ISH8C
- Family IS4
- Group ISH8
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF016485 | ND | Halobacterium sp. | Halobacterium sp. NRC-1 plasmid pNRC100 Halobacterium sp. NRC-1 |
DNA section
IS Length : 1403 bp
Ends
IR Length : 20
IRL : CATTCGTCTTTAGCTAAGACGAGAACCACGTGACAGCAGCGGCTTATCGA
IRR : CATTCGTCTTTAGCTAAGACTCACACCTCGGTTGTGTAGCGGTAGCGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
CATTCGTCTTTAGCTAAGACGAGAACCACGTGACAGCAGCGGCTTATCGAGGCTACTTTTCGGAAGGAGTGAGGAGGAGAAGTCTGTGCACGACTCTCGC
TCCTCATCGAGAATCATGCGTCGGCTCACTACACTGTTTCCCTCCAAGTTCCTCGAAGAGCACGCCGAGGAACTCGGCGTGGTCGAGCGTGAGGGGAAGC
TTCAGATTCCTGTCCTCGTGTGGGCGCTCGTGTTCGGCTTCGCCGCAGGCGAGAGCCGAACACTCGCCGGGTTCAGACGCTGCTACAACTCCACAGCTGA
TGAAACGATCTCTCCCGGTGGCTTCTATCACCGGTTGACACCAACGCTGGCGGAGTACCTCCGCGACCTCGTTGAGCACGGTCTCGACGAGGTCGCTGTT
CCCGACACTGTTGACGCTGATATCGACCGATTCAGGGACGTGATGATCGCCGATGGAACGGTATTGCGGTTGCACGAGTTCCTTTCTGATGAGTTCCAAG
CCCGTCACGAGGAGCAGGCTGGAGCGAAGCTCCACCTGCTCCACAACGCCACCGATGAGACGATTGAACGGATCGATGTGACGGACGAGAAAACGCACGA
CAGCACGCTGTTCAAAACGGGCTCGTGGCTACAAGAACGGCTCGTTCTGTTCGATCGAGCGTACTTCAAGTACCGCCGCTTCGCGTTGATCGATGAGAAC
GACGGCTACTTCGTGAGTCGGCTGAAAGAAAACGCGAATCCGCTGATAACGGAGGAATTGCGGGAATGGCGCGGCCGCGCCATTCCCTTGGAGGGCAAGC
AGATCCACGATGTGGTCGATGATATCTCGCGGAAGTACATCGACGTAGAGGTCGAAGCGGAGTTCAAGCGGGGCCAGTACGAGGGAACGCGCTCGCTGGA
TACGAAGCGATTCCGCGTCGTCGGCGTCCGCGATTCGGACGCCGACGACTACCATCTCTACATTACGAATCTGCCGCGAGATGAGTTCTTCCCGGAGGAT
CTAGCAACGTTGTATCGGTGTCGTTGGGAGGTAGAAACGCTGTTTCGTGAACTGAAGACGCAGTACGAGTTGGACGAGTTCAACACGAGTGACCCGGATG
TGGTGAAAATTCTGCTGTATGCGGCGTTGCTGTCACTGCTGGTGAGTCGTGAGCTGTTGGATCTGGTCACCGAACAGGCCGATGACGAAATCGTGTTTCC
ACCGGAACGCTGGGCGGCGACCTTCCGGTCGCACGCCCAGCTCATCCTCCAGGAACTCGGTGAGTATCTCGGTTACTCGCCACCGCCGTTGCTGGAGCGG
CTGATCGAGGACGCTCAGAAGATTCACCAGCAACGACCGATCTTACAGGAGACGCTCGCTACCGCTACACAACCGAGGTGTGAGTCTTAGCTAAAGACGA
ATG
TCCTCATCGAGAATCATGCGTCGGCTCACTACACTGTTTCCCTCCAAGTTCCTCGAAGAGCACGCCGAGGAACTCGGCGTGGTCGAGCGTGAGGGGAAGC
TTCAGATTCCTGTCCTCGTGTGGGCGCTCGTGTTCGGCTTCGCCGCAGGCGAGAGCCGAACACTCGCCGGGTTCAGACGCTGCTACAACTCCACAGCTGA
TGAAACGATCTCTCCCGGTGGCTTCTATCACCGGTTGACACCAACGCTGGCGGAGTACCTCCGCGACCTCGTTGAGCACGGTCTCGACGAGGTCGCTGTT
CCCGACACTGTTGACGCTGATATCGACCGATTCAGGGACGTGATGATCGCCGATGGAACGGTATTGCGGTTGCACGAGTTCCTTTCTGATGAGTTCCAAG
CCCGTCACGAGGAGCAGGCTGGAGCGAAGCTCCACCTGCTCCACAACGCCACCGATGAGACGATTGAACGGATCGATGTGACGGACGAGAAAACGCACGA
CAGCACGCTGTTCAAAACGGGCTCGTGGCTACAAGAACGGCTCGTTCTGTTCGATCGAGCGTACTTCAAGTACCGCCGCTTCGCGTTGATCGATGAGAAC
GACGGCTACTTCGTGAGTCGGCTGAAAGAAAACGCGAATCCGCTGATAACGGAGGAATTGCGGGAATGGCGCGGCCGCGCCATTCCCTTGGAGGGCAAGC
AGATCCACGATGTGGTCGATGATATCTCGCGGAAGTACATCGACGTAGAGGTCGAAGCGGAGTTCAAGCGGGGCCAGTACGAGGGAACGCGCTCGCTGGA
TACGAAGCGATTCCGCGTCGTCGGCGTCCGCGATTCGGACGCCGACGACTACCATCTCTACATTACGAATCTGCCGCGAGATGAGTTCTTCCCGGAGGAT
CTAGCAACGTTGTATCGGTGTCGTTGGGAGGTAGAAACGCTGTTTCGTGAACTGAAGACGCAGTACGAGTTGGACGAGTTCAACACGAGTGACCCGGATG
TGGTGAAAATTCTGCTGTATGCGGCGTTGCTGTCACTGCTGGTGAGTCGTGAGCTGTTGGATCTGGTCACCGAACAGGCCGATGACGAAATCGTGTTTCC
ACCGGAACGCTGGGCGGCGACCTTCCGGTCGCACGCCCAGCTCATCCTCCAGGAACTCGGTGAGTATCTCGGTTACTCGCCACCGCCGTTGCTGGAGCGG
CTGATCGAGGACGCTCAGAAGATTCACCAGCAACGACCGATCTTACAGGAGACGCTCGCTACCGCTACACAACCGAGGTGTGAGTCTTAGCTAAAGACGA
ATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1275 bp | 424 aa | 116 | 1390 | + | No |
Chemistry : DDE
ORF sequence :
MRRLTTLFPSKFLEEHAEELGVVEREGKLQIPVLVWALVFGFAAGESRTLAGFRRCYNSTADETISPGGFYHRLTPTLAEYLRDLVEHGLDEVAVPDTVD
ADIDRFRDVMIADGTVLRLHEFLSDEFQARHEEQAGAKLHLLHNATDETIERIDVTDEKTHDSTLFKTGSWLQERLVLFDRAYFKYRRFALIDENDGYFV
SRLKENANPLITEELREWRGRAIPLEGKQIHDVVDDISRKYIDVEVEAEFKRGQYEGTRSLDTKRFRVVGVRDSDADDYHLYITNLPRDEFFPEDLATLY
RCRWEVETLFRELKTQYELDEFNTSDPDVVKILLYAALLSLLVSRELLDLVTEQADDEIVFPPERWAATFRSHAQLILQELGEYLGYSPPPLLERLIEDA
QKIHQQRPILQETLATATQPRCES
ADIDRFRDVMIADGTVLRLHEFLSDEFQARHEEQAGAKLHLLHNATDETIERIDVTDEKTHDSTLFKTGSWLQERLVLFDRAYFKYRRFALIDENDGYFV
SRLKENANPLITEELREWRGRAIPLEGKQIHDVVDDISRKYIDVEVEAEFKRGQYEGTRSLDTKRFRVVGVRDSDADDYHLYITNLPRDEFFPEDLATLY
RCRWEVETLFRELKTQYELDEFNTSDPDVVKILLYAALLSLLVSRELLDLVTEQADDEIVFPPERWAATFRSHAQLILQELGEYLGYSPPPLLERLIEDA
QKIHQQRPILQETLATATQPRCES
Blast result :
Comments
One copie is present in pNRC100 of Halobacterium sp. NRC-1 at coordinates 132341-133743 (complement). 92% aa homologous with ISH8A and ISH8B.
References
1] Wailap, V. Ng., Stacy A. Ciufo, Smith, T.M., Bumgarner, R.E., Baskin, D., Faust, J., Hall, B., Loretz, C., Seto, J., Slagel, J., Hood, L., and DasSarma, S. (1998) Genome Res. 8, 1131-1141