ISfinder Mirror Site

ISRfr1

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
M73698	ND	Rhizobium fredii	Rhizobium fredii USDA205 Rhizobium sp. NGR234 Rhizobium fredii USDA201 Rhizobium fredii USDA257 Rhizobium fredii USDA191 Rhizobium fredii USDA192 Rhizobium fredii USDA193 Rhizobium fredii USDA217

DNA section

IS Length : 1136 bp

Ends

IR Length : 10/17

IRL : GTGGTATCCGCCTAACATAAGAGTCCGTATGGATTCCCTTTGGCGAGTGC
IRR : GTGGATGGAATCTAACACTTGGTGCCCCCGTTTGACGGCAGCGATGATTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
NNNCTGAAGC	TA	GTAAGGGNNN	2

DNA sequence

GTGGTATCCGCCTAACATAAGAGTCCGTATGGATTCCCTTTGGCGAGTGCGTATGCGAGTGTGCCATGCGCACAGGAATAGCCTTCACCGTTTCGCCGAC
CGATCGCCAACGCCTGAGAGCCCTCATCAGTGATCGCAATGCGCCGCAGAAGCACGTCTGGCGCGCCGAGATCATCCTGCTGAGTGCCGATGGCCTCGGC
ACCGTCGAGATCATGCGACAGACCGGCAAGTCGAAGACCTGCGTGTGGCGCTGGAGGACGTCGCCAACGGAAGGCTTCGAGGGTCTCTTGCGGCGACAAG
ACCGGCCCCTCGGCATTGCGCCGCTCGGGCCGGAGATCGGCGAGCAGGTGGTTGCGTTTCGCTTGCCGACCCGCCGGGCGAGACGGACGCACTGGACTGC
CGACATGATGGCGGCCGAGGTGGGCGTCAGCGCCAGCGGTCGCCGCATCTGGAAGGCGCACGGTCTCCAGCCCCACCGCTGGCGGGCCTTCAAGCTCTCC
AACGACCCGCAGTTCGTCGCCAAGCTCAAAGACGTTGTCGGCCTCTACGTCGACCCGCCGGCCCATGCCATCGTGCTGTCGGTCGACGAGAAGAGCCAGA
TCCAGGCGCTCGACCGCACCCAGCCCGGCCTGCCCCTGAAGAAGGGCCGGCTCGGCACCATGACCCACGACTACAAGCGGCATGGCACGACCACGTTGTT
TGCCGCCCTCAACGTGCTCGACGGCACCGTCATCGGCAGGAACATGCAGCGTCACAGGCATCAGGAGTTCATCCGTTTTCTCAACGCCATCAACGCCCAA
GTGCCGGCCGACAAGGCGATCCACGTCATCCTCGACAACTATGCCGCCCACAAGCATCCCAAGGTGCGCGCCTGGCTCGACCGCCATCAGCGCTTTACCT
TCCACTTCACGCCGACGTCCTGCTCGAGGCTCAACGCCGTCGAGGGCTTCTTCGCCAAACTGTCGAAGCGTCGCCTCAAGCGCGGCGTCTTTCATTCGGT
CGTTGACCTCCAGGCCGCCATCAACCGCTTCCTTACAGAGCACAACCAACAACCCAAGCCCTTCACCTGGACCGCCGATCCCGACAAAATCATCGCTGCC
GTCAAACGGGGGCACCAAGTGTTAGATTCCATCCAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1074 bp	357 aa	66	1139	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRTGIAFTVSPTDRQRLRALISDRNAPQKHVWRAEIILLSADGLGTVEIMRQTGKSKTCVWRWRTSPTEGFEGLLRRQDRPLGIAPLGPEIGEQVVAFRL
PTRRARRTHWTADMMAAEVGVSASGRRIWKAHGLQPHRWRAFKLSNDPQFVAKLKDVVGLYVDPPAHAIVLSVDEKSQIQALDRTQPGLPLKKGRLGTMT
HDYKRHGTTTLFAALNVLDGTVIGRNMQRHRHQEFIRFLNAINAQVPADKAIHVILDNYAAHKHPKVRAWLDRHQRFTFHFTPTSCSRLNAVEGFFAKLS
KRRLKRGVFHSVVDLQAAINRFLTEHNQQPKPFTWTADPDKIIAAVKRGHQVLDSI

Blast result :

Comments

ISRfr1 lies 1 kb upstream of the nodABC genes. ISRf1 ORF is 74 % identical to that of ISAr1. The ORF stop codon is the TAG of the CTAG target site. For internal consistency, duplication of two bp is proposed here, although duplications of 0, 2 or 4 bp are formally possible too.

References

1] Krishman, H.B., and Pueppke, S.G. (1991) Mol. Plant Microbe Interact. 4, 521-529.
2] Fournier, P., Paulus, F., and Otten, L. (1993) J. Bacteriol. 175, 3151-3160.