ISPa157
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP015003.1 | Y | Pseudomonas aeruginosa | Pseudomonas aeruginosa P-10.226 Pseudomonas aeruginosa PA11803 Pseudomonas aeruginosa PA7790 Pseudomonas aeruginosa CCBH4851 Pseudomonas aeruginosa PA8281 |
DNA section
IS Length : 1308 bp
Ends
IR Length : 26/32
IRL : TGGGGTGTGATGATTCTTGTTGACGGGGAAACCCGTAACCGACAAGGATT
IRR : TGGGCTGTGTCAAGTTTTGTTGACGGTGAAACTCTTTAGTTTGTTGGTTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| aagtatctgtccagaccgcttgccattgaa | gaa | gaaggcatgttagagtttggggcatgtcga | 3 |
DNA sequence
TGGGGTGTGATGATTCTTGTTGACGGGGAAACCCGTAACCGACAAGGATTGAGAGTATGCCAAAGGCTTATCCACCACAGTTCAAGCAGCAATGCGTTGA
TGCAGTGCTAGTGCTAGGAAAGACTCGGGAAGAAGTGGCCCGGGAATATGAGGTCTCAGCATCAGCGTTAGGCCGCTGGGTAGCTGCTGCACAAGGCACG
CAAGGTACCGGCTCCGGCTCGCACCTACGCAAAGCCGATCCCAACTCGCAGGATTCGGCCGAGTTATCTAAACGAGTTCGTGAACTGGAACTGGAGAACG
AGTTTTTAAAAAAAGCGACCGCCTTCTTCGCCAAGGGCATGTAGACGAGCATTCCTTTGCCCTGATCCGTCATTTTGAAGAAGAAGGCACATTTACGGTG
ACTATGATGGCCCGACTTCTACGCGTGAGCCGTGCCGGCTACTACGGGTGGAAAAAGCGCCGAAACCGGAATTATCCTTTATCTACTCCACGAGGATTTC
GTGACCAGATTGCCGACGACGTCAAAGAAATCTTCGCCAAGCATAATGGTTTCGCTGGCGTACGTACCATCGCCACAGAACTAGGCACGCGTGGTATTAC
AGCCACGTTGTATGCCGTGCGTAAAACCATGCGGAAGCTAGGGCTAGTAACTAAGTATCGCCGAGCGTTTAAACGCACGACCATTACCGATGCCAACGCG
CGAGAGCGCAGCGATCTTGTACGACGCAATTTCACTCCACCAGTTCCAACAACGTACTTGTGCGGTGATATTACCTACCTTCGTACCGGGCAGGGTTGGA
TGTATTTAGCGACAGTGATTGATTTATCCACCAGGATGATCACAGGCTGGCAGGTAGCGGATCGTATGACCAGCCAGCTGGTCATTGACGCACTGGACAT
GGCTCATCGCAGTGGCTATGTCGCTGGTAATGCCATATTTCATTCCGATCGCGGCGCCCAGTACACCAGTGCGGCGTTAAGTCGGTGGGCCGAGAACCAT
GATGTTCGCTTATCAGTCGGCGAGGTCGGCGTGTGCTGGGATAACGCTGTGGCGGAATCGTTCTTTTCCACCCTGAAACTGCATTTACTCTACGAGCGGA
AACAGTTCGTTTCCAAACTCGAAGCGCGAATCAGCGTAGGCGAATGGATCGAGGCCTACTACAACCGCCGACGCATCCACACCAGCACCGAAGAAATCCC
TAAGAAAGCAATGGACGACTTCCTGACCACACCTGTGACTACCGCCGCGCAAGCGGCCTAACCAACAAACTAAAGAGTTTCACCGTCAACAAAACTTGAC
ACAGCCCA
TGCAGTGCTAGTGCTAGGAAAGACTCGGGAAGAAGTGGCCCGGGAATATGAGGTCTCAGCATCAGCGTTAGGCCGCTGGGTAGCTGCTGCACAAGGCACG
CAAGGTACCGGCTCCGGCTCGCACCTACGCAAAGCCGATCCCAACTCGCAGGATTCGGCCGAGTTATCTAAACGAGTTCGTGAACTGGAACTGGAGAACG
AGTTTTTAAAAAAAGCGACCGCCTTCTTCGCCAAGGGCATGTAGACGAGCATTCCTTTGCCCTGATCCGTCATTTTGAAGAAGAAGGCACATTTACGGTG
ACTATGATGGCCCGACTTCTACGCGTGAGCCGTGCCGGCTACTACGGGTGGAAAAAGCGCCGAAACCGGAATTATCCTTTATCTACTCCACGAGGATTTC
GTGACCAGATTGCCGACGACGTCAAAGAAATCTTCGCCAAGCATAATGGTTTCGCTGGCGTACGTACCATCGCCACAGAACTAGGCACGCGTGGTATTAC
AGCCACGTTGTATGCCGTGCGTAAAACCATGCGGAAGCTAGGGCTAGTAACTAAGTATCGCCGAGCGTTTAAACGCACGACCATTACCGATGCCAACGCG
CGAGAGCGCAGCGATCTTGTACGACGCAATTTCACTCCACCAGTTCCAACAACGTACTTGTGCGGTGATATTACCTACCTTCGTACCGGGCAGGGTTGGA
TGTATTTAGCGACAGTGATTGATTTATCCACCAGGATGATCACAGGCTGGCAGGTAGCGGATCGTATGACCAGCCAGCTGGTCATTGACGCACTGGACAT
GGCTCATCGCAGTGGCTATGTCGCTGGTAATGCCATATTTCATTCCGATCGCGGCGCCCAGTACACCAGTGCGGCGTTAAGTCGGTGGGCCGAGAACCAT
GATGTTCGCTTATCAGTCGGCGAGGTCGGCGTGTGCTGGGATAACGCTGTGGCGGAATCGTTCTTTTCCACCCTGAAACTGCATTTACTCTACGAGCGGA
AACAGTTCGTTTCCAAACTCGAAGCGCGAATCAGCGTAGGCGAATGGATCGAGGCCTACTACAACCGCCGACGCATCCACACCAGCACCGAAGAAATCCC
TAAGAAAGCAATGGACGACTTCCTGACCACACCTGTGACTACCGCCGCGCAAGCGGCCTAACCAACAAACTAAAGAGTTTCACCGTCAACAAAACTTGAC
ACAGCCCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 288 bp | 95 aa | 57 | 344 | + | No |
Description : First part of the transposase
ORF sequence :
MPKAYPPQFKQQCVDAVLVLGKTREEVAREYEVSASALGRWVAAAQGTQGTGSGSHLRKADPNSQDSAELSKRVRELELENEFLKKATAFFAKGM
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 978 bp | 325 aa | 284 | 1261 | + | No |
Description : Second part of the transposase
ORF sequence :
TGTGERVFKKSDRLLRQGHVDEHSFALIRHFEEEGTFTVTMMARLLRVSRAGYYGWKKRRNRNYPLSTPRGFRDQIADDVKEIFAKHNGFAGVRTIATEL
GTRGITATLYAVRKTMRKLGLVTKYRRAFKRTTITDANARERSDLVRRNFTPPVPTTYLCGDITYLRTGQGWMYLATVIDLSTRMITGWQVADRMTSQLV
IDALDMAHRSGYVAGNAIFHSDRGAQYTSAALSRWAENHDVRLSVGEVGVCWDNAVAESFFSTLKLHLLYERKQFVSKLEARISVGEWIEAYYNRRRIHT
STEEIPKKAMDDFLTTPVTTAAQAA
GTRGITATLYAVRKTMRKLGLVTKYRRAFKRTTITDANARERSDLVRRNFTPPVPTTYLCGDITYLRTGQGWMYLATVIDLSTRMITGWQVADRMTSQLV
IDALDMAHRSGYVAGNAIFHSDRGAQYTSAALSRWAENHDVRLSVGEVGVCWDNAVAESFFSTLKLHLLYERKQFVSKLEARISVGEWIEAYYNRRRIHT
STEEIPKKAMDDFLTTPVTTAAQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1205 bp | 401 aa | 57 | 1261 | + | Yes |
Chemistry : DDE
ORF sequence :
MPKAYPPQFKQQCVDAVLVLGKTREEVAREYEVSASALGRWVAAAQGTQGTGSGSHLRKADPNSQDSAELSKRVRELELENEFLKKSDRLLRQGHVDEHS
FALIRHFEEEGTFTVTMMARLLRVSRAGYYGWKKRRNRNYPLSTPRGFRDQIADDVKEIFAKHNGFAGVRTIATELGTRGITATLYAVRKTMRKLGLVTK
YRRAFKRTTITDANARERSDLVRRNFTPPVPTTYLCGDITYLRTGQGWMYLATVIDLSTRMITGWQVADRMTSQLVIDALDMAHRSGYVAGNAIFHSDRG
AQYTSAALSRWAENHDVRLSVGEVGVCWDNAVAESFFSTLKLHLLYERKQFVSKLEARISVGEWIEAYYNRRRIHTSTEEIPKKAMDDFLTTPVTTAAQA
A
FALIRHFEEEGTFTVTMMARLLRVSRAGYYGWKKRRNRNYPLSTPRGFRDQIADDVKEIFAKHNGFAGVRTIATELGTRGITATLYAVRKTMRKLGLVTK
YRRAFKRTTITDANARERSDLVRRNFTPPVPTTYLCGDITYLRTGQGWMYLATVIDLSTRMITGWQVADRMTSQLVIDALDMAHRSGYVAGNAIFHSDRG
AQYTSAALSRWAENHDVRLSVGEVGVCWDNAVAESFFSTLKLHLLYERKQFVSKLEARISVGEWIEAYYNRRRIHTSTEEIPKKAMDDFLTTPVTTAAQA
A
Blast result :
Comments
ISPa157 is 60% aa similar to ISGmo1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
This putative IS-like element was found disrupting a IS5564 that, in turn, was truncated to an orf5 (-13 bp) of a new class 1 integron.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
This putative IS-like element was found disrupting a IS5564 that, in turn, was truncated to an orf5 (-13 bp) of a new class 1 integron.
References