ISPsy9
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AE016853 | ND | Pseudomonas syringae | Pseudomonas syringae DC3000 |
DNA section
IS Length : 1452 bp
Ends
IR Length : 23
IRL : TGGTGTGACCCCCGAAGGTTGGATGCAACCTTTGGAGGCGTCATGGGTAA
IRR : TGGTGTGACCCCCGAAGGTTGGACAATTATCCACCTACGCTGCCTTGGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTCCTCAC | CTC | CAGCGTGGGA | 3 |
DNA sequence
TGGTGTGACCCCCGAAGGTTGGATGCAACCTTTGGAGGCGTCATGGGTAAATACACAGAGCAGGCAAAACTCGCAGCCGTGAAAGAGTATTGTGCCGGCA
AGGCAGGTTTGAGGGATGTTGCGCATCGCCACGATGTGGACTTTTCCTGCCTTCGTCAGTGGGTCGCGGCTTATCAGATCCACGGCCCAGCCGCTCTTAA
AGAGAAAAGGCGGCAGCGTTACAGTGACGAATTCAAGCTGGCTGTTTTGAAGCGCATGCATGATGAGCGATTATCACTGCGCCAGACAGCGGCCTTGTTC
GATATCCGTCAGTTCGGCATCATCAATCTATGGCAGCGCCTTTTTGACGAAGGTGCCCTTAACGCATCCTCAAAGCCCCCTAAGAAAGCTGGACGCCCAA
GAAAGATGACGACGCCCCTTCCCCCTGTGAACTCCTCTCCGATCAACGATGAATCGCGCTCGCGTGACGAGTTGCTTGCCGAGGTGAAGCAACTGCGGAT
GGAGGTCGATTATCTAAAAAAGCTCGATGCCTTGGTTCAGAAGAAGCAACGAATAGCGCAGCAGAAAAAGCGCAAATCGTGATGGAACTGAGGCCTTTGC
ATTCTCTGGACGGCCTGCTGAAGTTTGCCGGTCTGGCACGCAGCACTTTTTACTATCAACAAAAGGTACTGCTGGCAGATGACAAATACGCCGGGCTGAA
AGACCTGATTCAGGCAACTTTCTACGAGCACAAGGGCCGGTATGGCTATCGTCGCATCACGGCAGCTTTGCGCAGGGCGGGCCATCTTGTGAACCACAAG
ACGGTGCAGAAACTAATGGGGCAGCTAGGCCTGAAGAGCCTGATACGCGTGAAGAAATACCGTTCCTACAAAGGCGAAACAGGCAAGGCCGCGCCTAACC
TGCTAAAGCGTGATTTCAAAGCGCAATACCTTAACCAGAAATGGGCCACCGACGTGACCGAATTCAAAGTAGGAGGCCAGAAACTCTATCTGTCGCCGAT
CATGGATCTGTACAGCGGCGAAATCATTTCCTATGCGATTGCCAGGCGCCCGCTGTACTCCATGGTCGACGAAATGCTTGAAGGAGCATTTAAGCGGCTT
GGACCGCATGAAAAGCCGATTTTGCATTCCGACCAGGGCTGGCAATATCGGATGCCTATTTACCAGCGACTGCTCAATGAGAATTCGATCACAGCCAGCA
TGTCGCGCAAGGGTAACTGCTACGACAACGCGGCTATTGAGAGTTTTTTTAGCACGCTGAAATTCGAATTTTTCTACCTGAACAAATTTAACGATCTGGA
TGAGCTTCAAGCGGGTATCGAAGAGTACATTGAGTATTACAATCACTCACGCATAAAACTGAAGCTTAACGGCCTGAGTCCTGTGGAATACAGAATGCAG
GCCGCCAAGGCAGCGTAGGTGGATAATTGTCCAACCTTCGGGGGTCACACCA
AGGCAGGTTTGAGGGATGTTGCGCATCGCCACGATGTGGACTTTTCCTGCCTTCGTCAGTGGGTCGCGGCTTATCAGATCCACGGCCCAGCCGCTCTTAA
AGAGAAAAGGCGGCAGCGTTACAGTGACGAATTCAAGCTGGCTGTTTTGAAGCGCATGCATGATGAGCGATTATCACTGCGCCAGACAGCGGCCTTGTTC
GATATCCGTCAGTTCGGCATCATCAATCTATGGCAGCGCCTTTTTGACGAAGGTGCCCTTAACGCATCCTCAAAGCCCCCTAAGAAAGCTGGACGCCCAA
GAAAGATGACGACGCCCCTTCCCCCTGTGAACTCCTCTCCGATCAACGATGAATCGCGCTCGCGTGACGAGTTGCTTGCCGAGGTGAAGCAACTGCGGAT
GGAGGTCGATTATCTAAAAAAGCTCGATGCCTTGGTTCAGAAGAAGCAACGAATAGCGCAGCAGAAAAAGCGCAAATCGTGATGGAACTGAGGCCTTTGC
ATTCTCTGGACGGCCTGCTGAAGTTTGCCGGTCTGGCACGCAGCACTTTTTACTATCAACAAAAGGTACTGCTGGCAGATGACAAATACGCCGGGCTGAA
AGACCTGATTCAGGCAACTTTCTACGAGCACAAGGGCCGGTATGGCTATCGTCGCATCACGGCAGCTTTGCGCAGGGCGGGCCATCTTGTGAACCACAAG
ACGGTGCAGAAACTAATGGGGCAGCTAGGCCTGAAGAGCCTGATACGCGTGAAGAAATACCGTTCCTACAAAGGCGAAACAGGCAAGGCCGCGCCTAACC
TGCTAAAGCGTGATTTCAAAGCGCAATACCTTAACCAGAAATGGGCCACCGACGTGACCGAATTCAAAGTAGGAGGCCAGAAACTCTATCTGTCGCCGAT
CATGGATCTGTACAGCGGCGAAATCATTTCCTATGCGATTGCCAGGCGCCCGCTGTACTCCATGGTCGACGAAATGCTTGAAGGAGCATTTAAGCGGCTT
GGACCGCATGAAAAGCCGATTTTGCATTCCGACCAGGGCTGGCAATATCGGATGCCTATTTACCAGCGACTGCTCAATGAGAATTCGATCACAGCCAGCA
TGTCGCGCAAGGGTAACTGCTACGACAACGCGGCTATTGAGAGTTTTTTTAGCACGCTGAAATTCGAATTTTTCTACCTGAACAAATTTAACGATCTGGA
TGAGCTTCAAGCGGGTATCGAAGAGTACATTGAGTATTACAATCACTCACGCATAAAACTGAAGCTTAACGGCCTGAGTCCTGTGGAATACAGAATGCAG
GCCGCCAAGGCAGCGTAGGTGGATAATTGTCCAACCTTCGGGGGTCACACCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 540 bp | 179 aa | 43 | 582 | + | No |
Description : First part of the transposase
ORF sequence :
MGKYTEQAKLAAVKEYCAGKAGLRDVAHRHDVDFSCLRQWVAAYQIHGPAALKEKRRQRYSDEFKLAVLKRMHDERLSLRQTAALFDIRQFGIINLWQRL
FDEGALNASSKPPKKAGRPRKMTTPLPPVNSSPINDESRSRDELLAEVKQLRMEVDYLKKLDALVQKKQRIAQQKKRKS
FDEGALNASSKPPKKAGRPRKMTTPLPPVNSSPINDESRSRDELLAEVKQLRMEVDYLKKLDALVQKKQRIAQQKKRKS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 315 aa | 468 | 1418 | + | No |
Description : Second part of the transposase
ORF sequence :
RVACRGEATADGGRLSKKARCLGEEATNSAAEKAQIVMELRPLHSLDGLLKFAGLARSTFYYQQKVLLADDKYAGLKDLIQATFYEHKGRYGYRRITAAL
RRAGHLVNHKTVQKLMGQLGLKSLIRVKKYRSYKGETGKAAPNLLKRDFKAQYLNQKWATDVTEFKVGGQKLYLSPIMDLYSGEIISYAIARRPLYSMVD
EMLEGAFKRLGPHEKPILHSDQGWQYRMPIYQRLLNENSITASMSRKGNCYDNAAIESFFSTLKFEFFYLNKFNDLDELQAGIEEYIEYYNHSRIKLKLN
GLSPVEYRMQAAKAA
RRAGHLVNHKTVQKLMGQLGLKSLIRVKKYRSYKGETGKAAPNLLKRDFKAQYLNQKWATDVTEFKVGGQKLYLSPIMDLYSGEIISYAIARRPLYSMVD
EMLEGAFKRLGPHEKPILHSDQGWQYRMPIYQRLLNENSITASMSRKGNCYDNAAIESFFSTLKFEFFYLNKFNDLDELQAGIEEYIEYYNHSRIKLKLN
GLSPVEYRMQAAKAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1366 bp | 457 aa | 53 | 1418 | + | Yes |
Chemistry : DDE
ORF sequence :
MGKYTEQAKLAAVKEYCAGKAGLRDVAHRHDVDFSCLRQWVAAYQIHGPAALKEKRRQRYSDEFKLAVLKRMHDERLSLRQTAALFDIRQFGIINLWQRL
FDEGALNASSKPPKKAGRPRKMTTPLPPVNSSPINDESRSRDELLAEVKQLRMEVDYLKKARCLGEEATNSAAEKAQIVMELRPLHSLDGLLKFAGLARS
TFYYQQKVLLADDKYAGLKDLIQATFYEHKGRYGYRRITAALRRAGHLVNHKTVQKLMGQLGLKSLIRVKKYRSYKGETGKAAPNLLKRDFKAQYLNQKW
ATDVTEFKVGGQKLYLSPIMDLYSGEIISYAIARRPLYSMVDEMLEGAFKRLGPHEKPILHSDQGWQYRMPIYQRLLNENSITASMSRKGNCYDNAAIES
FFSTLKFEFFYLNKFNDLDELQAGIEEYIEYYNHSRIKLKLNGLSPVEYRMQAAKAA
FDEGALNASSKPPKKAGRPRKMTTPLPPVNSSPINDESRSRDELLAEVKQLRMEVDYLKKARCLGEEATNSAAEKAQIVMELRPLHSLDGLLKFAGLARS
TFYYQQKVLLADDKYAGLKDLIQATFYEHKGRYGYRRITAALRRAGHLVNHKTVQKLMGQLGLKSLIRVKKYRSYKGETGKAAPNLLKRDFKAQYLNQKW
ATDVTEFKVGGQKLYLSPIMDLYSGEIISYAIARRPLYSMVDEMLEGAFKRLGPHEKPILHSDQGWQYRMPIYQRLLNENSITASMSRKGNCYDNAAIES
FFSTLKFEFFYLNKFNDLDELQAGIEEYIEYYNHSRIKLKLNGLSPVEYRMQAAKAA
Blast result :
Comments
There is only one copy of ISPsy9 in this strain.
References