ISPg2
- Family ISAs1
- Group
Isoform Synonym(s) PGIS2
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U77885 | Y | Porphyromonas gingivalis | Porphyromonas gingivalis A7436 |
DNA section
IS Length : 1207 bp
Ends
IR Length : 18/19
IRL : CAGGGCTGACGCATTAAAAACAGCTTTCTAAGTACTTTTATAGTCGTTAA
IRR : CAGGGGTGACGCATTAAAATTGTATTGTGATTAGTTGTTGTGGCTTCATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGAGAGAAAG | GGGGACATTG | TCTCTCTTTC | 10 |
DNA sequence
CAGGGCTGACGCATTAAAAACAGCTTTCTAAGTACTTTTATAGTCGTTAAAGCCCCGTTAGTTATGTATCATTCTCTATTTGAAAGCCTCTGCATGGTTC
CGGACCCACGGATAGAGCGTAAAAAAATTTATCCTCTTGATTTTTTACTGCTGATCGTTTTCCTCTCAACACTCTCAGGCAATACCTCTTGGTATGAGAT
TGAAGATTATGCCGAGGAATACGAAGAAGAATTGAAAAGTCTATACGAAATGCTTACCGGCCATCAGCTTATGCATACCATGCCTTCTCATGATACTCTC
AACAGGAGCATCAGTCTGTTGGATGTAGAAGCTTTTGAGGGGGCTTATAAGCGATGGATTGAAGGCTTTATCTCGGCCACTTCGGGTAAACATATTTGCA
TTGATGGCAAGACGATGCGGGGAGTGAAGAAACTCTCTTTCGATACACAATCCCATGTCGTCTCTGCCTTTTCACCACAAGATATGTGCAGTCTTGCCCA
ACTCTACATCGACCGAAAAACAAACGAAATACCGGCTATACATCAACTTCTTGATTTGCTTGACTTGAACGGGGCTGTTGTCTCCATTGATGCCATAGGT
ACACAAACTGCCATTGCCGAACAAATTATCGATAAGGGCGGAAACTATGTATTGTGTGTTAAAGCGAATCAAAGTTTGAGTCTGCAAGAGATTGAAGCCT
ATTTCTGCCCTCTTTTTCAGAAACATATCCTCCTTGACGAACAGACGGAACTATCTCACGGACGCATAGAAACACGTCGCTATGAAAGTATTCTCAATCC
CTTGGAGATAGAAGCCAACGAGGTATTAACTCGCTGGAAAGGCTTGAGGTCGATACACAAAGTTGTACGCAAACGAAGGGATAAAAAGAGTGACAAAACG
AGTGAAGAAGTGGCCTACTACATTTCGTCATTAACAGATGTTTCTTCATTGAAACAAGCTATTCGTGGGCATTGGGCGATAGAGAATAAGTTACACCACT
GTTTGGATGTCTATTTCGGACACGATGCCTCGCACAAGAGAACGAGGAATGTGGCGCAGATTATGGATATCATTCAAAAGATTAATTTACTCATTATAGA
GAGACTAAAAACGAATATGAAGTCCTCAATCCCTCGCATCCAAAAGAAACTGGCTCGAATGAAGCCACAACAACTAATCACAATACAATTTTAATGCGTC
ACCCCTG
CGGACCCACGGATAGAGCGTAAAAAAATTTATCCTCTTGATTTTTTACTGCTGATCGTTTTCCTCTCAACACTCTCAGGCAATACCTCTTGGTATGAGAT
TGAAGATTATGCCGAGGAATACGAAGAAGAATTGAAAAGTCTATACGAAATGCTTACCGGCCATCAGCTTATGCATACCATGCCTTCTCATGATACTCTC
AACAGGAGCATCAGTCTGTTGGATGTAGAAGCTTTTGAGGGGGCTTATAAGCGATGGATTGAAGGCTTTATCTCGGCCACTTCGGGTAAACATATTTGCA
TTGATGGCAAGACGATGCGGGGAGTGAAGAAACTCTCTTTCGATACACAATCCCATGTCGTCTCTGCCTTTTCACCACAAGATATGTGCAGTCTTGCCCA
ACTCTACATCGACCGAAAAACAAACGAAATACCGGCTATACATCAACTTCTTGATTTGCTTGACTTGAACGGGGCTGTTGTCTCCATTGATGCCATAGGT
ACACAAACTGCCATTGCCGAACAAATTATCGATAAGGGCGGAAACTATGTATTGTGTGTTAAAGCGAATCAAAGTTTGAGTCTGCAAGAGATTGAAGCCT
ATTTCTGCCCTCTTTTTCAGAAACATATCCTCCTTGACGAACAGACGGAACTATCTCACGGACGCATAGAAACACGTCGCTATGAAAGTATTCTCAATCC
CTTGGAGATAGAAGCCAACGAGGTATTAACTCGCTGGAAAGGCTTGAGGTCGATACACAAAGTTGTACGCAAACGAAGGGATAAAAAGAGTGACAAAACG
AGTGAAGAAGTGGCCTACTACATTTCGTCATTAACAGATGTTTCTTCATTGAAACAAGCTATTCGTGGGCATTGGGCGATAGAGAATAAGTTACACCACT
GTTTGGATGTCTATTTCGGACACGATGCCTCGCACAAGAGAACGAGGAATGTGGCGCAGATTATGGATATCATTCAAAAGATTAATTTACTCATTATAGA
GAGACTAAAAACGAATATGAAGTCCTCAATCCCTCGCATCCAAAAGAAACTGGCTCGAATGAAGCCACAACAACTAATCACAATACAATTTTAATGCGTC
ACCCCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1131 bp | 376 aa | 64 | 1194 | + | No |
Chemistry : Unknow
ORF sequence :
MYHSLFESLCMVPDPRIERKKIYPLDFLLLIVFLSTLSGNTSWYEIEDYAEEYEEELKSLYEMLTGHQLMHTMPSHDTLNRSISLLDVEAFEGAYKRWIE
GFISATSGKHICIDGKTMRGVKKLSFDTQSHVVSAFSPQDMCSLAQLYIDRKTNEIPAIHQLLDLLDLNGAVVSIDAIGTQTAIAEQIIDKGGNYVLCVK
ANQSLSLQEIEAYFCPLFQKHILLDEQTELSHGRIETRRYESILNPLEIEANEVLTRWKGLRSIHKVVRKRRDKKSDKTSEEVAYYISSLTDVSSLKQAI
RGHWAIENKLHHCLDVYFGHDASHKRTRNVAQIMDIIQKINLLIIERLKTNMKSSIPRIQKKLARMKPQQLITIQF
GFISATSGKHICIDGKTMRGVKKLSFDTQSHVVSAFSPQDMCSLAQLYIDRKTNEIPAIHQLLDLLDLNGAVVSIDAIGTQTAIAEQIIDKGGNYVLCVK
ANQSLSLQEIEAYFCPLFQKHILLDEQTELSHGRIETRRYESILNPLEIEANEVLTRWKGLRSIHKVVRKRRDKKSDKTSEEVAYYISSLTDVSSLKQAI
RGHWAIENKLHHCLDVYFGHDASHKRTRNVAQIMDIIQKINLLIIERLKTNMKSSIPRIQKKLARMKPQQLITIQF
Blast result :
Comments
ISPg2 from Porphyromonas gingivalis (an obligately oral anaerobe Gram-negative cocobacillus, and an important pathogen in human periodontal disease) was found integrated into the transposon vector Tn4351, 110 bp upstream from the ermF gene. At least four ISPg2 copies were found in the wild-type strain Porphyromonas gingivalis A7436 (Wang et al. 1997).
References
1] Genco, C.A., Wang, C.Y., and Bond, V.C. (1996) Poster at XIIth Meeting on Bacterial gene expression and transfer, Siena, September 1996.
2] Wang, C.-Y., Bond, V.C., and Genco, C.A. (1997) J. Bacteriol. 179, 3808-3812.
2] Wang, C.-Y., Bond, V.C., and Genco, C.A. (1997) J. Bacteriol. 179, 3808-3812.