ISH8D
- Family IS4
- Group ISH8
Isoform ISH26Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF016485 | ND | Halobacterium sp. | Halobacterium sp. NRC-1 plasmid pNRC100 Halobacterium sp. NRC-1 |
DNA section
IS Length : 1402 bp
Ends
IR Length : 18
IRL : CATCCGTCTTTAGTTAAGCGAGAAACCGCGTGATAGCGGCGGCTTATCGA
IRR : CATTCGTCTTCAGTTAAGACTCACACCTCGGTTGTGTAGCGGTAGCGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
CATCCGTCTTTAGTTAAGCGAGAAACCGCGTGATAGCGGCGGCTTATCGAGGCACCTTTCGGAAGGAATGAGGAAGTCCAGTCTGTGCACGACAAGGACT
CCTCATCTCGGATTATGCGTCGGCTCACTACACTGTTTCCCTCTGAGTTCCTTGAAGAGCACGCCGAGGAACTCGGCGTGGTCGAACGTGACCGCAAGCT
CCAGATCCCTGCCTTCGTTTGGGCATTCGTGTTCGGCTTCGCCGCAGGCGAAAGCCGAACACTCGCCGGGTTTAGACGCTGTTACAACTCGACTGCCGAT
GAGACGATCTCTCCGGGTGGATTCTATCAGCGGTTGACACCGACGCTTGCGGAGTATCTCCGCGACCTCGTTGAGCATGGTCTCGACGAGGTCGCTGTTC
CTAACGCTGTTGACGCTGATATCGACCGATTTAGAGACGTGATGATCGCTGATGGAACGGTGTTGCGGTTACACGAATTTCTCTCAGATCAGTTCGAAGC
CCGCCACGAGGAGCAGGCTGGAGCGAAGCTCCACCTGCTCCACAATGCCACAGAGCAGACGATTGAACGGCTCGATACTGCTAACGAGAAAACGCACGAC
AGCACCTTGTTCAAAACAGGGCCGTGGCTTGAGAATCGCCTCGTGTTGTTCGATCTCGCCTACTTCAAGTACCGCCGGTTTGCGCTGATCGACGAAAACG
ACGGCTACTTCGTGAGTCGGCTGAAGCAGAACGCGAATCCGCTGATTACGAGGGAGTTACGGGAATGGCGCGGTCGCGCCATTCCCTTAGAGGGCAAGCA
GCTCCGAGCTGTTCTCGACGATCTCGATCGGAAGTACATCGATGTAGAAGTCGAAGTCGAATTCAAACGAGGGCCGTACAATGGGACGCGGTCACTAGAT
ACGAAGCGATTTCGCGTCGTCGGCGTCCGCGACGAAGACGCCGACGACTACCATCTGTACATGACGAATTTGGTGAGAAAAGAGTTCTTCCCGGCTGATT
TAGCACAGATCTACCGATGTCGGTGGGAAGTTGAGTTGTTGTTCCGTGAGCTGAAGACGCAGTACAACTTGGATGAGTTCAACACGAGCGACGAACACGT
GGTGAAGATCTTACTATACGCAGCACTGCTGTCGCTGCTTGTAAGCCGCGATTTATTGGATCTGGTCACTGAGCAGGCGGATGATGAGCTCGTGTTTCCG
ACAGAACGCTGGGCGGCGACCTTCCGGTCGCACGCCCAGCTTTTCCTCCACAAACTGGGTGAGTATCTCGGCTACTCACCACCGCCGCTGCTTGAACGAC
TGACTGAAGACGCTCAAAAGATCCACAAGCAGCGACCGATCTTACAAGAGACGCTCGCTACCGCTACACAACCGAGGTGTGAGTCTTAACTGAAGACGAA
TG
CCTCATCTCGGATTATGCGTCGGCTCACTACACTGTTTCCCTCTGAGTTCCTTGAAGAGCACGCCGAGGAACTCGGCGTGGTCGAACGTGACCGCAAGCT
CCAGATCCCTGCCTTCGTTTGGGCATTCGTGTTCGGCTTCGCCGCAGGCGAAAGCCGAACACTCGCCGGGTTTAGACGCTGTTACAACTCGACTGCCGAT
GAGACGATCTCTCCGGGTGGATTCTATCAGCGGTTGACACCGACGCTTGCGGAGTATCTCCGCGACCTCGTTGAGCATGGTCTCGACGAGGTCGCTGTTC
CTAACGCTGTTGACGCTGATATCGACCGATTTAGAGACGTGATGATCGCTGATGGAACGGTGTTGCGGTTACACGAATTTCTCTCAGATCAGTTCGAAGC
CCGCCACGAGGAGCAGGCTGGAGCGAAGCTCCACCTGCTCCACAATGCCACAGAGCAGACGATTGAACGGCTCGATACTGCTAACGAGAAAACGCACGAC
AGCACCTTGTTCAAAACAGGGCCGTGGCTTGAGAATCGCCTCGTGTTGTTCGATCTCGCCTACTTCAAGTACCGCCGGTTTGCGCTGATCGACGAAAACG
ACGGCTACTTCGTGAGTCGGCTGAAGCAGAACGCGAATCCGCTGATTACGAGGGAGTTACGGGAATGGCGCGGTCGCGCCATTCCCTTAGAGGGCAAGCA
GCTCCGAGCTGTTCTCGACGATCTCGATCGGAAGTACATCGATGTAGAAGTCGAAGTCGAATTCAAACGAGGGCCGTACAATGGGACGCGGTCACTAGAT
ACGAAGCGATTTCGCGTCGTCGGCGTCCGCGACGAAGACGCCGACGACTACCATCTGTACATGACGAATTTGGTGAGAAAAGAGTTCTTCCCGGCTGATT
TAGCACAGATCTACCGATGTCGGTGGGAAGTTGAGTTGTTGTTCCGTGAGCTGAAGACGCAGTACAACTTGGATGAGTTCAACACGAGCGACGAACACGT
GGTGAAGATCTTACTATACGCAGCACTGCTGTCGCTGCTTGTAAGCCGCGATTTATTGGATCTGGTCACTGAGCAGGCGGATGATGAGCTCGTGTTTCCG
ACAGAACGCTGGGCGGCGACCTTCCGGTCGCACGCCCAGCTTTTCCTCCACAAACTGGGTGAGTATCTCGGCTACTCACCACCGCCGCTGCTTGAACGAC
TGACTGAAGACGCTCAAAAGATCCACAAGCAGCGACCGATCTTACAAGAGACGCTCGCTACCGCTACACAACCGAGGTGTGAGTCTTAACTGAAGACGAA
TG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1275 bp | 424 aa | 114 | 1388 | + | No |
Chemistry : DDE
ORF sequence :
MRRLTTLFPSEFLEEHAEELGVVERDRKLQIPAFVWAFVFGFAAGESRTLAGFRRCYNSTADETISPGGFYQRLTPTLAEYLRDLVEHGLDEVAVPNAVD
ADIDRFRDVMIADGTVLRLHEFLSDQFEARHEEQAGAKLHLLHNATEQTIERLDTANEKTHDSTLFKTGPWLENRLVLFDLAYFKYRRFALIDENDGYFV
SRLKQNANPLITRELREWRGRAIPLEGKQLRAVLDDLDRKYIDVEVEVEFKRGPYNGTRSLDTKRFRVVGVRDEDADDYHLYMTNLVRKEFFPADLAQIY
RCRWEVELLFRELKTQYNLDEFNTSDEHVVKILLYAALLSLLVSRDLLDLVTEQADDELVFPTERWAATFRSHAQLFLHKLGEYLGYSPPPLLERLTEDA
QKIHKQRPILQETLATATQPRCES
ADIDRFRDVMIADGTVLRLHEFLSDQFEARHEEQAGAKLHLLHNATEQTIERLDTANEKTHDSTLFKTGPWLENRLVLFDLAYFKYRRFALIDENDGYFV
SRLKQNANPLITRELREWRGRAIPLEGKQLRAVLDDLDRKYIDVEVEVEFKRGPYNGTRSLDTKRFRVVGVRDEDADDYHLYMTNLVRKEFFPADLAQIY
RCRWEVELLFRELKTQYNLDEFNTSDEHVVKILLYAALLSLLVSRDLLDLVTEQADDELVFPTERWAATFRSHAQLFLHKLGEYLGYSPPPLLERLTEDA
QKIHKQRPILQETLATATQPRCES
Blast result :
Comments
One copie is present in pNRC100 of Halobacterium sp. NRC-1 at coordinates 14194-15595. 97% aa homologous with ISH8A , 93% aa with ISH8B and 93% ADN with ISH26 , 91% aa with ISH8C. 99% ADN homologous with ISH8 (7 deletions and 2 differencies).
References
1] Wailap, V. Ng., Stacy A. Ciufo, Smith, T.M., Bumgarner, R.E., Baskin, D., Faust, J., Hall, B., Loretz, C., Seto, J., Slagel, J., Hood, L., and DasSarma, S. (1998) Genome Res. 8, 1131-1141