ISS1B
- Family IS6
- Group
Isoform ISS1SSynonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| D63820 | ND | Lactococcus lactis | Lactococcus lactis subsp. lactis biovar diacetylactis B-1 plasmid pTL02 |
DNA section
IS Length : 808 bp
Ends
IR Length : 18
IRL : GGTTCTGTTGCAAAGTTTTCTGATAAGTCTATTTTAGTGTAAAATGAATA
IRR : GGTTCTGTTGCAAAGTTTAAAAATCAAATACAAGGTTTATAATCCTTCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGTTCTGTTGCAAAGTTTTCTGATAAGTCTATTTTAGTGTAAAATGAATAAAAATGACAGCGAGGATATATCAATGAACTATTTTAAAGGTAAACAATTT
CAAAACGATGTGATTATTGTCGCTGTTGGTTATTATCTACGTTACAATCTAAGCTATCGTGAAATTCAGGAACTCCTTTATGATCGTGGAATTAACGTTT
GTCACACAACTATTTATCGTTGGGTACAAGAATACAGTAAAGTCCTCTATCATCTCTGGAAAACGAAAAATAGACAGTCCTTCTATTCGTGGAAAATGGA
TGAAACTTATATCAAAATCAAAGGTCGTTGGCATTATCTCTATCGTGCAATTGATGCGGATGGATTGACTTTAGACATCTGGCTACGCAAGAAACGGGAT
ACTCAAGCTGCTTATGCGTTCTTGAAACGACTACATAAACAGTTTGGTCAACCAAGAGTAATTGTCACGGATAAAGCGCCCTCTATTGGTTCTGCATTTA
GAAAGTTACAGAGTAACGGTTTATATACTAAGACAGAGCATCGAACCGTGAAGTATCTCAATAACCTCATTGAGCAAGACCATCGACCAATCAAACGACG
CAATAAATTTTATCGAAGTCTACGAACTGCCTCAACCACGATTAAGGGCATGGAAACAATTCGAGGAATATACAAAAAGAACCGAAGAAATGGAACGCTC
TTCGGATTTTCGGTATCTACTGAGATTAAGGTCTTAATGGGAATATTAGCTTAAGAACAAGAAGGATTATAAACCTTGTATTTGATTTTTAAACTTTGCA
ACAGAACC
CAAAACGATGTGATTATTGTCGCTGTTGGTTATTATCTACGTTACAATCTAAGCTATCGTGAAATTCAGGAACTCCTTTATGATCGTGGAATTAACGTTT
GTCACACAACTATTTATCGTTGGGTACAAGAATACAGTAAAGTCCTCTATCATCTCTGGAAAACGAAAAATAGACAGTCCTTCTATTCGTGGAAAATGGA
TGAAACTTATATCAAAATCAAAGGTCGTTGGCATTATCTCTATCGTGCAATTGATGCGGATGGATTGACTTTAGACATCTGGCTACGCAAGAAACGGGAT
ACTCAAGCTGCTTATGCGTTCTTGAAACGACTACATAAACAGTTTGGTCAACCAAGAGTAATTGTCACGGATAAAGCGCCCTCTATTGGTTCTGCATTTA
GAAAGTTACAGAGTAACGGTTTATATACTAAGACAGAGCATCGAACCGTGAAGTATCTCAATAACCTCATTGAGCAAGACCATCGACCAATCAAACGACG
CAATAAATTTTATCGAAGTCTACGAACTGCCTCAACCACGATTAAGGGCATGGAAACAATTCGAGGAATATACAAAAAGAACCGAAGAAATGGAACGCTC
TTCGGATTTTCGGTATCTACTGAGATTAAGGTCTTAATGGGAATATTAGCTTAAGAACAAGAAGGATTATAAACCTTGTATTTGATTTTTAAACTTTGCA
ACAGAACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 681 bp | 226 aa | 74 | 754 | + | No |
Chemistry : DDE
ORF sequence :
MNYFKGKQFQNDVIIVAVGYYLRYNLSYREIQELLYDRGINVCHTTIYRWVQEYSKVLYHLWKTKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTL
DIWLRKKRDTQAAYAFLKRLHKQFGQPRVIVTDKAPSIGSAFRKLQSNGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIY
KKNRRNGTLFGFSVSTEIKVLMGILA
DIWLRKKRDTQAAYAFLKRLHKQFGQPRVIVTDKAPSIGSAFRKLQSNGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIY
KKNRRNGTLFGFSVSTEIKVLMGILA
Blast result :
Comments
ISS1B transposase is 98 % identical to that of IS946V. ISS1B is located on the lactose-citrate plasmid pTL02 (Sawaguchi et al., 1995).
References
1] Sawaguchi, T., Motoshima, H., Minagawa, E., and Tsukasaki, F. (1995) "Isolation and analysis of lactose-citrate plasmid pTL02 derived from Lactococcus lactis subsp. lactis biovar. diacetylactis B-1" EMBL Data Library, Rel. 45.