ISfinder Mirror Site

ISRm14

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AF134706	Y	Sinorhizobium meliloti	Sinorhizobium meliloti

DNA section

IS Length : 2695 bp

Ends

IR Length : 18/22

IRL : GTAAGCGCCGTCTCCGCCCCATTGGATAGGCTGTATTTTGAGGCTGGCTG
IRR : GTATGCGGCGTCCACGCCCCATCGATTATTCAGTTGCGGCGATCTGAGGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length

DNA sequence

GTAAGCGCCGTCTCCGCCCCATTGGATAGGCTGTATTTTGAGGCTGGCTGGGCGTGCGAGAAGGTTTCCAGGACTATCTGGAGATTTGCATGGCAGATGA
TGGATTTGTTGGGCGCTACGAAGTTGTCGAGCCGCGCCGCGGAAACCGGCGTTGGCCCGATGATGTGAAGGCGCGGATTGTCGCGGAGAGCCTTGAGCCG
GGTGTTCGAGTTGTTGATGTCGCGCGCCGTCATGACGTTGTTCCGCACCAGCTTTCCTTCTGGCGTCGACAAGCGCGCGAGGGCATTCTGGCGCTGCCGT
TTGAGGCTATGCCGGGCCTGTCGGAGAGCGGCGATGCCGAGCCTGCATTTGTGCCTTTGGCGATTGCGGCAGAGCCGAGCGAGGCTGTGAATGTTTTGGC
GCCGCCGCTGTCGGAGGCGGTTTCGTCGGTCTTGACGTTGGAGATCGGCCCGGACGTTGTGTTGCGGGTTCCCGGCGATGTGCCGGTTGAACGTGTGGCG
GCTCTGGTGCGAGCCATGCGAGCGCCGGTATGATCGTCGCGGGCCAACGACTGCCGATCCTGATTGCAACGCGGCCGGTGGACTTCCGCTGTGGGCATCA
GGCGCTGGCTCTGATGGTGCAGACCGAGTTGAAGCTCGACCCGCATTCCGGGGTGACGGTGATCTTCCGGTCGAAGCGCGGTGATCGCCTGAAAATCCTG
GTGTGGGATGGCACCGGAATGGTGCTAACCTACAAAATTCTTGAACATGGAAGCTTTGCCTGGCCCAAGGTGCAGGATGGGACGATGCGTCTTTCCAGGG
GTCAATATGAGGCTTTGTTCGAAGGTCTTGACTGGCGACGGGTGATGGCGCAACGGGTGACGGCGCCGTCGGCGGCAGGGTGAGTATCCGGCCGTCTTTG
CATTGTTTTATTTGGCTTTTTTGTGCTGCCTTGCTATAAGGCCGCATGTCGTCTCCCCTTGATCTCAGCCTGTTTCCGAACCTTCCGCCAGAGGTGGTGA
AGGCGTTTGCGGCGATGCAGTTCGAGCTGTCGGTCGAGCGTGCTGCGCGTCAGCATGAGCAGGCTGTGGTGGCCGAAAAGGACGCGTTCATCGCCGAGTT
GAAGGAACTGATCGAGAAGCTTGAGGGGCAGGTTCACGACTATCGGCGCACGAAGTTCGGGCCGAAATCGGAAAAGCTCGATCCGGCGCAGATGGAACTG
GCGCTGGAAGACCTTGAAACGGCAATTGCCGAAACACAGGCGCGGATTGCCGCCGTCGAGAAAAAGATCGAAGCCAGCGCATCTGATCCGGAAAAAGTCG
CTCCTCGCAAGGAGCGTAAGGCCCGTGCACTGCCCGAACATTTGCCGCGGGTCGAGCGGGTGATCGAGCCTGAGAGCATCGTTTGTCCCTGCGGTTGCGG
CAACATGGTCCGGATCGGCGAAGACCGGACGGAACGGCTCGACCGGATTCCGGCGCGCTACGAGGTGATCGTCACGATCCGCCCGAAATACGCCTGCCCC
AAGGGTCGAACGGGCGTCGTCCAGGCCAGAGCGCCGGCGCATCTCTTGGAAGGGAGCTGGCCGACCGAAGCCCTTCTGGCTGAGATTGCCGTCTCCAAGC
ATTCCGAACATATGCCGCTCAACCGGCAGGCCGAGGTCATGGCGCGACACGGGGTGCCGATAGACCGCACCGTCCTGGCCGATTGGATGGGCAGGACGGG
TGCTGCGATCGCGCCGGTGGTCGACCATATGGCCAAACGGCTGCTGTGGGAAAGCACGCGGCTTTATGTCGACGAGACAACGGCTCCGGTGCTTGATCCG
GGGCGAGGCAAAACGAAGACCGGTTATCTATGGGCCGTGTTGCGTGACGATCGCGGCTGGAATGGCTCTGCGCCGCCAGGCGTGGTGTTCCATTACCGGC
CCGGGCGTAAAGGCGAATATGCCGCTGAAATCCTCGACGGGTTCAACGGGACAATCCAGGTGGATGCCTACGGTGGTTACTCTCACCTCGCCACGTTGGA
CCGGGTGGGTGGCGATCCCTTGAAGCTGGCTTTCTGTTGGGCGCACGGGCGCAGAAAGCTGATCAAAGCCACGCCAAAGAGTGGATCGCCCATCGTCGAC
GAGGCGCTGGTGCGGATCGCCGCGCTCTACAAGATCGAAGACAGTATCCGTGGCTCAGATCCCGAACATCGCCGGGCAGTTCGACAGGACCTCTCCCTCC
CGCTGGTGGACGCGTTCTTCGCCTGGCTGGCAGCGCAAGCCAAGCGCGTCTCACGCAAGTCTGACCTCGGAAAAGCCCTGGCCTATATGCTAACGCGGCA
GGACGGGTTCCGGCTGTTCCTGGACGACGGCCACGTCGATATCGACTCCAACCTGGTGGAAAACGCGATCCGCCGACCGGCCATGAACCGCCGCAATGCG
CTCTTTGCGGGGCACGATGAAGGGGGCCGCAATTGGGCCCGGTTTGCCAGCCTGATCGGCACTTGTAAAATGAACGGCGTTGAGCCCTACGCCTATCTGT
GCAACCTCTTCACCCGCCTCGCAAACGGCCACCTCGCCAAAGACATCGATGCCCTGATGCCATGGGCCTATGCCGCTCGCATCCAGGCCTCACAATGAGC
TCGTCAGATACTCTTCGGTGAGCTCATTTGACGGCCCGTCGATCAACCTCAGATCGCCGCAACTGAATAATCGATGGGGCGTGGACGCCGCATAC

Protein section

ORF number : 5

ORF 1

Length		Begin	End	Strand	Fusion ORF
444 bp	147 aa	90	533	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MADDGFVGRYEVVEPRRGNRRWPDDVKARIVAESLEPGVRVVDVARRHDVVPHQLSFWRRQAREGILALPFEAMPGLSESGDAEPAFVPLAIAAEPSEAV
NVLAPPLSEAVSSVLTLEIGPDVVLRVPGDVPVERVAALVRAMRAPV

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
354 bp	117 aa	530	883	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIVAGQRLPILIATRPVDFRCGHQALALMVQTELKLDPHSGVTVIFRSKRGDRLKILVWDGTGMVLTYKILEHGSFAWPKVQDGTMRLSRGQYEALFEGL
DWRRVMAQRVTAPSAAG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1653 bp	550 aa	946	2598	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSSPLDLSLFPNLPPEVVKAFAAMQFELSVERAARQHEQAVVAEKDAFIAELKELIEKLEGQVHDYRRTKFGPKSEKLDPAQMELALEDLETAIAETQAR
IAAVEKKIEASASDPEKVAPRKERKARALPEHLPRVERVIEPESIVCPCGCGNMVRIGEDRTERLDRIPARYEVIVTIRPKYACPKGRTGVVQARAPAHL
LEGSWPTEALLAEIAVSKHSEHMPLNRQAEVMARHGVPIDRTVLADWMGRTGAAIAPVVDHMAKRLLWESTRLYVDETTAPVLDPGRGKTKTGYLWAVLR
DDRGWNGSAPPGVVFHYRPGRKGEYAAEILDGFNGTIQVDAYGGYSHLATLDRVGGDPLKLAFCWAHGRRKLIKATPKSGSPIVDEALVRIAALYKIEDS
IRGSDPEHRRAVRQDLSLPLVDAFFAWLAAQAKRVSRKSDLGKALAYMLTRQDGFRLFLDDGHVDIDSNLVENAIRRPAMNRRNALFAGHDEGGRNWARF
ASLIGTCKMNGVEPYAYLCNLFTRLANGHLAKDIDALMPWAYAARIQASQ

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
417 bp	138 aa	2049	2465	-	No

ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MRVELQLGLHHQSQRLMPTAEVHRPRCNQDRQSLARDDHTGARMARTRAATRSTGTSPGTRNTTSGPISNVKTDETASDSGGAKTFTASLGSAAIAKGTN
AGSASPLSDRPGIASNGSARMPSRACRRQKESWCGTTS

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
795 bp	264 aa	967	1761	-	No

ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MVDHRRDRSTRPAHPIGQDGAVYRHPVSRHDLGLPVERHMFGMLGDGNLSQKGFGRPAPFQEMRRRSGLDDARSTLGAGVFRADRDDHLVARRNPVEPFR
PVFADPDHVAATAGTNDALRLDHPLDPRQMFGQCTGLTLLARSDFFRIRCAGFDLFLDGGNPRLCFGNCRFKVFQRQFHLRRIELFRFRPELRAPIVVNL
PLKLLDQFLQLGDERVLFGHHSLLMLTRSTLDRQLELHRRKRLHHLWRKVRKQAEIKGRRHAAL

Blast result :

Comments

ORFs 4 and 5 are on the complementary strand. ISRm14 has been chosen as the family type. It is present with a copy number from 1 to 6 in 66% of S. meliloti strains tested. (Schneiker et al., 1999) and is closely related to ISRLdTAL1145-1.

References

1] Simon,R., Hotte,B., Klauke,B. and Kosier,B.(1991) J. Bacteriol. 173 (4), 1502-1508.Schneiker,S., Kosier,B., Puehler,A. and Selbitschka,W.(1999) Curr.Microbiol. 39, 274-281.