ISEc86
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP039405 | ND | Escherichia coli | Escherichia coli 377323_2f plasmid unnamed |
DNA section
IS Length : 1420 bp
Ends
IR Length : 19/25
IRL : TGAAGTGCGCCCCAAAGGTTAGACACCAGACCAAAAACTGAGGATGCATG
IRR : TGAACTGCGCCCCAGATCCTGGACAACTTATGCAGCCTGACTCAATCGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTATCCAAC | AA | AATGATGGAC | 2 |
DNA sequence
TGAAGTGCGCCCCAAAGGTTAGACACCAGACCAAAAACTGAGGATGCATGGAAAAGTTTTCATTGGAGTTCAAAAAGGTAGTATCAGAAAAGTACCTGGA
GGGAGAGCTGAGTCTGAAATCTGTAGCAAGGATGTACGGTATCAGTCCCTGCACAGTAAGAAAATGGTCTTATGCCTATCGGGAACATGGGATAAGCATA
CTTACGGGTAAAAAAGGACGCTATTCTGCTGAGTTAAAACTCACAGTAGTAAAAGAAGTCGTGGATGACTGTTTTTCTGTTCGTGAAGTAGCGGTAAAAC
ACGGAATACCAGCCTTTGGTACTGTATGCAACTGGCTTGAAAAATATAGAAAATATGGCGAAGATGCTTTCATTCGAAAAAATAAAAAGAGTATTCCTGT
GCCGGATAAAAGCGCCATTTCAGCACCACCACTACCAACATTAACCAAAGATGAGAGAGAAGAACTTGAACAACTCAGGGTCGAGAATACCTACTTAAAA
AAGCTGAAAGCCTTGGTTCAGCAGAAAACATGCTCAACGTTCAGGAAAAAGTGAGCATTGTGAATGAACTAAGGCAGGAATGGCCATTGTCCCGATTGTT
AATTGTTTCCGGGTTGCCCCGGAGTACATTTTACTATCATGTCAGACGGCTTGCGGCTCCTGACCGGTATCAGTCTGCCAGAGCGCTTCTGCTGAAAATT
TATCACCAGCACAAAGGCCGCTATGGTTACAGACGCATCAGGCTGGCATGTCGTAACGAGGGGGTTTTACTTAATGGTAAAACCGTCAGAAAACTGATGA
AGGAACTGGGTATTAGCAGCCTGATCCGCGTTAAAAAATACCGTTCATATAAAGGAGAACAAGGACGAATTTGCGATAATCTCCTGAAACGCAATTTTGA
TGCAAAACGCCCCAACGAGAAGTGGGTTACAGATGTCACTGAATTTAAAGTGAATGGCAAGAAGCTGTATTTGTCACCCATCATGGATCTTTATAATGGA
GAAATAATTTCCTATAACCTCGCTACTCGCCCTCAACCTTCAATGGTGCAGACTATGCTTACGGATGCGCTGAAACAGCTGTCGAAAGATGAACACCCCA
TACTGCACAGCGATCAAGGTTGGCAATATCAGATGTCCCGGTGGCAACGATGGTTAAAGGATAGCGGTATAGTCCAGAGTATGTCTCGCCGGGGAAATTG
TCTTGATAATGCAGTTATAGAGAGCTTTTTTGGAACATTGAAATCAGAATGTTATTACCTCAATGAATATAAAAATGTGGAGGACTTAAAGCGAGACATC
ATTGATTACATAAACTATTATAATCAATTGAGGATAAAGGAAAAACTTGGCGGTCTTAGCCCGGTACAATATCGATTGAGTCAGGCTGCATAAGTTGTCC
AGGATCTGGGGCGCAGTTCA
GGGAGAGCTGAGTCTGAAATCTGTAGCAAGGATGTACGGTATCAGTCCCTGCACAGTAAGAAAATGGTCTTATGCCTATCGGGAACATGGGATAAGCATA
CTTACGGGTAAAAAAGGACGCTATTCTGCTGAGTTAAAACTCACAGTAGTAAAAGAAGTCGTGGATGACTGTTTTTCTGTTCGTGAAGTAGCGGTAAAAC
ACGGAATACCAGCCTTTGGTACTGTATGCAACTGGCTTGAAAAATATAGAAAATATGGCGAAGATGCTTTCATTCGAAAAAATAAAAAGAGTATTCCTGT
GCCGGATAAAAGCGCCATTTCAGCACCACCACTACCAACATTAACCAAAGATGAGAGAGAAGAACTTGAACAACTCAGGGTCGAGAATACCTACTTAAAA
AAGCTGAAAGCCTTGGTTCAGCAGAAAACATGCTCAACGTTCAGGAAAAAGTGAGCATTGTGAATGAACTAAGGCAGGAATGGCCATTGTCCCGATTGTT
AATTGTTTCCGGGTTGCCCCGGAGTACATTTTACTATCATGTCAGACGGCTTGCGGCTCCTGACCGGTATCAGTCTGCCAGAGCGCTTCTGCTGAAAATT
TATCACCAGCACAAAGGCCGCTATGGTTACAGACGCATCAGGCTGGCATGTCGTAACGAGGGGGTTTTACTTAATGGTAAAACCGTCAGAAAACTGATGA
AGGAACTGGGTATTAGCAGCCTGATCCGCGTTAAAAAATACCGTTCATATAAAGGAGAACAAGGACGAATTTGCGATAATCTCCTGAAACGCAATTTTGA
TGCAAAACGCCCCAACGAGAAGTGGGTTACAGATGTCACTGAATTTAAAGTGAATGGCAAGAAGCTGTATTTGTCACCCATCATGGATCTTTATAATGGA
GAAATAATTTCCTATAACCTCGCTACTCGCCCTCAACCTTCAATGGTGCAGACTATGCTTACGGATGCGCTGAAACAGCTGTCGAAAGATGAACACCCCA
TACTGCACAGCGATCAAGGTTGGCAATATCAGATGTCCCGGTGGCAACGATGGTTAAAGGATAGCGGTATAGTCCAGAGTATGTCTCGCCGGGGAAATTG
TCTTGATAATGCAGTTATAGAGAGCTTTTTTGGAACATTGAAATCAGAATGTTATTACCTCAATGAATATAAAAATGTGGAGGACTTAAAGCGAGACATC
ATTGATTACATAAACTATTATAATCAATTGAGGATAAAGGAAAAACTTGGCGGTCTTAGCCCGGTACAATATCGATTGAGTCAGGCTGCATAAGTTGTCC
AGGATCTGGGGCGCAGTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 507 bp | 168 aa | 48 | 554 | + | No |
Chemistry : DDE
ORF sequence :
MEKFSLEFKKVVSEKYLEGELSLKSVARMYGISPCTVRKWSYAYREHGISILTGKKGRYSAELKLTVVKEVVDDCFSVREVAVKHGIPAFGTVCNWLEKY
RKYGEDAFIRKNKKSIPVPDKSAISAPPLPTLTKDEREELEQLRVENTYLKKLKALVQQKTCSTFRKK
RKYGEDAFIRKNKKSIPVPDKSAISAPPLPTLTKDEREELEQLRVENTYLKKLKALVQQKTCSTFRKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 470 | 1393 | + | No |
Chemistry : DDE
ORF sequence :
TTQGREYLLKKAESLGSAENMLNVQEKVSIVNELRQEWPLSRLLIVSGLPRSTFYYHVRRLAAPDRYQSARALLLKIYHQHKGRYGYRRIRLACRNEGVL
LNGKTVRKLMKELGISSLIRVKKYRSYKGEQGRICDNLLKRNFDAKRPNEKWVTDVTEFKVNGKKLYLSPIMDLYNGEIISYNLATRPQPSMVQTMLTDA
LKQLSKDEHPILHSDQGWQYQMSRWQRWLKDSGIVQSMSRRGNCLDNAVIESFFGTLKSECYYLNEYKNVEDLKRDIIDYINYYNQLRIKEKLGGLSPVQ
YRLSQAA
LNGKTVRKLMKELGISSLIRVKKYRSYKGEQGRICDNLLKRNFDAKRPNEKWVTDVTEFKVNGKKLYLSPIMDLYNGEIISYNLATRPQPSMVQTMLTDA
LKQLSKDEHPILHSDQGWQYQMSRWQRWLKDSGIVQSMSRRGNCLDNAVIESFFGTLKSECYYLNEYKNVEDLKRDIIDYINYYNQLRIKEKLGGLSPVQ
YRLSQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1346 bp | 448 aa | 48 | 1393 | + | Yes |
Chemistry : DDE
ORF sequence :
MEKFSLEFKKVVSEKYLEGELSLKSVARMYGISPCTVRKWSYAYREHGISILTGKKGRYSAELKLTVVKEVVDDCFSVREVAVKHGIPAFGTVCNWLEKY
RKYGEDAFIRKNKKSIPVPDKSAISAPPLPTLTKDEREELEQLRVENTYLKKAESLGSAENMLNVQEKVSIVNELRQEWPLSRLLIVSGLPRSTFYYHVR
RLAAPDRYQSARALLLKIYHQHKGRYGYRRIRLACRNEGVLLNGKTVRKLMKELGISSLIRVKKYRSYKGEQGRICDNLLKRNFDAKRPNEKWVTDVTEF
KVNGKKLYLSPIMDLYNGEIISYNLATRPQPSMVQTMLTDALKQLSKDEHPILHSDQGWQYQMSRWQRWLKDSGIVQSMSRRGNCLDNAVIESFFGTLKS
ECYYLNEYKNVEDLKRDIIDYINYYNQLRIKEKLGGLSPVQYRLSQAA
RKYGEDAFIRKNKKSIPVPDKSAISAPPLPTLTKDEREELEQLRVENTYLKKAESLGSAENMLNVQEKVSIVNELRQEWPLSRLLIVSGLPRSTFYYHVR
RLAAPDRYQSARALLLKIYHQHKGRYGYRRIRLACRNEGVLLNGKTVRKLMKELGISSLIRVKKYRSYKGEQGRICDNLLKRNFDAKRPNEKWVTDVTEF
KVNGKKLYLSPIMDLYNGEIISYNLATRPQPSMVQTMLTDALKQLSKDEHPILHSDQGWQYQMSRWQRWLKDSGIVQSMSRRGNCLDNAVIESFFGTLKS
ECYYLNEYKNVEDLKRDIIDYINYYNQLRIKEKLGGLSPVQYRLSQAA
Blast result :
Comments
ISEc86 is 69% aa (transposase) similar to ISEcB1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Dongsheng Zhou (2020) Direct submission.
2] Greig,D.R., Dallman,T.J., Gally,D.L. and Jenkins,C. (2019) Direct GenBank submission.
2] Greig,D.R., Dallman,T.J., Gally,D.L. and Jenkins,C. (2019) Direct GenBank submission.