ISfinder Mirror Site

ISSfl3

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AL391753	ND	Shigella flexneri	Shigella flexneri

DNA section

IS Length : 2729 bp

Ends

IR Length : 11

IRL : GTAAGCGCCCCATCAGCGACGTCTTGTGAAAATTGTCCTGTCTGGCAACA
IRR : GTAAGCGCCCCGAGAAGTACGTAGCGTAAGGATTATTTTACAGACGAGAA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGTGAAAACG		GTTGATGCAG	0
CGGGATTAAT		CTTCAAAATG	0

DNA sequence

GTAAGCGCCCCATCAGCGACGTCTTGTGAAAATTGTCCTGTCTGGCAACAATCGCGCCCATCTATATTGATGGACACGAACGATGAATTCCCAGACAACA
AAAGATATTCCCTGCTTCCGTTCTTATTTGCCTGATGCCCTGCGTTTAAGATTTGAAGATAAACTGACCATCCGGGCCATCGCTCAGCGTCTGGGTCTCA
GTCATTCCACAATACATACGCTTTTTCAGCGATTTCTTGCATCCGGTATCGCATGGCCATTGCCCGATTCAGTTTCATTCGCTCAACTTGACGCCATCCT
TTATGCCAACAGAAAGAAGGAATTAACAGAGCCTCAAATCAGAGAAGGCTCATGGCGAAAAGAACGGCGAACCAGCTATAGCCGTGAATTTAAGGTCCGT
CTGGCTAAGCAGGCATTACAGCCTGGGGCTGTTGTTGCCCGGATCGCCAGAGAACACGATATCAATGATAACCTGCTGTTTAAATGGAAAAGCCAGTACG
AGGACGGCTTACTGAGCGATGATGACATACAGGAATGCATGCCTGTCCCGGTGGCACTGACTGATACGCCGGAGCCGACCAGACCAGTTACAAATCCCTT
CTGGCGTAACAAGCATGATGAGCGCCCTGAGGGGGCTCCCGGAAACGTCCCACGGTGCGAGCTGCATCTTAAATCAGGTGTGGTAAAACTGTTTGACCCT
CTCACTCCGGAACTGTTACGGGCGCTAATCCGCGAAATGAAAGGGGGTATCCGATGATAACGCTGCCGACCGGTACCAAAATCTGGATCATCGCTGGCAT
CACAGATATGCGTTGTGGCTTCAATGGCCTGGCTTCGAAGGTGCAGAACACGCTGAAAGATGACCCGTTCTCCGGGCATATCTTCGTCTTCCGGGGCCGC
AGTGGCAAAATGGTGAAAATACTGTGGGCCGATCGTGACGGGTTATGCCTGTTCACCAAACGTCTGGCCGGTGACCCGGGAAGGGAAAGTGCACCTGACG
CCAGCTCAGTTATCCATGCTACTGGAGGGGATCGCGTGGCAACATCCCAAACGGACAGAACGGCCTGGCATCCGGATATAACCCGTGATAAAACAAGGGA
ATGAACAATGAACTCCCCGATGATATTGAGCTGCTTAAAGCCATGTTGCGTAAGCAACAGAGTCGGCTTCGACAGTATGCCTGTCAGGTCGCGGGCTATG
AGCAGGAAATTGAACGGCTGAAAGCGCAACTCGACAGGTTGCGTCGTATGTTGTTCGGCCAGAGTTCAGAGAAAAAGCGTCATAAGCTTGAAAATCAGAT
CCGACAGGCAGAAAAACGACTGTCGGAACTGGAAAACCGGCTGAACACAGCCAGAAATCTTCTGGAAGATGCATCGTCAGTCACAGATTCACCTGACACC
AGTCCCCCGTCAGAAAACCCGATCGCCAGTAAGCCTGAATCCCCGGGACGAAAATCTTCACGAAAACCGCTGCCGGCAGAACTTCCCCGGGAGACACATC
GCCTTCTGCCTGCTGAAACCAGTTGCCCGGCCTGTGGAGGTGTTCTGAAAGAAATGGGGGAAACAATCTCAGAGCAACTGGATATCATTAATACCGCCTT
TAAAGTTATCGAAACCATACGTCCCAAACTGGCCTGTAGCCGGTGTGATGTCATCGGCACCACTTCCTCCTAAACCGATCGAACGCGGTTATGCCAGTGC
AGGGTTACTTGCACGGATCCTGGTCAGCAAATATATGGAACATATCCCTTTATATCGCCAGTCAGAAATATACGCGCGACAGGGCGTGGAGCTGAGCCGT
AATACCATGGTGCGCTGGGTATCAGAAATGGCAGACAAACTCCGTCCTCTGTATATAGCGCTGAATGACTATGTTCTGGAGGCAGGAAAGGTGCACGCAG
ATGACACTCCGGTGAAAGTACTGGCCCCGGGGAACGGAAAGACGAAAACGGGTCGTCTGTGGGTATACGTCAGGGATGATCGTAATGCGGGTTCATCCCT
GCCGGCAGCCGTCTGGTTCGCGTATTCGGCAGATCGCAAAGGAGAACATCCGCAGCTCCACCTGGCAAAGTATCAGGGCGTACTGCAGGCTGATGCCTAT
GCAGGTTATAACGTACTGTACGAAACGGGCCGGGTGAAGGAAGCCGGGTGTCTGGCCCACGCCCGCCGAAAAATCCATGACGAGGATGTGCGCCGTCCGA
CAGAAATGACTCAGGAAGCGCTCAGACGGATAGCAGAGTTATACGACATAGAAGCGGAGATACGTGGCAGTCCGGCAGAGGAACGGCTTGCAGTCAGAAA
AGCCAGAAGCGTCCAGTTGATGCAGTCGTTGTACGACTGGATACAGTTGCAGAGGAAAACGCTGTCGAAACATGCGGAGATGGCGAAGGCGTTCGACTAT
ATCCTGAATCACTGGAATGCGCTGAACGAGTTCTGTCGTGACGGCTGGGTGGAAATAGACAACAACATCGGTGAAAACGCGTTACGATCGGTGGCGGTTG
GAAGAAAAAATTATCTCTTTTTCGGCTCAGACAAGGGAGGAGAAAGTGCGGCGATCATCTACAGTCTGCTGGTCACCTGCAAACAGAACGAAGTGGAGCC
GGAGGACTGGTTGCGCGAAGTGATCGAGAAGCTCAATGACTGGCCGTCGAACCAAGTGCATGAACTGCTGCCCTGGAACTTCTCGTCTGTAAAATAATCC
TTACGCTACGTACTTCTCGGGGCGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
675 bp	224 aa	83	757	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTIHTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYS
REFKVRLAKQALQPGAVVARIAREHDINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGV
VKLFDPLTPELLRALIREMKGGIR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
328 bp	109 aa	754	1081	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIFVFRGRSGKMVKILWADRDGLCLFTKRLWPVTREGKVHLTPAQLSMLLEGIAWQHPK
RTERPGIRI

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1597 bp	531 aa	1101	2697	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDT
SPPSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINTAFKVIETIRPKLACSRCDVIAPLPPKPIERGYASA
GLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSSL
PAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRK
ARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEP
EDWLREVIEKLNDWPSNQVHELLPWNFSSVK

Blast result :

Comments

ORF1, ORF2 and ORF3 of ISSfl3 are respectively 57%, 86%, and 71% aa similar to ORF1, ORF2 and ORF3 of ISCro1.

The ORF2 and ORF3 are reconstructed in silico because there are ponctual mutations at position 965 and 1656.

References

1] Parsot. (1999) Direct submission.
2] Buchrieser,C., Glaser,P., Rusniok,C., Nedjari,H., D'Hauteville,H., Kunst,F., Sansonetti,P. and Parsot,C.(2000) Mol. Microbiol. 38 (4), 760-771
3] Glaser,P., Buchrieser,C., Rusniok,C., Nedjari,H., d'Hauteville,H., Kunst,F., Sansonetti,P. and Parsot,C. (2000) Direct submission GenBank.