ISAba58

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter baumannii
Acinetobacter baumannii
Acinetobacter sp. WCHAc010034
DNA section
IS Length : 1379 bp

Ends


IR Length : 21/27

IRL : GAGCTATAGTCATTTGTGGTGTATGAGGGGAAAAGAGGGTGGCGTATCCT
IRR : GAGTTATGGTCAAATCTGGTGTACGAGCATCAGGCGGTTTTCCTGTCTGA

Insertion site


Left flankDirect repeatRight flankDR Length
TTCATGGGTACTGCCTTTCAAAAAGTAGCCATAACCACTGTA9

DNA sequence

GAGCTATAGTCATTTGTGGTGTATGAGGGGAAAAGAGGGTGGCGTATCCTGTTGATTGTTTCCTCCAAGAAATAATTAACAGTAGAGCGATACGCCATGA
CAAATATTACCCTGAATGTTCTTTCACAACCAGATGAAACTGGCACTGATGTGCTAACTGCTTTATTGCGTAATGGTGCTCGTCAGCTCATTGCGCAAGC
AGTTGAAGTAGAGCTGCAGCAACTGCTGCAAGCTCATGAAGAACTGCGTTTACCCGATGGCCGCAAAGCTGTGGTGCGTAACGGCTATTTGCCTGAGCGC
AGCATCCAAACGGGCATTGGCGATGTTGATATCAAGGTGCCAAAAGTGCGTGATCGTAGCGGCTCTGGTATTCGTTTTACCAGCGCACTGCTACCGCCAT
ACCTCAAGCGTGCCCGCAGCGTTGAAGAGTTATTACCGTGGCTGTATCTCAAAGGTATTTCCACTGGAGACTACCAAGAAGCCCTTGCAGCCTTGCTGGG
TGAGAATGCCAAAGGTCTGTCAGCTAACACCATTTCCAGGCTCAAGGAACGCTGGATTGATGAGCACCGAGAGTGGCGACAGCGCGATTTGAGTGACAAG
CGCTACGCGTACCTTTGGGTTGACGGTATTTACAGCAACGTCCGCCTCGATGACCGTTTGTGCTTGCTGGTGGTGATGGGCGTGACAGAGCATGGTCGCA
AGGAGCTGATTGCCGTTGAGGAAGGCTATCGCGAGTCTGAAGCCAGCTGGCTTGAGCTGCTGAATGGCCTGGTTGCGCGTGGGCTTACGACCTGCCCAAA
GCTGGCAATAGCTGACGGCGCCTTGGGCTTCTGGAAAGCCCTGAGTAAGGTCTATCCGCAGACCAAACAACAACGCTGTTGGGTGCATAAAACGGCCAAT
GTGCTCAACAAACTGCCCAAAGCAGTACAGCCCAAAGTAAAAGAAGCACTGCATGATATCTGGATGGCAGAGACGCGTGAGAAGGCTCATAAAGCCTTTG
ATATAGCGCTGGAAAGGTTTACTGCTAAGTACCCACGCGCAATGGAATGCTTAGCTAAAGACCGTGAGAGCATGTTGGCTTTCTACGATTTTCCAGCAGA
ACATTGGGTGAGTATACGCACCACCAACCCAATTGAATCTGCCTTCGCTACAGTACGTTTAAGAACCAGCAAAACCCGCAATTGCGGTTCAAGAAACACA
ACCCTAGCAATGGTTTATAAATTGCTGCAATCAGCGCAGAAGCGCTGGAACCGCCTGAAAGGATTTCAACTGCTAACCCTCGTGGTCAATAACGTTAAAT
TCCAAGACGGCGAACAAGTAATGGAGCAATCAGACAGGAAAACCGCCTGATGCTCGTACACCAGATTTGACCATAACTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1254 bp417 aa971350+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTNITLNVLSQPDETGTDVLTALLRNGARQLIAQAVEVELQQLLQAHEELRLPDGRKAVVRNGYLPERSIQTGIGDVDIKVPKVRDRSGSGIRFTSALLP
PYLKRARSVEELLPWLYLKGISTGDYQEALAALLGENAKGLSANTISRLKERWIDEHREWRQRDLSDKRYAYLWVDGIYSNVRLDDRLCLLVVMGVTEHG
RKELIAVEEGYRESEASWLELLNGLVARGLTTCPKLAIADGALGFWKALSKVYPQTKQQRCWVHKTANVLNKLPKAVQPKVKEALHDIWMAETREKAHKA
FDIALERFTAKYPRAMECLAKDRESMLAFYDFPAEHWVSIRTTNPIESAFATVRLRTSKTRNCGSRNTTLAMVYKLLQSAQKRWNRLKGFQLLTLVVNNV
KFQDGEQVMEQSDRKTA

 

Blast result :
Comments
ISAba58 is 87% aa similar to ISMaq7.
This IS element was found in 4 related A. baumannii isolated from four separate patients. The IS element was located within the intrinsic blaOXA-51-like gene.
References
1] Paul Higgins (2020) Direct submission.