ISHati1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP039374 | ND | Halomonas titanicae | Halomonas titanicae ANRCS81 Halomonas sp. PA16-9 |
DNA section
IS Length : 1270 bp
Ends
IR Length : 24/28
IRL : TGGACTTCCCCCAGTTCAGTGGACACGCATCGATAACTCCGCAGGAGGAG
IRR : TGGACTTCCCCCAATAAAATGGACACGCCCCATCAGTTGATTCCTTCGAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAACGGACTC | ttttattggG | 0 | |
| CCCTTCAAGAGGG | GCA | GCCAATGCGTGGT | 3 |
DNA sequence
TGGACTTCCCCCAGTTCAGTGGACACGCATCGATAACTCCGCAGGAGGAGAAATACGATGCCGGAGATGATCACGGGGAAGAAAACTCAGCAGTACAGCA
CTGAGTTCAAGGTCAAAGCGGTGGAGTGGAGTCACCAGGCCCACCGGAGTGTGAAAAGCGTCGCCGAAGCGCTGGATATTCACCCTTTCATGCTGTCACG
CTGGCGTAAGGAATACCGAGAGGGAAAGTTCGCCATGAAACGGGTAAAGAAAGCGCCAGCAGACGCCAAGAAGAAGATCCAGGAACAGGATGAGGTTGCC
CGCCTGAAACGCCGGATATCGGAGCTTCAGGAGGAAAACGACATTCTAAAAAAGTTTCAACGTTTTCAGGCCGAGGAACGACGGAAGCGTTCCGGTTCAT
CTGGAAACACCGACGAGACTACAGTGTAAAAGCGCTGTGCAGACATCTGAAGGTGTCGCGGTCTGGATACTATGCCTGGGCCAACCGGGAGCCCAGCAAG
AGAGCCACTGACAAGGCAGACTTGTTGTTGAAGATCAGGAAGATCTACGACAACAGTAAAGGCCGTTATGGTAGTCCTCGCGTTTACCAGACGCTGCGCC
GAGAAGGCCTGGTGGTCGGAGAGAATCGGGTCGCGAAAATTATGCGGGAGTGGGGCATGAAGGCCCGCTCTGCTCGTGTGTACCGCCGATTGAGGAAGCT
CAGGAATGATCTGAAAGCCCTGCCAAATTATCGACTTGAGGCAGCGAGACCGACTGCGGAAAACCAGCAGTGGAGCAGTGACGTCACGTATATCAAGCTT
GGCAAGAAGTACGTGTTCCTGGCGGTAGTGTTGGACCTGTACTCTCGCCGCATTATCAGTTGGCGCTTGGGGGCGAGTCTGAGAGCAAACTTTGCCAGAG
CAACGTTGCGGGAGGCCTTCACAAGCCGTAAGCCGGATTCTGGTCTGTTGTTCCATACGGATCGCGGCATCGAATACCGAGCTCATAAGACACAAGCGCT
GCTGAAGCAGTACCAAGTAAAACACAGCATGAACCGACCTGGTCAGTGTGCCGACAATGCCGAGGTTGAATCGTTCTTCAAGACCCTCAAGGGAGAATTG
CTGCACGCCACCAGCTTCGTGACGATGCGCCAATTACGGAAACATATAAAAGCCTATATTGAGGGTTTTTATAATACCCAACGGCTGCACAGTGGCCTTG
GTTACCGGACTCCGCTAGAGTTCGAAGGAATCAACTGATGGGGCGTGTCCATTTTATTGGGGGAAGTCCA
CTGAGTTCAAGGTCAAAGCGGTGGAGTGGAGTCACCAGGCCCACCGGAGTGTGAAAAGCGTCGCCGAAGCGCTGGATATTCACCCTTTCATGCTGTCACG
CTGGCGTAAGGAATACCGAGAGGGAAAGTTCGCCATGAAACGGGTAAAGAAAGCGCCAGCAGACGCCAAGAAGAAGATCCAGGAACAGGATGAGGTTGCC
CGCCTGAAACGCCGGATATCGGAGCTTCAGGAGGAAAACGACATTCTAAAAAAGTTTCAACGTTTTCAGGCCGAGGAACGACGGAAGCGTTCCGGTTCAT
CTGGAAACACCGACGAGACTACAGTGTAAAAGCGCTGTGCAGACATCTGAAGGTGTCGCGGTCTGGATACTATGCCTGGGCCAACCGGGAGCCCAGCAAG
AGAGCCACTGACAAGGCAGACTTGTTGTTGAAGATCAGGAAGATCTACGACAACAGTAAAGGCCGTTATGGTAGTCCTCGCGTTTACCAGACGCTGCGCC
GAGAAGGCCTGGTGGTCGGAGAGAATCGGGTCGCGAAAATTATGCGGGAGTGGGGCATGAAGGCCCGCTCTGCTCGTGTGTACCGCCGATTGAGGAAGCT
CAGGAATGATCTGAAAGCCCTGCCAAATTATCGACTTGAGGCAGCGAGACCGACTGCGGAAAACCAGCAGTGGAGCAGTGACGTCACGTATATCAAGCTT
GGCAAGAAGTACGTGTTCCTGGCGGTAGTGTTGGACCTGTACTCTCGCCGCATTATCAGTTGGCGCTTGGGGGCGAGTCTGAGAGCAAACTTTGCCAGAG
CAACGTTGCGGGAGGCCTTCACAAGCCGTAAGCCGGATTCTGGTCTGTTGTTCCATACGGATCGCGGCATCGAATACCGAGCTCATAAGACACAAGCGCT
GCTGAAGCAGTACCAAGTAAAACACAGCATGAACCGACCTGGTCAGTGTGCCGACAATGCCGAGGTTGAATCGTTCTTCAAGACCCTCAAGGGAGAATTG
CTGCACGCCACCAGCTTCGTGACGATGCGCCAATTACGGAAACATATAAAAGCCTATATTGAGGGTTTTTATAATACCCAACGGCTGCACAGTGGCCTTG
GTTACCGGACTCCGCTAGAGTTCGAAGGAATCAACTGATGGGGCGTGTCCATTTTATTGGGGGAAGTCCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 372 bp | 123 aa | 58 | 429 | + | No |
Description : First part of the transposase
ORF sequence :
MPEMITGKKTQQYSTEFKVKAVEWSHQAHRSVKSVAEALDIHPFMLSRWRKEYREGKFAMKRVKKAPADAKKKIQEQDEVARLKRRISELQEENDILKKF
QRFQAEERRKRSGSSGNTDETTV
QRFQAEERRKRSGSSGNTDETTV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 945 bp | 295 aa | 294 | 1238 | + | No |
Description : Second part of the transposase
ORF sequence :
GCPPETPDIGASGGKRHSKKVSTFSGRGTTEAFRFIWKHRRDYSVKALCRHLKVSRSGYYAWANREPSKRATDKADLLLKIRKIYDNSKGRYGSPRVYQT
LRREGLVVGENRVAKIMREWGMKARSARVYRRLRKLRNDLKALPNYRLEAARPTAENQQWSSDVTYIKLGKKYVFLAVVLDLYSRRIISWRLGASLRANF
ARATLREAFTSRKPDSGLLFHTDRGIEYRAHKTQALLKQYQVKHSMNRPGQCADNAEVESFFKTLKGELLHATSFVTMRQLRKHIKAYIEGFYNTQRLHS
GLGYRTPLEFEGIN
LRREGLVVGENRVAKIMREWGMKARSARVYRRLRKLRNDLKALPNYRLEAARPTAENQQWSSDVTYIKLGKKYVFLAVVLDLYSRRIISWRLGASLRANF
ARATLREAFTSRKPDSGLLFHTDRGIEYRAHKTQALLKQYQVKHSMNRPGQCADNAEVESFFKTLKGELLHATSFVTMRQLRKHIKAYIEGFYNTQRLHS
GLGYRTPLEFEGIN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1181 bp | 392 aa | 58 | 1238 | + | Yes |
Chemistry : DDE
ORF sequence :
MPEMITGKKTQQYSTEFKVKAVEWSHQAHRSVKSVAEALDIHPFMLSRWRKEYREGKFAMKRVKKAPADAKKKIQEQDEVARLKRRISELQEENDILKKV
STFSGRGTTEAFRFIWKHRRDYSVKALCRHLKVSRSGYYAWANREPSKRATDKADLLLKIRKIYDNSKGRYGSPRVYQTLRREGLVVGENRVAKIMREWG
MKARSARVYRRLRKLRNDLKALPNYRLEAARPTAENQQWSSDVTYIKLGKKYVFLAVVLDLYSRRIISWRLGASLRANFARATLREAFTSRKPDSGLLFH
TDRGIEYRAHKTQALLKQYQVKHSMNRPGQCADNAEVESFFKTLKGELLHATSFVTMRQLRKHIKAYIEGFYNTQRLHSGLGYRTPLEFEGIN
STFSGRGTTEAFRFIWKHRRDYSVKALCRHLKVSRSGYYAWANREPSKRATDKADLLLKIRKIYDNSKGRYGSPRVYQTLRREGLVVGENRVAKIMREWG
MKARSARVYRRLRKLRNDLKALPNYRLEAARPTAENQQWSSDVTYIKLGKKYVFLAVVLDLYSRRIISWRLGASLRANFARATLREAFTSRKPDSGLLFH
TDRGIEYRAHKTQALLKQYQVKHSMNRPGQCADNAEVESFFKTLKGELLHATSFVTMRQLRKHIKAYIEGFYNTQRLHSGLGYRTPLEFEGIN
Blast result :
Comments
ISHati1 is 68% aa similar to ISVisp1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Sarah Sonbol (2021) Direct submission.
2] Li,J. (2019) Direct GenBank submission.
2] Li,J. (2019) Direct GenBank submission.