ISMav2
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF286339 | ND | Mycobacterium avium | Mycobacterium avium subsp. Paratuberculosis |
DNA section
IS Length : 1361 bp
Ends
IR Length : 4/5
IRL : TGTATGTATGACGTCGGCTGATGTGTGACGTTTTGTCTTCGGTTGATGTG
IRR : TGTTCAGGGTCGGTTGCTCGGTGACATTTCTATGTCGGTTGATCGGTGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGTCCTAGTG | GGGGCACCAGA | GAGCAGCCCC | 11 |
DNA sequence
TGTATGTATGACGTCGGCTGATGTGTGACGTTTTGTCTTCGGTTGATGTGTGACGGTGTTTCGGTTGATGCGTGACAGTTGTTTCGGCTGATCGGTGACA
CTCCCTAGATGAGGGAGTTAAGTGTGGCTGAGCAGCGGTATCAGGCCGTGATGGCGGTAATCAGTGACGGGTTGTCGGTGTCGCAGGCCGCAGAGAAGTT
CGGGGTGGCGCGTCAGACGCTGCACCGATGGCTGGCCCGGTATGAAGCCGCGGGCCTGAAGGGGCTGGTGGATCGGTCGCATCGGCCGGTGAGTTGTCCG
CATCAGATGCTGGCGGTAGTGGAGGCGGCGGTGTTGGAGTTGCGCCGCTCGCGGCCCTATTGGGGGCCGCGGCGGTTGGTGTTCGAGTTGGCCAAGCGAG
GTGTCCATCCGGTGCCGTCGGAGTCGCCGGTGTATCGAGCGCTGGTGCGGGCCGGTCTGATCGACCCCGCGATGCGAGATCGACGGTCGCGCAAATGGAA
GCGCTGGGAGCGCGGGGCGCCGATGGAGTTGTGGCAGCTCGACATCGTCGGCGGGTTCCCGCTGGCCGATGGGACCAGCGCCAAAGCCCTGACCGGCATC
GATGATCATTCCCGGATGTGTGTGTGCGCCAAGCTGATGGCCCGTGAGCGCACCCGCGCGGTCTGCGACGGATTACGGGCGGCGCTGGCCGCTTACGGGG
TGCCTGAGCAGATCTTGACCGATAACGGCAAGGTGTTCACCGAGCGGTTTTGTCATCCACCGGTCGAGGTGCTCTTTGATGCGATCTGCCGCGAGCACGG
CATCGAACATCTGTTGACCCAGCCGCGCAGCCCGACCACGACCGGCAAAATCGAGCAGTTTCACCGCAGTCTGCGCGCTGAGTTCCTTAGCGGCCGTGAG
CCTTTCACCAACCTCAAGGTCGCTCAGCAGGCGCTCGATGAGTGGGTCGAGGACTACAACACCACCCGGCCGCACCAAGCCCTAAAGATGATCACACCGG
CTCAACGGTTTCACGCCGGTGCGCCGGCATCACCACCGTCGAACTCGTGTGCCCGACACGTCGACCGCAGTGGTGATGACTGGGTGAGTCGGCGGGTGTG
CTCCAACGGCATCGTGTGCGTGTCCTGGCAGCAGGTCTGTATCGGGCGCCACTATGCCGGCGCCCGCTGCGATGTCCACGTCGATGGGGACCTGCTGAGG
TTTTGGGTCGGCGACAATCTGGTCAAAACCGCCGCGCGCACCAGCCGCGGCGAGGTACGAAACAAACAGGCCCTGCGCACCAACGCACCGGCCTAAAACA
CAACCCAGAGTGTCACCGATCAACCGACATAGAAATGTCACCGAGCAACCGACCCTGAACA
CTCCCTAGATGAGGGAGTTAAGTGTGGCTGAGCAGCGGTATCAGGCCGTGATGGCGGTAATCAGTGACGGGTTGTCGGTGTCGCAGGCCGCAGAGAAGTT
CGGGGTGGCGCGTCAGACGCTGCACCGATGGCTGGCCCGGTATGAAGCCGCGGGCCTGAAGGGGCTGGTGGATCGGTCGCATCGGCCGGTGAGTTGTCCG
CATCAGATGCTGGCGGTAGTGGAGGCGGCGGTGTTGGAGTTGCGCCGCTCGCGGCCCTATTGGGGGCCGCGGCGGTTGGTGTTCGAGTTGGCCAAGCGAG
GTGTCCATCCGGTGCCGTCGGAGTCGCCGGTGTATCGAGCGCTGGTGCGGGCCGGTCTGATCGACCCCGCGATGCGAGATCGACGGTCGCGCAAATGGAA
GCGCTGGGAGCGCGGGGCGCCGATGGAGTTGTGGCAGCTCGACATCGTCGGCGGGTTCCCGCTGGCCGATGGGACCAGCGCCAAAGCCCTGACCGGCATC
GATGATCATTCCCGGATGTGTGTGTGCGCCAAGCTGATGGCCCGTGAGCGCACCCGCGCGGTCTGCGACGGATTACGGGCGGCGCTGGCCGCTTACGGGG
TGCCTGAGCAGATCTTGACCGATAACGGCAAGGTGTTCACCGAGCGGTTTTGTCATCCACCGGTCGAGGTGCTCTTTGATGCGATCTGCCGCGAGCACGG
CATCGAACATCTGTTGACCCAGCCGCGCAGCCCGACCACGACCGGCAAAATCGAGCAGTTTCACCGCAGTCTGCGCGCTGAGTTCCTTAGCGGCCGTGAG
CCTTTCACCAACCTCAAGGTCGCTCAGCAGGCGCTCGATGAGTGGGTCGAGGACTACAACACCACCCGGCCGCACCAAGCCCTAAAGATGATCACACCGG
CTCAACGGTTTCACGCCGGTGCGCCGGCATCACCACCGTCGAACTCGTGTGCCCGACACGTCGACCGCAGTGGTGATGACTGGGTGAGTCGGCGGGTGTG
CTCCAACGGCATCGTGTGCGTGTCCTGGCAGCAGGTCTGTATCGGGCGCCACTATGCCGGCGCCCGCTGCGATGTCCACGTCGATGGGGACCTGCTGAGG
TTTTGGGTCGGCGACAATCTGGTCAAAACCGCCGCGCGCACCAGCCGCGGCGAGGTACGAAACAAACAGGCCCTGCGCACCAACGCACCGGCCTAAAACA
CAACCCAGAGTGTCACCGATCAACCGACATAGAAATGTCACCGAGCAACCGACCCTGAACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1188 bp | 395 aa | 109 | 1296 | + | No |
Chemistry : DDE
ORF sequence :
MRELSVAEQRYQAVMAVISDGLSVSQAAEKFGVARQTLHRWLARYEAAGLKGLVDRSHRPVSCPHQMLAVVEAAVLELRRSRPYWGPRRLVFELAKRGVH
PVPSESPVYRALVRAGLIDPAMRDRRSRKWKRWERGAPMELWQLDIVGGFPLADGTSAKALTGIDDHSRMCVCAKLMARERTRAVCDGLRAALAAYGVPE
QILTDNGKVFTERFCHPPVEVLFDAICREHGIEHLLTQPRSPTTTGKIEQFHRSLRAEFLSGREPFTNLKVAQQALDEWVEDYNTTRPHQALKMITPAQR
FHAGAPASPPSNSCARHVDRSGDDWVSRRVCSNGIVCVSWQQVCIGRHYAGARCDVHVDGDLLRFWVGDNLVKTAARTSRGEVRNKQALRTNAPA
PVPSESPVYRALVRAGLIDPAMRDRRSRKWKRWERGAPMELWQLDIVGGFPLADGTSAKALTGIDDHSRMCVCAKLMARERTRAVCDGLRAALAAYGVPE
QILTDNGKVFTERFCHPPVEVLFDAICREHGIEHLLTQPRSPTTTGKIEQFHRSLRAEFLSGREPFTNLKVAQQALDEWVEDYNTTRPHQALKMITPAQR
FHAGAPASPPSNSCARHVDRSGDDWVSRRVCSNGIVCVSWQQVCIGRHYAGARCDVHVDGDLLRFWVGDNLVKTAARTSRGEVRNKQALRTNAPA
Blast result :
Comments
ISMav2 is 65% aa similar to ISSco2. ISMav2 is present in at least 3 copies on the genome.
Inverted repeats: TGA TCG GTG ACA CTC; positions: IRL 88 bp downstream of DRL (position 891 of AF286339); IRR 39 bp upstream of DRR (position 2109 of AF286339)
Homology of the putative transposase (ORF1) to a putative transposase of Streptomyces coelicolor (T35457, 60,3% along 273 aa; Al109949, position 18050 to 19130, 59,4% along 1086 bp)
ISMav2 was found in all 79 tested M. avium ssp. paratuberculosis strains,but in none of 10 M. avium ssp. avium strains and none of 10 tested reference strains of 10 other mycobacteria species.
Inverted repeats: TGA TCG GTG ACA CTC; positions: IRL 88 bp downstream of DRL (position 891 of AF286339); IRR 39 bp upstream of DRR (position 2109 of AF286339)
Homology of the putative transposase (ORF1) to a putative transposase of Streptomyces coelicolor (T35457, 60,3% along 273 aa; Al109949, position 18050 to 19130, 59,4% along 1086 bp)
ISMav2 was found in all 79 tested M. avium ssp. paratuberculosis strains,but in none of 10 M. avium ssp. avium strains and none of 10 tested reference strains of 10 other mycobacteria species.
References
1] Strommenger,B., Stevenson,K. and Gerlach,G.-F.(2001) FEMS Microbiol. Lett. 196 (1), 31-37