ISHs1
- Family ISH6
- Group
Isoform Synonym(s) ISHS1
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| M38315 | ND | Halobacterium salinarum | Halobacterium salinarum S1 |
DNA section
IS Length : 1449 bp
Ends
IR Length : 24/27
IRL : GGTCGTAACGCAAAGCGGTCTAGAGTTCAGTAGCAACTGGCTCCCGTGAG
IRR : GGTCGCGTCGCAAAGCGGTCTAGAGTTTGCCTCTGGTGCTCTCAACTGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACCCAGATGC | AAAGAAAT | TCTACGCCAN | 8 |
DNA sequence
GGTCGTAACGCAAAGCGGTCTAGAGTTCAGTAGCAACTGGCTCCCGTGAGGAACGGGACGCCTCTGGTGTCCACCCAAGAGGTGTCCCATGCACGCCACA
ATCGACGTGCGGTTGACCGTTAGCATCGACGACGACAAAACGATACCGCTGGCCACGCTTGCCGAGTTCATCACTGACCAGAACGTTGAATCAGTTCTTC
TCGAAGGACTCGTCGAGAGCCTCGACGCGGCTCGCGTCGAGGCGCTCTGTGGCGAGAAACACGCTCACGGCAATGGTGATCAGCGATTCCAACGCGCCGG
TACCGACACCCGCACAGCCGTCACAACCGCCGGTGAACACGAATTCGACCTTCACTTACGTCGAAGATACCGCCGCTGACCACGACGAATCCAGCTACTT
CCGCCCCGTCGAAGATGTTCTCAGCTTCGACGGGCAAAACCGCTATCAGCAGGACATTGCGGCCAAGAGCGTCGATCTCGCCACCTCGCTCAGCTACCGT
GATGCTGCTGACCACGGCGACGGCATCCTCTCGAAGATGCCGTCGCCGACCACCATTAACCGCCGCGCCAGAGAATACGGCAGCAAGCTCAAGCAGTTCC
TTCCAGACTGTGTCGCTGACACAGACGCTGACGCCGTTATTCCTGACGGCACGAAGGTTCACAGCCAAGACGACGACCGCACGTACCACTCCGTCCAAGC
CACGCTCGGCGAAGACACCGCTGAGGAATCACGCTCTCTACTGGATCTCTCGGTGAACGCTGACTGGGACCAAACAGCAGCCGATCTCGATGATATCGAC
GCAGTCACTGACGACGCGACGGTCGTCAGTGACGCTGATGACGGCATCGTTACGGCCTTTACCGACGAATACAGCGATCACCAACTCGATCTCGTCCACG
TCGGCCGAACGCTGGACTACAACCTCTGGGACGACGGCGTGTTCTCCTTGGATCGACGGAACGAGATCGTCTCGGAGGTGATCGACGAGGTGTTCCATCT
GAAGAATTCGGTCGCCAAGCACCGTCCAAACGAGGAGTTCGCGGCGATCCGCGAACGGATCGCGCGAACGACCGAGCGTATCGAAAAGACAGCCTGGCAG
TTGGATCAGTACGGGTCAGAGAAGGCTGCGGGGTATCTTCGGCGGTGGCTGCCGTCGATCGTGACGTTTGCCGAGCAGGCTGCCGAGGGGTTCGAGGTGC
CGTGGACCTCGAACCCCGTCGAACGGCTGATGGGGGAAGTCAGCAAGCGCTGTAAGAACCAGTGGATGCGCTGGACAACGGAGGGATTAGAGGCGATACT
CCAGCTTCGGCTGGTGAAGTACGCCGACCCAGAGTACTACCAGTCGTTCCTCGACGAATTGCTCCAGCGATCGACCAAAACAGCAATGAGCTGTGACCTC
TCAGTTGAGAGCACCAGAGGCAAACTCTAGACCGCTTTGCGACGCGACC
ATCGACGTGCGGTTGACCGTTAGCATCGACGACGACAAAACGATACCGCTGGCCACGCTTGCCGAGTTCATCACTGACCAGAACGTTGAATCAGTTCTTC
TCGAAGGACTCGTCGAGAGCCTCGACGCGGCTCGCGTCGAGGCGCTCTGTGGCGAGAAACACGCTCACGGCAATGGTGATCAGCGATTCCAACGCGCCGG
TACCGACACCCGCACAGCCGTCACAACCGCCGGTGAACACGAATTCGACCTTCACTTACGTCGAAGATACCGCCGCTGACCACGACGAATCCAGCTACTT
CCGCCCCGTCGAAGATGTTCTCAGCTTCGACGGGCAAAACCGCTATCAGCAGGACATTGCGGCCAAGAGCGTCGATCTCGCCACCTCGCTCAGCTACCGT
GATGCTGCTGACCACGGCGACGGCATCCTCTCGAAGATGCCGTCGCCGACCACCATTAACCGCCGCGCCAGAGAATACGGCAGCAAGCTCAAGCAGTTCC
TTCCAGACTGTGTCGCTGACACAGACGCTGACGCCGTTATTCCTGACGGCACGAAGGTTCACAGCCAAGACGACGACCGCACGTACCACTCCGTCCAAGC
CACGCTCGGCGAAGACACCGCTGAGGAATCACGCTCTCTACTGGATCTCTCGGTGAACGCTGACTGGGACCAAACAGCAGCCGATCTCGATGATATCGAC
GCAGTCACTGACGACGCGACGGTCGTCAGTGACGCTGATGACGGCATCGTTACGGCCTTTACCGACGAATACAGCGATCACCAACTCGATCTCGTCCACG
TCGGCCGAACGCTGGACTACAACCTCTGGGACGACGGCGTGTTCTCCTTGGATCGACGGAACGAGATCGTCTCGGAGGTGATCGACGAGGTGTTCCATCT
GAAGAATTCGGTCGCCAAGCACCGTCCAAACGAGGAGTTCGCGGCGATCCGCGAACGGATCGCGCGAACGACCGAGCGTATCGAAAAGACAGCCTGGCAG
TTGGATCAGTACGGGTCAGAGAAGGCTGCGGGGTATCTTCGGCGGTGGCTGCCGTCGATCGTGACGTTTGCCGAGCAGGCTGCCGAGGGGTTCGAGGTGC
CGTGGACCTCGAACCCCGTCGAACGGCTGATGGGGGAAGTCAGCAAGCGCTGTAAGAACCAGTGGATGCGCTGGACAACGGAGGGATTAGAGGCGATACT
CCAGCTTCGGCTGGTGAAGTACGCCGACCCAGAGTACTACCAGTCGTTCCTCGACGAATTGCTCCAGCGATCGACCAAAACAGCAATGAGCTGTGACCTC
TCAGTTGAGAGCACCAGAGGCAAACTCTAGACCGCTTTGCGACGCGACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1182 bp | 393 aa | 249 | 1430 | + | No |
Chemistry : DDE
ORF sequence :
MARNTLTAMVISDSNAPVPTPAQPSQPPVNTNSTFTYVEDTAADHDESSYFRPVEDVLSFDGQNRYQQDIAAKSVDLATSLSYRDAADHGDGILSKMPSP
TTINRRAREYGSKLKQFLPDCVADTDADAVIPDGTKVHSQDDDRTYHSVQATLGEDTAEESRSLLDLSVNADWDQTAADLDDIDAVTDDATVVSDADDGI
VTAFTDEYSDHQLDLVHVGRTLDYNLWDDGVFSLDRRNEIVSEVIDEVFHLKNSVAKHRPNEEFAAIRERIARTTERIEKTAWQLDQYGSEKAAGYLRRW
LPSIVTFAEQAAEGFEVPWTSNPVERLMGEVSKRCKNQWMRWTTEGLEAILQLRLVKYADPEYYQSFLDELLQRSTKTAMSCDLSVESTRGKL
TTINRRAREYGSKLKQFLPDCVADTDADAVIPDGTKVHSQDDDRTYHSVQATLGEDTAEESRSLLDLSVNADWDQTAADLDDIDAVTDDATVVSDADDGI
VTAFTDEYSDHQLDLVHVGRTLDYNLWDDGVFSLDRRNEIVSEVIDEVFHLKNSVAKHRPNEEFAAIRERIARTTERIEKTAWQLDQYGSEKAAGYLRRW
LPSIVTFAEQAAEGFEVPWTSNPVERLMGEVSKRCKNQWMRWTTEGLEAILQLRLVKYADPEYYQSFLDELLQRSTKTAMSCDLSVESTRGKL
Blast result :
Comments
ISHs1 has been isolated through its inactivation of the bacteriorhodopsin (bop) gene. ISHs1 might be distantly related to the IS256 family.
References
1] Ovchinnikov, Y.A., Zozulya, S.A., Zaitseva, E.M., Gur'ev, S.O., Sverdlov, E.D., Krupenko, M.A., and Aleksandrov, A.A. (1984) Bioorg. Khim. 10, 560-563.
2] Zvyaga, T.A., Zozulya, S.A., and Gur'ev, S.O. (1987) Bioorg. Khim. 13, 1351-1357.
3] DasSarma, S. (1989) Can. J. Microbiol. 35, 65-72.
2] Zvyaga, T.A., Zozulya, S.A., and Gur'ev, S.O. (1987) Bioorg. Khim. 13, 1351-1357.
3] DasSarma, S. (1989) Can. J. Microbiol. 35, 65-72.