ISAba61

  • Family ISL3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter baumannii
Acinetobacter baumannii
DNA section
IS Length : 2660 bp

Ends


IR Length : 16/23

IRL : GGTTCTTCGCAGATTCGAGTGAAGCCTGCTATGATACGAGCCCATTCACG
IRR : GGTTCATCGCACTTTGCCGGGAAACGCAGCACGAATCTTTAGGAAGAAGT

Insertion site


Left flankDirect repeatRight flankDR Length
TTGATTCACACCCATTGAAAATAGCGTGATTACCTTTTCAG8

DNA sequence

GGTTCTTCGCAGATTCGAGTGAAGCCTGCTATGATACGAGCCCATTCACGAGTTAAGAAGCACATGGGCATGTCCAAAAAAGCAGACAAGTAAGCCGCAA
CAACCTTCGTTTTTCAGTTGTTGCGGCGTTCTCATACCCTAATCTTTGCTCTGCGAGAGCGCCGCCAAATCCTTAGCTCAAGGAGACGGTTTCGTGCGCT
CACAAAATTATTCCTGGCGGTATTCTCTTACCGCCACTGTGCTGTTGTTATCACCATTCGACTTGTTGGCATCACTTGGCATGGACATGTATTTGCCTGT
GGTGCCTTTCATGCCCGGTGCGCTTGGCACCACCGCGGAGACAATCCAGCTTACGTTAACAACATACCTGATCATGATAGGAGCTGGTCAGCTTCTATTT
GGACCATTGTCAGACCGGCTGGGACGTCGTCCCGTTTTACTTGCTGGCGGCGTTGCCTACATTGTGGCCTCTATTGGCCTCGCAGTAACATCGTCAGCCG
AACTGTTCCTGAGTCTTCGAATCGTTCAAGCTTGCGGTGCCTCGGCGTGTCTCGTTGCCACGTTTGCAACAGTGCGCGACATCTACGCCGGCCGCGATGA
AAGTAACGTTATTTATGGCTTGCTCAGTTCCATGCTGGCTATAGTGCCTGCGGTAGGGCCATTGCTGGGAACGCTCGTCGACACTTGGCTGGGATGGCGT
GCAATTTTCGTCTTGCTGGGAATCGGGATGATGGTCTCCTGTACGGCTGCGTGGAGACTCTGGCCCGAAACTCGGAGGCAACGAACGCCGGACTTTCAGT
GGTCGCAGCTATTACTCCCAATGAAGCGATTGAACTTCTGGCTGTATACGCTTTGTTACAGCGCGGGAATGGGTAGCTTCTTTGTCTTTTTCTCGACCGC
CCCTCGGTTAATGATGGGCAGGCAAGAATTGTCGCAGCTCAGTTTCAGTTTGCTATTTGCAACAGTGGCCATCGCAATGATGGGTACAGCGAGAATCATG
GGGCGTTTGATTCCCCGGTGGGGTAGCCTGAACACCTTGCGGATAGGAATGGGATGCCTGATGGCAGGGGCAATTTTCCTCGTTATGAGTGAATTATGGG
CACCGTTGTCGGTGCTTGGCTTCATCGCCCCAATGTGGCTCGTGGGGGTAGGTGTTGCCACTGCGGTGTCTGTGGCACCCAATGGCGCGCTCCGGGGGTT
CGATCATGTTGCCGGAGCCGTTACAGCAGTCTACTTCTGTTTGGGTGGGCTACTGCTAGGGGGCATCGGGACGCTCATCATCGCACTTTTACCGAGTGAT
ACTGCTTGGCCGGTCATCGCTTATTGCATGATCCTCGCAACAGTCGTACTTGGTCTATCCTGCATGAACTCCGCGAGACACCGTCGTGGCAATGAAGTAC
ACGATACCGTGGTGCTGCAAGGTGCCGATTGCGAGCAAGAGGAACATGGCCATGACTAGCGCGAGCGTCTGGAATTTATTCTGGAAAGGCTTTGCGCCGG
CGTCCCATGAGCTTCTGGACGAACGGACCCTGCTCATCCGTCTTAGACACGATGAGGAGCATTCGCCAGTATGTGGCCGATGCGGCCGACCTTGCTTTCT
GGTACACGACGTTAATTGGCGTCGTGTTCGTGAGTCACCGATTATGCAGTATAGAGTCGAACTGGAGGTACCCGTTCGACGGCTGCGTTGCTCCCGGTGC
GGCCCATCCAGAGAGCGGATTGATTGGCTACCAGGGCGATCCCGTATTACGCAAACATTGCGCCATTGGGTTGAGAATCTGGTACGGATGCTGCCTATCA
GCCATGTTGCCAAACTCTTAGGGCTGAACTGGCATACAGTCAAGCGAATCGACATGGAGCGGCTGAAACGTGATTTATCCGAGCCAGATCGAGGCCGGCT
ACGCCGTTTAATGATGGACGAATTCGCCCTGCACAAGGGGCATCGCTACGCCACCGTCGTCGCCTGTGCCGATACTCAGCAGGTGGTATGGATCGGCGAG
GGGCGTTCCCGTGAGGCGATCCGCCCTTTCTTCGAGTGGTTGGGTGATGCTCGGGAGCAGATCGAGGCAGTGGCCATGGACATGAACTCCGCCTTCGACC
TGGAGGTGAAGGAGCAGTGTGATAACGCGGTGGTGGTCTATGACCTCTTCCATGTGGTGGCCAAGTACGGGCGCGAGGTCGTGGATCGGGTGCGCGTCGA
TCAGGCCAACCAGTTGCGGGACGACAAGGCCGCCCGCAAGGTCATCAAGAGCAGCCGCTGGCTACTGCTGCGCAATGCTGACAACTTGAAGCCCGAACAG
GCGGTCAAGCTCGAAGAGCTCCTGGCCGCCAATGCCTCATTGGCCACGGTTTACGTGCTCAAGGATCAGCTCAAGACGCTCTGGTTCGCCCGGGACGAGG
CCCGCGCCCGAAACGCCTGGCAGGAGTGGGCGGGCATGGCCCTCAGCAGCGGCATTGACGCCTTGGAGCGCTTCGCCGGGAGGCTGGAGCCCTACGTGGA
AGGAATCGTCTCCAGCGCCCAGCACCGGCTAAATACCAGCGTGTTGGAAGGCATGAACAACCGGATCAAGGTCATCAAGCGGATGGCTTATGGGTATCGC
GACACGGCTTACTTCTTCCTAAAGATTCGTGCTGCGTTTCCCGGCAAAGTGCGATGAACC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1179 bp392 aa2811459+No
ORF function : Passenger Gene
Annotation : CmlA/FloR family chloramphenicol efflux MFS transporterDescription : Antibiotic resistance

ORF sequence :

MDMYLPVVPFMPGALGTTAETIQLTLTTYLIMIGAGQLLFGPLSDRLGRRPVLLAGGVAYIVASIGLAVTSSAELFLSLRIVQACGASACLVATFATVRD
IYAGRDESNVIYGLLSSMLAIVPAVGPLLGTLVDTWLGWRAIFVLLGIGMMVSCTAAWRLWPETRRQRTPDFQWSQLLLPMKRLNFWLYTLCYSAGMGSF
FVFFSTAPRLMMGRQELSQLSFSLLFATVAIAMMGTARIMGRLIPRWGSLNTLRIGMGCLMAGAIFLVMSELWAPLSVLGFIAPMWLVGVGVATAVSVAP
NGALRGFDHVAGAVTAVYFCLGGLLLGGIGTLIIALLPSDTAWPVIAYCMILATVVLGLSCMNSARHRRGNEVHDTVVLQGADCEQEEHGHD

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1206 bp401 aa14522657+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MTSASVWNLFWKGFAPASHELLDERTLLIRLRHDEEHSPVCGRCGRPCFLVHDVNWRRVRESPIMQYRVELEVPVRRLRCSRCGPSRERIDWLPGRSRIT
QTLRHWVENLVRMLPISHVAKLLGLNWHTVKRIDMERLKRDLSEPDRGRLRRLMMDEFALHKGHRYATVVACADTQQVVWIGEGRSREAIRPFFEWLGDA
REQIEAVAMDMNSAFDLEVKEQCDNAVVVYDLFHVVAKYGREVVDRVRVDQANQLRDDKAARKVIKSSRWLLLRNADNLKPEQAVKLEELLAANASLATV
YVLKDQLKTLWFARDEARARNAWQEWAGMALSSGIDALERFAGRLEPYVEGIVSSAQHRLNTSVLEGMNNRIKVIKRMAYGYRDTAYFFLKIRAAFPGKV
R

 

Blast result :
Comments
ISAba61 is 93% aa similar to ISAba43.
References
1] Samira Mohamed Hamed (2021) Direct submission.