ISAcsp12

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP032279.1 ND Acinetobacter sp.
Acinetobacter sp.
Acinetobacter sp. WCHAc010034
Acinetobacter indicus B18 plasmid pB18-2,
DNA section
IS Length : 1274 bp

Ends


IR Length : 29/36

IRL : TGATGTGTGCTTAAAAAACAGGAGGGTTTAACTTGGTAAACTATACATAC
IRR : TGATGTGTGCTGAATTAGCAGGAGACTTTCACTTGGGAGATTCTAGGCAG

Insertion site


Left flankDirect repeatRight flankDR Length
AAATATATCTGTCATGTAAATAA3

DNA sequence

TGATGTGTGCTTAAAAAACAGGAGGGTTTAACTTGGTAAACTATACATACTCATTAGGAGTTTACCATGAGCAAGAAACAGAAGACTTACACCGCAGAAT
TTAAAGCTGAAGCCATAAAAGCCATTGAAGAGAACCAAGGTAATGTATCGGAATCCGCTAGACAACTTGGCATTTCAATGCAAACCCTTTCGAACTGGAA
TAACAAAGCAAAGGCTGGAACTTTAGCAGGCACAAAACAGTATTCGCCCGATCTAAACGCCTTACTCGAAGAAAATAAGAAGCTTAAACAACAGCTCAAA
ACAGCTGAAATGGAACGTGAATTTTTAAAAAAGGCAGCAGCGTACTTTGCCAAAGAAAGTCAGTGAGGTACGCCTATATGAAATCGCAAAGCCACTTATT
TCCGATTATTTTAATGGCTCGATTACTTCGTGTATCCGTTTCTCGCTTTTATGATTGGCTAAAGCGAGGCATGAGTAAAAGATCAATTCAGCGAAATCAG
CAGACAATTTTGGTCAAAATAGCCCATGAAGAAACCAAACAAAGCTATGGCTATGTTCGATTAACCAAGCATTTACAAGCGCAAGGTATCAAAATAAGTA
CGTATGCTGTGCGCCAAATAAAAAAATCAAATCAGTTGTATTGTAAGCGCCATAAGCGTTTCAAAAGAACCACGAACAGTGATCACAACCGTTCTATTTA
TGACAATTTACTCAAGCAACAGTTTTCAATGACGAAGCCTAATCTTGCGTGGTCGAGTGACATTACCTACATTTGGACGGCTGAGGGCTGGTTGTACTTG
GCAGCTGTCAAAGATCTTTACACGAAACAGGTTGTTGGCTATAGCTTAAATGAGCGCATGACAGCCAAGCTTGTTTGTGATGCATTGAATATGGCTATTC
GCAATCAGAAACCTGCTAAAGATCTCATTGTACATTCAGATAGAGGCAGCCAATATTGCAGTAATGAATATCGAAACTTACTTGAGAAATTTGATTTTCA
GGGTTCAATGAGCAAAAAAGGCGACTGCTTTGATAATGCACCGATAGAGAGTTTCTGGGGCATACTCAAAAATGAACTAGTCCATCATCGCAACTACAAA
ACAAGGGAAGAAGCCAAAGCGGATATTACAAAATATATAGAGTTATTTTATAATCAGCGAAGAATTCAAAAAGGCTTGGATTTTAAGACACCAAATCAAA
TGGCAGAAGACTTTTATCGGTTAGCTGCCTAGAATCTCCCAAGTGAAAGTCTCCTGCTAATTCAGCACACATCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
300 bp99 aa67366+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSKKQKTYTAEFKAEAIKAIEENQGNVSESARQLGISMQTLSNWNNKAKAGTLAGTKQYSPDLNALLEENKKLKQQLKTAEMEREFLKKAAAYFAKESQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
912 bp303 aa3211232+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

IFKKGSSVLCQRKSVRYAYMKSQSHLFPIILMARLLRVSVSRFYDWLKRGMSKRSIQRNQQTILVKIAHEETKQSYGYVRLTKHLQAQGIKISTYAVRQI
KKSNQLYCKRHKRFKRTTNSDHNRSIYDNLLKQQFSMTKPNLAWSSDITYIWTAEGWLYLAAVKDLYTKQVVGYSLNERMTAKLVCDALNMAIRNQKPAK
DLIVHSDRGSQYCSNEYRNLLEKFDFQGSMSKKGDCFDNAPIESFWGILKNELVHHRNYKTREEAKADITKYIELFYNQRRIQKGLDFKTPNQMAEDFYR
LAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1166 bp388 aa671232+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSKKQKTYTAEFKAEAIKAIEENQGNVSESARQLGISMQTLSNWNNKAKAGTLAGTKQYSPDLNALLEENKKLKQQLKTAEMEREFLKKGSSVLCQRKSV
RYAYMKSQSHLFPIILMARLLRVSVSRFYDWLKRGMSKRSIQRNQQTILVKIAHEETKQSYGYVRLTKHLQAQGIKISTYAVRQIKKSNQLYCKRHKRFK
RTTNSDHNRSIYDNLLKQQFSMTKPNLAWSSDITYIWTAEGWLYLAAVKDLYTKQVVGYSLNERMTAKLVCDALNMAIRNQKPAKDLIVHSDRGSQYCSN
EYRNLLEKFDFQGSMSKKGDCFDNAPIESFWGILKNELVHHRNYKTREEAKADITKYIELFYNQRRIQKGLDFKTPNQMAEDFYRLAA

 

Blast result :
Comments
ISAcsp12 is 94% aa (transposase) similar to ISAba21.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Jing Wang (2020) Direct submission.
2] Hu,Y., Feng,Y. and Zong,Z. (2017) Direct GenBank submission.