ISAdh1
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP032839 | ND | Aeromonas dhakensis | Aeromonas dhakensis 1706-28330 Aeromonas veronii FC951 |
DNA section
IS Length : 2450 bp
Ends
IR Length : 17/22
IRL : GTAAGCGTCCGGCCACTACCGTCTGGTGAATGGAGGCAGTTGTGGACAAA
IRR : GTAAGCGTCCAGCGAAGGCCGTATTGACAGGGGAGACAGGTTATTCAGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAACGGAACAGCG | CGCTATCT | CCTTCTACGGTG | 8 |
DNA sequence
GTAAGCGTCCGGCCACTACCGTCTGGTGAATGGAGGCAGTTGTGGACAAAGTAGTTCCCACTAAAAAGTAGTGGGAACCAAAGATGGTGTACACCAATAG
TCGTACCAATGGCAGCCGTAAAGGCAGAGCCAATTACTCACGAGAATTCAAACAGCGACTTGTCGAGGCCGCCAACCAACCCGGCGTCTCCGTCTCAAAA
CTCGCACAGGAGCACGGGATCAATGCAAACCTCTTGTTCAAATGGCGACGTGATGCCAAAGCCGAAGCCCCTGCGTTATATCCCATCGAACTGGTGTTGC
CCCCTTCATCCACGGAGATCTTCTTGCCTCCGACCCCGCAGCCCGAAATGCTCACGCCAGAGACGGCCTCTGAACCGACACCATCCGGCACCATCGAGAT
ACGACTGGGGCGTGCAATGGTCGTCATCGATGGCACCGTCGATACCAATGTATTACGAATGATCCTGGAGACCCTGCGCGTATGATCAATCTTCCTACTG
GCACCCGGATCTGGATCGTGGCCGGTATCACAGACATGAGAAACGGCTTCAATGGGTTAGCCTCGAAAGTGCAAATGACGTTGAAGGATGATCCCTTGTC
AGGGCATGTCTTTATCTTCAGGGGCCGTAACGGGAGTCAAGTTAAGCTCCTCTGGTCTACCGGCGATGGTCTGTGTCTGCTGACCAAAAGGCTGGAGCAG
GGTCGGTTTGCCTGGCCATCGGCTCGAAACGGCAAAGTCTTTTTGACGCCTGCACAACTGGCCATGCTACTGGAGGGAATAGACTGGCGCCATCCAAAGC
GCCTGGCTAACTCATTGACCATGTTGTAAAAAGTGATCCCCCATGTAAGATGCATGGGTATGGAAAAATTACTAGCCGACGAAATCCTGCGACTGAAACG
ACTCCTGGCTGAACAGGAGTCACTCAATCGTGTTTTGCATGCAAGGTTGGAAGAGCGGGAGCGCGAAATTGAGCGCCTGCTTGCGCAACTCGACAAGCTG
CGGCGTATGTATTTTGGCAGCCAGTCCGAGAAACTCGCTCGTCGTATCGCTCAGACCGAAGCGCAACTGCAAATACTCCAGCAGGAGAGTGATACCTTCA
CCGGTCGGGTCGATGATCCAGCGGTACCGCGGCCACTTCGCCAGACTCGCATTCGCAAACCCTTCCCTCAAGCGCTTCCCCGCGATGAGCATCGCCTGTG
TCCAACAGAGGCTGTCTGCCCGGAATGTGGTGGAGCGCTGGCATACCTTGGAGAAGATACTGCTGAGCAGTTAGAGCTGATACGCAGTGCCTTCCGGGTC
ATCCGCACGGTTCGGGAGAAACACACCTGCGGGCAATGCGATTTCATCATACAAGCGCCAGCCCCATCACGTCCTATCGAGCGGGGTATCGCCGGGCCAG
GCTTACTGGCTCGGGTACTCACCTCCAAGTATGCAGAGCACACCCCGCTGTATCGCCAGTCGGAAATCTATGCCCGTCAGGGCGTGGAGCTGAGCCGCTC
TGTGCTCTCTGGCTGGGTAGATGCGTGTTGCCGATTACTGTCACCGCTGGAGGAAGCGCTGCGGCAGTATGTGCTGACCGATGGCAAACTACACGCAGAC
GATACCCCGGTGCCGGTACTGCTCCCTGGCAATAAAAAGACAAAAACAGGACGTCTGTGGACGTATGTACGCGATGATCGCAACGCAGGCTCCGCACTGC
CGCCAGCCGTCTGGTTCGCTTACTCGCCTGATAGAAAGGGAGTCCATCCGCAAAGCCATCTTGCCGATTTCAGCGGCGTCCTACAGGCAGACGCATATGC
AGGATTTAATGAGCTATATCGTGAAGACCGAGCTGCCGGCACGATAAAGGAGGCCGCTTGCTGGGCTCATGCCAGGCGAAAGATCCATGATGTGCATATC
CGAACGCCATCGGATAACACAGACGAAGCCCTCAAACGAATTGGTCAGCTTTACAGCCTTGAAGCAGAACTGCGCGGCATGTCAGCAGAACACCGGCTGG
CAGAACGAGAACGGCGGGCAAAGCCCTTACTGAAAGAGCTGGAGATCTGGTTACGGGATAGGATGCAGTCGCTCTCGCGTCACTCCGAGTTGGCTAAGGC
CTTCGCCTATGCCTTGAATCAGTGGTCAGCACTGACGTACTACACCGAAAACGGCTGGATAGAAGTGGACAACAACATAGCGGAGAATGCGCTACGGCTG
GTGAGCTTGGGGCGAAAGAACTTCCTGTTTTTTGGCTCTGACCATGGTGGTGAACGGGGAGCCTTGTTGTACAGCTTGCTCGGCACCTGCCGGTTGAACG
GGGTAGATCCGGAGCATTACCTGCGGCATGTGCTGAATGTGATCGCAGACTGGCCGGTGAACCGGGTCAGCGAACTGCTGCCCTGGCGTGTTGCTCTACC
CGCTGAATAACCTGTCTCCCCTGTCAATACGGCCTTCGCTGGACGCTTAC
TCGTACCAATGGCAGCCGTAAAGGCAGAGCCAATTACTCACGAGAATTCAAACAGCGACTTGTCGAGGCCGCCAACCAACCCGGCGTCTCCGTCTCAAAA
CTCGCACAGGAGCACGGGATCAATGCAAACCTCTTGTTCAAATGGCGACGTGATGCCAAAGCCGAAGCCCCTGCGTTATATCCCATCGAACTGGTGTTGC
CCCCTTCATCCACGGAGATCTTCTTGCCTCCGACCCCGCAGCCCGAAATGCTCACGCCAGAGACGGCCTCTGAACCGACACCATCCGGCACCATCGAGAT
ACGACTGGGGCGTGCAATGGTCGTCATCGATGGCACCGTCGATACCAATGTATTACGAATGATCCTGGAGACCCTGCGCGTATGATCAATCTTCCTACTG
GCACCCGGATCTGGATCGTGGCCGGTATCACAGACATGAGAAACGGCTTCAATGGGTTAGCCTCGAAAGTGCAAATGACGTTGAAGGATGATCCCTTGTC
AGGGCATGTCTTTATCTTCAGGGGCCGTAACGGGAGTCAAGTTAAGCTCCTCTGGTCTACCGGCGATGGTCTGTGTCTGCTGACCAAAAGGCTGGAGCAG
GGTCGGTTTGCCTGGCCATCGGCTCGAAACGGCAAAGTCTTTTTGACGCCTGCACAACTGGCCATGCTACTGGAGGGAATAGACTGGCGCCATCCAAAGC
GCCTGGCTAACTCATTGACCATGTTGTAAAAAGTGATCCCCCATGTAAGATGCATGGGTATGGAAAAATTACTAGCCGACGAAATCCTGCGACTGAAACG
ACTCCTGGCTGAACAGGAGTCACTCAATCGTGTTTTGCATGCAAGGTTGGAAGAGCGGGAGCGCGAAATTGAGCGCCTGCTTGCGCAACTCGACAAGCTG
CGGCGTATGTATTTTGGCAGCCAGTCCGAGAAACTCGCTCGTCGTATCGCTCAGACCGAAGCGCAACTGCAAATACTCCAGCAGGAGAGTGATACCTTCA
CCGGTCGGGTCGATGATCCAGCGGTACCGCGGCCACTTCGCCAGACTCGCATTCGCAAACCCTTCCCTCAAGCGCTTCCCCGCGATGAGCATCGCCTGTG
TCCAACAGAGGCTGTCTGCCCGGAATGTGGTGGAGCGCTGGCATACCTTGGAGAAGATACTGCTGAGCAGTTAGAGCTGATACGCAGTGCCTTCCGGGTC
ATCCGCACGGTTCGGGAGAAACACACCTGCGGGCAATGCGATTTCATCATACAAGCGCCAGCCCCATCACGTCCTATCGAGCGGGGTATCGCCGGGCCAG
GCTTACTGGCTCGGGTACTCACCTCCAAGTATGCAGAGCACACCCCGCTGTATCGCCAGTCGGAAATCTATGCCCGTCAGGGCGTGGAGCTGAGCCGCTC
TGTGCTCTCTGGCTGGGTAGATGCGTGTTGCCGATTACTGTCACCGCTGGAGGAAGCGCTGCGGCAGTATGTGCTGACCGATGGCAAACTACACGCAGAC
GATACCCCGGTGCCGGTACTGCTCCCTGGCAATAAAAAGACAAAAACAGGACGTCTGTGGACGTATGTACGCGATGATCGCAACGCAGGCTCCGCACTGC
CGCCAGCCGTCTGGTTCGCTTACTCGCCTGATAGAAAGGGAGTCCATCCGCAAAGCCATCTTGCCGATTTCAGCGGCGTCCTACAGGCAGACGCATATGC
AGGATTTAATGAGCTATATCGTGAAGACCGAGCTGCCGGCACGATAAAGGAGGCCGCTTGCTGGGCTCATGCCAGGCGAAAGATCCATGATGTGCATATC
CGAACGCCATCGGATAACACAGACGAAGCCCTCAAACGAATTGGTCAGCTTTACAGCCTTGAAGCAGAACTGCGCGGCATGTCAGCAGAACACCGGCTGG
CAGAACGAGAACGGCGGGCAAAGCCCTTACTGAAAGAGCTGGAGATCTGGTTACGGGATAGGATGCAGTCGCTCTCGCGTCACTCCGAGTTGGCTAAGGC
CTTCGCCTATGCCTTGAATCAGTGGTCAGCACTGACGTACTACACCGAAAACGGCTGGATAGAAGTGGACAACAACATAGCGGAGAATGCGCTACGGCTG
GTGAGCTTGGGGCGAAAGAACTTCCTGTTTTTTGGCTCTGACCATGGTGGTGAACGGGGAGCCTTGTTGTACAGCTTGCTCGGCACCTGCCGGTTGAACG
GGGTAGATCCGGAGCATTACCTGCGGCATGTGCTGAATGTGATCGCAGACTGGCCGGTGAACCGGGTCAGCGAACTGCTGCCCTGGCGTGTTGCTCTACC
CGCTGAATAACCTGTCTCCCCTGTCAATACGGCCTTCGCTGGACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 402 bp | 133 aa | 84 | 485 | + | No |
AG : IS66 TnpA
ORF sequence :
MVYTNSRTNGSRKGRANYSREFKQRLVEAANQPGVSVSKLAQEHGINANLLFKWRRDAKAEAPALYPIELVLPPSSTEIFLPPTPQPEMLTPETASEPTP
SGTIEIRLGRAMVVIDGTVDTNVLRMILETLRV
SGTIEIRLGRAMVVIDGTVDTNVLRMILETLRV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 348 bp | 115 aa | 482 | 829 | + | No |
AG : IS66 TnpB
ORF sequence :
MINLPTGTRIWIVAGITDMRNGFNGLASKVQMTLKDDPLSGHVFIFRGRNGSQVKLLWSTGDGLCLLTKRLEQGRFAWPSARNGKVFLTPAQLAMLLEGI
DWRHPKRLANSLTML
DWRHPKRLANSLTML
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1551 bp | 516 aa | 860 | 2410 | + | No |
Chemistry : DDE
ORF sequence :
MEKLLADEILRLKRLLAEQESLNRVLHARLEEREREIERLLAQLDKLRRMYFGSQSEKLARRIAQTEAQLQILQQESDTFTGRVDDPAVPRPLRQTRIRK
PFPQALPRDEHRLCPTEAVCPECGGALAYLGEDTAEQLELIRSAFRVIRTVREKHTCGQCDFIIQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQ
SEIYARQGVELSRSVLSGWVDACCRLLSPLEEALRQYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNAGSALPPAVWFAYSPDRKGVHPQSH
LADFSGVLQADAYAGFNELYREDRAAGTIKEAACWAHARRKIHDVHIRTPSDNTDEALKRIGQLYSLEAELRGMSAEHRLAERERRAKPLLKELEIWLRD
RMQSLSRHSELAKAFAYALNQWSALTYYTENGWIEVDNNIAENALRLVSLGRKNFLFFGSDHGGERGALLYSLLGTCRLNGVDPEHYLRHVLNVIADWPV
NRVSELLPWRVALPAE
PFPQALPRDEHRLCPTEAVCPECGGALAYLGEDTAEQLELIRSAFRVIRTVREKHTCGQCDFIIQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQ
SEIYARQGVELSRSVLSGWVDACCRLLSPLEEALRQYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNAGSALPPAVWFAYSPDRKGVHPQSH
LADFSGVLQADAYAGFNELYREDRAAGTIKEAACWAHARRKIHDVHIRTPSDNTDEALKRIGQLYSLEAELRGMSAEHRLAERERRAKPLLKELEIWLRD
RMQSLSRHSELAKAFAYALNQWSALTYYTENGWIEVDNNIAENALRLVSLGRKNFLFFGSDHGGERGALLYSLLGTCRLNGVDPEHYLRHVLNVIADWPV
NRVSELLPWRVALPAE
Blast result :
Comments
ISAdh1 is 90% aa (transposase)similar to ISSgsp1.
The copy in Aeromonas dhakensis strain 1706-28330 (CP054854) had a mutation in the transposase (premature STOP codon) and was replaced by the copy found in Aeromonas veronii strain FC951 (CP032839).
The copy in Aeromonas dhakensis strain 1706-28330 (CP054854) had a mutation in the transposase (premature STOP codon) and was replaced by the copy found in Aeromonas veronii strain FC951 (CP032839).
References
1] Dongsheng Zhou (2021) Direct submission.
2] Veeraraghavan,B., Devanga Ragupathi,N.K., Muthuirulandi ,D.P., Vasudevan,K. and Anandan,S. (2018) Direct GenBank submission.
3] Zhou,D. (2020) Direct GenBank submission.
2] Veeraraghavan,B., Devanga Ragupathi,N.K., Muthuirulandi ,D.P., Vasudevan,K. and Anandan,S. (2018) Direct GenBank submission.
3] Zhou,D. (2020) Direct GenBank submission.