ISPpu3
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ233397 | ND | Pseudomonas putida | Pseudomonas putida P1 |
DNA section
IS Length : 1135 bp
Ends
IR Length : 3
IRL : TGTGCATCCCTCCTTTTTCGGCGTCTGTAGCATGGCTCGACCAGCGGCTT
IRR : TGTTTGGTTAGTTCAATAACTTATTCTTGATTGCGGTGAGCTTTGCGCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACAAATATAC | GTCCGCTTGG | 0 |
DNA sequence
TGTGCATCCCTCCTTTTTCGGCGTCTGTAGCATGGCTCGACCAGCGGCTTCTTTTTGTTTAAAACCCACTGATGTTGAGACGCTGCAAAGCTGGTTATGC
ATGGGAACTCTGCCGCAAAACCTTGGGCAACGTGCCCGAGTTCTGTTGCTGCTGGCCAGCGGTCTCACGCCCAAGCAGGTAAGTGATCGGTTGGAAGTTT
CGGCACCTGTCGTATTCAAATGGCGCAAGCGCTATCAGGAAGCTGGCCTCGATGGGCTGAGCGATCTCCCGCGTAGCGGACATCCACGCAAGCTCGACGA
GGCGAAGATTAAGGAAATCCTCACCCTGACGACCCAGCGTGTACCGCGTGAGGCGACACACTGGAGCCTGCGCCTGATGGCCAAATACGTGGGCGTTACC
GTCTGGCAAGTCGCCCAGGTATGGGCCGCTGCCGACCTCAAGCCCCACCGGCTGAAGACCTTCAAGATCAGCAACGACCCACACTTTGCTGGCAAAGTGG
TCGATGTGGTTGGGCTGTACCTGAACCCGCCTGACAATGCGATGGTGCTGTCAGTGGGCGAGAAAACCCAGATCCAGGTGCTGGATCGCACCCAGCCCAT
GCTGCCGCTCAAGCCGGGTCAGATCGAACGCAGGACGCATGACTACAAGCGTCACGGTACGGCCAGTTTGTATGCCGCCTTCGACATCCTGACGGGCCAG
GTCATTGGTCGCATCGCATCACCCAACGACACAGGGCCAAAGAGTTCCTTGAATTCTTGCGACAGATCGACCGCGCGACGCCTGCCGAGCTGGATCTGCA
TTTGATTCTGGACAACAGCTCGACTCACAAGACAGCCGCCGTCAGGGCATTGCTGGAGAGGCATCCCCGCTTCAAGCTGCACTTCACGCCGACCAGTGCC
TCATGATTGAACGCCGTAGAGGGTTGGTTTACTCAATTGGAACGGCGGGCGCTGTATCGCAATGCGTTCAGCAGCGTGGCGGATCTGAAAGCGGCAATTC
GCCGTTTTATCGAAGCCCATAATGAACATTCAGCCAAGCCATTTCGCTGGAACAAAACGGCTGAATCCATCATCAACTCTGTTAGTCGCGCAAAGCTCAC
CGCAATCAAGAATAAGTTATTGAACTAACCAAACA
ATGGGAACTCTGCCGCAAAACCTTGGGCAACGTGCCCGAGTTCTGTTGCTGCTGGCCAGCGGTCTCACGCCCAAGCAGGTAAGTGATCGGTTGGAAGTTT
CGGCACCTGTCGTATTCAAATGGCGCAAGCGCTATCAGGAAGCTGGCCTCGATGGGCTGAGCGATCTCCCGCGTAGCGGACATCCACGCAAGCTCGACGA
GGCGAAGATTAAGGAAATCCTCACCCTGACGACCCAGCGTGTACCGCGTGAGGCGACACACTGGAGCCTGCGCCTGATGGCCAAATACGTGGGCGTTACC
GTCTGGCAAGTCGCCCAGGTATGGGCCGCTGCCGACCTCAAGCCCCACCGGCTGAAGACCTTCAAGATCAGCAACGACCCACACTTTGCTGGCAAAGTGG
TCGATGTGGTTGGGCTGTACCTGAACCCGCCTGACAATGCGATGGTGCTGTCAGTGGGCGAGAAAACCCAGATCCAGGTGCTGGATCGCACCCAGCCCAT
GCTGCCGCTCAAGCCGGGTCAGATCGAACGCAGGACGCATGACTACAAGCGTCACGGTACGGCCAGTTTGTATGCCGCCTTCGACATCCTGACGGGCCAG
GTCATTGGTCGCATCGCATCACCCAACGACACAGGGCCAAAGAGTTCCTTGAATTCTTGCGACAGATCGACCGCGCGACGCCTGCCGAGCTGGATCTGCA
TTTGATTCTGGACAACAGCTCGACTCACAAGACAGCCGCCGTCAGGGCATTGCTGGAGAGGCATCCCCGCTTCAAGCTGCACTTCACGCCGACCAGTGCC
TCATGATTGAACGCCGTAGAGGGTTGGTTTACTCAATTGGAACGGCGGGCGCTGTATCGCAATGCGTTCAGCAGCGTGGCGGATCTGAAAGCGGCAATTC
GCCGTTTTATCGAAGCCCATAATGAACATTCAGCCAAGCCATTTCGCTGGAACAAAACGGCTGAATCCATCATCAACTCTGTTAGTCGCGCAAAGCTCAC
CGCAATCAAGAATAAGTTATTGAACTAACCAAACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1094 bp | 363 aa | 32 | 1125 | + | No |
Chemistry : DDE
ORF sequence :
MARPAASFCLKPTDVETLQSWLCMGTLPQNLGQRARVLLLLASGLTPKQVSDRLEVSAPVVFKWRKRYQEAGLDGLSDLPDARSGHPRKLDEAKIKEILT
LTTQRVPREATHWSLRLMAKYVGVTVWQVAQVWAAADLKPHRLKTFKISNDPHFAGKVVDVVDAGLYLNPPDNAMVLSVGEKTQIQVLDRTQPMLPLKPG
QIERRTHDYKRHGTASLYAAFDILTGQVIGRITQRHRAKEFLEFDALRQIDRATPAELDLHLILDNSSTHKTAAVRALLERHPRFKLHFTPTSASWLNAV
EGWFTQLERRALYRNAFSSVADLKAADAIRRFIEAHNEHSAKPFRWNKTAESIINSVSRAKLTAIKNKLLN
LTTQRVPREATHWSLRLMAKYVGVTVWQVAQVWAAADLKPHRLKTFKISNDPHFAGKVVDVVDAGLYLNPPDNAMVLSVGEKTQIQVLDRTQPMLPLKPG
QIERRTHDYKRHGTASLYAAFDILTGQVIGRITQRHRAKEFLEFDALRQIDRATPAELDLHLILDNSSTHKTAAVRALLERHPRFKLHFTPTSASWLNAV
EGWFTQLERRALYRNAFSSVADLKAADAIRRFIEAHNEHSAKPFRWNKTAESIINSVSRAKLTAIKNKLLN
Blast result :
Comments
ISPpu3 is located next to ISPpu2, between alkS and alkB, genes involved in alkane degradation by Pseudomonas putida P1. The TnpA open-reading-frame has two frameshifts, which implies that IS pep 1 is not produced.
ISPpu3 is 86% aa similar to ISPpu2 and 64% to ISPsy1.
ISPpu3 is 86% aa similar to ISPpu2 and 64% to ISPsy1.
References