ISfinder Mirror Site

ISPa120

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP047592.1	ND	Pseudomonas aeruginosa	Pseudomonas aeruginosa INP-43

DNA section

IS Length : 2880 bp

Ends

IR Length : 20/24

IRL : GTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGG
IRR : GTAAGCGTACGGCCAATACACCTTTACTTAGGTTGAGCCGTATCAGCAAA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GACTCGTAGTCT	CCTGAAAA	GACCGGCGGCTGGC	8

DNA sequence

GTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCC
ATGCGCCAACGAAGCTCTTACCCGAAACCGTTCAAAGCCCAGGTCGTTCAGGAATGCCTGCAACCTGGGGCAACGGTGTCCAGTGTCGCCATCAGCCACG
GCATCAACGCCAATGTCATCCGCAAATGGCTGACGCTTTATCGAGACCAGCCCGTACCAGCCTCGTTACCAGCCTTTGTCCCGCTGAAGGCCACCCCTAA
ACGGCCAGCCGAAACGTCAGTGCTCATTGAACTGCCCATGGCCGGGCAAATGATCACGGTGAAATGGCCAGCCTCAGATCCCGAGGGCTGCGCGCAATTC
ATCCGGGCTTTCGCTCGATGATCCGCATCGATGCGATCTGGCTAGCCACCGAACCGATGGACATGCGCGCCGGCACCGAGACGGCATTAGCCCGGGTAAT
TGCGGTGTTCGGTGCGGCGAAGCCGCACTGCGCTTATCTGTTCGCCAATCGCCGGGCTAACCGAATGAAAGTGCTGGTGCACGATGGCGTGGGCATCTGG
CTTGCCGCGCGTCGACTGAACCAAGGCAAGTTCCACTGGCCCGGCATTCGCCATGGCTGCGAGGTCGAACTCGACAGCGAACAACTCCAGGCCTTGGTGC
TGGGCCTGCCGTGGCAGCGCGTCGGCACAGGCGGTGTGATCAGCATGCTGTAAACGCCGGCCATTTGGCTGGTCTACTTGTCGTCGCATCGCGCCTGCTC
TGGCACAATCGGCGGCATGACTTCCTCGCCCAACCTTGACCAGATGACCCCGGAACAGCTTCGTGCCTTGGCGGCACAGGCGTTGCAGTTGCAATCCCAG
GTCGAGGCGATGAGCAGGAAAATCCGCAACAATGAAACCCTCATCGAACAGTTCAAGTTCGAAATCGCTCTGCTCAAACGCCACAAGTTTGCCAAGCGCA
GCGAGCAAATCAGTTCGGCGCAAGGCAGCTTGCTGGATGACCTGCTCGACACCGACCTTGAAGCTATCGAGGCCGAGCTGAAACAACTCCTTCCAGCTTC
GCCACAAGCCGAGCCACGGCAATCCCCGAAACGTTCGCCATTGCCGCCGCAGTTCCCGCGCACGGTGATTCGCCACGAACCTGAAAATACCCAATGCGCC
TGCGGCTGCCAACTTCAACGCATCGGCGAAGACGTCAGCGAGAAGCTGGATTACACGCCGGGCGTGTTTACCGTCGAGCAACATGTGAGGGGCAAATGGG
CCTGCCGTCAGTGCGAAACCCTGATCCAGGCGCCGGTGCCAGCCCAGGTTATTGATAAAGGCATCCCGACCGCAGGTTTGTTGGCCCACGTGATGGTGGC
CAAGTTTGCCGATCACTTGCCGCTGTACAGACAGGAAAAAATCTTTGGCCGCGCCGGGCTGCCAATTGCCCGCTCGACCCTGGCGCAGTGGGTCGGACAA
ACTGGCGTGCGGCTTCAGCCACTGGTCGATGCACTGCGTGAAGCCGTGCTGAACCAGGACGTGATCCACGCCGATGAAACACCGGTGCAAATGCTTGCAC
CAGGCGAGAAGAAAACCCACCGGGTCTATGTCTGGGCCTACAGCACGACGCCGTTTTCGGCGCTCAAAGCGGTGGTTTACGACTTCAGCCCAAGCCGTGC
CGGAGAACATGCACGCAACTTCCTAGGCGACTGGAATGGCAAGCTGGTCTGCGACGACTTCGCTGGATACAAGGCCGGTTTTGAACAAGGCATCACTGAA
ATCGGCTGCATGGCTCATGCTCGCCGCAAGTTCTTCGACCTGCATGTCGCTAACAAAAGCCAACTGGCCGAACAGGCGCTGCACTCAATTGGCGGTTTGT
ACGAGGTTGAACGCCAGGCTCGGGACATGAGCAACGAAGACCGTTGGCGAATACGTCAGGAAATGGCGGTACCGATCAGCAAAACACTGCATGACTGGAT
GTTGGCCCAGCGCGACCTGGTGCCCAACGGCTCGGCCACAGCTAAAGCCCTCGACTACAGCCTGAAACGCTGGGGAGCGCTGACGCGCTACCTGGACGAT
GGGGCTGTGCCCATCGACAACAATCAGGTGGAGAACCAGATACGGCCGTGGGCGCTCGGACGCTCGAACTGGTTATTTGCCGGATCGCTGCGCAGTGGCA
AACGAGCAGCAGCTATCATGAGCCTGATCCAGTCCGCTCGCATGAACGGGCATGATCCGTATGCCTACCTGAAGGACGTGCTAACTCGCCTGCCGACGTT
ACGGTCGAAAGACATCAGCCAGTTGCTGCCGCATCAGTGGGTACAGATCTAGCTTATGTGATCTATTGTCCCCTGTGAAACATATATAAATCATACTTGG
TAGGTGAGGGAAATGGATATTCGCCTGGAGATTTTAGCGCTTGAACAGCTGTTGCTAGAGCCGGAATCGAGAAAGAATGATCGACTGCTTAAACAGCTGC
TTACCGAAGACTTCGTTGAATTTGGAGCTATCGGCAAAAGCTGGACGAAAGCGGAGGTGATCGTGGGACTAAAATCCCAGACTTGGATCAAAAGGACAAT
CGAGGATTTCAAACTGCGTGTGCTTGCAGATGGTGTCGCGTTAGCAACGTACCGATGCCGTCATCAAAATGCTAATGGCGATGAGTCGTTATCAATGCGT
AGCTCTGTTTGGAAAACCTACGAAGATGGTTGGCACATGGTGTTTCACCAAGGCACGAGGGTCTCCGAGTAGATGTCGGTACCAAAGCCAATGTGCATGA
GGCGGTACCTCACACATATGAATCGATTCTTTTGCTGATACGGCTCAACCTAAGTAAAGGTGTATTGGCCGTACGCTTAC

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
321 bp	106 aa	101	421	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MRQRSSYPKPFKAQVVQECLQPGATVSSVAISHGINANVIRKWLTLYRDQPVPASLPAFVPLKATPKRPAETSVLIELPMAGQMITVKWPASDPEGCAQF
IRAFAR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
336 bp	111 aa	418	753	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIRIDAIWLATEPMDMRAGTETALARVIAVFGAAKPHCAYLFANRRANRMKVLVHDGVGIWLAARRLNQGKFHWPGIRHGCEVELDSEQLQALVLGLPWQ
RVGTGGVISML

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1536 bp	511 aa	817	2352	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTSSPNLDQMTPEQLRALAAQALQLQSQVEAMSRKIRNNETLIEQFKFEIALLKRHKFAKRSEQISSAQGSLLDDLLDTDLEAIEAELKQLLPASPQAEP
RQSPKRSPLPPQFPRTVIRHEPENTQCACGCQLQRIGEDVSEKLDYTPGVFTVEQHVRGKWACRQCETLIQAPVPAQVIDKGIPTAGLLAHVMVAKFADH
LPLYRQEKIFGRAGLPIARSTLAQWVGQTGVRLQPLVDALREAVLNQDVIHADETPVQMLAPGEKKTHRVYVWAYSTTPFSALKAVVYDFSPSRAGEHAR
NFLGDWNGKLVCDDFAGYKAGFEQGITEIGCMAHARRKFFDLHVANKSQLAEQALHSIGGLYEVERQARDMSNEDRWRIRQEMAVPISKTLHDWMLAQRD
LVPNGSATAKALDYSLKRWGALTRYLDDGAVPIDNNQVENQIRPWALGRSNWLFAGSLRSGKRAAAIMSLIQSARMNGHDPYAYLKDVLTRLPTLRSKDI
SQLLPHQWVQI

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
360 bp	119 aa	2413	2772	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDIRLEILALEQLLLEPESRKNDRLLKQLLTEDFVEFGAIGKSWTKAEVIVGLKSQTWIKRTIEDFKLRVLADGVALATYRCRHQNANGDESLSMRSSVW
KTYEDGWHMVFHQGTRVSE

Blast result :

Comments

ISPa120 is 97% (transposase) aa similar to ISPpu14

References

1] Dongsheng Zhou (2020) Direct submission.
2] Garcia-Contreras,R., Perez-Eretza,B., Jasso-Chavez,R., Lira-Silva,E., Roldan-Sanchez,J.A., Gonzalez-Valdez,A., Soberon-Chavez,G., Coria-Jimenez,R., Martinez-Vazquez,M., Alcaraz,L.D., Maeda,T. and Wood,T.K. (2015) Pathog Dis 73 (6), ftv040