ISBlo5
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004307 | ND | Bifidobacterium longum | Bifidobacterium longum NCC2705 |
DNA section
IS Length : 1366 bp
Ends
IR Length : 24/32
IRL : GATAACGCCCGATTTTTTGCGCACTTTCGGATTCCGGGTCTGACGTAAGA
IRR : GGTAGTGCCCGAAAAGTTGCGCACTTTGGATCGTGGCCGTCCATGGCGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| tcttcgaggt | gaagcaga | tgccttcttc | 8 |
| agacgccggc | gcgcgcgg | tcgatgacgg | 8 |
| tattgttgtc | gctattgt | tggactcgtc | 8 |
| taccggtgct | caatatga | tgattctcat | 8 |
DNA sequence
GATAACGCCCGATTTTTTGCGCACTTTCGGATTCCGGGTCTGACGTAAGAGGGCATGGCCCGCGTCCTGTGTACGATTTTCATTCGCCAAAACAAAAAAC
CGCGATTGGAAGCCAGGAGACGGAACCATGCCCGAGAACATCATACAGATCGACCAGAACCTGCTCGAGACCAGGCTCGACCGCTTGGTCGCCGAAAAGG
TCGCCGAGCTATTGAACGCGATGCTCGACGCCGAGGCCGACGAGATCGCCAACGCCGCCAAGTACGAGCGCACCGGGGAGCGGAAGGCGTACAGGGCCGG
CCACTACGACCGGAGCCTGACCGCCAAGGCCGGCAGGCTCGCGTTGAAGGTGCCCAAACTCAAGGGCGCGGTCTTCGAGTCCGCGGTCATCGAACGATAC
CGGAGACGCGAGGAGAGTGTCGAGGAGGCCCTCATCGACATGTACCTGGCCGGCGTGAGCACCCGGCAGGTGGACGACATCAGCCAATTGCTGTGGGGCG
ACAGGATGCCGTCCCAGACCCTGAGCGACAAGCTCAAGAAGGTGTACGGGGAGATCGAGGCGTGGCGCACGAGGCCGTTGGAGGACGAGTACCCGTACGT
GTTCATGGACGGCGTGTGGCACAAGCGTTCATGGGGCGGGCACGTGGAGAACGTCAGCGTCCTGGTCGCCATCGGAGTCAACGCGGAGGGGCACCGCGAG
GTGATCGGCGTGGCCGAGGGCATGAAGGAGGACAGGGACAGCTGGGAGCAGTTCGTCCGCTCCATGATCGAACGCGGCCTGAAGGGCGTGCGCCTGGTGG
TCGGCGACCGGTGCGCCGGACTCGTCGCCACGGTCGGCTCGATGCTGCCCAAGGCGAGATACCAGCGGTGCATGGTGCATTTCATGCGCAACGTGCTCTC
GAAGACGCCGCCGACACACCGCGAATGGGCGTCCGCCGCCCTCAAGGCCATATTCGCCATGGAATCCCGGGAATCCGCCCTGGCCAAGGCCGAACAGGTC
GCCACGGAGATGGAATCCAGGAGGCTCAAGGCCGCCGCGAACTGCCTGCGCGAGGGCGTCGGCGAGACGACGACCTACCTGCTGGACGAGTTCCCGGACG
GGCACCGCAGGAGGATCCGCACGAACAACATGATCGAGCGGCTGAACAGGGAGATCCGCCGGCGCACGCGCGTGGTGGGCGGCTTCCCCGACGGGAACAG
CGCGTTGATGCTCATATGCGCCCGCATCCGTCACGTCACCGCGAACGAATGGTCGACCCGCCGCTATCTCGACATGTCCCGGCTCGGTGACAACCTCGTG
GAAGCGAACTGACTATCGCGCCATGGACGGCCACGATCCAAAGTGCGCAACTTTTCGGGCACTACC
CGCGATTGGAAGCCAGGAGACGGAACCATGCCCGAGAACATCATACAGATCGACCAGAACCTGCTCGAGACCAGGCTCGACCGCTTGGTCGCCGAAAAGG
TCGCCGAGCTATTGAACGCGATGCTCGACGCCGAGGCCGACGAGATCGCCAACGCCGCCAAGTACGAGCGCACCGGGGAGCGGAAGGCGTACAGGGCCGG
CCACTACGACCGGAGCCTGACCGCCAAGGCCGGCAGGCTCGCGTTGAAGGTGCCCAAACTCAAGGGCGCGGTCTTCGAGTCCGCGGTCATCGAACGATAC
CGGAGACGCGAGGAGAGTGTCGAGGAGGCCCTCATCGACATGTACCTGGCCGGCGTGAGCACCCGGCAGGTGGACGACATCAGCCAATTGCTGTGGGGCG
ACAGGATGCCGTCCCAGACCCTGAGCGACAAGCTCAAGAAGGTGTACGGGGAGATCGAGGCGTGGCGCACGAGGCCGTTGGAGGACGAGTACCCGTACGT
GTTCATGGACGGCGTGTGGCACAAGCGTTCATGGGGCGGGCACGTGGAGAACGTCAGCGTCCTGGTCGCCATCGGAGTCAACGCGGAGGGGCACCGCGAG
GTGATCGGCGTGGCCGAGGGCATGAAGGAGGACAGGGACAGCTGGGAGCAGTTCGTCCGCTCCATGATCGAACGCGGCCTGAAGGGCGTGCGCCTGGTGG
TCGGCGACCGGTGCGCCGGACTCGTCGCCACGGTCGGCTCGATGCTGCCCAAGGCGAGATACCAGCGGTGCATGGTGCATTTCATGCGCAACGTGCTCTC
GAAGACGCCGCCGACACACCGCGAATGGGCGTCCGCCGCCCTCAAGGCCATATTCGCCATGGAATCCCGGGAATCCGCCCTGGCCAAGGCCGAACAGGTC
GCCACGGAGATGGAATCCAGGAGGCTCAAGGCCGCCGCGAACTGCCTGCGCGAGGGCGTCGGCGAGACGACGACCTACCTGCTGGACGAGTTCCCGGACG
GGCACCGCAGGAGGATCCGCACGAACAACATGATCGAGCGGCTGAACAGGGAGATCCGCCGGCGCACGCGCGTGGTGGGCGGCTTCCCCGACGGGAACAG
CGCGTTGATGCTCATATGCGCCCGCATCCGTCACGTCACCGCGAACGAATGGTCGACCCGCCGCTATCTCGACATGTCCCGGCTCGGTGACAACCTCGTG
GAAGCGAACTGACTATCGCGCCATGGACGGCCACGATCCAAAGTGCGCAACTTTTCGGGCACTACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1185 bp | 394 aa | 128 | 1312 | + | No |
Chemistry : DDE
ORF sequence :
MPENIIQIDQNLLETRLDRLVAEKVAELLNAMLDAEADEIANAAKYERTGERKAYRAGHYDRSLTAKAGRLALKVPKLKGAVFESAVIERYRRREESVEE
ALIDMYLAGVSTRQVDDISQLLWGDRMPSQTLSDKLKKVYGEIEAWRTRPLEDEYPYVFMDGVWHKRSWGGHVENVSVLVAIGVNAEGHREVIGVAEGMK
EDRDSWEQFVRSMIERGLKGVRLVVGDRCAGLVATVGSMLPKARYQRCMVHFMRNVLSKTPPTHREWASAALKAIFAMESRESALAKAEQVATEMESRRL
KAAANCLREGVGETTTYLLDEFPDGHRRRIRTNNMIERLNREIRRRTRVVGGFPDGNSALMLICARIRHVTANEWSTRRYLDMSRLGDNLVEAN
ALIDMYLAGVSTRQVDDISQLLWGDRMPSQTLSDKLKKVYGEIEAWRTRPLEDEYPYVFMDGVWHKRSWGGHVENVSVLVAIGVNAEGHREVIGVAEGMK
EDRDSWEQFVRSMIERGLKGVRLVVGDRCAGLVATVGSMLPKARYQRCMVHFMRNVLSKTPPTHREWASAALKAIFAMESRESALAKAEQVATEMESRRL
KAAANCLREGVGETTTYLLDEFPDGHRRRIRTNNMIERLNREIRRRTRVVGGFPDGNSALMLICARIRHVTANEWSTRRYLDMSRLGDNLVEAN
Blast result :
Comments
The genome of NCC2705 shows four complete copy of ISBlo5 as well as two partial altered (about 77% identity) non-overlapping pieces. These latter might be the remains of one copy.
Coordinates:
ISBlo5a 593168..594533
ISBlo5b complement(1021197..1022562)
ISBlo5c 853915..855280
ISBlo5d 1886182..1887547
ISBlo5e 284955..285395
ISBlo5f 676382..676620
DR :
The four complete copies show a 8 bp target duplication, but the DR of ISBlo5c and ISBlo5d are cross-exchanged. This bears witness to a large recombination event (about 1 Gbp between the two elements). This can be linked to two disruptions of the GC-skew along the chromosome.
Identity between IS copies:
ISBlo5a 100%
ISBlo5b 100%
ISBlo5c 100%
ISBlo5d 100%
ISBlo5e 78.9%
ISBlo5f 75.0%
Coordinates:
ISBlo5a 593168..594533
ISBlo5b complement(1021197..1022562)
ISBlo5c 853915..855280
ISBlo5d 1886182..1887547
ISBlo5e 284955..285395
ISBlo5f 676382..676620
DR :
The four complete copies show a 8 bp target duplication, but the DR of ISBlo5c and ISBlo5d are cross-exchanged. This bears witness to a large recombination event (about 1 Gbp between the two elements). This can be linked to two disruptions of the GC-skew along the chromosome.
Identity between IS copies:
ISBlo5a 100%
ISBlo5b 100%
ISBlo5c 100%
ISBlo5d 100%
ISBlo5e 78.9%
ISBlo5f 75.0%
References
1] Schell,M.A., Karmirantzou,M., Snel,B., Vilanova,D., Berger,B., Pessi,G., Zwahlen,M.-C., Desiere,F., Bork,P., Delley,M., Pridmore,D. and Arigoni,F. (2002)Proc. Natl. Acad. Sci. U.S.A. 99 (22), 14422-14427