ISSphsp5
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Sphingobium sp. | Sphingobium sp. Sphingobium sp. SA2 Sphingomonas sanxanigenens NX02 |
DNA section
IS Length : 1229 bp
Ends
IR Length : 29/31
IRL : TGGACTTGTAACGGATTTGCGCCGGTCACCTGAGCGATTAGAGCTCGCAA
IRR : TGGACTTGTAACGGTTTTGTGCCGGTCACCTATCTCATTATGCCACCTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGGCGAATTAATGCCA | TAAT | CCCCGACACACCCAATA | 4 |
| 0 |
DNA sequence
TGGACTTGTAACGGATTTGCGCCGGTCACCTGAGCGATTAGAGCTCGCAACAGGAGACCGACGAGATGATCAAGCACACAGAAGAGTTCAAGCAGGAGGC
GGTGCGGATTGCACTGACCAGCGGGTTACCCCGCGACAGGGTTGCATCGGATTTGGGGATAGGGAAGTCGACGCTGGGCAAATGGGTGTCACAGTATCGG
CCATCTGACCTGGTGGCAGCGCCGCAGGCAGATCTGGCGCGTGAGAATGAACGCCTTCGTCTTGAGAACCGTGTGCTGCGGGAGGAGAGGGAAGTGCTAA
AAAAGGCCACGCAGTTCTTCGCGAGCCAAAGGCCGTGAAGTTCGCTTTTGTGCATAGCTGGCGGCATCGTTGGCCCGTTGAACTGCTCTGCCGTGTCATG
GATGTCAGTGAACGCGGCTATCGTTCCTGGCGCTCGCGCCCGATTAGCAGGCGGGAGCGGACAGATATGAAAGTGCTGGCCCATATCCGAGAACAGTACA
GGCTGAGCCTTGGCAGCTACGGCCGTCCTCGCATGACGATGGAGTTGAAGGAGGTGGGGCTCGATGTTGGCGAGCGCCGGGTCGGGCGGCTGATGAAGAT
CAACGGGATCAAGCCAGTCCGCACGCGCAAGCACAAGGTTACGACGGATAGCCACCATCGACTGGGGGTCGCAGCGAACTGGCTGGACGGCGATTTTGCC
GCCGATGCGCCCAACCGTAAATGGGCCGGCGACATCACCTATATCTGGACGTCAGAAGGCTGGCTCTACCTTGCCGTCATTCTCGACCTGCATAGCCGTC
GCGTCGTGGGCTGGGCAGTCAGCGACAGAATGAAGAAGGACTTGGCGATCAGAGCGCTGGATATGGCGGTGCGCCTGCGCCAGCCTCCGGAAGGCTGCTT
TTTTCATTCGGATCGTGGCAGCCAATATTGTTCTTACGACTATCAAAAAAAGCTGCAGGCCTATGGCCTGCGTCCATCGATGAGCGGCAAGGGAAATTGC
TACGACAATGCGTCCGTCGAGACCTTCTTCAAATCCCTGAAGGCCGAACTCATCTGGCGGCAGAGCTGGCCGACGCGGCGTCAGGCAGAAGCCGCCATCT
TCCAGTACATCAATGGCTTCTACAACACCCGTCGTCGCCATTCATATCTGGGCGGCATCAGCCCGCTCGCGTTCGAAGCCAAGGTGGCATAATGAGATAG
GTGACCGGCACAAAACCGTTACAAGTCCA
GGTGCGGATTGCACTGACCAGCGGGTTACCCCGCGACAGGGTTGCATCGGATTTGGGGATAGGGAAGTCGACGCTGGGCAAATGGGTGTCACAGTATCGG
CCATCTGACCTGGTGGCAGCGCCGCAGGCAGATCTGGCGCGTGAGAATGAACGCCTTCGTCTTGAGAACCGTGTGCTGCGGGAGGAGAGGGAAGTGCTAA
AAAAGGCCACGCAGTTCTTCGCGAGCCAAAGGCCGTGAAGTTCGCTTTTGTGCATAGCTGGCGGCATCGTTGGCCCGTTGAACTGCTCTGCCGTGTCATG
GATGTCAGTGAACGCGGCTATCGTTCCTGGCGCTCGCGCCCGATTAGCAGGCGGGAGCGGACAGATATGAAAGTGCTGGCCCATATCCGAGAACAGTACA
GGCTGAGCCTTGGCAGCTACGGCCGTCCTCGCATGACGATGGAGTTGAAGGAGGTGGGGCTCGATGTTGGCGAGCGCCGGGTCGGGCGGCTGATGAAGAT
CAACGGGATCAAGCCAGTCCGCACGCGCAAGCACAAGGTTACGACGGATAGCCACCATCGACTGGGGGTCGCAGCGAACTGGCTGGACGGCGATTTTGCC
GCCGATGCGCCCAACCGTAAATGGGCCGGCGACATCACCTATATCTGGACGTCAGAAGGCTGGCTCTACCTTGCCGTCATTCTCGACCTGCATAGCCGTC
GCGTCGTGGGCTGGGCAGTCAGCGACAGAATGAAGAAGGACTTGGCGATCAGAGCGCTGGATATGGCGGTGCGCCTGCGCCAGCCTCCGGAAGGCTGCTT
TTTTCATTCGGATCGTGGCAGCCAATATTGTTCTTACGACTATCAAAAAAAGCTGCAGGCCTATGGCCTGCGTCCATCGATGAGCGGCAAGGGAAATTGC
TACGACAATGCGTCCGTCGAGACCTTCTTCAAATCCCTGAAGGCCGAACTCATCTGGCGGCAGAGCTGGCCGACGCGGCGTCAGGCAGAAGCCGCCATCT
TCCAGTACATCAATGGCTTCTACAACACCCGTCGTCGCCATTCATATCTGGGCGGCATCAGCCCGCTCGCGTTCGAAGCCAAGGTGGCATAATGAGATAG
GTGACCGGCACAAAACCGTTACAAGTCCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 237 bp | 78 aa | 102 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
VRIALTSGLPRDRVASDLGIGKSTLGKWVSQYRPSDLVAAPQADLARENERLRLENRVLREEREVLKKATQFFASQRP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 927 bp | 308 aa | 266 | 1192 | + | No |
Description : Second part of the transposase
ORF sequence :
EPCAAGGEGSAKKGHAVLREPKAVKFAFVHSWRHRWPVELLCRVMDVSERGYRSWRSRPISRRERTDMKVLAHIREQYRLSLGSYGRPRMTMELKEVGLD
VGERRVGRLMKINGIKPVRTRKHKVTTDSHHRLGVAANWLDGDFAADAPNRKWAGDITYIWTSEGWLYLAVILDLHSRRVVGWAVSDRMKKDLAIRALDM
AVRLRQPPEGCFFHSDRGSQYCSYDYQKKLQAYGLRPSMSGKGNCYDNASVETFFKSLKAELIWRQSWPTRRQAEAAIFQYINGFYNTRRRHSYLGGISP
LAFEAKVA
VGERRVGRLMKINGIKPVRTRKHKVTTDSHHRLGVAANWLDGDFAADAPNRKWAGDITYIWTSEGWLYLAVILDLHSRRVVGWAVSDRMKKDLAIRALDM
AVRLRQPPEGCFFHSDRGSQYCSYDYQKKLQAYGLRPSMSGKGNCYDNASVETFFKSLKAELIWRQSWPTRRQAEAAIFQYINGFYNTRRRHSYLGGISP
LAFEAKVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1091 bp | 363 aa | 102 | 1192 | + | Yes |
Chemistry : DDE
ORF sequence :
VRIALTSGLPRDRVASDLGIGKSTLGKWVSQYRPSDLVAAPQADLARENERLRLENRVLREEREVLKKGHAVLREPKAVKFAFVHSWRHRWPVELLCRVM
DVSERGYRSWRSRPISRRERTDMKVLAHIREQYRLSLGSYGRPRMTMELKEVGLDVGERRVGRLMKINGIKPVRTRKHKVTTDSHHRLGVAANWLDGDFA
ADAPNRKWAGDITYIWTSEGWLYLAVILDLHSRRVVGWAVSDRMKKDLAIRALDMAVRLRQPPEGCFFHSDRGSQYCSYDYQKKLQAYGLRPSMSGKGNC
YDNASVETFFKSLKAELIWRQSWPTRRQAEAAIFQYINGFYNTRRRHSYLGGISPLAFEAKVA
DVSERGYRSWRSRPISRRERTDMKVLAHIREQYRLSLGSYGRPRMTMELKEVGLDVGERRVGRLMKINGIKPVRTRKHKVTTDSHHRLGVAANWLDGDFA
ADAPNRKWAGDITYIWTSEGWLYLAVILDLHSRRVVGWAVSDRMKKDLAIRALDMAVRLRQPPEGCFFHSDRGSQYCSYDYQKKLQAYGLRPSMSGKGNC
YDNASVETFFKSLKAELIWRQSWPTRRQAEAAIFQYINGFYNTRRRHSYLGGISPLAFEAKVA
Blast result :
Comments
ISShsp5 is 96% aa similar to ISShsp3.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Maurizio Labbate (2021) Direct submission.