGACGAATGCGGCCAGACGCGCGCTCGAGGGCGAGCCCCTCGCCCGCGTGGCGCGCGACGCGGGTTGCACGCCGA
CGAGCGTGTACCAGTGGATGCGCCGCTACCGGAGCGAGGGGATACTGGGACTGATGGACAGGAGAAACGCGCCGATGCCGGCCGAACCGATGCCCGCGGC
CGGCGATGACGTGGAGGAGCTGCGCCGGCAGGTGGAGGTCCTGCGGCTGGAGAACGCGGTGATGCGGGAGACGATCGATGTTTTAAAAGCCGACGACCCG
CGCCTCGACCCGTCCATGCTGACGAACAGGGAGAGGACGCGGGTCGTCGACGCGATCAGGGGTGAATTCGGCCTGGCGGCCTGTCTGAAGGCCGTGGGGC
TGAAACGCAGCACCTACTATTACGAGCGCGGCGCGATCGCCGCGGGCGACAGGTACGCCGTGCTGCGGGCCCGCGTCGCCGGCCTGTTCGAGCAGGGTGG
GCGCGCATGGGGATACCGGACCATCCACCGCATGCTGCGCCTTGACGAGTCCGACCCGATCGTCGTCTCGGAGAAGGTCGTCAGGCGGATCATGCGCGAG
GGGGCCATGCGGCCCGTGTACCTGAAGCGGCCGAAGCGGTGGAGCTCCTACGCCGGCGAGATCGGCGAGGCCCCGGCGAACCTCGTGGAGCGGGACTTCC
ACGCCGACGCGCCGAACATGCTGTGGGTCACGGACGTCACCCAGTTCACCATGGACGGGTACAAGTGCTGGCTTTCCCCGGTGGTCGACTGCTTCGACGG
GATGGTGGTCTCCTGGACCTTGTCGCGCTCCCCGAACGCCGACATGGCGAACCGGATGCTCCTTGACGCGGTCGCCACGCTCAGGGACGGGGAACATCCA
ATCATCCATTCCGACCGCGGCTGCCACTACCGGTGGGACGAATGGATCCGCATCTGCGAGGAGCACGGGCTGATCCGGTCGATGAGCGCCAAGGGATGCA
GCCCGGACAACGCGGCCGCCGAGGGGTTCTTCGGCAGGCTCAAGAACGAGTTATTCTACGGGCGGGACTGGAGGGGCGTGGGCTACGAGGAGTTCCGCGA
ACGCCTGGCCGCCTACCTGACCCACTACAATGAAACCAGGATCAAGAAGTCACTGGACTGGATGAGCCCCGTGCAATACCGCAGAAGTCTTGGACTGGCC
GCCTGACCGTCCAAAAAAACATCCGCACCCCC
GGGCATGACCATCAGGAAGGTCATTGCCGAGCTTGGTTACCCGTCCGAGGGCGCGCTGGTGAAATGGGTTCGCGAGGACCCGCGTTACACGGGCGCGTGC
AGGCGTTCGTATACGCTGGAATGCAAGACGAATGCGGCCAGACGCGCGCTCGAGGGCGAGCCCCTCGCCCGCGTGGCGCGCGACGCGGGTTGCACGCCGA
CGAGCGTGTACCAGTGGATGCGCCGCTACCGGAGCGAGGGGATACTGGGACTGATGGACAGGAGAAACGCGCCGATGCCGGCCGAACCGATGCCCGCGGC
CGGCGATGACGTGGAGGAGCTGCGCCGGCAGGTGGAGGTCCTGCGGCTGGAGAACGCGGTGATGCGGGAGACGATCGATGTTTTAAAAGCCGACGACCCG
CGCCTCGACCCGTCCATGCTGACGAACAGGGAGAGGACGCGGGTCGTCGACGCGATCAGGGGTGAATTCGGCCTGGCGGCCTGTCTGAAGGCCGTGGGGC
TGAAACGCAGCACCTACTATTACGAGCGCGGCGCGATCGCCGCGGGCGACAGGTACGCCGTGCTGCGGGCCCGCGTCGCCGGCCTGTTCGAGCAGGGTGG
GCGCGCATGGGGATACCGGACCATCCACCGCATGCTGCGCCTTGACGAGTCCGACCCGATCGTCGTCTCGGAGAAGGTCGTCAGGCGGATCATGCGCGAG
GGGGCCATGCGGCCCGTGTACCTGAAGCGGCCGAAGCGGTGGAGCTCCTACGCCGGCGAGATCGGCGAGGCCCCGGCGAACCTCGTGGAGCGGGACTTCC
ACGCCGACGCGCCGAACATGCTGTGGGTCACGGACGTCACCCAGTTCACCATGGACGGGTACAAGTGCTGGCTTTCCCCGGTGGTCGACTGCTTCGACGG
GATGGTGGTCTCCTGGACCTTGTCGCGCTCCCCGAACGCCGACATGGCGAACCGGATGCTCCTTGACGCGGTCGCCACGCTCAGGGACGGGGAACATCCA
ATCATCCATTCCGACCGCGGCTGCCACTACCGGTGGGACGAATGGATCCGCATCTGCGAGGAGCACGGGCTGATCCGGTCGATGAGCGCCAAGGGATGCA
GCCCGGACAACGCGGCCGCCGAGGGGTTCTTCGGCAGGCTCAAGAACGAGTTATTCTACGGGCGGGACTGGAGGGGCGTGGGCTACGAGGAGTTCCGCGA
ACGCCTGGCCGCCTACCTGACCCACTACAATGAAACCAGGATCAAGAAGTCACTGGACTGGATGAGCCCCGTGCAATACCGCAGAAGTCTTGGACTGGCC
GCCTGACCGTCCAAAAAAACATCCGCACCCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1356 bp | 451 aa | 51 | 1406 | + | No |
Chemistry : DDE
ORF sequence :
MFSMEDRRRAVDLYFTEGMTIRKVIAELGYPSEGALVKWVREDPRYTGACRRSYTLECKTNAARRALEGEPLARVARDAGCTPTSVYQWMRRYRSEGILG
LMDRRNAPMPAEPMPAAGDDVEELRRQVEVLRLENAVMRETIDVLKADDPRLDPSMLTNRERTRVVDAIRGEFGLAACLKAVGLKRSTYYYERGAIAAGD
RYAVLRARVAGLFEQGGRAWGYRTIHRMLRLDESDPIVVSEKVVRRIMREGAMRPVYLKRPKRWSSYAGEIGEAPANLVERDFHADAPNMLWVTDVTQFT
MDGYKCWLSPVVDCFDGMVVSWTLSRSPNADMANRMLLDAVATLRDGEHPIIHSDRGCHYRWDEWIRICEEHGLIRSMSAKGCSPDNAAAEGFFGRLKNE
LFYGRDWRGVGYEEFRERLAAYLTHYNETRIKKSLDWMSPVQYRRSLGLAA
LMDRRNAPMPAEPMPAAGDDVEELRRQVEVLRLENAVMRETIDVLKADDPRLDPSMLTNRERTRVVDAIRGEFGLAACLKAVGLKRSTYYYERGAIAAGD
RYAVLRARVAGLFEQGGRAWGYRTIHRMLRLDESDPIVVSEKVVRRIMREGAMRPVYLKRPKRWSSYAGEIGEAPANLVERDFHADAPNMLWVTDVTQFT
MDGYKCWLSPVVDCFDGMVVSWTLSRSPNADMANRMLLDAVATLRDGEHPIIHSDRGCHYRWDEWIRICEEHGLIRSMSAKGCSPDNAAAEGFFGRLKNE
LFYGRDWRGVGYEEFRERLAAYLTHYNETRIKKSLDWMSPVQYRRSLGLAA
Blast result :
Comments
Like IS1221A and ISL6, ISBlo11 seems to do without a programmed translational frameshift to produce the full-length transposase.
ISBlo11 is 59% aa similar to ISBmu11.
ISBlo11 is 59% aa similar to ISBmu11.
References
1] Schell,M.A., Karmirantzou,M., Snel,B., Vilanova,D., Berger,B., Pessi,G., Zwahlen,M.-C., Desiere,F., Bork,P., Delley,M., Pridmore,D. and Arigoni,F. (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (22), 14422-14427