ISLgr1
- Family IS3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AL592102.1 | ND | Listeria grayi | Listeria grayi Listeria grayi 19/09/S Listeria innocua Clip11262 plasmid pLI100 |
DNA section
IS Length : 1269 bp
Ends
IR Length : 22/26
IRL : TGGATATATACTAATAAAGTAGACAGTTTTTTTAATGGTATACTGTTTAT
IRR : TGGATAGATGTCAATAAAGTAGACAGAAAAAAGGAATATTTTATGAAGAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| tgaacatcaacttctacagt | aagaagccttgacccgaatt | 0 | |
| tgaacatcaacttctacagt | accttcacaaacaacggaca | 0 |
DNA sequence
TGGATATATACTAATAAAGTAGACAGTTTTTTTAATGGTATACTGTTTATAAAGTAGACACTAAAACGAAAGGAACTACAGCTTATGTCTAATTACAAAA
AATATGATGAAGATTTTAAAAAGAGTCTAGTAAATTTATACAACGGAGGTAAAACACAAGCTGCTTTATGCAAAGAATATGGTGTCTCTCAAACCGCTCT
TTCTAGATGGATTAAACTTTATTCCGAAGTCCAAATGGAATCTGGCGAACTGCTAACTGTCAAACAAGTTCGCGAACTTCAAAAACAGAAAGCCTTGCTA
GAAGAAGAAAATTTAATCTTAAAAAAAGCGATTGCCATCTTCACGCCACACTCGAGCAACGATTAAAGGCTATTTCTCTGTTAGCTACCGATCATTCTAT
TGTAAGACTCTGCCAAGTATTGCGAGTGAACCGCAGTACCTATTACAAATTTTTATCCAAAATCCCTTCGGCTCGTTCCGTGGAAAACCAACAAATCAAA
CGGATTATTTTACAAATTTATACCGAGACAAAGTTTAGACTCGGCGCAGCCAAGATAAAAATAGTTTTACAGCGTGATTATGGTATTTCCATTAGTATCG
GAAGAGTGTATCGACTAATGAAAACCATGGATCTTCCTAAAATGTCTACCGTAAAACCAAGATTCCGTTATCAAAAACCGACGGTTTCTTCTACTTGTCC
AAATCTGTTGAAACAGCAATTTCATTCCAAGCAACCCAATCGTATTTGGGTCAGCGACATTTCGTATATTCCTGTAAAAAAAGGATTTGTTTATCTTTGT
GTAATTCTTGACATCTTCTCTCGAAAAGTGATTGCTTGGAATGTCTATGCCTCTATGACTGCAAAATTAGTTTGTGATACGGTAGAGCGAGCCGTTCATT
CTAGAAATCCTGTGCAGTCGGTTATTTTTCACTCTGATCGTGGTTCCCAATATTTGTCTCAAGAATTACGGCAGGTCCAAGAAAAATACAACCTTATCCC
TTCCTATTCTAAACTTGCTTACCCGTGGGATAATGCCGTCACAGAATCTTTCTTTAAATACATGAAGAAAGAAGAACTTAATCGTCGAAATTTTTCCTCT
TTGGAAGAAGTAAAATTAGCTTGTTTTGAGTATATTGAAGGATTCTATAATTCAAAAAGACCACATTCTGCAAACAACTTCTTATCACCTAATTTAAAAG
AACTTGCTTTTCTAAATGATTCTTCATAAAATATTCCTTTTTTCTGTCTACTTTATTGACATCTATCCA
AATATGATGAAGATTTTAAAAAGAGTCTAGTAAATTTATACAACGGAGGTAAAACACAAGCTGCTTTATGCAAAGAATATGGTGTCTCTCAAACCGCTCT
TTCTAGATGGATTAAACTTTATTCCGAAGTCCAAATGGAATCTGGCGAACTGCTAACTGTCAAACAAGTTCGCGAACTTCAAAAACAGAAAGCCTTGCTA
GAAGAAGAAAATTTAATCTTAAAAAAAGCGATTGCCATCTTCACGCCACACTCGAGCAACGATTAAAGGCTATTTCTCTGTTAGCTACCGATCATTCTAT
TGTAAGACTCTGCCAAGTATTGCGAGTGAACCGCAGTACCTATTACAAATTTTTATCCAAAATCCCTTCGGCTCGTTCCGTGGAAAACCAACAAATCAAA
CGGATTATTTTACAAATTTATACCGAGACAAAGTTTAGACTCGGCGCAGCCAAGATAAAAATAGTTTTACAGCGTGATTATGGTATTTCCATTAGTATCG
GAAGAGTGTATCGACTAATGAAAACCATGGATCTTCCTAAAATGTCTACCGTAAAACCAAGATTCCGTTATCAAAAACCGACGGTTTCTTCTACTTGTCC
AAATCTGTTGAAACAGCAATTTCATTCCAAGCAACCCAATCGTATTTGGGTCAGCGACATTTCGTATATTCCTGTAAAAAAAGGATTTGTTTATCTTTGT
GTAATTCTTGACATCTTCTCTCGAAAAGTGATTGCTTGGAATGTCTATGCCTCTATGACTGCAAAATTAGTTTGTGATACGGTAGAGCGAGCCGTTCATT
CTAGAAATCCTGTGCAGTCGGTTATTTTTCACTCTGATCGTGGTTCCCAATATTTGTCTCAAGAATTACGGCAGGTCCAAGAAAAATACAACCTTATCCC
TTCCTATTCTAAACTTGCTTACCCGTGGGATAATGCCGTCACAGAATCTTTCTTTAAATACATGAAGAAAGAAGAACTTAATCGTCGAAATTTTTCCTCT
TTGGAAGAAGTAAAATTAGCTTGTTTTGAGTATATTGAAGGATTCTATAATTCAAAAAGACCACATTCTGCAAACAACTTCTTATCACCTAATTTAAAAG
AACTTGCTTTTCTAAATGATTCTTCATAAAATATTCCTTTTTTCTGTCTACTTTATTGACATCTATCCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 282 bp | 93 aa | 85 | 366 | + | No |
Description : First part of the transposase
ORF sequence :
MSNYKKYDEDFKKSLVNLYNGGKTQAALCKEYGVSQTALSRWIKLYSEVQMESGELLTVKQVRELQKQKALLEEENLILKKAIAIFTPHSSND
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1014 bp | 337 aa | 216 | 1229 | + | No |
Description : Second part of the transposase
ORF sequence :
TLFRSPNGIWRTANCQTSSRTSKTESLARRRKFNLKKSDCHLHATLEQRLKAISLLATDHSIVRLCQVLRVNRSTYYKFLSKIPSARSVENQQIKRIILQ
IYTETKFRLGAAKIKIVLQRDYGISISIGRVYRLMKTMDLPKMSTVKPRFRYQKPTVSSTCPNLLKQQFHSKQPNRIWVSDISYIPVKKGFVYLCVILDI
FSRKVIAWNVYASMTAKLVCDTVERAVHSRNPVQSVIFHSDRGSQYLSQELRQVQEKYNLIPSYSKLAYPWDNAVTESFFKYMKKEELNRRNFSSLEEVK
LACFEYIEGFYNSKRPHSANNFLSPNLKELAFLNDSS
IYTETKFRLGAAKIKIVLQRDYGISISIGRVYRLMKTMDLPKMSTVKPRFRYQKPTVSSTCPNLLKQQFHSKQPNRIWVSDISYIPVKKGFVYLCVILDI
FSRKVIAWNVYASMTAKLVCDTVERAVHSRNPVQSVIFHSDRGSQYLSQELRQVQEKYNLIPSYSKLAYPWDNAVTESFFKYMKKEELNRRNFSSLEEVK
LACFEYIEGFYNSKRPHSANNFLSPNLKELAFLNDSS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1145 bp | 381 aa | 85 | 1229 | + | Yes |
Chemistry : DDE
ORF sequence :
MSNYKKYDEDFKKSLVNLYNGGKTQAALCKEYGVSQTALSRWIKLYSEVQMESGELLTVKQVRELQKQKALLEEENLILKKSDCHLHATLEQRLKAISLL
ATDHSIVRLCQVLRVNRSTYYKFLSKIPSARSVENQQIKRIILQIYTETKFRLGAAKIKIVLQRDYGISISIGRVYRLMKTMDLPKMSTVKPRFRYQKPT
VSSTCPNLLKQQFHSKQPNRIWVSDISYIPVKKGFVYLCVILDIFSRKVIAWNVYASMTAKLVCDTVERAVHSRNPVQSVIFHSDRGSQYLSQELRQVQE
KYNLIPSYSKLAYPWDNAVTESFFKYMKKEELNRRNFSSLEEVKLACFEYIEGFYNSKRPHSANNFLSPNLKELAFLNDSS
ATDHSIVRLCQVLRVNRSTYYKFLSKIPSARSVENQQIKRIILQIYTETKFRLGAAKIKIVLQRDYGISISIGRVYRLMKTMDLPKMSTVKPRFRYQKPT
VSSTCPNLLKQQFHSKQPNRIWVSDISYIPVKKGFVYLCVILDIFSRKVIAWNVYASMTAKLVCDTVERAVHSRNPVQSVIFHSDRGSQYLSQELRQVQE
KYNLIPSYSKLAYPWDNAVTESFFKYMKKEELNRRNFSSLEEVKLACFEYIEGFYNSKRPHSANNFLSPNLKELAFLNDSS
Blast result :
Comments
ISLgr1is 83% aa similar to ISLse3.
ISLgr1 was identified by in silico sequence analysis of L. grayi 19/09/S plasmid pLIS54.
The third ORF is the putative transposase reconstructed in silico by possible -1 frameshift.
ISLgr1 was identified by in silico sequence analysis of L. grayi 19/09/S plasmid pLIS54.
The third ORF is the putative transposase reconstructed in silico by possible -1 frameshift.
References
1] Chmielowska, C. and Bartosik, D. (2021) Direct submission.