ISfinder Mirror Site

ISRl1

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISRle3

Accession number	Transposition	Origin	Host
L19650	ND	Rhizobium leguminosarum	Rhizobium leguminosarum biovar viciae 897

DNA section

IS Length : 2495 bp

Ends

IR Length : 14/15

IRL : GTAAGCGACAAGCTGACCCCATCTGGCGTCGGTAGCGTTGGCCGCTGTAT
IRR : GTAAGCGACAAGCCGGGGCCGTTCAGGCGGCGTAGTTCCATGGCATGAGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length

DNA sequence

GTAAGCGACAAGCTGACCCCATCTGGCGTCGGTAGCGTTGGCCGCTGTATTGCGGACAGACGATTCACAAACTGTGAGCTTCTATGTCTGCGCCTAGCTC
TGGAACTAGAAACTTCCATATGATTGAGGCTGTTCCGGAACGGCTTGAGGGCGCCCCACGGCAATTTCGGCGCCGCTGGTCCGATGATTTTAAAGCGCAG
GCTGTGGCTGAGGCAATGGAGCCCGGCGCAAGCGTCTCGGCCATTGCGCATCGCATCGGCATACATCCCTCGCAGCTATTTGGCTGGCGTCGTGATGCTC
GTGACGGACAACGATCCTTCTCGCAAGATCGGGCTGGTCAGACAGGGACCAGGACGATTGGCACACGTGCGATGATCGAGCTTGTGATCGGCGACGTCAT
TATCCGTGCCGACGCAGATATCGGCGAAGCACAGCTGCAGCGGGTGATCCGAGCGGTGCGGTCGGCATGATCCCGTCCGGCGTGAAGGTCTTTCTTGCGA
GCCATCCCATCGACTTCCGAAAGGGGCCGACAGCTTGCTTTCGCTGGTACGGGATGCTGGCAGTGATCGTTCAACGGTGCACTTTATGTCTTTCGGGCCA
AGAGAGCAGACCGAGTCAAGATCGTGTGGTGGGATGGCTCGGCGTTTGCCTCTATGCGAAGCGGCTGGAAAAAGCCCAGTTCTGCTGGCCCCGGATGGCC
ATCATCGCGTCCAGCTCAATCATGCCCAGCTTCTGGCTTTGGTCGACGGAATGGACTGGAAGCGAGTTCGATCCGCGCCGGTGAAGCCTCCCGAGATTGT
TGGGTAAAACCGCTGCGGCGAAGTGAATCAGGTCCCTGAAAGCATGGGCGGAGCGCGGCAAAATATGCTCTGATCATACCCATGACGCGGCCCGAGATTG
AGCTCCCGGATGATGTAGAGGCTCTCAAAGCCATGGTTCTTGCCACTGTGCAAAAGCAGCCCGCGTCGAGGCTTTGGAGAAACAGGTTGACGATCTAGAG
GCCGCAACGCGGACGCCGATGAGCGCATCGAGCGGCTGACGCAAGATCCTGAAGGCTTTCGATCGCGCCCGATTTGGCCGACGCTCGGAAAGGCTTGCAT
CATCCACCGGTCGATGACGAGCAGCATGCCTTTGTCTTCGAAGAGATCGAGACCGGCATTGCGACGATCAAGGCTCAGGTCACGAAAGGCCGCGGTAGCG
CGGACAGCAAACGCGCTCCGCGGCCACGCAAGGGCTTTGCGCCTCATCTTGAGCGCGTCGAGGTCGTGATCGAACCGGAAGAGCTTCCAGAACATGCAGG
TAAGACCAAGATCCTGATTGGCGAAGACGTCTCCGAGCGGCTGGATGTCGTGGCAGCGAAGTTCCGTGTCATCGTCACCCGCCGGCCGAAGTATGCCTTT
AGGAACGAGGATGGTGTCGTCCAGGCGGCGGCTCCGGCACACATCATCGAAGGTGGCATTCCCACGGAAGCACTTCTGGCCCAGATCGCCGTTTCCAAAT
ATGCCGACGGGCTGCCACTCTATCGCCAGGAAGCCATCTACGCACGCGACAAGGTCGAACTCGACGCAAGCTAATGGCTCAATGGATGGGCAAGCTGGGG
TTCGAGCTCAATATCCTCGCCGACTACATCCTCGATGAGATCAAGAAGGCCGAACGGATCTTTGCCGACGAAACGACCTTGCCCACACTTGCTCCTGGTT
CCGGATCAGCGAAGACGGCATGGCTATGGGCGTATGCCAGGGATGACCGACTTTTGGGGGCAGCGGTCCACCGATGGTAGCCTATCGCTTCGAGGACAGT
CGAGCCACCGAGTGTGTCGCACGACACCTGAACGGCTATCGTGGAATCCTGCAGGTCGATGGATATGCCGCTTATAACAAGCTCGTCCGGAAAGATGGAG
GCAATAACAGCGCCATCCTGGCCGGCTGTTGGTCGCACAGCCGGAGAAAGTTCTACGAGTTGCATGTCGCAAAGAGCTCGAAAGTCGCTACCGACACCGT
CGAGCGTATGGCGAGGTTATGGGAGATCGAGGAGCGTGTGCGCGCCCAAAGCCCTGAAGCTCGTGTCGCTGCACGCCAGGAGATCTCCGCAGCGATCGTC
CGTGACCTCTTCACTCTCTGGCAGGCGACCCTGCCGCGGGTCTCTGGAAAATCCAAACCTCGCCGAGGCTCTGCGTTATGCCATCTCGCGTCGAGACATC
TTCGAGCGCTTCCTGACCGACGGCCGCATCGAACTCGACTCCAACATAGTCGAGCGAGCAATCAGACCCCAAGCGATCACGAGAAAAAATAGTCTATTCG
CTGGAAGCGACGGTGGCGGCCGGACTTGGGCGACCATCGCGACGCTCCTGCAAACGGCAAAGATGAATGCCGTCGATCCGCAAGCCTGGTTGACCCAGAC
GCTTGAGGCCCTTGCGAATGGATGGCCCAACAGTGAAATCGACGCCCTCATGCCATGGAACTACGCCGCCTGAACGGCCCCGGCTTGTCGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
387 bp	128 aa	84	470	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSAPSSGTRNFHMIEAVPERLEGAPRQFRRRWSDDFKAQAVAEAMEPGASVSAIAHRIGIHPSQLFGWRRDARDGQRSFSQDRAGQTGTRTIGTRAMIEL
VIGDVIIRADADIGEAQLQRVIRAVRSA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
341 bp	113 aa	467	807	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1592 bp	530 aa	882	2473	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

Blast result :

Comments

Note that the IS292 DNA sequence is 81 % identical to that of ISRl1. However, since there are 18 nt difference between IS292 and ISRl1, randomly distributed, and that IS292 seems to harbor less "errors" than ISRl1, no attempt has been made to fully describe all the ISRl1 ORF.

ISRl1 has 99% DNA identity to ISRle3.
ORF2 and ORF3 were not included due to the presence of multiple mutations which generate frameshifts and stop codons.

References

1] O'Brien, J.K. (1993) Thesis Dublin City University.
2] O'Brien, J.K., Ryan, A.J., and O'Connell, M. (1994) EMBL Data Library, Rel. 36.
3] Ponsonnet, C., Normand, P., Pilate, G., and Nesme, X. (1995) Microbiol. 141, 853-861.