ISLasa1
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP089850 | ND | Lactobacillus salivarius | Lactobacillus salivarius Ligilactobacillus salivarius BNS11 |
DNA section
IS Length : 941 bp
Ends
IR Length : 23/29
IRL : AGACTTCCGCGGAAATTAAAATCCTAGTTTTCAAATTTCACCTTTGGTAT
IRR : AGACCTCCGCGGAAATAGATAGCCTATTTCTAGCAGTTCTCGAAATTATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCTGCTTGAGTATTAT | TAT | GAAATCAACGGTTCTG | 3 |
DNA sequence
AGACTTCCGCGGAAATTAAAATCCTAGTTTTCAAATTTCACCTTTGGTATGATCTTGTCAAAAAAGGTGATTTTGAATGACTGATCAAACCTATGCGGAT
CGCGTTAAAGATGCCAATGATGAACAGTTTCGCCGCATCGTTGGCATTAAACGAACCACTTTCCAAGCCATGGTGGAGTTAGTCGATGAAGACTACCGCC
AAGTCCATCAACGCCGCGGGCGACGGAGTAAATTAGATCCTGAAGCCAAGGTCTTAATTACTCTAGACTATTTGCGAAACTATGGGACGTTCCTGGAACT
CTCTTTTAAGTACGGCGTTTCAGAAACAACCGCCCGGACAATCGTTGAGCGGGTTGAGAACGTCCTAGTCAGAAGCGGAAAGTTCTCGCTTCCAAACCGG
AATGAGCTTCGCAATGCCCCGGAAAGGCCGATGACTTTCTTGATTGACGCAACAGATACCATCATCCAGCGACCTAAAAAAAACAGCGGGCGTACTACTC
AGGCAAACGCCACCGACACACCATCAAAACGCAAGTAATCCTACTTACTGATGGTAAAATCATGCGGATTGACCAGGCAAAGGGTGCGGTTCATGATTTC
CGGCTCTTTAAAGAATCCTTGGGAGCCAAGACGCACGAATCAATTCGTTTAGTAATGGATAGTGGCTATCAAGGGGTGAAAAAGATTCACTTCAATAGTT
TCACTCCGAAGAAAAAGCCGCGTGGTGGCGAGCTCTCGGATCAAGATAAGGCCTACAACCGGGCCCTATCTCGCTTTCGCATTAAGATCGAGAATGTCCA
TGCGTGGTTGAAGAAATTCAAGATCATCAGCGCTGTTTATCGTAATCGGCGGCGCCGACTTGGGCTTCGCTTTAGTTTGATCTGTGGGATTTATAATTTC
GAGAACTGCTAGAAATAGGCTATCTATTTCCGCGGAGGTCT
CGCGTTAAAGATGCCAATGATGAACAGTTTCGCCGCATCGTTGGCATTAAACGAACCACTTTCCAAGCCATGGTGGAGTTAGTCGATGAAGACTACCGCC
AAGTCCATCAACGCCGCGGGCGACGGAGTAAATTAGATCCTGAAGCCAAGGTCTTAATTACTCTAGACTATTTGCGAAACTATGGGACGTTCCTGGAACT
CTCTTTTAAGTACGGCGTTTCAGAAACAACCGCCCGGACAATCGTTGAGCGGGTTGAGAACGTCCTAGTCAGAAGCGGAAAGTTCTCGCTTCCAAACCGG
AATGAGCTTCGCAATGCCCCGGAAAGGCCGATGACTTTCTTGATTGACGCAACAGATACCATCATCCAGCGACCTAAAAAAAACAGCGGGCGTACTACTC
AGGCAAACGCCACCGACACACCATCAAAACGCAAGTAATCCTACTTACTGATGGTAAAATCATGCGGATTGACCAGGCAAAGGGTGCGGTTCATGATTTC
CGGCTCTTTAAAGAATCCTTGGGAGCCAAGACGCACGAATCAATTCGTTTAGTAATGGATAGTGGCTATCAAGGGGTGAAAAAGATTCACTTCAATAGTT
TCACTCCGAAGAAAAAGCCGCGTGGTGGCGAGCTCTCGGATCAAGATAAGGCCTACAACCGGGCCCTATCTCGCTTTCGCATTAAGATCGAGAATGTCCA
TGCGTGGTTGAAGAAATTCAAGATCATCAGCGCTGTTTATCGTAATCGGCGGCGCCGACTTGGGCTTCGCTTTAGTTTGATCTGTGGGATTTATAATTTC
GAGAACTGCTAGAAATAGGCTATCTATTTCCGCGGAGGTCT
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 462 bp | 153 aa | 77 | 538 | + | No |
Description : First part of the transposase
ORF sequence :
MTDQTYADRVKDANDEQFRRIVGIKRTTFQAMVELVDEDYRQVHQRRGRRSKLDPEAKVLITLDYLRNYGTFLELSFKYGVSETTARTIVERVENVLVRS
GKFSLPNRNELRNAPERPMTFLIDATDTIIQRPKKNSGRTTQANATDTPSKRK
GKFSLPNRNELRNAPERPMTFLIDATDTIIQRPKKNSGRTTQANATDTPSKRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 435 bp | 144 aa | 478 | 912 | + | No |
Description : Second part of the transposase
ORF sequence :
KKQRAYYSGKRHRHTIKTQVILLTDGKIMRIDQAKGAVHDFRLFKESLGAKTHESIRLVMDSGYQGVKKIHFNSFTPKKKPRGGELSDQDKAYNRALSRF
RIKIENVHAWLKKFKIISAVYRNRRRRLGLRFSLICGIYNFENC
RIKIENVHAWLKKFKIISAVYRNRRRRLGLRFSLICGIYNFENC
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 836 bp | 277 aa | 77 | 912 | + | Yes |
Chemistry : DDE
ORF sequence :
MTDQTYADRVKDANDEQFRRIVGIKRTTFQAMVELVDEDYRQVHQRRGRRSKLDPEAKVLITLDYLRNYGTFLELSFKYGVSETTARTIVERVENVLVRS
GKFSLPNRNELRNAPERPMTFLIDATDTIIQRPKKQRAYYSGKRHRHTIKTQVILLTDGKIMRIDQAKGAVHDFRLFKESLGAKTHESIRLVMDSGYQGV
KKIHFNSFTPKKKPRGGELSDQDKAYNRALSRFRIKIENVHAWLKKFKIISAVYRNRRRRLGLRFSLICGIYNFENC
GKFSLPNRNELRNAPERPMTFLIDATDTIIQRPKKQRAYYSGKRHRHTIKTQVILLTDGKIMRIDQAKGAVHDFRLFKESLGAKTHESIRLVMDSGYQGV
KKIHFNSFTPKKKPRGGELSDQDKAYNRALSRFRIKIENVHAWLKKFKIISAVYRNRRRRLGLRFSLICGIYNFENC
Blast result :
Comments
ISLasa1 is 66% aa similar (transposase ORFAB) to ISOt6.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Yao Z, Wanjiang Z.(2021) Direct submission.