ISAba57
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP028560 | ND | Acinetobacter baumannii | Acinetobacter johnsonii Acinetobacter baumannii pNDM1-010045 Acinetobacter junii |
DNA section
IS Length : 1201 bp
Ends
IR Length : 16/20
IRL : GGTAATCCCCCCTAAAAAAAATAGGATCTAAAAGTAGAATTTTCTCTTAA
IRR : GGTAATCCTCCCATAAATAACCGAAGTTAAAAGTAGAGGTTAAGCAGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACTTAGTATG | AGAG | GGCGTGGTCT | 4 |
DNA sequence
GGTAATCCCCCCTAAAAAAAATAGGATCTAAAAGTAGAATTTTCTCTTAAGATACGAGCAGGAGATTCTGCATGAAAACATCTAAATTTACTGACAGTCA
GATCATGTCCATCTTGAAACAGGCAGAATCTGGCACACCTGTAGCGACCCTTTGCCGTGAACACGGCATGAGTAATGCTACCTTTTATAAATGGCGTGCC
AAATATGGTGGTATGGATACATCATTAATGGCTCGACTGAAAGAGCTAGAAGCCGAAAATACCAGACTTAAAAAGATGTATGCGGAAGAGCGATTAAAGG
CCGAAATCATCCAGGAAGCGATGGCAATGAGCAGCACTGCTGCGCAAGGGTAAAGCCATCTTGCCGGCGTAAGATGGCACAACAGGCAGTTGCCCAGCAT
GCCATTAGTATTCGTTTGGCTTGTAAAGCATTTGGCATTAGTGAAACCTGCTACCGCTATCAAGCCAATCTCAGCGATGACAATGCACTCATTGCTGAAC
AGCTCATTGAACTCACTGAGGAAAATTCCGATTGGGGCTTTGGTTTGTGCTTCTCGTATTTACGGCATGTTGAGAGTTGCTGCTGGAATCACAAGCGGGT
TTACCGCATTTACTGTGAGTTGGCACTGAATCTGCGTATTAAACCGAGAAGAAGACTAAAACGGCATGCGCCTGAACCATTGAAAGAACCAATCCGAGAA
AATCAGGTTTGGTCATTAGATTTTATGCATGATCAGCTTTCCGATGGTCGCAAGTTTCGATTATTGAATGTGATTGATGATTACCGCCGCGAAGGCCTAG
CCATTGAAGCAGGGTTCTCATTGCCCACAATCAGGGTTATTCGTACGTTGAATCAATTGCTGGAGTGGAGAGAAAAACCGTTAGTGATCCGATGCGATAA
TGGTCCCGAATTTATCAGTCACGAATTTGTGCGCTGGGCTACTGAGCACGGTATTCGTATTGAATATATTCAACCGGGTAAGCCACAGCAGAATGCGTAT
ATTGAACGCTATAATCGGACCATACGTTATAGTTGGCTGAGCAAGCATTTATTTGATACTTTGGATGAAGTACAAGATTATGCAACAAACTGGTTGTGGC
ATTACAACCATGAAAGACCACATCAAGCTAACAAAGGAAAGCCACCTCTAATGGCTGCTTAACCTCTACTTTTAACTTCGGTTATTTATGGGAGGATTAC
C
GATCATGTCCATCTTGAAACAGGCAGAATCTGGCACACCTGTAGCGACCCTTTGCCGTGAACACGGCATGAGTAATGCTACCTTTTATAAATGGCGTGCC
AAATATGGTGGTATGGATACATCATTAATGGCTCGACTGAAAGAGCTAGAAGCCGAAAATACCAGACTTAAAAAGATGTATGCGGAAGAGCGATTAAAGG
CCGAAATCATCCAGGAAGCGATGGCAATGAGCAGCACTGCTGCGCAAGGGTAAAGCCATCTTGCCGGCGTAAGATGGCACAACAGGCAGTTGCCCAGCAT
GCCATTAGTATTCGTTTGGCTTGTAAAGCATTTGGCATTAGTGAAACCTGCTACCGCTATCAAGCCAATCTCAGCGATGACAATGCACTCATTGCTGAAC
AGCTCATTGAACTCACTGAGGAAAATTCCGATTGGGGCTTTGGTTTGTGCTTCTCGTATTTACGGCATGTTGAGAGTTGCTGCTGGAATCACAAGCGGGT
TTACCGCATTTACTGTGAGTTGGCACTGAATCTGCGTATTAAACCGAGAAGAAGACTAAAACGGCATGCGCCTGAACCATTGAAAGAACCAATCCGAGAA
AATCAGGTTTGGTCATTAGATTTTATGCATGATCAGCTTTCCGATGGTCGCAAGTTTCGATTATTGAATGTGATTGATGATTACCGCCGCGAAGGCCTAG
CCATTGAAGCAGGGTTCTCATTGCCCACAATCAGGGTTATTCGTACGTTGAATCAATTGCTGGAGTGGAGAGAAAAACCGTTAGTGATCCGATGCGATAA
TGGTCCCGAATTTATCAGTCACGAATTTGTGCGCTGGGCTACTGAGCACGGTATTCGTATTGAATATATTCAACCGGGTAAGCCACAGCAGAATGCGTAT
ATTGAACGCTATAATCGGACCATACGTTATAGTTGGCTGAGCAAGCATTTATTTGATACTTTGGATGAAGTACAAGATTATGCAACAAACTGGTTGTGGC
ATTACAACCATGAAAGACCACATCAAGCTAACAAAGGAAAGCCACCTCTAATGGCTGCTTAACCTCTACTTTTAACTTCGGTTATTTATGGGAGGATTAC
C
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 282 bp | 93 aa | 72 | 353 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSKFTDSQIMSILKQAESGTPVATLCREHGMSNATFYKWRAKYGGMDTSLMARLKELEAENTRLKKMYAEERLKAEIIQEAMAMSSTAAQG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 891 bp | 296 aa | 272 | 1162 | + | No |
Description : Second part of the transposase
ORF sequence :
KDVCGRAIKGRNHPGSDGNEQHCCARVKPSCRRKMAQQAVAQHAISIRLACKAFGISETCYRYQANLSDDNALIAEQLIELTEENSDWGFGLCFSYLRHV
ESCCWNHKRVYRIYCELALNLRIKPRRRLKRHAPEPLKEPIRENQVWSLDFMHDQLSDGRKFRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWR
EKPLVIRCDNGPEFISHEFVRWATEHGIRIEYIQPGKPQQNAYIERYNRTIRYSWLSKHLFDTLDEVQDYATNWLWHYNHERPHQANKGKPPLMAA
ESCCWNHKRVYRIYCELALNLRIKPRRRLKRHAPEPLKEPIRENQVWSLDFMHDQLSDGRKFRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWR
EKPLVIRCDNGPEFISHEFVRWATEHGIRIEYIQPGKPQQNAYIERYNRTIRYSWLSKHLFDTLDEVQDYATNWLWHYNHERPHQANKGKPPLMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1091 bp | 364 aa | 72 | 1162 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSKFTDSQIMSILKQAESGTPVATLCREHGMSNATFYKWRAKYGGMDTSLMARLKELEAENTRLKKMYAEERLKAEIIQEAMAMSSTAAQGRVKPSCR
RKMAQQAVAQHAISIRLACKAFGISETCYRYQANLSDDNALIAEQLIELTEENSDWGFGLCFSYLRHVESCCWNHKRVYRIYCELALNLRIKPRRRLKRH
APEPLKEPIRENQVWSLDFMHDQLSDGRKFRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLVIRCDNGPEFISHEFVRWATEHGIRIEY
IQPGKPQQNAYIERYNRTIRYSWLSKHLFDTLDEVQDYATNWLWHYNHERPHQANKGKPPLMAA
RKMAQQAVAQHAISIRLACKAFGISETCYRYQANLSDDNALIAEQLIELTEENSDWGFGLCFSYLRHVESCCWNHKRVYRIYCELALNLRIKPRRRLKRH
APEPLKEPIRENQVWSLDFMHDQLSDGRKFRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLVIRCDNGPEFISHEFVRWATEHGIRIEY
IQPGKPQQNAYIERYNRTIRYSWLSKHLFDTLDEVQDYATNWLWHYNHERPHQANKGKPPLMAA
Blast result :
Comments
ISAba57 is 95% aa (transposase) similar to ISAca1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frmaeshift.
ISAba57 was found in the same position downstream of the repAci10 gene in the Acinetobacter baumannii plasmids pAB2-OXA58-NDM1 and pNDM1-010045 (GenBank accession CP028560), flanked by a 4 bp target site duplication. A copy of ISAba57 identical to the one in pNDM1-010045 was identified in the chromosome of Acinetobacter junii WCHAJ59 (GenBank accession CP028800), flanked by a different 4 bp target site duplication. A copy that differs from that in pNDM1-010045 by three nucleotides was identified in the chromosome of Acinetobacter johnsonii LXL_C1 (GenBank accession CP031011), where it is not flanked by a target site duplication.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frmaeshift.
ISAba57 was found in the same position downstream of the repAci10 gene in the Acinetobacter baumannii plasmids pAB2-OXA58-NDM1 and pNDM1-010045 (GenBank accession CP028560), flanked by a 4 bp target site duplication. A copy of ISAba57 identical to the one in pNDM1-010045 was identified in the chromosome of Acinetobacter junii WCHAJ59 (GenBank accession CP028800), flanked by a different 4 bp target site duplication. A copy that differs from that in pNDM1-010045 by three nucleotides was identified in the chromosome of Acinetobacter johnsonii LXL_C1 (GenBank accession CP031011), where it is not flanked by a target site duplication.
References
1] Robert Moran (2020) Direct submission.
2] Hu,Y., Feng,Y. and Zong,Z. (2018) Direct GenBank submission.
2] Hu,Y., Feng,Y. and Zong,Z. (2018) Direct GenBank submission.