ISfinder Mirror Site

ISLasa2

Family IS3
Group IS150

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP089850	ND	Lactobacillus salivarius	Lactobacillus salivarius Ligilactobacillus salivarius BNS11 Lactobacillus salivarius BNS11

DNA section

IS Length : 1619 bp

Ends

IR Length : 19/22

IRL : TGACACTTGAAATAATAAAAGTGTCAGTCCTAGGGGGTATTTTGTATTGT
IRR : TGACACTTTAAATAATGAAAATTAGCTGGCCTTGCCAAAGCGATATTCTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGTACAATACCCCTTAGGGT		ACTTGACAGGGCACAGTACC	0

DNA sequence

TGACACTTGAAATAATAAAAGTGTCAGTCCTAGGGGGTATTTTGTATTGTAAGTTATTCAACTATTGAGAAACTTAAGCTACTTAGTAAGTATCAAGAAT
CGGATTACGACCTAACGGTCTTCTCCGATTATCACGGCGTGCGTCCAGCGAATATGAGTAAATGGATCAAACAATTCTTAATGGCTGGATTAGCAGGTCT
AGTTAGGCCTAAGCATAATCGGAAATACTCATTAGAAACAAAGGTAGCCGCTGTCAAAGCTTATCTTTCAAGTGCGTATTCTAACCAGGAACTCCTTCAG
CGATACCAAATTAGGAATATTTCTCAACTCCATCAATGGGTTATCAGTTACAATAGTGGCAACCTTACCGTTAATCAGACAACGAGAAAGCGAGCTAGAA
GAATGGGACGAAAAGTAACCTTTGATGAAAAGAAGAGTATCGTTCAATGGACGATTGACCATGACAACAACTATAAGGCGGCTGCTGAGAAGTATAACGT
TAGTTATCAACGTGTTTATTCTTGGGTACGCAAGTACCAGGAAAACAATGATTGGGCAGCATTGAAGGATAACCGTGGGCGTAACAAGGGTAAAGAACCG
ACTAATGAATTAGAACGGTTAAGAAAAAAAGTTCGCGAATTAGAGGCTCGAGAGCGTGAACGGGAGGTTCAAATTGCATTTGCAAAAAAATTAGTCGAAA
TACGCAATCGGGAGGTGGATCGACCGGACGATATCAAGCAATTCAGGAAATGATAGCCGAGGGTTATTCAGTCCGTGAATTAACCAAAGCTGCTGGAGTG
TCTAGGCAAGCTTACTACAAGTGGCTTAAACATGATCCAAATGAACGTGAAATCGAGGAATTGGAACTCCTTAAGTTAATTAAGCAGTTAGAAAATGAAC
ACAAACAAAGTGTTGGTTATGACAAAATGACCAGGTTAATTAATTTGAGTCAGCAAGTTCCCTATAAGGTGAACAAGAAACGGATTATTCGTATCATGCG
AAAGCATGGTATTAAAGCTGACTATCGTCAGCCCAAACATAAGCGTATTCAGGCTCAACAAACTTATGAAGCTGAAAACATTCTTAAACGACAATTTGAT
CAAAGCGCAGCTAACCAAGTCTGGGTTACGGATACAACTGAGTTAACGTATGGGATTCGACGCAATAAGGTTCGGCTACACGTAGTATTAGATCTGTATG
GGCAATATCCAGTAAGTTGGTTAATTACGCCAACGGAAACGGCCAAAGGTGCAATCAAGGTATTTGAACAAGCCAGAGTCAGTGAAGGAGGATTAGCTCC
GTTAATCCATACGGATCGTGGTTCAGCATACATATCAAAAGCATTTAATCAATACTTGGCCATTAATGGCACATGTCATAGTTACTCTGCACCAGGTACT
CCAGCCGATAATGCAGTAATAGAACATTGGTGGGCAGATTTTAAGGCAATATGGATAGCTCACCTACCTAAAGCACAGACATTAACAGAATTAGAGCAAC
AAGTTGAAACAGGCATTACATACTTCACTGAAGAATTTATCTCGGCGAAAAGAAACGACCTTACCGCAGCAGAATATCGCTTTGGCAAGGCCAGCTAATT
TTCATTATTTAAAGTGTCA

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
705 bp	234 aa	49	753	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
998 bp	312 aa	601	1598	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TGGSNCICKKISRNTQSGGGSTGRYQAIQEMIAEGYSVRELTKAAGVSRQAYYKWLKHDPNEREIEELELLKLIKQLENEHKQSVGYDKMTRLINLSQQV
PYKVNKKRIIRIMRKHGIKADYRQPKHKRIQAQQTYEAENILKRQFDQSAANQVWVTDTTELTYGIRRNKVRLHVVLDLYGQYPVSWLITPTETAKGAIK
VFEQARVSEGGLAPLIHTDRGSAYISKAFNQYLAINGTCHSYSAPGTPADNAVIEHWWADFKAIWIAHLPKAQTLTELEQQVETGITYFTEEFISAKRND
LTAAEYRFGKAS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1550 bp	514 aa	49	1598	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VSYSTIEKLKLLSKYQESDYDLTVFSDYHGVRPANMSKWIKQFLMAGLAGLVRPKHNRKYSLETKVAAVKAYLSSAYSNQELLQRYQIRNISQLHQWVIS
YNSGNLTVNQTTRKRARRMGRKVTFDEKKSIVQWTIDHDNNYKAAAEKYNVSYQRVYSWVRKYQENNDWAALKDNRGRNKGKEPTNELERLRKKVRELEA
REREREVQIAFAISRNTQSGGGSTGRYQAIQEMIAEGYSVRELTKAAGVSRQAYYKWLKHDPNEREIEELELLKLIKQLENEHKQSVGYDKMTRLINLSQ
QVPYKVNKKRIIRIMRKHGIKADYRQPKHKRIQAQQTYEAENILKRQFDQSAANQVWVTDTTELTYGIRRNKVRLHVVLDLYGQYPVSWLITPTETAKGA
IKVFEQARVSEGGLAPLIHTDRGSAYISKAFNQYLAINGTCHSYSAPGTPADNAVIEHWWADFKAIWIAHLPKAQTLTELEQQVETGITYFTEEFISAKR
NDLTAAEYRFGKAS

Blast result :

Comments

ISLasa2 is 50% aa (transposase ORFAB) similar to ISSth1b.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] Yao Z, Wanjiang Z. (2021) Direct submission.