ISPsp3
- Family IS5
- Group IS5
Isoform Synonym(s) ISBPH
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AB047327 | ND | Pseudomonas sp. | Pseudomonas sp. KKS102 |
DNA section
IS Length : 1190 bp
Ends
IR Length : 16
IRL : GGATGGTGTTCAAAACTCCGGTCAAGCCCGGATGGCGTGAGCGAGTTACG
IRR : GGATGGTGTTCAAAACACGTTTGGCCCCAGCCGATAGCGGGCGGGTTGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GACGACCGCG | TTAG | ATGTGCAACG | 4 |
DNA sequence
GGATGGTGTTCAAAACTCCGGTCAAGCCCGGATGGCGTGAGCGAGTTACGACACGAACAATGGCGTCATGACCCAACAAGACCTCGGCCTGAACCTGAGC
ACGCGGCGTACTCGCAAAACCGTGTTCCTCGACGAGATGAACCTGGTGGTGCCGTGGACGGAGTTGCTGTCGTTGATTGCGCCGCATGTACCTCGCGCCA
AGACCGGACGCCCGCCGTTTGAGCTGGTGACGATGTTGCGCATTCATTTCTTGCAACAGTGGTTCGGCCTGAGTGACCTGGCCATGGAAGAAGCCCTGTT
CGAGACCACGCTGTACCGCGAGTTCGCGGGACTCTCCAGCGCCGAGCGCATCCCTGACCGGGTCAGCATCCTGCGCTTTCGCCACCTGCTCGAAGAACAC
CAGTTGGCCCCGCAGATGCTGGCCGTGGTCAACGCCACCCTGGCCGACAAAGGCTTGATGCTCAGACAAGGCACGGTGGTGGACGCCACCTTGATTGCTG
CGCCCAGTTCGACCAAGAACCAGGATGGCAAGCGTGATCCCGAGATGCACCAGACCAAGAAGGGCAACCAGTGGCATTTCGGCATGAAAGCGCACATCGG
CGTGGACGCTGACTCGGGACTGGTGCACACCGTGGTCGGTACAGCAGCCAACGTCAACGACGTGACACAGGCCAGTGCGTTGGTCCATGGCGAAGAAACG
GATGTGTTCGCTGACGCCGGCTACCAGGGCGTGACCAAGCGCGAAGAAGTCCAAGGCATCGATGCCAACTGGCATGTGGCCATGCGTCCGGGCAAGCGCC
GCGCGATGGACAAGAACAGTCCCATGGGCGCCGTGCTCGACCAGCTTGAACACGTCAAGGCCCGAATCCGGGCCAAGGTGGAACACCCGTTTCGGGTCAT
CAAACGGCAGTTCGGCCACATGAAAGTGCGCTATCGGGGACTGGCCAAAAACACGGCACAGTTGCACACGCTGTTTGCACTGAGCAATCTTTGGATGGTG
CGACGCCGACTGTTGCAAGGGCTGCAGGCGTGAGTGCGTCCGCAAGCAGCCGAAGGGCCGCCGCCGAACGGAAAATGGCCTGTGAAAACGCAGAAACTGG
GTCGAATTCGCCGATTCCAGGCCGCGCTGTTTCATGTCGTGGCAACCCGCCCGCTATCGGCTGGGGCCAAACGTGTTTTGAACACCATCC
ACGCGGCGTACTCGCAAAACCGTGTTCCTCGACGAGATGAACCTGGTGGTGCCGTGGACGGAGTTGCTGTCGTTGATTGCGCCGCATGTACCTCGCGCCA
AGACCGGACGCCCGCCGTTTGAGCTGGTGACGATGTTGCGCATTCATTTCTTGCAACAGTGGTTCGGCCTGAGTGACCTGGCCATGGAAGAAGCCCTGTT
CGAGACCACGCTGTACCGCGAGTTCGCGGGACTCTCCAGCGCCGAGCGCATCCCTGACCGGGTCAGCATCCTGCGCTTTCGCCACCTGCTCGAAGAACAC
CAGTTGGCCCCGCAGATGCTGGCCGTGGTCAACGCCACCCTGGCCGACAAAGGCTTGATGCTCAGACAAGGCACGGTGGTGGACGCCACCTTGATTGCTG
CGCCCAGTTCGACCAAGAACCAGGATGGCAAGCGTGATCCCGAGATGCACCAGACCAAGAAGGGCAACCAGTGGCATTTCGGCATGAAAGCGCACATCGG
CGTGGACGCTGACTCGGGACTGGTGCACACCGTGGTCGGTACAGCAGCCAACGTCAACGACGTGACACAGGCCAGTGCGTTGGTCCATGGCGAAGAAACG
GATGTGTTCGCTGACGCCGGCTACCAGGGCGTGACCAAGCGCGAAGAAGTCCAAGGCATCGATGCCAACTGGCATGTGGCCATGCGTCCGGGCAAGCGCC
GCGCGATGGACAAGAACAGTCCCATGGGCGCCGTGCTCGACCAGCTTGAACACGTCAAGGCCCGAATCCGGGCCAAGGTGGAACACCCGTTTCGGGTCAT
CAAACGGCAGTTCGGCCACATGAAAGTGCGCTATCGGGGACTGGCCAAAAACACGGCACAGTTGCACACGCTGTTTGCACTGAGCAATCTTTGGATGGTG
CGACGCCGACTGTTGCAAGGGCTGCAGGCGTGAGTGCGTCCGCAAGCAGCCGAAGGGCCGCCGCCGAACGGAAAATGGCCTGTGAAAACGCAGAAACTGG
GTCGAATTCGCCGATTCCAGGCCGCGCTGTTTCATGTCGTGGCAACCCGCCCGCTATCGGCTGGGGCCAAACGTGTTTTGAACACCATCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 966 bp | 321 aa | 68 | 1033 | + | No |
Chemistry : DDE
ORF sequence :
MTQQDLGLNLSTRRTRKTVFLDEMNLVVPWTELLSLIAPHVPRAKTGRPPFELVTMLRIHFLQQWFGLSDLAMEEALFETTLYREFAGLSSAERIPDRVS
ILRFRHLLEEHQLAPQMLAVVNATLADKGLMLRQGTVVDATLIAAPSSTKNQDGKRDPEMHQTKKGNQWHFGMKAHIGVDADSGLVHTVVGTAANVNDVT
QASALVHGEETDVFADAGYQGVTKREEVQGIDANWHVAMRPGKRRAMDKNSPMGAVLDQLEHVKARIRAKVEHPFRVIKRQFGHMKVRYRGLAKNTAQLH
TLFALSNLWMVRRRLLQGLQA
ILRFRHLLEEHQLAPQMLAVVNATLADKGLMLRQGTVVDATLIAAPSSTKNQDGKRDPEMHQTKKGNQWHFGMKAHIGVDADSGLVHTVVGTAANVNDVT
QASALVHGEETDVFADAGYQGVTKREEVQGIDANWHVAMRPGKRRAMDKNSPMGAVLDQLEHVKARIRAKVEHPFRVIKRQFGHMKVRYRGLAKNTAQLH
TLFALSNLWMVRRRLLQGLQA
Blast result :
Comments
This sequence was renammed according to the IS nomenclature.
ISPsp3 is 83% aa similar to IS1405.
ISPsp3 is 83% aa similar to IS1405.
References
1] Ohtsubo,Y., Delawary,M., Kimbara,K., Takagi,M., Ohta,A. and Nagata,Y. (2001) The Journal of biological chemistry. 276 (39), 36146-36154
2] Ohtsubo,Y., Delawary,M., Nagata,Y. and Ohta,A. (2000) Direct submission GenBank
2] Ohtsubo,Y., Delawary,M., Nagata,Y. and Ohta,A. (2000) Direct submission GenBank