ISAcsp12
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP032279.1 | ND | Acinetobacter sp. | Acinetobacter sp. Acinetobacter sp. WCHAc010034 Acinetobacter indicus B18 plasmid pB18-2, |
DNA section
IS Length : 1274 bp
Ends
IR Length : 29/36
IRL : TGATGTGTGCTTAAAAAACAGGAGGGTTTAACTTGGTAAACTATACATAC
IRR : TGATGTGTGCTGAATTAGCAGGAGACTTTCACTTGGGAGATTCTAGGCAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAATATATCT | GTC | ATGTAAATAA | 3 |
DNA sequence
TGATGTGTGCTTAAAAAACAGGAGGGTTTAACTTGGTAAACTATACATACTCATTAGGAGTTTACCATGAGCAAGAAACAGAAGACTTACACCGCAGAAT
TTAAAGCTGAAGCCATAAAAGCCATTGAAGAGAACCAAGGTAATGTATCGGAATCCGCTAGACAACTTGGCATTTCAATGCAAACCCTTTCGAACTGGAA
TAACAAAGCAAAGGCTGGAACTTTAGCAGGCACAAAACAGTATTCGCCCGATCTAAACGCCTTACTCGAAGAAAATAAGAAGCTTAAACAACAGCTCAAA
ACAGCTGAAATGGAACGTGAATTTTTAAAAAAGGCAGCAGCGTACTTTGCCAAAGAAAGTCAGTGAGGTACGCCTATATGAAATCGCAAAGCCACTTATT
TCCGATTATTTTAATGGCTCGATTACTTCGTGTATCCGTTTCTCGCTTTTATGATTGGCTAAAGCGAGGCATGAGTAAAAGATCAATTCAGCGAAATCAG
CAGACAATTTTGGTCAAAATAGCCCATGAAGAAACCAAACAAAGCTATGGCTATGTTCGATTAACCAAGCATTTACAAGCGCAAGGTATCAAAATAAGTA
CGTATGCTGTGCGCCAAATAAAAAAATCAAATCAGTTGTATTGTAAGCGCCATAAGCGTTTCAAAAGAACCACGAACAGTGATCACAACCGTTCTATTTA
TGACAATTTACTCAAGCAACAGTTTTCAATGACGAAGCCTAATCTTGCGTGGTCGAGTGACATTACCTACATTTGGACGGCTGAGGGCTGGTTGTACTTG
GCAGCTGTCAAAGATCTTTACACGAAACAGGTTGTTGGCTATAGCTTAAATGAGCGCATGACAGCCAAGCTTGTTTGTGATGCATTGAATATGGCTATTC
GCAATCAGAAACCTGCTAAAGATCTCATTGTACATTCAGATAGAGGCAGCCAATATTGCAGTAATGAATATCGAAACTTACTTGAGAAATTTGATTTTCA
GGGTTCAATGAGCAAAAAAGGCGACTGCTTTGATAATGCACCGATAGAGAGTTTCTGGGGCATACTCAAAAATGAACTAGTCCATCATCGCAACTACAAA
ACAAGGGAAGAAGCCAAAGCGGATATTACAAAATATATAGAGTTATTTTATAATCAGCGAAGAATTCAAAAAGGCTTGGATTTTAAGACACCAAATCAAA
TGGCAGAAGACTTTTATCGGTTAGCTGCCTAGAATCTCCCAAGTGAAAGTCTCCTGCTAATTCAGCACACATCA
TTAAAGCTGAAGCCATAAAAGCCATTGAAGAGAACCAAGGTAATGTATCGGAATCCGCTAGACAACTTGGCATTTCAATGCAAACCCTTTCGAACTGGAA
TAACAAAGCAAAGGCTGGAACTTTAGCAGGCACAAAACAGTATTCGCCCGATCTAAACGCCTTACTCGAAGAAAATAAGAAGCTTAAACAACAGCTCAAA
ACAGCTGAAATGGAACGTGAATTTTTAAAAAAGGCAGCAGCGTACTTTGCCAAAGAAAGTCAGTGAGGTACGCCTATATGAAATCGCAAAGCCACTTATT
TCCGATTATTTTAATGGCTCGATTACTTCGTGTATCCGTTTCTCGCTTTTATGATTGGCTAAAGCGAGGCATGAGTAAAAGATCAATTCAGCGAAATCAG
CAGACAATTTTGGTCAAAATAGCCCATGAAGAAACCAAACAAAGCTATGGCTATGTTCGATTAACCAAGCATTTACAAGCGCAAGGTATCAAAATAAGTA
CGTATGCTGTGCGCCAAATAAAAAAATCAAATCAGTTGTATTGTAAGCGCCATAAGCGTTTCAAAAGAACCACGAACAGTGATCACAACCGTTCTATTTA
TGACAATTTACTCAAGCAACAGTTTTCAATGACGAAGCCTAATCTTGCGTGGTCGAGTGACATTACCTACATTTGGACGGCTGAGGGCTGGTTGTACTTG
GCAGCTGTCAAAGATCTTTACACGAAACAGGTTGTTGGCTATAGCTTAAATGAGCGCATGACAGCCAAGCTTGTTTGTGATGCATTGAATATGGCTATTC
GCAATCAGAAACCTGCTAAAGATCTCATTGTACATTCAGATAGAGGCAGCCAATATTGCAGTAATGAATATCGAAACTTACTTGAGAAATTTGATTTTCA
GGGTTCAATGAGCAAAAAAGGCGACTGCTTTGATAATGCACCGATAGAGAGTTTCTGGGGCATACTCAAAAATGAACTAGTCCATCATCGCAACTACAAA
ACAAGGGAAGAAGCCAAAGCGGATATTACAAAATATATAGAGTTATTTTATAATCAGCGAAGAATTCAAAAAGGCTTGGATTTTAAGACACCAAATCAAA
TGGCAGAAGACTTTTATCGGTTAGCTGCCTAGAATCTCCCAAGTGAAAGTCTCCTGCTAATTCAGCACACATCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 67 | 366 | + | No |
Description : First part of the transposase
ORF sequence :
MSKKQKTYTAEFKAEAIKAIEENQGNVSESARQLGISMQTLSNWNNKAKAGTLAGTKQYSPDLNALLEENKKLKQQLKTAEMEREFLKKAAAYFAKESQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 912 bp | 303 aa | 321 | 1232 | + | No |
Description : Second part of the transposase
ORF sequence :
IFKKGSSVLCQRKSVRYAYMKSQSHLFPIILMARLLRVSVSRFYDWLKRGMSKRSIQRNQQTILVKIAHEETKQSYGYVRLTKHLQAQGIKISTYAVRQI
KKSNQLYCKRHKRFKRTTNSDHNRSIYDNLLKQQFSMTKPNLAWSSDITYIWTAEGWLYLAAVKDLYTKQVVGYSLNERMTAKLVCDALNMAIRNQKPAK
DLIVHSDRGSQYCSNEYRNLLEKFDFQGSMSKKGDCFDNAPIESFWGILKNELVHHRNYKTREEAKADITKYIELFYNQRRIQKGLDFKTPNQMAEDFYR
LAA
KKSNQLYCKRHKRFKRTTNSDHNRSIYDNLLKQQFSMTKPNLAWSSDITYIWTAEGWLYLAAVKDLYTKQVVGYSLNERMTAKLVCDALNMAIRNQKPAK
DLIVHSDRGSQYCSNEYRNLLEKFDFQGSMSKKGDCFDNAPIESFWGILKNELVHHRNYKTREEAKADITKYIELFYNQRRIQKGLDFKTPNQMAEDFYR
LAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1166 bp | 388 aa | 67 | 1232 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKKQKTYTAEFKAEAIKAIEENQGNVSESARQLGISMQTLSNWNNKAKAGTLAGTKQYSPDLNALLEENKKLKQQLKTAEMEREFLKKGSSVLCQRKSV
RYAYMKSQSHLFPIILMARLLRVSVSRFYDWLKRGMSKRSIQRNQQTILVKIAHEETKQSYGYVRLTKHLQAQGIKISTYAVRQIKKSNQLYCKRHKRFK
RTTNSDHNRSIYDNLLKQQFSMTKPNLAWSSDITYIWTAEGWLYLAAVKDLYTKQVVGYSLNERMTAKLVCDALNMAIRNQKPAKDLIVHSDRGSQYCSN
EYRNLLEKFDFQGSMSKKGDCFDNAPIESFWGILKNELVHHRNYKTREEAKADITKYIELFYNQRRIQKGLDFKTPNQMAEDFYRLAA
RYAYMKSQSHLFPIILMARLLRVSVSRFYDWLKRGMSKRSIQRNQQTILVKIAHEETKQSYGYVRLTKHLQAQGIKISTYAVRQIKKSNQLYCKRHKRFK
RTTNSDHNRSIYDNLLKQQFSMTKPNLAWSSDITYIWTAEGWLYLAAVKDLYTKQVVGYSLNERMTAKLVCDALNMAIRNQKPAKDLIVHSDRGSQYCSN
EYRNLLEKFDFQGSMSKKGDCFDNAPIESFWGILKNELVHHRNYKTREEAKADITKYIELFYNQRRIQKGLDFKTPNQMAEDFYRLAA
Blast result :
Comments
ISAcsp12 is 94% aa (transposase) similar to ISAba21.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Jing Wang (2020) Direct submission.
2] Hu,Y., Feng,Y. and Zong,Z. (2017) Direct GenBank submission.
2] Hu,Y., Feng,Y. and Zong,Z. (2017) Direct GenBank submission.