ISAve5

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AP022290.1 ND Aeromonas veronii
Aeromonas caviae Aero21
Aeromonas veronii WP9-W18-ESBL-04
Aeromonas allosaccharophila FDAARGOS_933
Aeromonas media R50-22
Aeromonas media R25-3
Aeromonas hydrophila HX-3
Aeromonas caviae Aero21 plasmid p1
Aeromonas caviae KAM329
Aeromonas hydrophila 2359
DNA section
IS Length : 2627 bp

Ends


IR Length : 39/50

IRL : TGCGTATTCCCACGCATGGCGATCGCTCAATAACATTCATGCCGATCACT
IRR : TGGCTAATAACATCATGTCGATCAGCCAATAACATCATGCCGATCACCAT

Insertion site


Left flankDirect repeatRight flankDR Length
CTTAGAACAATGGTCATGACGAGGCGAGCCTGTAAGC6
GCGGAGCCGCATTGCGATTTACCGGAAAACGGGTCT6

DNA sequence

TGCGTATTCCCACGCATGGCGATCGCTCAATAACATTCATGCCGATCACTCAATAACATCCATGCCGATCGCCCAATAACATCCCAAGCCGATCACTTTT
TTCCTCTATGGGAAACCATGATCGGCATGAATGTTATTGATGACTTCTTCTCCCCGCTTCGTTAAACCCGCACTTTCCAACTGGACCCAAAAGAGGAAAG
TGCATGTCTGCGAGGCCTATCACCGTGAGAAAACTCAAAGAAATCCTTCGTCTCAAATATCAGGCTCAACTGGCGCATCGTCAGATCGCCCTCAGCCTGC
ATATCTCATCCAGCACCGTCTCCTATTACCTCAATCGCGCTGCTCAGTTGGATATCACTACCTGGCCGCTCTGTGAGCGCTGGGATGATGTCACCCTGGA
GCGTGCCTTCCTCAGCACCCGCGCGGTGTCCAAAACCCGCCACAAACCGCTCCCTGACTGGGCCGTGCTCCATCAGGAGCTCAAACAGCCCGGTCAAAAA
GGGGTAACGCTGGAGCTACTGTGGCAGGAGTATGCCGAGCGCAATAACGGTGCCCACTACAGCTACAACCATTTCTGTCGGCTCTATAAGGAGTGGGCAT
CGGTGCTGCAACCGTCGATGCGCCAGACCCATATCGCGGGCGAAAAACTCTTTGTCGATTACTGTGGCCAGACCATGCCCATCGTGGATCCTCATACCGG
TGAGATCCTCTATCGGGCCCAGGTCTTCGTCGCGGTGCTCGGGGCCTCCAACTACACCTTTGCTGAAGCCACCCGCTCCCAACAGCTTGAGGATTGGGTG
ATGAGCCACAAGCGGGCCTTTGAGTTTTTCGGCGGCGTCCCGCAACTGGTGGTGCCTGATTGCTTGAAGAGTGCGGTCAGCGTGGCACGCAATACTGACC
CGGATCTCAATCCCACCTACCAGATGCTGGCCGCTCACTTCGGCACAGCGATCATGCCCGCCAGGCCGCGCAAGCCGAAAGATAAATCCAAGGCCGAGGT
GGGGGTACAGATCGTCACCCGCTGGATCCTGGCGGTACTGCGCCATGAGACCTTCCATAGCCTGGCTCAGCTTAACCAACGGATATCCGGGTTACTGACC
CGGCTCAATGAGAAACCCTTCCAGAAGCTGCCCGGTTGCCGCGCATCCATGTTTGCCGAGCTCGATGCGCCAGCACTCAAGCCCTTGCCAGCGTACCCCT
ACCAGTACACCAAAGTGGTGTCGGTGCTGGTCGGCAAGGATTACCACGTCGACCTGGAGAAGCACTATTACTCGGTCCCTTATGCCTTGCAGGGCAAGCG
GCTGGAAGCCCATCTGGCGGAGCGTACGGTCACCCTGTATCACGCGGGTAAGGTCGTCGCTCAGCACCCACGCAGTCATCGCAAAGGCCAGCACAGCAGT
CTGCCCGCCCATATGCCAGCGCATCACCAGGCACTGCTGGAGTGCTCACCTGAACGCCTACTGAGCTGGGCCGCGTCCATCGGGCCCTATACCACGCATT
GGGTGGATAGTTTTATCCGCCAGGCAGCGCATCCGGCCCAAGCCGTGCGACCTTGTCAGGCCCTGCTGGGACAGAGCAAAAAGTATGGCAAGGCGCGGCT
GGAGGCCGCCTGTCTACGTGGCTATCTGACCGGCGCCAACCGGCTGCACCACATCCGCAACATGCTCAAACACGGGCTGGAACAACAACCAGTTGCCACC
TCACGCCAGGATCCGTTGCAAGACATCCATCATGACAATGTCCACGGCGAACACTACTTCCACTAATCCATCACTCAACAAGGAAAAGAGCATGCAATTA
CATCTCGATGAACAACTGAGCACCCTGCGTCTGGGCGGTGTGAAACAGGCGCTGAGTCAGCAACAGACACAGAGCGCGTTGTATCAGGACATGAGCTTTG
AAGAGCGGCTGCAACTGCTGCTGAGTCATGAGCTGGTGCAACGGGAGCAGCGCAAAGTTAACCGGTTGGAGAAGCAAGCGCGGTTTCGATTGTCCGGTCA
AATTGAGCAGCTGGACTACCGTGCCAGCCGGGGAATAAGCAAAGCGCAGATCCGCCAGCTACTGGAAGGGCAGTGGCTGGCTCATCAGCAAAACCTGCTG
CTGACGGGGGCGGCAGGCTGTGGGAAGAGTTACCTGGCCTGTGCGTTGGGCCGCTATTTTATCCGGCAAGGCGTAGCGGTGCGCTACTACCGGCTGAAAA
CCCTGCTGGAAGAGATGAGACTGGCGCAGGCGGATGGCAGTTATCCGAAGCTGCTGGAGCAGGTGAATAATGTGGGCTTGCTGATCCTGGATGACTGGGG
CATGGAGATGCTGGATGCGGCCGAGCGCAGTAATTTGCTGGAGATCATCGATACCCGTTACGGGAGGGTCTCGACAGTGGTTGCGAGCCAACTGCCGGTA
GAGAAATGGTATGGGATGATCGGAGAGGCCACATTCGCAGAAGCGATCCTGGACCGGCTGATCCACAGAGCGGTACGAGTAGGTTTGACAGGTGAGTCGA
TGCGAAAACAGGGCATCAACTTGACCGATGCCGATCAGGCAGAGTAAAAGAAAGACGCAGTTCCTGCGGGTAGAAAAATGGTGATCGGCATGATGTTATT
GGCTGATCGACATGATGTTATTAGCCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1563 bp520 aa2041766+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSARPITVRKLKEILRLKYQAQLAHRQIALSLHISSSTVSYYLNRAAQLDITTWPLCERWDDVTLERAFLSTRAVSKTRHKPLPDWAVLHQELKQPGQKG
VTLELLWQEYAERNNGAHYSYNHFCRLYKEWASVLQPSMRQTHIAGEKLFVDYCGQTMPIVDPHTGEILYRAQVFVAVLGASNYTFAEATRSQQLEDWVM
SHKRAFEFFGGVPQLVVPDCLKSAVSVARNTDPDLNPTYQMLAAHFGTAIMPARPRKPKDKSKAEVGVQIVTRWILAVLRHETFHSLAHLNQRISGLLTR
LNEKPFQKLPGCRASMFAELDAPALKPLPAYPYQYTKVVSVLVGKDYHVDLEKHYYSVPYALQGKRLEAHLAERTVTLYHAGKVVAQHPRSHRKGQHSSL
PAHMPAHHQALLECSPERLLSWAASIGPYTTHWVDSFIRQAAHPAQAVRPCQALLGQSKKYGKARLEAACLRGYLTGANRLHHIRNMLKHGLEQQPVATS
RQDPLQDIHHDNVHGEHYFH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa17922547+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MQLHLDEQLSTLRLGGVKQALSQQQTQSALYQDMSFEERLQLLLSHELVQREQRKVNRLEKQARFRLSGQIEQLDYRASRGISKAQIRQLLEGQWLAHQQ
NLLLTGAAGCGKSYLACALGRYFIRQGVAVRYYRLKTLLEEMRLAQADGSYPKLLEQVNNVGLLILDDWGMEMLDAAERSNLLEIIDTRYGRVSTVVASQ
LPVEKWYGMIGEATFAEAILDRLIHRAVRVGLTGESMRKQGINLTDADQAE

 

Blast result :
Comments
ISAve5 is 94% aa (transposase) similar to ISAs28.
References
1] Hong Du (2021) Direct submission.
2] Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M. (2019) Direct GenBank submission.