ISPpu13
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_002947 | ND | Pseudomonas putida | Pseudomonas putida KT2440 |
DNA section
IS Length : 2370 bp
Ends
IR Length : 19/22
IRL : GTAAGCGCTCCATGAACCCCACATTCAAAGCGCTTACCTGATCCCCGATG
IRR : GTAAGCGATCCATAAACCCAACCCGTTACATGAAGGACGGGAAAATCAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAGGCCTGGC | ATTGGCTC | AAGCGACGGC | 8 |
| TCAATGCGCT | GAAGCGTTGC | 0 |
DNA sequence
GTAAGCGCTCCATGAACCCCACATTCAAAGCGCTTACCTGATCCCCGATGCTGTGCTCCCGATTTCTTTCTGGAGCTCGCCGACCATGATGCGACCCGAC
GCCAAAGTCGAAAAAGTGTACCTCTACCCCAAGCCCGTCGACTTTCGAAAATCCATCGATGGCCTGGCCGCGCTGGTCGAACTCGATATCAAGGTGGCGG
TGTTCGACCCAGTGCTTTTCGTCTTCCTCAACAAGCCTCGCAATCGCGTGAAAATCCTTTATTGGGAGCGAAACGGCTTCTGCCTCTGGCTCAAGCGCCT
GGAGTCCGAACGTTTCAAAACTTCACCTGATCCGACTGACGTCGCCATTGTCCTGACCGTTCAGGAACTGAACTGGCTGCTCGATGGCTTCGACCTCTGG
CGCAACCGTCCGCATCAGGTTTTGACGCCTCGATACGTCGCCTGATTCGGTATAATCCGCGGCATGATTTCCATGCCCGAAAACCTCCCTGACGATCCGG
TTCTGCTCAAGCAACTGCTTGAGCAGATGATCCACGAGCGCGCGTCCGACAAGAGCAAGATCGTTCATCTCGAAGAGGAAGTTGCGCTGTTGCGCCAGCG
CTTGTTCGGGCGCAAGACCGAGCAGACTGGCGATGCGGCGACACCACAGTTGCCACTCTTCGACGAAGCGGAAAGCCTGGCCGAGCCTTTGGATGAGGCT
GACGACGAAGAAGTCGTTGCGCCAACCAAACGCCGTGGCAAGCGCAAGCCGCTGCCAGCTGATTTGCCACGTATCGAAGTCGTCCACGAACTGCCCGAGC
ATGAACTGACCTGCGCTTGTGGCTGCCGTAAACATGCCATCGGTGAAGAAGTCAGCGAACAACTTGAAATCGTCCCGATGCAGATTCGCGTCATCAAACA
TGTTCGCAAGGTGTACGGCTGCCGGGACTGCGAGTCAGCGCCCGTAACGGCGGACAAGCCCGCCCAGATGATCGAGAAAAGCATGGCCAGCCCGAGTGTG
CTGGCCATGCTGCTGACCACCAAATACGTAGACGGCTTGCCGCTTCACCGCTTCGAAAAAGTGCTGGGGCGTCACGGTATCGATATCCCGCGCCAAACCC
TGGCACGCTGGGTGATTCAATGCAGCGAGCACTTTCAGCCGCTGCTGAATCTGATGCGCGAAAGCCTGTTGAAGAGTCGGATCATCCACTGTGATGAAAC
ACGTGTGCAGGTGTTGAAAGAGCCGGGGCGTGAGCCCAGCAGCCAATCCTGGATGTGGGTACAAACCGGCGGCCCACCTGATCGACCAGTGATCCTTTTC
GACTACGCCACCAGCCGAGCGCAGGAGGTGCCGGTGCGCCTGCTGGACGGCTATCGCGGCTATGTCATGACCGACGACTACGCCGGTTACAACGCGCTGG
CTGCACAGGACGGGCTGGAGCGATTGGGCTGCTGGGCGCACGCGCGCCGCAAGTTCGTCGAAGCGCAGAAAGTACAGCCCAAGGGCAAAACAGGACGCGC
TGACATCGCGTTGAACCTGATCAATAAGCTCTATGGCGTCGAGCGCGACTTGAAGGACAGTGACGACGAAGATCGTAAGGTTGCCCGCATGGAGCGCAGC
CTGCCATTGCTGACTCAGCTGAAAAACTGGGTAGAGAAAACGCAACCTCAGGTCACGACGCAGAATGCCTTGGGCAAGGCCATCGGTTACTTGGCCAGCA
ACTGGAGCAAATTGGAACGCTACGTCGAGCACGGCTACTTGCCGATGGACAACAACGCGGCCGAGCGTGCGATCCGCCCGTTTGTCATCGGGAGAAAGAA
CTGGTTGTTCAGTGACACGCCCAGAGGGGCGACGGCCAGTGCGCAACTTTACAGCCTGGTCGAGACGGCCAAAGCCAACGGCCAAGAGCCCTATGCGTGG
CTGCGCCACGCACTAGAGCGCCTGCCGCAGGCGGCCTCCGTGGAAGACTACGAAGCGCTGCTGCCGTGGAATTGCAAGCCTAAAATACATAGTTAAGTGC
TACCCCCATCGTGGGTCAGAAGGGGGCCTACGAAGCGCTGAAGGACAAGAGCGGGGTGACGACCATGGAATACGACGAAAAACTGATTGAAGACGCAGTG
CTTGCCTTGTTGGCAGCCTATAGCTCCGACAAAGGTAACGCGTGGAAAGGATTTGACTTCGAGATCATGAATCGACTGCATGAACAGGGTTTCATCAGTG
ATCCAGTTAATAGAAACAAATCCATCTGGTTGACGGCGGAGGGGCTGGAGCGAGGCCGACAGCTAGCTGACCAGTTGTTCGGGTTGAAGAGTCAAGCGGG
GCAGGTGCCCGACTCGAATACCTGATTTTCCCGTCCTTCATGTAACGGGTTGGGTTTATGGATCGCTTAC
GCCAAAGTCGAAAAAGTGTACCTCTACCCCAAGCCCGTCGACTTTCGAAAATCCATCGATGGCCTGGCCGCGCTGGTCGAACTCGATATCAAGGTGGCGG
TGTTCGACCCAGTGCTTTTCGTCTTCCTCAACAAGCCTCGCAATCGCGTGAAAATCCTTTATTGGGAGCGAAACGGCTTCTGCCTCTGGCTCAAGCGCCT
GGAGTCCGAACGTTTCAAAACTTCACCTGATCCGACTGACGTCGCCATTGTCCTGACCGTTCAGGAACTGAACTGGCTGCTCGATGGCTTCGACCTCTGG
CGCAACCGTCCGCATCAGGTTTTGACGCCTCGATACGTCGCCTGATTCGGTATAATCCGCGGCATGATTTCCATGCCCGAAAACCTCCCTGACGATCCGG
TTCTGCTCAAGCAACTGCTTGAGCAGATGATCCACGAGCGCGCGTCCGACAAGAGCAAGATCGTTCATCTCGAAGAGGAAGTTGCGCTGTTGCGCCAGCG
CTTGTTCGGGCGCAAGACCGAGCAGACTGGCGATGCGGCGACACCACAGTTGCCACTCTTCGACGAAGCGGAAAGCCTGGCCGAGCCTTTGGATGAGGCT
GACGACGAAGAAGTCGTTGCGCCAACCAAACGCCGTGGCAAGCGCAAGCCGCTGCCAGCTGATTTGCCACGTATCGAAGTCGTCCACGAACTGCCCGAGC
ATGAACTGACCTGCGCTTGTGGCTGCCGTAAACATGCCATCGGTGAAGAAGTCAGCGAACAACTTGAAATCGTCCCGATGCAGATTCGCGTCATCAAACA
TGTTCGCAAGGTGTACGGCTGCCGGGACTGCGAGTCAGCGCCCGTAACGGCGGACAAGCCCGCCCAGATGATCGAGAAAAGCATGGCCAGCCCGAGTGTG
CTGGCCATGCTGCTGACCACCAAATACGTAGACGGCTTGCCGCTTCACCGCTTCGAAAAAGTGCTGGGGCGTCACGGTATCGATATCCCGCGCCAAACCC
TGGCACGCTGGGTGATTCAATGCAGCGAGCACTTTCAGCCGCTGCTGAATCTGATGCGCGAAAGCCTGTTGAAGAGTCGGATCATCCACTGTGATGAAAC
ACGTGTGCAGGTGTTGAAAGAGCCGGGGCGTGAGCCCAGCAGCCAATCCTGGATGTGGGTACAAACCGGCGGCCCACCTGATCGACCAGTGATCCTTTTC
GACTACGCCACCAGCCGAGCGCAGGAGGTGCCGGTGCGCCTGCTGGACGGCTATCGCGGCTATGTCATGACCGACGACTACGCCGGTTACAACGCGCTGG
CTGCACAGGACGGGCTGGAGCGATTGGGCTGCTGGGCGCACGCGCGCCGCAAGTTCGTCGAAGCGCAGAAAGTACAGCCCAAGGGCAAAACAGGACGCGC
TGACATCGCGTTGAACCTGATCAATAAGCTCTATGGCGTCGAGCGCGACTTGAAGGACAGTGACGACGAAGATCGTAAGGTTGCCCGCATGGAGCGCAGC
CTGCCATTGCTGACTCAGCTGAAAAACTGGGTAGAGAAAACGCAACCTCAGGTCACGACGCAGAATGCCTTGGGCAAGGCCATCGGTTACTTGGCCAGCA
ACTGGAGCAAATTGGAACGCTACGTCGAGCACGGCTACTTGCCGATGGACAACAACGCGGCCGAGCGTGCGATCCGCCCGTTTGTCATCGGGAGAAAGAA
CTGGTTGTTCAGTGACACGCCCAGAGGGGCGACGGCCAGTGCGCAACTTTACAGCCTGGTCGAGACGGCCAAAGCCAACGGCCAAGAGCCCTATGCGTGG
CTGCGCCACGCACTAGAGCGCCTGCCGCAGGCGGCCTCCGTGGAAGACTACGAAGCGCTGCTGCCGTGGAATTGCAAGCCTAAAATACATAGTTAAGTGC
TACCCCCATCGTGGGTCAGAAGGGGGCCTACGAAGCGCTGAAGGACAAGAGCGGGGTGACGACCATGGAATACGACGAAAAACTGATTGAAGACGCAGTG
CTTGCCTTGTTGGCAGCCTATAGCTCCGACAAAGGTAACGCGTGGAAAGGATTTGACTTCGAGATCATGAATCGACTGCATGAACAGGGTTTCATCAGTG
ATCCAGTTAATAGAAACAAATCCATCTGGTTGACGGCGGAGGGGCTGGAGCGAGGCCGACAGCTAGCTGACCAGTTGTTCGGGTTGAAGAGTCAAGCGGG
GCAGGTGCCCGACTCGAATACCTGATTTTCCCGTCCTTCATGTAACGGGTTGGGTTTATGGATCGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 360 bp | 119 aa | 86 | 445 | + | No |
AG : IS66 TnpB
ORF sequence :
MMRPDAKVEKVYLYPKPVDFRKSIDGLAALVELDIKVAVFDPVLFVFLNKPRNRVKILYWERNGFCLWLKRLESERFKTSPDPTDVAIVLTVQELNWLLD
GFDLWRNRPHQVLTPRYVA
GFDLWRNRPHQVLTPRYVA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1533 bp | 510 aa | 464 | 1996 | + | No |
Chemistry : DDE
ORF sequence :
MISMPENLPDDPVLLKQLLEQMIHERASDKSKIVHLEEEVALLRQRLFGRKTEQTGDAATPQLPLFDEAESLAEPLDEADDEEVVAPTKRRGKRKPLPAD
LPRIEVVHELPEHELTCACGCRKHAIGEEVSEQLEIVPMQIRVIKHVRKVYGCRDCESAPVTADKPAQMIEKSMASPSVLAMLLTTKYVDGLPLHRFEKV
LGRHGIDIPRQTLARWVIQCSEHFQPLLNLMRESLLKSRIIHCDETRVQVLKEPGREPSSQSWMWVQTGGPPDRPVILFDYATSRAQEVPVRLLDGYRGY
VMTDDYAGYNALAAQDGLERLGCWAHARRKFVEAQKVQPKGKTGRADIALNLINKLYGVERDLKDSDDEDRKVARMERSLPLLTQLKNWVEKTQPQVTTQ
NALGKAIGYLASNWSKLERYVEHGYLPMDNNAAERAIRPFVIGRKNWLFSDTPRGATASAQLYSLVETAKANGQEPYAWLRHALERLPQAASVEDYEALL
PWNCKPKIHS
LPRIEVVHELPEHELTCACGCRKHAIGEEVSEQLEIVPMQIRVIKHVRKVYGCRDCESAPVTADKPAQMIEKSMASPSVLAMLLTTKYVDGLPLHRFEKV
LGRHGIDIPRQTLARWVIQCSEHFQPLLNLMRESLLKSRIIHCDETRVQVLKEPGREPSSQSWMWVQTGGPPDRPVILFDYATSRAQEVPVRLLDGYRGY
VMTDDYAGYNALAAQDGLERLGCWAHARRKFVEAQKVQPKGKTGRADIALNLINKLYGVERDLKDSDDEDRKVARMERSLPLLTQLKNWVEKTQPQVTTQ
NALGKAIGYLASNWSKLERYVEHGYLPMDNNAAERAIRPFVIGRKNWLFSDTPRGATASAQLYSLVETAKANGQEPYAWLRHALERLPQAASVEDYEALL
PWNCKPKIHS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 315 bp | 104 aa | 2011 | 2325 | + | No |
Annotation : Description :
ORF sequence :
MGQKGAYEALKDKSGVTTMEYDEKLIEDAVLALLAAYSSDKGNAWKGFDFEIMNRLHEQGFISDPVNRNKSIWLTAEGLERGRQLADQLFGLKSQAGQVP
DSNT
DSNT
Blast result :
Comments
There are 2 copies of ISPpu13 in this strain, and one copy lacks a target site duplication.
ISPpu13 is 69% aa (ORFA) similar to IS1326 , 50% (ORFB : the transposase) to ISRm14. The third ORF is a passenger gene annoated as hypothetical protein.
ISPpu13 is 69% aa (ORFA) similar to IS1326 , 50% (ORFB : the transposase) to ISRm14. The third ORF is a passenger gene annoated as hypothetical protein.
References
1] DeBoy., R. (2002) Direct submission