ISRm3

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) ISRm-3
Accession numberTranspositionOriginHost
M60971 Y Rhizobium meliloti
Rhizobium meliloti WL131
Rhizobium meliloti 102F70
Rhizobium leguminosarum biovar phaseoli
Rhizobium leguminosarum biovar viciae DMG112
Rhizobium meliloti 102F51
Rhizobium meliloti 2012
Rhizobium meliloti 2035
Rhizobium meliloti ATCC9930
Rhizobium meliloti DMG117
Rhizobium meliloti IZ450 plasmid pTA2
Rhizobium meliloti JJ1c10 plasmid pRmeJJ1c10a
Rhizobium meliloti NRG43
Rhizobium meliloti RCR2011 plasmid pSym
Rhizobium meliloti SU47 plasmid pRmeSU47a
Rhizobium meliloti U45
DNA section
IS Length : 1298 bp

Ends


IR Length : 30

IRL : GGGACTGTCAGGAATTCTGTGCGGTGGGCCATGATGATGAAAAGGAGAGA
IRR : GGGACTGTCAGGAATTCTGTGCGGTGGGCCGGTTTCATCAGTTGAAGAAC

Insertion site


Left flankDirect repeatRight flankDR Length
CTCACGGCCGATTGAGGGCG8
CGTTCTGCCGCGTCTTTCGG8
ACATGTTGTTCTTGACGATGC9
GCGCGGATAGCGGACCGACCA9
CCGGAAGCTGCCTGGCAGCGC9

DNA sequence

GGGACTGTCAGGAATTCTGTGCGGTGGGCCATGATGATGAAAAGGAGAGAATCATCACATGGCTATCGAGAAAGAACTTCTGGACCAGCTCCTGGCTGGA
CGTGATCCATCCGAGGTTTTCGGCAAGGACGGTTTGCTGGACGATCTGAAGAAGGCGCTTTCAGAGCGCATCCTCAATGCGGAGCTTGACGACCATCTCG
ACGTCGAGCGCCTGGAGGGCGGCCCCGCCAACAGGCGCAACGGTTCCTCCAAGAAGACGGTTTTGACTGGCACATCGAAGATGACGCTGACCATCCCGCG
CGATCGGGCGGGTACCTTCGACCCAAAGCTGATCGCCAGATATCAGCGCCGGTTTCCCGATTTCGACGATAAGATCATTTCGATGTACGCCCGTGGTATG
ACAGTGCGCGAGATCCAGGGGCATCTTGAAGAGCTCTACGGCATCGATGTGTCGCCGGATCTGATCTCGGCGGTGACCGATACGGTTCTGGAGGCCGTTG
GAGAGTGGCAAAACCGGCCGCTCGAGCTTTGCTACCCCCTCGTGTTTTTCGACGCCATCCGGGTCAAGATCAGAGACGAGGGCTTCGTACGCAACAAAGC
CGTCTATGTCGCCCTGGCCGTGCTCGCTGACGGCAGCAAGGAGATCCTCGGGCTCTGGATCGAGCAGACGGAAGGGGCAAAGTTCTGGCTGCGGGTCATG
AACGAGCTGAAGAACCGCGGTTGCCAGGATATCCTAATCGCCGTGGTCGACGGCTTGAAGGGCTTCCCCGAGGCCATCACCGCCGTCTTTCCCCAAACAA
TCGTCCAGACCTGCATCGTCCACCTGATCCGGCACTCGTTGGAGTTCGTATCCTACAAGGATAGAAGGACCGTTGTGCCGGCGTTGAGAGCCATCTACCG
CGCCCGAGATGCCGAGGCGGGCCTGAAGGCGCTGGAGGCCTTCGAGGAAGGGTACTGGGGCCAGAAATATCCCGCTATCGCTCAAAGCTGGCGGCGCAAC
TGGGAACACGTCGTTCCCTTCTTCGCCTTCCCCGAAGGGGTCCGCCGCATCATCTACACGACGAACGCAATAGAGGCCCTCAACTCGAAGCTTCGGCGAG
CTGTGCGTTCCCGCGGGCATTTCCCTGGTGACGAAGCCGCGATGAAGCTGTTATATCTCGTTCTTAACAACGCGGCCGAGCAATGGAAACGGGCGCCGCG
GGAATGGGTCGAGGCAAAGACACAGTTCGCCGTCATCTTTGGCGAGCGGTTCTTCAACTGATGAAACCGGCCCACCGCACAGAATTCCTGACAGTCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1203 bp400 aa591261+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAIEKELLDQLLAGRDPSEVFGKDGLLDDLKKALSERILNAELDDHLDVERLEGGPANRRNGSSKKTVLTGTSKMTLTIPRDRAGTFDPKLIARYQRRFP
DFDDKIISMYARGMTVREIQGHLEELYGIDVSPDLISAVTDTVLEAVGEWQNRPLELCYPLVFFDAIRVKIRDEGFVRNKAVYVALAVLADGSKEILGLW
IEQTEGAKFWLRVMNELKNRGCQDILIAVVDGLKGFPEAITAVFPQTIVQTCIVHLIRHSLEFVSYKDRRTVVPALRAIYRARDAEAGLKALEAFEEGYW
GQKYPAIAQSWRRNWEHVVPFFAFPEGVRRIIYTTNAIEALNSKLRRAVRSRGHFPGDEAAMKLLYLVLNNAAEQWKRAPREWVEAKTQFAVIFGERFFN

 

Blast result :
Comments
ISRm3 has been found inserted in the middle of ISRm5. ISRm3 is present in 60/61 isolates of Rhizobium meliloti, with a copy number varying between 1 and 11 (Barran et al., 1994). There is also a good correlation between ISRm3-generated hybridization profiles and phage typing.
References
1] Renalier, M.-H., Batut, J., Ghai, J., Terzaghi, B., Gherardi, M., David, M., Garnerone, A.-M., Vasse, J., Truchet, G., Huguet, T., and Boistard, P. (1987) J. Bacteriol. 169, 2231-2238.
2] Wheatcroft, R., and Laberge, S. (1991) J. Bacteriol. 173, 2530-2538.
3] Ogawa, J., Brierley, H.L., and Long, S.R. (1991) J. Bacteriol. 173, 3060-3065.
4] Soto, M.J., Zorzano, A., Olivares, J., and Toro, N. (1992) Plant Mol. Biol. 20, 307- 309.
5] Barran, L.R., Bromfield, E.S.P., Laberge, S., and Weatcroft, R. (1994) Mol. Ecol. 3, 267-270.