ISfinder Mirror Site

ISCco5

Family IS607
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP006710	ND	Campylobacter coli	Campylobacter coli Campylobacter jejuni subsp. jejuni 00-2544 plasmid

DNA section

IS Length : 2051 bp

Ends

IR Length : 0

IRL : CTTATAACTGCTAAGTAAATTATAAAAAAATCTATAAAATTATATAAATA
IRR : GTTCCTACAAACCTATAAAATCCTATAAATAGATACAAATATCTACAAAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGGTACGGGG		GGTATCTTTA	0

DNA sequence

CTTATAACTGCTAAGTAAATTATAAAAAAATCTATAAAATTATATAAATATTTTATAGATTTTACTTGACTTATTTTTTAAAATTTGCTAAAATAGTCCT
ATGAATAAATTATTATCAATCGGTCAAGCTAGTAAAGCTCTTGGTGTTACTATCCAAACTCTTAGAAATTGGGATAAAAAAGGCTTATTAAAACCTGATG
ATATGACTAAGGGTGGAGAGCGTAGGTATAAGCTAGAAACATTAAAAGCTATAAATAAAAACCTAGTTTTTAATAAAGATAGCTTAAAAACTATTGCTTA
TGCTCGTGTTTCTAGCCACGACCAAAAAGATGATTTAATAAGGCAAGTTCAAGTCTTAGAGCTATATTGTTCTAAACAAGGCTTTAATTATGAAGTTATA
CAAGATTTAGGCTCTGGTATGAATTATTATAAAAAAGGCTTAACTAAATTGCTAAATCAAATTCTAGATGGCAAAGTAAAAAGATTAGTTTTAACTCACA
AAGACAGACTTTTACGCTTTGGTGCTGAGCTAGTATTTGCTATATGTGAAGCCAAAGAAGTAGAAGTAATAATAATAAATAAAGGCGAAGAAAGCGTAAA
ATTTGAAGAAGAATTAGCCAAAGATGTGTTGGAAATAATCACAGTATTTTCAGCTAGACTTTATGGCTCACGCTCAAAGAAAAATAAAAAACTTTTAGAT
GAAATGCAAGAAGTGGTGGTAGAAAATGTCAGCAAATAATAATGAGCTTATTAGCATTTCTCATAAGATAGAGTTAAAGCCAAATAATAAAGCTAAAACT
CATTTTAAAAAAGCCTTTGGTTGTGCTAGACTTGCTTATAATTGGGGACTTGCAAAATGGCAAGAATACTACAAGCAAGGCATTAAAGTAACACATTTAG
ATTTAAAAAAGGAATTTAATGCCATTAAAAAAGAACAATTCCCCTTTGTGTATGAAGTAAGTAAATATGCTACGCAACAGCCTTTTTTAAATCTAAATCT
AGCATTTCAAAAATTTTTTAGAGATTTAAAACAAGGTAAAGTAAGCTATCCAAAATTTAAGAAAAAGAAAGATAATTTCGGCTCTTATTACATAGGTGGA
GACCAAGCAATAATCAAAGATGAAAAATATCTAAAAGTGCCAAATTTAGGACTTGTAAAAATGCGTGAGAATCTACGCTTTAATAGTAAAATAAACTCTG
CTACAATCTCTCAAAAAGGTGATAAATACTTCGCTTCTTTTAGTGTAGAGATTCCACAAGACGAGTTTATAAAAACACATAAGATAAAAGAAAATAAAGG
TGGAATCCTAGGCATTGATATGGGATTAAAGAGTTTTGTAAGTTTATCTAATGGTTTAAGTATAAATGCACCTAAGCCACTAAATAAGCTTACAAGAAAG
CTTAAAAGAGTTAGCAGACAACTTAGTAAAAAACAACACCCAAAAGCCAAAGGTGATAAAACCAAAAAGTCTAACAACTACTTAAAGCAGAACTTGAAGC
TTAGAAAGATTCATACAAAAATAGCAAATATGCGCAATGATTTTTTACACAAATTAACAAGCATTTTAGCTAATCATTATAAATATTTTTGTGTGGAATC
CCTTAATGTGCAAGGAATGATGAAAAATCATAATCTTGCTAGAAGCATATCTGATGTAAGTTTCTTTGAATTTAAAAGAATGCTAGCTTATAAAAGTGCA
TATCGTGGCAATGAAGTCATAGAAGCGGATAGATTTTATCCTAGCTCTAAGACTTGTCATTGCTGTGGATACAAAAAAGAATTTCTAACTTTAAGCGAAC
GCATTTTTCAATGCGAAAATTGTGGAATTACAATAGATAGAGACTATAATGCAAGTCTAAATTTAAAACGATATGCAATAAACAATATAGGCTTAGTTCA
AGCCGAATTTACGCCTGAGGACTTGACGGCTCTGCAAAGTGATTTAGCAATAAATCGTATTGCAACTAGCAAGGTTGATACAGGAATACAACAGAAATCT
TGTTTGTAGATATTTGTATCTATTTATAGGATTTTATAGGTTTGTAGGAAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
639 bp	212 aa	101	739	+	No

ORF function : Transposase
Chemistry : Serine

ORF sequence :

MNKLLSIGQASKALGVTIQTLRNWDKKGLLKPDDMTKGGERRYKLETLKAINKNLVFNKDSLKTIAYARVSSHDQKDDLIRQVQVLELYCSKQGFNYEVI
QDLGSGMNYYKKGLTKLLNQILDGKVKRLVLTHKDRLLRFGAELVFAICEAKEVEVIIINKGEESVKFEEELAKDVLEIITVFSARLYGSRSKKNKKLLD
EMQEVVVENVSK

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1284 bp	427 aa	726	2009	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MSANNNELISISHKIELKPNNKAKTHFKKAFGCARLAYNWGLAKWQEYYKQGIKVTHLDLKKEFNAIKKEQFPFVYEVSKYATQQPFLNLNLAFQKFFRD
LKQGKVSYPKFKKKKDNFGSYYIGGDQAIIKDEKYLKVPNLGLVKMRENLRFNSKINSATISQKGDKYFASFSVEIPQDEFIKTHKIKENKGGILGIDMG
LKSFVSLSNGLSINAPKPLNKLTRKLKRVSRQLSKKQHPKAKGDKTKKSNNYLKQNLKLRKIHTKIANMRNDFLHKLTSILANHYKYFCVESLNVQGMMK
NHNLARSISDVSFFEFKRMLAYKSAYRGNEVIEADRFYPSSKTCHCCGYKKEFLTLSERIFQCENCGITIDRDYNASLNLKRYAINNIGLVQAEFTPEDL
TALQSDLAINRIATSKVDTGIQQKSCL

Blast result :

Comments

ISCco5 is 100% (ORFA : the transposase) and 75% (TnpB) aa similar to ISChh1

References

1] Yizhi Tang (2020) Direct submission.
2] Clark,C.G., Chong,P.M., McCorrister,S.J., Simon,P., Walker,M., Lee,D.M., Nguy,K., Cheng,K., Gilmour,M.W. and Westmacott,G.R. (2013) Direct GenBank submission.