ISH8E
- Family IS4
- Group ISH8
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF016485 | ND | Halobacterium sp. | Halobacterium sp. NRC-1 plasmid pNRC100 Halobacterium sp. NRC-1 |
DNA section
IS Length : 1403 bp
Ends
IR Length : 15
IRL : CATCCGTCTTTAGTTAGTCGAGGAATCGCGTGACAGCAGCGGCTTATCGA
IRR : CATCCGTCTTTAGTTAGCCCTCACACCTCGGTTGTGTAGCGGTAGCGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
CATCCGTCTTTAGTTAGTCGAGGAATCGCGTGACAGCAGCGGCTTATCGAGGCTATTTTTCGAGAGGAATGAGGAGGCGGCAAGTGTGCACCAAGACGCC
TCCCCATCGAGAATTATGCGTCGGCTCACTACACTGTTTCCCTCTGAGTTCCTCGAAGAGCACGCCGAGGAACTCGGCGTGGTCGAACGCAACCGTACGC
TCCAGATACCCGTCCTCGTATGGGCGCTCGTGTTCGGCTTCGCCGCAGGCGAGAGCCGAACACTCGCTGGGTTCAGGCGGAGCTACAACGCCACAGCTGA
CGAGCCGATCTCTCCTGGCGGGTTCTATCAGCGATTGACGCCGTCTCTCGCTACGTATCTCCGCGACCTCGTCGAGCGTGGTCTCGACGAGGTCGCTGTT
CCAAACGCTGTGGACGCTGATATCGACCGATTCAGAGACGTGATGATCGCTGATGGCACCGTGTTGCGGTTGCACGAGTTCCTCTCTGAGGTGTACGAAG
GCCGCCACGAGGAGCAGGCTGGAGCACGGCTCCACCTGCTCCACAATGCCACCGAGCAGATGATTGAACGGATCGACGTGACCGACGAGACAGCCCACGA
CAGCACGTTGTTCAAGACGGGTTCGTGGCTGCACGGACGGCTGATTTTGCTTGATCTGGCGTATTTCAAGTACCGCCGCTTCGCGTTGATCGACGAGAAC
GACGGCTTCTTCGTGAGTCGGCTGAAGCAGAACGCGAATCCGGTGATAACGGCGGAATTACGGGAATGGCGCGGGCGCGCCATTCCCTTGGAAGGCAAGC
AGATCCAGGATGTAGTCGACGACCTCTCGCGGCAGTACATCGACGTCGAGGTCGAAGCAGAGTTCAAGCGCGGGCAGTACGAGGGAACGCGGTCATTGGA
CACGAAGCGGTTCCGCGTCGTCGGCGTCCGCAACGAGGACGCCGACGACTACCATCTCTACATAACGAATCTGCCGAGAGACGAGTTCCTGCCGTCAGAC
CTAGCGACGCTGTACCGGTGTCGATGGGAGGTAGAAACGTTGTTCCGTGAGCTGAAGACGCAGTACGAACTGGACGAATTCGACACGAGAAACCCGGCTG
TCGTGGAAATCTTGCTGTACGCGGCGTTGCTGTCACTGCTGGTGAGTCGTGAACTGTTGGATCTGGTCACCGAGCAGGCAGACGATGAGATCGTGTTTCC
GCCGGAACGCTGGGCGGCGACCTTCCGGTCGCACGCCCAGCTCATCCTCCACGAACTCGGAGAGTACCTCGGCTACTCGCCACCGCCGTTGCTGGAACGG
CTGATCGACGACGCACAGAAGATCCACCAGCAACGACCGATCTTACAAGAGACGCTCGCTACCGCTACACAACCGAGGTGTGAGGGCTAACTAAAGACGG
ATG
TCCCCATCGAGAATTATGCGTCGGCTCACTACACTGTTTCCCTCTGAGTTCCTCGAAGAGCACGCCGAGGAACTCGGCGTGGTCGAACGCAACCGTACGC
TCCAGATACCCGTCCTCGTATGGGCGCTCGTGTTCGGCTTCGCCGCAGGCGAGAGCCGAACACTCGCTGGGTTCAGGCGGAGCTACAACGCCACAGCTGA
CGAGCCGATCTCTCCTGGCGGGTTCTATCAGCGATTGACGCCGTCTCTCGCTACGTATCTCCGCGACCTCGTCGAGCGTGGTCTCGACGAGGTCGCTGTT
CCAAACGCTGTGGACGCTGATATCGACCGATTCAGAGACGTGATGATCGCTGATGGCACCGTGTTGCGGTTGCACGAGTTCCTCTCTGAGGTGTACGAAG
GCCGCCACGAGGAGCAGGCTGGAGCACGGCTCCACCTGCTCCACAATGCCACCGAGCAGATGATTGAACGGATCGACGTGACCGACGAGACAGCCCACGA
CAGCACGTTGTTCAAGACGGGTTCGTGGCTGCACGGACGGCTGATTTTGCTTGATCTGGCGTATTTCAAGTACCGCCGCTTCGCGTTGATCGACGAGAAC
GACGGCTTCTTCGTGAGTCGGCTGAAGCAGAACGCGAATCCGGTGATAACGGCGGAATTACGGGAATGGCGCGGGCGCGCCATTCCCTTGGAAGGCAAGC
AGATCCAGGATGTAGTCGACGACCTCTCGCGGCAGTACATCGACGTCGAGGTCGAAGCAGAGTTCAAGCGCGGGCAGTACGAGGGAACGCGGTCATTGGA
CACGAAGCGGTTCCGCGTCGTCGGCGTCCGCAACGAGGACGCCGACGACTACCATCTCTACATAACGAATCTGCCGAGAGACGAGTTCCTGCCGTCAGAC
CTAGCGACGCTGTACCGGTGTCGATGGGAGGTAGAAACGTTGTTCCGTGAGCTGAAGACGCAGTACGAACTGGACGAATTCGACACGAGAAACCCGGCTG
TCGTGGAAATCTTGCTGTACGCGGCGTTGCTGTCACTGCTGGTGAGTCGTGAACTGTTGGATCTGGTCACCGAGCAGGCAGACGATGAGATCGTGTTTCC
GCCGGAACGCTGGGCGGCGACCTTCCGGTCGCACGCCCAGCTCATCCTCCACGAACTCGGAGAGTACCTCGGCTACTCGCCACCGCCGTTGCTGGAACGG
CTGATCGACGACGCACAGAAGATCCACCAGCAACGACCGATCTTACAAGAGACGCTCGCTACCGCTACACAACCGAGGTGTGAGGGCTAACTAAAGACGG
ATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1275 bp | 424 aa | 116 | 1390 | + | No |
Chemistry : DDE
ORF sequence :
MRRLTTLFPSEFLEEHAEELGVVERNRTLQIPVLVWALVFGFAAGESRTLAGFRRSYNATADEPISPGGFYQRLTPSLATYLRDLVERGLDEVAVPNAVD
ADIDRFRDVMIADGTVLRLHEFLSEVYEGRHEEQAGARLHLLHNATEQMIERIDVTDETAHDSTLFKTGSWLHGRLILLDLAYFKYRRFALIDENDGFFV
SRLKQNANPVITAELREWRGRAIPLEGKQIQDVVDDLSRQYIDVEVEAEFKRGQYEGTRSLDTKRFRVVGVRNEDADDYHLYITNLPRDEFLPSDLATLY
RCRWEVETLFRELKTQYELDEFDTRNPAVVEILLYAALLSLLVSRELLDLVTEQADDEIVFPPERWAATFRSHAQLILHELGEYLGYSPPPLLERLIDDA
QKIHQQRPILQETLATATQPRCEG
ADIDRFRDVMIADGTVLRLHEFLSEVYEGRHEEQAGARLHLLHNATEQMIERIDVTDETAHDSTLFKTGSWLHGRLILLDLAYFKYRRFALIDENDGFFV
SRLKQNANPVITAELREWRGRAIPLEGKQIQDVVDDLSRQYIDVEVEAEFKRGQYEGTRSLDTKRFRVVGVRNEDADDYHLYITNLPRDEFLPSDLATLY
RCRWEVETLFRELKTQYELDEFDTRNPAVVEILLYAALLSLLVSRELLDLVTEQADDEIVFPPERWAATFRSHAQLILHELGEYLGYSPPPLLERLIDDA
QKIHQQRPILQETLATATQPRCEG
Blast result :
Comments
One copie is present in pNRC100 of Halobacterium sp. NRC-1 at coordinates 79702-81104. 91% aa homologous with ISH8B , 92% aa with ISH8C and 90% aa with ISH8D.
References
1] Wailap, V. Ng., Stacy A. Ciufo, Smith, T.M., Bumgarner, R.E., Baskin, D., Faust, J., Hall, B., Loretz, C., Seto, J., Slagel, J., Hood, L., and DasSarma, S. (1998) Genome Res. 8, 1131-1141