ISSphsp2

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Sphingobium sp.
Sphingobium sp.
Sphingobium sp. SA2
DNA section
IS Length : 1523 bp

Ends


IR Length : 20/26

IRL : TGTCCGCCGTCAGCACCAATGGCACAGATTTGAGGTTGTGATCTAAGGAG
IRR : TGTCCGTCGTCAGAACATTCGGCACAACTCGCGGCGTAGATTAGCGGAGT

Insertion site


Left flankDirect repeatRight flankDR Length
GCCCGGTGACGCTCGTCCTC0
TGAATCAGGCGAGCCAATGT0

DNA sequence

TGTCCGCCGTCAGCACCAATGGCACAGATTTGAGGTTGTGATCTAAGGAGGATCTGGGCTTCGTCGTAGTGACGAAGGAACGAAGATGAAGCCCAGATCC
TCCAATGTAAAATCGCCCGCCAAGGCCCCAGCAGAGCGGGTAGTGAAGGACATCCGCCGACAGACCCGGCGCCACTTTTCGGCCGAGGACAAGATCCGCA
TCGTGCTGGATGGCCTGCGCGGGGAAGACAGCATCGCCGAGCTATGCCGCAAGGAAGGCATTGCCCAGAGCCTGTATTACACCTGGTCGAAGGAGTTCAT
GGAAGCTGGCAAGCGCCGCCTGGCCGGCGACACCGCCCGTGCCGCGACCACCGGAGAGGTCCAGGACCTGCGCCGCGAAACCCGGGCCCTGAAGGAATGC
GTGGCCGATCTGACACTGGAAAACCGTCTGCTCAAAAAAAGCATGATCGCGGATGGGGGCAACGACGAATGAGGTATCCCGCATCCGAGAAGCTCGAGAT
CATCAGAACCGTCGAGCAGTCGCACCTGTCGGCCAAGCGCACGCTGGACCAACTCGGCATCCCTCGTCGGACATTCTACCGCTGGTATGATCGCTACCTC
GAAGGCGGGCCGGAGGCGTTGGAAGATCGGCCATCGGCGCCGAGCCGGGTGTGGAACCGCATCCCGGCGGGCATCCACGACCAGATCATCGAGCTGGCGC
TGGAACAGTCTGAGCTGAGCCCCCGGGAACTGGCCGTGCGCTTCACCGATGGGCAGCGCTACTTCGTGTCGGAATCCACGGTTTACCGCCTGTTGAAGGC
CCGCGACCTGATCACCAGCCCGGCCTATGTCGTGATCAAGGCTGCCGATCAGTTCCACACGAAAACCACGCGGCCGAACGAGATGTGGCAGACCGACTTC
ACCTACTTCAAGATCATCGGGTGGGGCTGGATGTATCTGTCGACCGTGCTCGACGACTTCTCGCGCTACATCATCGCCTGGAAGCTGTGCACCAACATGC
GAGCCGAGGACGTCACCGACACGCTGGACCTCGCCCTGGCGGCTTCTGGTTGCGACAGCGCCACGGTGCTGCACAAACCCCGGCTACTCAGCGATAATGG
CCCCAGCTACATCGCGGGTGAGCTGGCGGAATACATCGAGGCCCGGAAGATGAGCCATGTCCGCGGCGCCCCGTGTCATCCCCAAACCCAGGGCAAGATC
GAGCGCTGGCACCAGACCCTGAAAAACCGCATCTTGCTGGAAAACTACTTCCTGCCCGGCGACCTCGAAGCTCACATCGAGGCCTTCGTCGAGCACTACA
ATCACCAGCGATACCACGAGAGCCTGGCCAATGTGACGCCTGCCGACGCCTACTTCGGCAGGGCTCCGGCAATCATCAAACTGCGCGAAAGGATCAAGCG
ACAGACCATCGAACATCGGCGCTTGCAGCACCGCAAGTTCGCCGCCTAACATCAACCCCCTGACGAGGTCCGCACTCCGCTAATCTACGCCGCGAGTTGT
GCCGAATGTTCTGACGACGGACA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
387 bp128 aa86472+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKPRSSNVKSPAKAPAERVVKDIRRQTRRHFSAEDKIRIVLDGLRGEDSIAELCRKEGIAQSLYYTWSKEFMEAGKRRLAGDTARAATTGEVQDLRRETR
ALKECVADLTLENRLLKKSMIADGGNDE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1377 bp458 aa731449-Yes
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RRNEDEAQILQCKIARQGPSRAGSEGHPPTDPAPLFGRGQDPHRAGWPARGRQHRRAMPQGRHCPEPVLHLVEGVHGSWQAPPGRRHRPCRDHRRGPGPA
PRNPGPEGMRGRSDTGKPSAQKKHDRGWGQRRMRYPASEKLEIIRTVEQSHLSAKRTLDQLGIPRRTFYRWYDRYLEGGPEALEDRPSAPSRVWNRIPAG
IHDQIIELALEQSELSPRELAVRFTDGQRYFVSESTVYRLLKARDLITSPAYVVIKAADQFHTKTTRPNEMWQTDFTYFKIIGWGWMYLSTVLDDFSRYI
IAWKLCTNMRAEDVTDTLDLALAASGCDSATVLHKPRLLSDNGPSYIAGELAEYIEARKMSHVRGAPCHPQTQGKIERWHQTLKNRILLENYFLPGDLEA
HIEAFVEHYNHQRYHESLANVTPADAYFGRAPAIIKLRERIKRQTIEHRRLQHRKFAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1364 bp453 aa861449+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKPRSSNVKSPAKAPAERVVKDIRRQTRRHFSAEDKIRIVLDGLRGEDSIAELCRKEGIAQSLYYTWSKEFMEAGKRRLAGDTARAATTGEVQDLRRETR
ALKECVADLTLENRLLKKHDRGWGQRRMRYPASEKLEIIRTVEQSHLSAKRTLDQLGIPRRTFYRWYDRYLEGGPEALEDRPSAPSRVWNRIPAGIHDQI
IELALEQSELSPRELAVRFTDGQRYFVSESTVYRLLKARDLITSPAYVVIKAADQFHTKTTRPNEMWQTDFTYFKIIGWGWMYLSTVLDDFSRYIIAWKL
CTNMRAEDVTDTLDLALAASGCDSATVLHKPRLLSDNGPSYIAGELAEYIEARKMSHVRGAPCHPQTQGKIERWHQTLKNRILLENYFLPGDLEAHIEAF
VEHYNHQRYHESLANVTPADAYFGRAPAIIKLRERIKRQTIEHRRLQHRKFAA

 

Blast result :
Comments
ISSphsp2 is 95% aa similar to ISPko4.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Maurizio Labbate (2021) Direct submission.