ISRm12
- Family IS5
- Group IS5
Isoform Synonym(s) ISRm102F34-1
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF087641 | ND | Sinorhizobium meliloti | Sinorhizobium meliloti |
DNA section
IS Length : 1481 bp
Ends
IR Length : 15
IRL : GTCGGCGCTGAAAAATCTCTCAACGCCTTCGATTAAGGGATTCCCATCTG
IRR : GTCGGCCCTGAACAAAGGCTAGATCATTGATCTTCTTGCGGTTGGACGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GTCGGCGCTGAAAAATCTCTCAACGCCTTCGATTAAGGGATTCCCATCTGTTTTGAAGTATTGGAAACTGCCGGAAATCGTCGGTGATGTAGCTGATTTC
TGGTGTTTTTGGTATGCGGCCAAGTCGTCCCTCTTTCGGTAAGAATGATCTTTTTCGCGACCGTCTGGACGCGATCATCAACCCGCGCCAGGCGCTGGTG
CGATTGGCCGGTGTGATCCCATGGGCACGATTTGACGAGGCGTTCGGTGGGTTCTATCGGCCGGTTGGCCGGCCCGCCAAGCCGACGCGGCTGATGGTCG
GGCTGCATTACCTGAAGCATGTCCACGACCTGTCGGATGAGGAGGTGGTCGAGCGCTGGGTGGAGAATCCGTATTGGCAGTTTTTCTGCGGCTTTGAGTT
CTTCCAGCATGAAGCGCCGATCGACGCGAGCACGATGACGCCTTGGCGCAAGCGGATCGGGCCGCAGGGGCTGGAGGAATTGCTGAAGGCCAGCGTCGAG
GCGGCGCTTCAGACGGGAACGGCCAGGCCGGAATCTTTGGAGCGGGTCAATGTCGACACGACGGTGCAGCCGAAAGCCATCGCTCATCCGACCGACAGCA
GGCTGTATCTGAAGGCTCTGCAGATGCTGGTCCGTCACGCCAAGAAGCATGGCATCGAGCTGAGGCAGAGCTACACGCGTCTGGCGAAAGCAGCGGCGGT
GCGCGCCGGCCGTTATGCCCATGCCCGGCAATTCCGCCGCATGCGACGCGAACTGAAGCGCCTGCGCACCTTTCTTGGCCGCGTCTTGCGCGATATCGGC
CGCAAGATCGCCGGCAAGGTCGAACTGGAGAGGACTTTCGCCCGGCTGTTCGGTCTTGTCGAACGGCTGCTGGCGCAAAAGCCGAAGGACAAGAACAAGC
TCTATTCGCTGCATGCTCCCGAGGTCGTCTGCATCTCGAAGGGCAAGGCTCGCACGCCATACGAGTTCGGTTGCAAGGTAGGGATCGCGGCGACGAACCG
CGAAGGGCTGGTTCTGGCGGCCAAGGCGTTCGAAGACAATCCCTATGACGGGCACACCCTCTCACGCACCGTCGATCAGGCTGTTGCCATGGGTGGTATC
GATCCGGATCGCATCTACGTCGATAAGGGCTATCGCGGTCATGATTACACCGGGTCGGCGTCGGTGATGATTGCGGAAGCCGGCGCGGTGACGCCGACCA
TGCGACGGGAGCTGAAACGACGATCGGCCATCGAGCCCACCATCGGCCATATGAAGACCGATGGCAGGCTGGACCGGAACTTCCTTCTCGGGCACGATGG
CGACACCGTCAATGCGCTGCTCGTGGCCGCAGGTCACAATCTGCGCCTCATCCTGGCCAAACTGGCCCTTTGGCGTGCCTTCATCAGGGCCGCGCTTACC
GTTCTCATGGCGAAATCGGATACTTCTCCAGATCGTCCAACCGCAAGAAGATCAATGATCTAGCCTTTGTTCAGGGCCGAC
TGGTGTTTTTGGTATGCGGCCAAGTCGTCCCTCTTTCGGTAAGAATGATCTTTTTCGCGACCGTCTGGACGCGATCATCAACCCGCGCCAGGCGCTGGTG
CGATTGGCCGGTGTGATCCCATGGGCACGATTTGACGAGGCGTTCGGTGGGTTCTATCGGCCGGTTGGCCGGCCCGCCAAGCCGACGCGGCTGATGGTCG
GGCTGCATTACCTGAAGCATGTCCACGACCTGTCGGATGAGGAGGTGGTCGAGCGCTGGGTGGAGAATCCGTATTGGCAGTTTTTCTGCGGCTTTGAGTT
CTTCCAGCATGAAGCGCCGATCGACGCGAGCACGATGACGCCTTGGCGCAAGCGGATCGGGCCGCAGGGGCTGGAGGAATTGCTGAAGGCCAGCGTCGAG
GCGGCGCTTCAGACGGGAACGGCCAGGCCGGAATCTTTGGAGCGGGTCAATGTCGACACGACGGTGCAGCCGAAAGCCATCGCTCATCCGACCGACAGCA
GGCTGTATCTGAAGGCTCTGCAGATGCTGGTCCGTCACGCCAAGAAGCATGGCATCGAGCTGAGGCAGAGCTACACGCGTCTGGCGAAAGCAGCGGCGGT
GCGCGCCGGCCGTTATGCCCATGCCCGGCAATTCCGCCGCATGCGACGCGAACTGAAGCGCCTGCGCACCTTTCTTGGCCGCGTCTTGCGCGATATCGGC
CGCAAGATCGCCGGCAAGGTCGAACTGGAGAGGACTTTCGCCCGGCTGTTCGGTCTTGTCGAACGGCTGCTGGCGCAAAAGCCGAAGGACAAGAACAAGC
TCTATTCGCTGCATGCTCCCGAGGTCGTCTGCATCTCGAAGGGCAAGGCTCGCACGCCATACGAGTTCGGTTGCAAGGTAGGGATCGCGGCGACGAACCG
CGAAGGGCTGGTTCTGGCGGCCAAGGCGTTCGAAGACAATCCCTATGACGGGCACACCCTCTCACGCACCGTCGATCAGGCTGTTGCCATGGGTGGTATC
GATCCGGATCGCATCTACGTCGATAAGGGCTATCGCGGTCATGATTACACCGGGTCGGCGTCGGTGATGATTGCGGAAGCCGGCGCGGTGACGCCGACCA
TGCGACGGGAGCTGAAACGACGATCGGCCATCGAGCCCACCATCGGCCATATGAAGACCGATGGCAGGCTGGACCGGAACTTCCTTCTCGGGCACGATGG
CGACACCGTCAATGCGCTGCTCGTGGCCGCAGGTCACAATCTGCGCCTCATCCTGGCCAAACTGGCCCTTTGGCGTGCCTTCATCAGGGCCGCGCTTACC
GTTCTCATGGCGAAATCGGATACTTCTCCAGATCGTCCAACCGCAAGAAGATCAATGATCTAGCCTTTGTTCAGGGCCGAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1350 bp | 449 aa | 114 | 1463 | + | No |
Chemistry : DDE
ORF sequence :
MRPSRPSFGKNDLFRDRLDAIINPRQALVRLAGVIPWARFDEAFGGFYRPVGRPAKPTRLMVGLHYLKHVHDLSDEEVVERWVENPYWQFFCGFEFFQHE
APIDASTMTPWRKRIGPQGLEELLKASVEAALQTGTARPESLERVNVDTTVQPKAIAHPTDSRLYLKALQMLVRHAKKHGIELRQSYTRLAKAAAVRAGR
YAHARQFRRMRRELKRLRTFLGRVLRDIGRKIAGKVELERTFARLFGLVERLLAQKPKDKNKLYSLHAPEVVCISKGKARTPYEFGCKVGIAATNREGLV
LAAKAFEDNPYDGHTLSRTVDQAVAMGGIDPDRIYVDKGYRGHDYTGSASVMIAEAGAVTPTMRRELKRRSAIEPTIGHMKTDGRLDRNFLLGHDGDTVN
ALLVAAGHNLRLILAKLALWRAFIRAALTVLMAKSDTSPDRPTARRSMI
APIDASTMTPWRKRIGPQGLEELLKASVEAALQTGTARPESLERVNVDTTVQPKAIAHPTDSRLYLKALQMLVRHAKKHGIELRQSYTRLAKAAAVRAGR
YAHARQFRRMRRELKRLRTFLGRVLRDIGRKIAGKVELERTFARLFGLVERLLAQKPKDKNKLYSLHAPEVVCISKGKARTPYEFGCKVGIAATNREGLV
LAAKAFEDNPYDGHTLSRTVDQAVAMGGIDPDRIYVDKGYRGHDYTGSASVMIAEAGAVTPTMRRELKRRSAIEPTIGHMKTDGRLDRNFLLGHDGDTVN
ALLVAAGHNLRLILAKLALWRAFIRAALTVLMAKSDTSPDRPTARRSMI
Blast result :
Comments
Our analysis showed that it is a member of IS5 subgroup of the IS5 family, closely IS52 (44% identical + similar) and IS1478 (56% identical + similar),
References
1] Selbitschka, W., Zekri, S., Schoeder, G., Puehler, A. and Toro, N. (1999) FEMS Microbiol. Lett. 172, 1-7.
2] Selbitschka, W., Zekri, S., Schoeder, G., Puehler, A. and Toro, N. Direct submission.
2] Selbitschka, W., Zekri, S., Schoeder, G., Puehler, A. and Toro, N. Direct submission.