ISAs3

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) ISAs11
Accession numberTranspositionOriginHost
NC_004338 ND Aeromonas salmonicida
Aeromonas salmonicida subsp. salmonicida plasmid pAsal1
DNA section
IS Length : 1326 bp

Ends


IR Length : 26/32

IRL : GAGCCTGTAAATCCAATTGTGTAACCGCCCACGAATAAGTAATTTTTTCA
IRR : GAGCCTGTAAATAATTCTGTGTAACTGCCCACTCCATTACAGGTGACCGT

Insertion site


Left flankDirect repeatRight flankDR Length
TTCCGGAGAGAGGGAGGGGCTTGTTTTC8

DNA sequence

GAGCCTGTAAATCCAATTGTGTAACCGCCCACGAATAAGTAATTTTTTCAAAAGGATACCTTCGTGGGTAAGCAAATGGACCAAGACAAACTCAAAGCAC
TGGCCGCTGAACTGGCCAAAGACATCAAGTCTGAAAAAGATCTCGGCACCCTCACCCAACAGCTGATCAAGCTCACCGTCGAGACCGCGCTCAATGCCGA
GATGGACGAGCATCTGGGCTACGAGAAACACGCTCCACAAGGCCGCGGGACCGGCAACAATCGCAATGGCTATTCCACCAAGCGCCTAAAAGGCCAGCAC
GGAGAGGTCACCATCCAGGCGCCTCGCGACCGTAACGCCTCCTTCGAGCCTCAGTTCGTCCGCAAGGGCCAGTCCCGCCTGACCCAGATGGACGACCAGA
TCCTCGCCCTCTACGCCAAGGGCTTGAGTACGCGGGATATCGTGGACGCGTTCAAAGAGATGTATGACGCCGACATCTCGGCGGGGCTGGTATCGAAGGT
GACCGAACGTGTCATCGAGCAGGTCCACGAATGGCAAAACCGGCCGCTGGATCCGCTCTATCCCATCGTCTATCTGGACTGCATTGTGCTGAAGATCCGC
GCGAACCAGCGGGTGATCAACAAGTCGCTCTATTTGGCTCTGGGCATCAATATGGAGGGGCACAAGGAGCTGTTGGGCCTGTGGCTGGCCGAGACCGAAG
GGGCAAAGTTCTGGTTGTCGGTCCTGACCGAGCTGAAGAACCGCGGCCTGGAAGACATCCTGCTCGCGTGTGTGGATGGTCTCAAGGGTTTCCCTGATGC
CATTGCCGTGGAGTATCCCCAGACCAAGGTGCAACTGTGTATCGTCCATATGGTCCGCAACTCGTTGCGCTATGTCTCCTGGAAGGACTACAAGGCGGTG
ACGGCTGAACTGAAGCAGATCTACCAGTCTGCGACGGAACGTGAGGCTCAGCAGGCGTTAGCGACCTTCGGCGAGCGCTGGGATAGCCAGTACCCGCAGA
TAGCGCGCTCGTGGCAGAATAACTGGGACAATCTGATCACGCTGTTTGATTATCCCCCCGCCATCTGGAAGGTAATTTACACAACCAATGCGATCGAGTC
ACTCAACAGCGTGCTGCGCAAAGCGACCAAACAAAGGAAGCTGTTCCCGACCGATGAGTCGGCGCTCAAAGTGGCGTTTCTCGCGATACAGCAGGCTTCA
AAGAAGTGGACCATGCCAATCCAGAATTGGAAGCCTGCCCTGAATCGCTTTATTATCGAGTTCGGTGATCGCCTGAACGGTCACCTGTAATGGAGTGGGC
AGTTACACAGAATTATTTACAGGCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1215 bp404 aa761290+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDQDKLKALAAELAKDIKSEKDLGTLTQQLIKLTVETALNAEMDEHLGYEKHAPQGRGTGNNRNGYSTKRLKGQHGEVTIQAPRDRNASFEPQFVRKGQS
RLTQMDDQILALYAKGLSTRDIVDAFKEMYDADISAGLVSKVTERVIEQVHEWQNRPLDPLYPIVYLDCIVLKIRANQRVINKSLYLALGINMEGHKELL
GLWLAETEGAKFWLSVLTELKNRGLEDILLACVDGLKGFPDAIAVEYPQTKVQLCIVHMVRNSLRYVSWKDYKAVTAELKQIYQSATEREAQQALATFGE
RWDSQYPQIARSWQNNWDNLITLFDYPPAIWKVIYTTNAIESLNSVLRKATKQRKLFPTDESALKVAFLAIQQASKKWTMPIQNWKPALNRFIIEFGDRL
NGHL

 

Blast result :
Comments
ISAs3 is 84% aa similar to IS1414.
References
1] Fehr,D (2002) Direct submission