ISH50
- Family IS1595
- Group ISH4
Isoform Synonym(s) ISH4
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X01584 | Y | Halobacterium salinarum | Halobacterium salinarum PHH1 Halobacterium tunesiensis Halobacterium salinarum NRC-1 plasmid pNRC100 Halobacterium salinarum R1 plasmid pHH1 |
DNA section
IS Length : 996 bp
Ends
IR Length : 23/29
IRL : CGCTCTTGGGTAGAGGATTGTTGGTCAGTTCTCAGCAGTAGCTGGAAGTC
IRR : CGCTCTTGTGGCGGAGATTGTTGGTGAGTCATAGCGCAGTCTCAAGGATC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCGTAGTAT | TTGTGGAT | AATTCAACTC | 8 |
DNA sequence
CGCTCTTGGGTAGAGGATTGTTGGTCAGTTCTCAGCAGTAGCTGGAAGTCACAGCAGGCAAGGGTGACGCGGCCGGCTCAGACGAACGATGATGCCAATC
AAGACATTCGTCTCGGAGCGTCGCGCCGCGAATCTGCTAGCACAGATACGCTGCGTAACGCGGTCTATTGCCGCGAGCTGCCGTGCCGAACTCTGTGACT
TCGGTACGGGCACGTATCGCGTGTTTCAGCGGTATCTGTGTAAGGATTGCGACCGCACGTTCAACGATCAGACTGGCACCGTCTTCGAACACTCTGCGGT
CGCGCTCAGAAAGTGGTTTCTCGCCGTCTACACCTACATCCGCTTAAACACGAGTATCAGGCAGTTAGACGCAGAGATCGACGTTTCCTACAAGACGGTC
TACCGGTCACGGCGCTTCCTGCGACGCGTGGACGCGCCTCGACCACACCTCGAAGGCCCCGTTGAGATCGACGAATTCTACGTTGAAAGCGGGCCTCAAG
GGCGCGAGCGCGACCAACCGTCGCGTGCGGGACTGTCCACGGCGGACGTTGGAACATACGCTGAGGATAAGCTCCCTGTGTTTGTTCTCGCAGATCGTGG
CACCGGAGAACGGCACGTGATCCCGGCGAAAGCCGCGACCGAATCGCGGATTCGACTCCTGCTGGCCGACCGACAGCAGGAGTCGTTAACCGTCTACACA
GACGGCTTTCGGGCGTACGATCCACTTGACGAGGACGACGCTTTCACCCGTGAATACGTCGTCCACGGTGACGGCGAGTACGTCAATGGAGACGTTCATG
TGAACACCTGCGAGAGCACGCGTCGCTGGCGCGACGGTGGCTCTCGCCGCACCGAGGCGTCTCTAAAGACAGACTCACACCATATCTCCGGCGTTTCAGC
TCCGACGTGAAGTGTTCCGAAAACCGGGGAAAGAAGCGCTCAAAACGATCCTTGAGACTGCGCTATGACTCACCAACAATCTCCGCCACAAGAGCG
AAGACATTCGTCTCGGAGCGTCGCGCCGCGAATCTGCTAGCACAGATACGCTGCGTAACGCGGTCTATTGCCGCGAGCTGCCGTGCCGAACTCTGTGACT
TCGGTACGGGCACGTATCGCGTGTTTCAGCGGTATCTGTGTAAGGATTGCGACCGCACGTTCAACGATCAGACTGGCACCGTCTTCGAACACTCTGCGGT
CGCGCTCAGAAAGTGGTTTCTCGCCGTCTACACCTACATCCGCTTAAACACGAGTATCAGGCAGTTAGACGCAGAGATCGACGTTTCCTACAAGACGGTC
TACCGGTCACGGCGCTTCCTGCGACGCGTGGACGCGCCTCGACCACACCTCGAAGGCCCCGTTGAGATCGACGAATTCTACGTTGAAAGCGGGCCTCAAG
GGCGCGAGCGCGACCAACCGTCGCGTGCGGGACTGTCCACGGCGGACGTTGGAACATACGCTGAGGATAAGCTCCCTGTGTTTGTTCTCGCAGATCGTGG
CACCGGAGAACGGCACGTGATCCCGGCGAAAGCCGCGACCGAATCGCGGATTCGACTCCTGCTGGCCGACCGACAGCAGGAGTCGTTAACCGTCTACACA
GACGGCTTTCGGGCGTACGATCCACTTGACGAGGACGACGCTTTCACCCGTGAATACGTCGTCCACGGTGACGGCGAGTACGTCAATGGAGACGTTCATG
TGAACACCTGCGAGAGCACGCGTCGCTGGCGCGACGGTGGCTCTCGCCGCACCGAGGCGTCTCTAAAGACAGACTCACACCATATCTCCGGCGTTTCAGC
TCCGACGTGAAGTGTTCCGAAAACCGGGGAAAGAAGCGCTCAAAACGATCCTTGAGACTGCGCTATGACTCACCAACAATCTCCGCCACAAGAGCG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 822 bp | 273 aa | 89 | 910 | + | No |
Chemistry : DDE
ORF sequence :
MMPIKTFVSERRAANLLAQIRCVTRSIAASCRAELCDFGTGTYRVFQRYLCKDCDRTFNDQTGTVFEHSAVALRKWFLAVYTYIRLNTSIRQLDAEIDVS
YKTVYRSRRFLRRVDAPRPHLEGPVEIDEFYVESGPQGRERDQPSRAGLSTADVGTYAEDKLPVFVLADRGTGERHVIPAKAATESRIRLLLADRQQESL
TVYTDGFRAYDPLDEDDAFTREYVVHGDGEYVNGDVHVNTCESTRRWRDGGSRRTEASLKTDSHHISGVSAPT
YKTVYRSRRFLRRVDAPRPHLEGPVEIDEFYVESGPQGRERDQPSRAGLSTADVGTYAEDKLPVFVLADRGTGERHVIPAKAATESRIRLLLADRQQESL
TVYTDGFRAYDPLDEDDAFTREYVVHGDGEYVNGDVHVNTCESTRRWRDGGSRRTEASLKTDSHHISGVSAPT
Blast result :
Comments
ISH50 has been isolated through its inactivation of the gvp operon (gas vesicle proteins, Vac-). ISH50 is extensively homologous (but not identical) to ISH23 (Pfeifer et al., 1984): although two restriction sites are different between the two elements, their first and last 200 bp were found to identical (Pfeifer, 1993). There are 2 ISH23/ISH50 copies in the Halobacterium salinarum NRC817 chromosome (Pfeifer et al., 1986).
96% DNA and 86% aa homologous with ISH50 (ISH4 is longer than ISH50)
96% DNA and 86% aa homologous with ISH50 (ISH4 is longer than ISH50)
References
1] Sapienza, C., Rose, C., and Doolittle, W. F. (1982) Nature 299, 182-185
2] Xu, W.-L., and Doolittle, W. F. (1983) Nucl. Acids Res. 11, 4195-4199.
3] Pfeifer, F., Friedman, J., Boyer, H.W., and Betlach, M. (1984) Nucl. Acids Res. 12, 2489-2497.
4] DasSarma, S. (1989) Can. J. Microbiol. 35, 65-72.
5] Ng, W.-L., Kothakota, S., and DasSarma, S. (1991) J. Bacteriol. 173, 1958-1964.
6] Pfeifer, F. (1993) Personal communication.
2] Xu, W.-L., and Doolittle, W. F. (1983) Nucl. Acids Res. 11, 4195-4199.
3] Pfeifer, F., Friedman, J., Boyer, H.W., and Betlach, M. (1984) Nucl. Acids Res. 12, 2489-2497.
4] DasSarma, S. (1989) Can. J. Microbiol. 35, 65-72.
5] Ng, W.-L., Kothakota, S., and DasSarma, S. (1991) J. Bacteriol. 173, 1958-1964.
6] Pfeifer, F. (1993) Personal communication.