ISAdh1

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP032839 ND Aeromonas dhakensis
Aeromonas dhakensis 1706-28330
Aeromonas veronii FC951
DNA section
IS Length : 2450 bp

Ends


IR Length : 17/22

IRL : GTAAGCGTCCGGCCACTACCGTCTGGTGAATGGAGGCAGTTGTGGACAAA
IRR : GTAAGCGTCCAGCGAAGGCCGTATTGACAGGGGAGACAGGTTATTCAGCG

Insertion site


Left flankDirect repeatRight flankDR Length
CAACGGAACAGCGCGCTATCTCCTTCTACGGTG8

DNA sequence

GTAAGCGTCCGGCCACTACCGTCTGGTGAATGGAGGCAGTTGTGGACAAAGTAGTTCCCACTAAAAAGTAGTGGGAACCAAAGATGGTGTACACCAATAG
TCGTACCAATGGCAGCCGTAAAGGCAGAGCCAATTACTCACGAGAATTCAAACAGCGACTTGTCGAGGCCGCCAACCAACCCGGCGTCTCCGTCTCAAAA
CTCGCACAGGAGCACGGGATCAATGCAAACCTCTTGTTCAAATGGCGACGTGATGCCAAAGCCGAAGCCCCTGCGTTATATCCCATCGAACTGGTGTTGC
CCCCTTCATCCACGGAGATCTTCTTGCCTCCGACCCCGCAGCCCGAAATGCTCACGCCAGAGACGGCCTCTGAACCGACACCATCCGGCACCATCGAGAT
ACGACTGGGGCGTGCAATGGTCGTCATCGATGGCACCGTCGATACCAATGTATTACGAATGATCCTGGAGACCCTGCGCGTATGATCAATCTTCCTACTG
GCACCCGGATCTGGATCGTGGCCGGTATCACAGACATGAGAAACGGCTTCAATGGGTTAGCCTCGAAAGTGCAAATGACGTTGAAGGATGATCCCTTGTC
AGGGCATGTCTTTATCTTCAGGGGCCGTAACGGGAGTCAAGTTAAGCTCCTCTGGTCTACCGGCGATGGTCTGTGTCTGCTGACCAAAAGGCTGGAGCAG
GGTCGGTTTGCCTGGCCATCGGCTCGAAACGGCAAAGTCTTTTTGACGCCTGCACAACTGGCCATGCTACTGGAGGGAATAGACTGGCGCCATCCAAAGC
GCCTGGCTAACTCATTGACCATGTTGTAAAAAGTGATCCCCCATGTAAGATGCATGGGTATGGAAAAATTACTAGCCGACGAAATCCTGCGACTGAAACG
ACTCCTGGCTGAACAGGAGTCACTCAATCGTGTTTTGCATGCAAGGTTGGAAGAGCGGGAGCGCGAAATTGAGCGCCTGCTTGCGCAACTCGACAAGCTG
CGGCGTATGTATTTTGGCAGCCAGTCCGAGAAACTCGCTCGTCGTATCGCTCAGACCGAAGCGCAACTGCAAATACTCCAGCAGGAGAGTGATACCTTCA
CCGGTCGGGTCGATGATCCAGCGGTACCGCGGCCACTTCGCCAGACTCGCATTCGCAAACCCTTCCCTCAAGCGCTTCCCCGCGATGAGCATCGCCTGTG
TCCAACAGAGGCTGTCTGCCCGGAATGTGGTGGAGCGCTGGCATACCTTGGAGAAGATACTGCTGAGCAGTTAGAGCTGATACGCAGTGCCTTCCGGGTC
ATCCGCACGGTTCGGGAGAAACACACCTGCGGGCAATGCGATTTCATCATACAAGCGCCAGCCCCATCACGTCCTATCGAGCGGGGTATCGCCGGGCCAG
GCTTACTGGCTCGGGTACTCACCTCCAAGTATGCAGAGCACACCCCGCTGTATCGCCAGTCGGAAATCTATGCCCGTCAGGGCGTGGAGCTGAGCCGCTC
TGTGCTCTCTGGCTGGGTAGATGCGTGTTGCCGATTACTGTCACCGCTGGAGGAAGCGCTGCGGCAGTATGTGCTGACCGATGGCAAACTACACGCAGAC
GATACCCCGGTGCCGGTACTGCTCCCTGGCAATAAAAAGACAAAAACAGGACGTCTGTGGACGTATGTACGCGATGATCGCAACGCAGGCTCCGCACTGC
CGCCAGCCGTCTGGTTCGCTTACTCGCCTGATAGAAAGGGAGTCCATCCGCAAAGCCATCTTGCCGATTTCAGCGGCGTCCTACAGGCAGACGCATATGC
AGGATTTAATGAGCTATATCGTGAAGACCGAGCTGCCGGCACGATAAAGGAGGCCGCTTGCTGGGCTCATGCCAGGCGAAAGATCCATGATGTGCATATC
CGAACGCCATCGGATAACACAGACGAAGCCCTCAAACGAATTGGTCAGCTTTACAGCCTTGAAGCAGAACTGCGCGGCATGTCAGCAGAACACCGGCTGG
CAGAACGAGAACGGCGGGCAAAGCCCTTACTGAAAGAGCTGGAGATCTGGTTACGGGATAGGATGCAGTCGCTCTCGCGTCACTCCGAGTTGGCTAAGGC
CTTCGCCTATGCCTTGAATCAGTGGTCAGCACTGACGTACTACACCGAAAACGGCTGGATAGAAGTGGACAACAACATAGCGGAGAATGCGCTACGGCTG
GTGAGCTTGGGGCGAAAGAACTTCCTGTTTTTTGGCTCTGACCATGGTGGTGAACGGGGAGCCTTGTTGTACAGCTTGCTCGGCACCTGCCGGTTGAACG
GGGTAGATCCGGAGCATTACCTGCGGCATGTGCTGAATGTGATCGCAGACTGGCCGGTGAACCGGGTCAGCGAACTGCTGCCCTGGCGTGTTGCTCTACC
CGCTGAATAACCTGTCTCCCCTGTCAATACGGCCTTCGCTGGACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
402 bp133 aa84485+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MVYTNSRTNGSRKGRANYSREFKQRLVEAANQPGVSVSKLAQEHGINANLLFKWRRDAKAEAPALYPIELVLPPSSTEIFLPPTPQPEMLTPETASEPTP
SGTIEIRLGRAMVVIDGTVDTNVLRMILETLRV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp115 aa482829+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MINLPTGTRIWIVAGITDMRNGFNGLASKVQMTLKDDPLSGHVFIFRGRNGSQVKLLWSTGDGLCLLTKRLEQGRFAWPSARNGKVFLTPAQLAMLLEGI
DWRHPKRLANSLTML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1551 bp516 aa8602410+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEKLLADEILRLKRLLAEQESLNRVLHARLEEREREIERLLAQLDKLRRMYFGSQSEKLARRIAQTEAQLQILQQESDTFTGRVDDPAVPRPLRQTRIRK
PFPQALPRDEHRLCPTEAVCPECGGALAYLGEDTAEQLELIRSAFRVIRTVREKHTCGQCDFIIQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQ
SEIYARQGVELSRSVLSGWVDACCRLLSPLEEALRQYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNAGSALPPAVWFAYSPDRKGVHPQSH
LADFSGVLQADAYAGFNELYREDRAAGTIKEAACWAHARRKIHDVHIRTPSDNTDEALKRIGQLYSLEAELRGMSAEHRLAERERRAKPLLKELEIWLRD
RMQSLSRHSELAKAFAYALNQWSALTYYTENGWIEVDNNIAENALRLVSLGRKNFLFFGSDHGGERGALLYSLLGTCRLNGVDPEHYLRHVLNVIADWPV
NRVSELLPWRVALPAE

 

Blast result :
Comments
ISAdh1 is 90% aa (transposase)similar to ISSgsp1.
The copy in Aeromonas dhakensis strain 1706-28330 (CP054854) had a mutation in the transposase (premature STOP codon) and was replaced by the copy found in Aeromonas veronii strain FC951 (CP032839).
References
1] Dongsheng Zhou (2021) Direct submission.
2] Veeraraghavan,B., Devanga Ragupathi,N.K., Muthuirulandi ,D.P., Vasudevan,K. and Anandan,S. (2018) Direct GenBank submission.
3] Zhou,D. (2020) Direct GenBank submission.