IST2

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
J03859 ND Thiobacillus ferrooxidans
Thiobacillus ferrooxidans ATCC19859
DNA section
IS Length : 1408 bp

Ends


IR Length : 22/28

IRL : GAGCTATGCTCGAAAGTGGTGTATGGGCGCGTTACAACTGGCTTGAATGA
IRR : GAGCTATAGTCAAATCTGGTGTATGAGCATTTTCTGACATAGATCAGGCG

Insertion site


Left flankDirect repeatRight flankDR Length
ACGTGTCGCTCGCGGCGGCAGCCGGCAAC9
CGTCCAGTAACTGTGAGGACCTCAGAATG9
NNNNNNNNNNCCGGTTGGTATGGCTATCC9
TGCTCTATCTCATGCTCGCNNNNNNNNNN9
NNNNGACTTGATCATTCCCTCCGATNNNN9
NNNNCGCCCTGATCATGGGTCACGTNNNN9

DNA sequence

GAGCTATGCTCGAAAGTGGTGTATGGGCGCGTTACAACTGGCTTGAATGAGGTGGCGTACTGTTGCTACAAAGGGTTTCCGACTCAAAGAGCAACATAAG
GAGTACGCCGTGGAAAAGAATACCGTAATAGCAGGTGGGATGGGAGAGTTGGGTCTGGGCATTGAAGGGATACTTCGACGCGCGGCACTCTGCATTGAGC
AGGCCATAGAGGCAGAGGTGGCGGTATTGCTGGAAGAATTTGCGACGGTGCGGATGGTTGATGGGCGTCAGGCGGTCGTGCGTAATGGGCATCTGCCGGA
GCGCGAGATCATGACCGCTCTGGGTCCGGTACCCGTCAAAGTACCCAAGGTGCGGGACCGTCAGGATCGGGGATCAAATTCAATTCGGTACTGGCGCCTC
CGTATGTACGCAAATCACGAACGGTAGCCGCTACAGTACCTTGGCTCTATCTGCATGGGGTGTCTTCCGGCCACATGCAGGAAGCCCTTTCCATTTTGCT
GGGTGATGAGGCCAAGGGACTTTCGCCTGCGGTGTTGGGACGTCTCAAGGCGGAGTGGGCGCAAGAGTATGCCCATTGGCAACGCCGCTCCTACAGGAAA
GCGCTATGCTTACTGGTGGGTAGACGGTATTTATACGAACCTCCGTGCGGAGAGGATCCGCGTATCTGCCTGCTGGTGATTATCGGCGTGACGGCAGAGG
GCAAGAAAGAGCTGGTCATGGTCAGTGACGGCCTGCGCGAATCCAAAGCTTCCTGGCTGGAGATCCTGCGTGACCTGCAGGCGCGCGGTCTGGAGACGGC
CCCTTTGCTCGCCATTGGGGATGGTGCCATGGGGTTTTGGGCCGCACTGGATGAAGCCTATCCCGAAACTGGTCAGCAACGCTGCTGGGTGCATAAGACC
GCCAACATTCTCAACGAACTTCCCAAAGCCCAACAGAGCAAAGCCAAGGCAGCGTTGCAGGAAATCTGGATGGCCGCCAATCGCCAGGCTGCGGAAAAAG
CACTGGACGTGTTTGTGCGCAATTACCAGGCAAAGTATCCCAAGGCTGTGGCTAAACTGGAAAAAGATCGGGCTGAATTGCTCGCTTTCTATGATTTTCC
GGCCGAACACTGGCGGCATATCCGGACCACCAATGCCATTGAGTCCACCTTCGCTACGGTACGCCACCGGACTACCCGGACGAAGAACTGTGTATCACGC
AGCAGCTTTCTGGGATTGGGTTTCAAGATGCTGCAGCAGGCTGAAAAACGCTGGATTGGGATTTATGCTCCAGAGAAAGTCCTGCAGCTTTTTGCAGGGG
TGAAATTTATCGATGGCATACCGGCTAACCTCACCCTGCCGGATGATCAACAGACCGCCGCCTGATCTATGTCAGAAAATGCTCATACACCAGATTTGAC
TATAGCTC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1319 bp438 aa761394+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRWRTVATKGFRLKEQHKEYAVEKNTVIAGGMGELGLGIEGILRRAALCIEQAIEAEVAVLLEEFATVRMVDGRQAVVRNGHLPEREIMTALGPVPVKVP
KVRDRQDRGSNSIRYWRLRMYANHERAATVPWLYLHGVSSGHMQEALSILLGDEAKGLSPAVLGRLKAEWAQEYAHWQRRSYRKALCLLVGRRYLYEPPC
GEDPRICLLVIIGVTAEGKKELVMVSDGLRESKASWLEILRDLQARGLETAPLLAIGDGAMGFWAALDEAYPETGQQRCWVHKTANILNELPKAQQSKAK
AALQEIWMAANRQAAEKALDVFVRNYQAKYPKAVAKLEKDRAELLAFYDFPAEHWRHIRTTNAIESTFATVRHRTTRTKNCVSRSSFLGLGFKMLQQAEK
RWIGIYAPEKVLQLFAGVKFIDGIPANLTLPDDQQTAA

 

Blast result :
Comments
IST2 contains two overlapping ORFs: ORF1 (76-453) and ORF2 (M=504-1394). These ORF have been considered as forming a single ORF, based on comparisons to the other members of the IS256 family (the intervening sequence between the two putative proteins contains highly conserved residues !). However, this will require a frameshift around position 430-470 in the current DNA sequence. This proposal needs further experimental confirmations.
References