ISfinder Mirror Site

ISW1

Family IS200/IS605
Group IS200

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AB020533	ND	Wolbachia sp.	Wolbachia sp. wTai

DNA section

IS Length : 572 bp

Ends

Left end : TTTATTGTCCCAAATTTGGGAGAAAAACCCTGGACTTGAAGTCCATCCTTTTAAGAATATAATTTTAAGGTGAAAAATCTGAAAGAATGTGGAATATTAT II struct. : No
Right end : AATCACTCTAAAAAAGAAGATAAATTTGGTGACTTTCAAGTCGAAAGTTAGCATTCTATGCTCTCTCTCAATCTTTGGATTTTAATCCATAGTGGTTGAA II struct. : No

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
	ttat		tgaa

DNA sequence

TTTATTGTCCCAAATTTGGGAGAAAAACCCTGGACTTGAAGTCCATCCTTTTAAGAATATAATTTTAAGGTGAAAAATCTGAAAGAATGTGGAATATTAT
AGAAAAAGCAGTCACTCTGTATATGAATGTACATATCACATAGTGTGGATAACAAAATATCGATATCCAGTACTAGTTGGAGATATTGGACAGAAAGCTA
AAGAAGTTGTACAAGAAGTATGTGCAAATAATCAAGTAGAAATTATCAGAGGTAGAGTTGCGTCAAGTCATGTACACGTATACGTTTCTGTACCCCCATA
CTTAAGTATTAGTAAGTTAGTGCAGTTAATAAAAGGAAAAAGTTCCAGAAAAATCCAGCAAAACTTTCCAGAGCTAAAAAAGAGATACTGGGGAAAACAC
TTGTGGGCAGTAGGCTACTTTGTCAGGACTAGCGGAAATGTAACTGACGAAATGATAAAAAATTATATTGAAAATCACTCTAAAAAAGAAGATAAATTTG
GTGACTTTCAAGTCGAAAGTTAGCATTCTATGCTCTCTCTCAATCTTTGGATTTTAATCCATAGTGGTTGAA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
435 bp	144 aa	89	523	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MEYYRKSSHSVYECTYHIVWITKYRYPVLVGDIGQKAKEVVQEVCANNQVEIIRGRVASSHVHVYVSVPPYLSISKLVQLIKGKSSRKIQQNFPELKKRY
WGKHLWAVGYFVRTSGNVTDEMIKNYIENHSKKEDKFGDFQVES

Blast result :

Comments

ORF is 32-39% identity to IS200 and 60% to IS1004. There is at least 20 copies in the Wolbachia wTai genome of which 9 have been analysed and have an identical sequence.

References

1] Masui, S., Kamoda, S., Sasaki, T., and Ishikawa, H. (1999) Plasmid 42, 13-19.