ISfinder Mirror Site

ISRso11

Family IS3
Group IS150

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_003295	ND	Ralstonia solanacearum	Ralstonia solanacearum

DNA section

IS Length : 1449 bp

Ends

IR Length : 20/24

IRL : TGAACTGACCCCGTAAAGTTGGACGGGTAATTTAGGCGGCCAAGGGCTGG
IRR : TGAACTGCACCCCAAAAGTTGGACATCTGTTCAACCTTTGGGGTGTTTTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAAGGCCGCT	CCG	ACGTCAATCG	3
GATTGACGTC	GGA	GCGGCCTTTT	3

DNA sequence

TGAACTGACCCCGTAAAGTTGGACGGGTAATTTAGGCGGCCAAGGGCTGGGTCCTGTATTGCACAGGGCTCAGCCCTTTTAGCTTGATCTTGATGCGCTC
GTGATTGTAGTACCGGATGTATTCGGCAATGGCGCGTCGCAGTTGGTCGATGTCCGCGAAGCGCTCTAACCGGAAGCACTCTGACTTGAGTGTGCCGAAG
AAGCTTTCCATTGCCGCATTGTCTAGGCAGTTGCCTTTGCGGGACATGCTCTGGCGCAGGCCTCGCGCGGCCAGTTTGCGACGATACGCTGCCATTTGAT
ACGGCCAGCCTTGATCCGAATGCAGCATCGGCGTCTCGCACGGGCGCAGCTTGCGCAACGCCTTGTCGAGCATCTCCCGGATCAGCGTGTAATCAGGTCG
TTCACTGGTTTGCCAGGCGACGATCTCTCCGTTGTATAGGTCCAGGACGGGCGACAGGTACAGCTTTTTGCCGCCGACGTTGAACTCCGTCACGTCCGTT
ACCCACTTCTCGTTCGGCTTGCTCGCCTCAAACTCGCGCTGTAGCAGATTGGGAGCAACCCGCCCGACTTGCCCGAGGTAAGAGCGGTATTTCTTCGGCC
GCACGAGTGACTTCAACTGTAGCGCCGACATCAATCGCTGTACGGTCTTGTGATTCACCAACGTCCCTGCCCGACGGAGCGTCGCCGTGATCCGGCGGTA
GCCATATCGGCCCTTGTGCTGGGCATAGATGTGCTGAATGCGCTCCTTGAGCGCGCCGTGACGATCCTCTGCCTGGCCTGTCGCCATCTGGTAGTAGTAC
GTGCTGCGTGCGAGATTCGCGGCCTTGAGCAAGCTCGACAATGAATGCCGCTCACGCAGCGCACTCACGACTTGCGCTTTTTCTTTGGTGCCTGCTGCTG
TTTGGCACGCAGCAGGGCATCTAACTTTTTTAGGTACGCCACCTCCGCGCGCAAACACTCGTTCTCTTTGAGCAAATCCTCGCGCGAGCGCTCGTCTTGA
GCCGGCTGCGCAGCGGGTTGGGGATCTTGCTTGGACTGGGACATGATGGGTGCCCCGCCTTTGCGGCGCGGCTGTAGGGCGTCGAAACCGCCCTCATGAT
ACTGGCGGTCCCAGCGGCTGACGCCGCCAGCCTCACGCAAATCGAACACAGCGCTGACTTGCTGATAGGACAACTCGTCGCGCCACATGCGCTGCAGCAC
CGTCAGCTTGAACTCGGCGCTATACGCTTCATGCTTCTTGCGTAGTCCGGATTTCCCATGGCGCTGCCACGCGTTGATCCAGCGCCGGATCACAGAACGG
CCTATCCCGTACTTCTGGCTCAGCCCTGCGGTCCCAACGGCCCCCCTCAAATACTCCTGAACTACTTGGCGCTTGAACGCCTCTTCATACTTCGCCATGA
AAAACACCCCAAAGGTTGAACAGATGTCCAACTTTTGGGGTGCAGTTCA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
534 bp	177 aa	52	585	+	No

ORF function : Transposase
Description :

ORF sequence :

MAKYEEAFKRQVVQEYLRGAVGTAGLSQKYGIGRSVIRRWINAWQRHGKSGLRKKHEAYSAEFKLTVLQRMWRDELSYQQVSAVFDLREAGGVSRWDRQY
HEGGFDALQPRRKGGAPIMSQSKQDPQPAAQPAQDERSREDLLKENECLRAEVAYLKKLDALLRAKQQQAPKKKRKS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
837 bp	278 aa	582	1418	+	No

ORF function : Transposase
Description :

ORF sequence :

MSALRERHSLSSLLKAANLARSTYYYQMATGQAEDRHGALKERIQHIYAQHKGRYGYRRITATLRRAGTLVNHKTVQRLMSALQLKSLVRPKKYRSYLGQ
VGRVAPNLLQREFEASKPNEKWVTDVTEFNVGGKKLYLSPVLDLYNGEIVAWQTSERPDYTLIREMLDKALRKLRPCETPMLHSDQGWPYQMAAYRRKLA
ARGLRQSMSRKGNCLDNAAMESFFGTLKSECFRLERFADIDQLRRAIAEYIRYYNHERIKIKLKGLSPVQYRTQPLAA

Blast result :

Comments

There are 4 copies of ISRso11 on Ralstonia solanacearum genome (4 on chromosome).

References

1] Salanoubat,M., Genin,S., Artiguenave,F., Gouzy,J., Mangenot,S.,Arlat,M., Billault,A., Brottier,P., Camus,J.C., Cattolico,L., Chandler,M., Choisne,N., Claudel-Renard,C., Cunnac,S., Demange,N., Gaspin,C., Lavie,M., Moisan,A., Robert,C., Saurin,W., Schiex,T., Siguier,P., Thebault,P., Whalen,M., Wincker,P., Levy,M., Weissenbach,J. and Boucher,C.A. (2002) Nature 415 (6871), 497-502