ISPa123
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP041774 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa Pseudomonas aeruginosa 60503 |
DNA section
IS Length : 1203 bp
Ends
IR Length : 33/42
IRL : TGACCTGCCCCCCGTAAATAGTACGGAAACGATGTAGAGTCCATTCCCAA
IRR : TGACCTGCCCCCAGATTTAGTACGGCGCCGACATAGAGTCCGGGGTTAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGAGACATCG | TTACGGCTCA | 0 |
DNA sequence
TGACCTGCCCCCCGTAAATAGTACGGAAACGATGTAGAGTCCATTCCCAAAGGAATGGCAATGAAGAAACGATTCACGGAAGAACAGATCATCGGCGTGC
TCAAGGAGGCCGATGCGGGTGCCAAGCCTGCTGAACTGTGCCGCAAGCACGGGATCTCCGAGGCGACGTACTACAACTGGAAGGCGAAGTTCGGCGGCAT
GACGGTGTCGGACGCTCAGCGCCTGAAGGAGCTGGAGCTGGAGCTGGAGAACAACAAGCTCAAGAAGCTGTTGGCTGAGTCGATGTTGGACAAAGCGGCG
CTTCAGGATCTCTTAGGCCGAAAGTAGTCAGCCCGCAGGCCAGGCGCGAGGCGGTCAGGACGCTAATGACCGAGCGCGGCATGGGTATCACCCGGGCCTG
CGGGCTCGTAGGGATTTCTCGGTCTCTGTTTCACTACGAGAGCCGGCGCACGGGCGATGCGGCGCTGACTGAACGCATGAAAGAGATGGCGGCGGTCAAG
CGGCGCTATGGTTACCGTCGCATCCACGTGCTGTTGCGCCGCGAGGGCTGGCATTCGAATCACAAACGAGTTTGGCGGCTGTACAGCCAAGCTGGGTTGA
GCGTGCGTCAACGCAAGCGCAAGCGCATCGCGGCAACCGAGCGCGTGGTTCGTCCAGCGGCGACCGGGCCGAATCAGAGTTGGTCAATGGACTTTGTCGC
CGACGGTCTGGCCTATGGTCGCCGGTTCCGCTGCTTGAATATTGTCGATGACTACACTCGCGAATGCTTGGCCATCGAGGTTGATACGTCGCTGCCGGGA
CTGCGGGTTACCAACGTGCTTGAACGGTTGGCAGAAATGCGTGGCCTGCCTCGATCCATCACCGTGGACAACGGGCCGGAGTTTGCCGGCAGGGCGTTGG
ATGCCTGGGCCTATCAGGCAGGAGTCACCCTGTCGTTCATTCGGCCAGGCAAGCCCGTGGAGAATGCTTACATCGAAAGTTTCAATGGCAAGTTCCGCGA
CGAATGCCTCAACGAGCATTGGTTCCTGTCGCTTCGCAAGGCCAAAACCTTGATCGAAAACTGGCGGGTCGAGTACAACACCGATCGGCCTCACAGTGCG
CTAGGATATTTAACCCCTACGCAGTTTGCGCAGGCACATCAGGAAAAAATGCTTTTAACCCCGGACTCTATGTCGGCGCCGTACTAAATCTGGGGGCAGG
TCA
TCAAGGAGGCCGATGCGGGTGCCAAGCCTGCTGAACTGTGCCGCAAGCACGGGATCTCCGAGGCGACGTACTACAACTGGAAGGCGAAGTTCGGCGGCAT
GACGGTGTCGGACGCTCAGCGCCTGAAGGAGCTGGAGCTGGAGCTGGAGAACAACAAGCTCAAGAAGCTGTTGGCTGAGTCGATGTTGGACAAAGCGGCG
CTTCAGGATCTCTTAGGCCGAAAGTAGTCAGCCCGCAGGCCAGGCGCGAGGCGGTCAGGACGCTAATGACCGAGCGCGGCATGGGTATCACCCGGGCCTG
CGGGCTCGTAGGGATTTCTCGGTCTCTGTTTCACTACGAGAGCCGGCGCACGGGCGATGCGGCGCTGACTGAACGCATGAAAGAGATGGCGGCGGTCAAG
CGGCGCTATGGTTACCGTCGCATCCACGTGCTGTTGCGCCGCGAGGGCTGGCATTCGAATCACAAACGAGTTTGGCGGCTGTACAGCCAAGCTGGGTTGA
GCGTGCGTCAACGCAAGCGCAAGCGCATCGCGGCAACCGAGCGCGTGGTTCGTCCAGCGGCGACCGGGCCGAATCAGAGTTGGTCAATGGACTTTGTCGC
CGACGGTCTGGCCTATGGTCGCCGGTTCCGCTGCTTGAATATTGTCGATGACTACACTCGCGAATGCTTGGCCATCGAGGTTGATACGTCGCTGCCGGGA
CTGCGGGTTACCAACGTGCTTGAACGGTTGGCAGAAATGCGTGGCCTGCCTCGATCCATCACCGTGGACAACGGGCCGGAGTTTGCCGGCAGGGCGTTGG
ATGCCTGGGCCTATCAGGCAGGAGTCACCCTGTCGTTCATTCGGCCAGGCAAGCCCGTGGAGAATGCTTACATCGAAAGTTTCAATGGCAAGTTCCGCGA
CGAATGCCTCAACGAGCATTGGTTCCTGTCGCTTCGCAAGGCCAAAACCTTGATCGAAAACTGGCGGGTCGAGTACAACACCGATCGGCCTCACAGTGCG
CTAGGATATTTAACCCCTACGCAGTTTGCGCAGGCACATCAGGAAAAAATGCTTTTAACCCCGGACTCTATGTCGGCGCCGTACTAAATCTGGGGGCAGG
TCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 61 | 327 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRFTEEQIIGVLKEADAGAKPAELCRKHGISEATYYNWKAKFGGMTVSDAQRLKELELELENNKLKKLLAESMLDKAALQDLLGRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 909 bp | 302 aa | 279 | 1187 | + | No |
Description : Second part of the transposase
ORF sequence :
VDVGQSGASGSLRPKVVSPQARREAVRTLMTERGMGITRACGLVGISRSLFHYESRRTGDAALTERMKEMAAVKRRYGYRRIHVLLRREGWHSNHKRVWR
LYSQAGLSVRQRKRKRIAATERVVRPAATGPNQSWSMDFVADGLAYGRRFRCLNIVDDYTRECLAIEVDTSLPGLRVTNVLERLAEMRGLPRSITVDNGP
EFAGRALDAWAYQAGVTLSFIRPGKPVENAYIESFNGKFRDECLNEHWFLSLRKAKTLIENWRVEYNTDRPHSALGYLTPTQFAQAHQEKMLLTPDSMSA
PY
LYSQAGLSVRQRKRKRIAATERVVRPAATGPNQSWSMDFVADGLAYGRRFRCLNIVDDYTRECLAIEVDTSLPGLRVTNVLERLAEMRGLPRSITVDNGP
EFAGRALDAWAYQAGVTLSFIRPGKPVENAYIESFNGKFRDECLNEHWFLSLRKAKTLIENWRVEYNTDRPHSALGYLTPTQFAQAHQEKMLLTPDSMSA
PY
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1127 bp | 375 aa | 61 | 1187 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRFTEEQIIGVLKEADAGAKPAELCRKHGISEATYYNWKAKFGGMTVSDAQRLKELELELENNKLKKLLAESMLDKAALQDLLGRKVVSPQARREAVR
TLMTERGMGITRACGLVGISRSLFHYESRRTGDAALTERMKEMAAVKRRYGYRRIHVLLRREGWHSNHKRVWRLYSQAGLSVRQRKRKRIAATERVVRPA
ATGPNQSWSMDFVADGLAYGRRFRCLNIVDDYTRECLAIEVDTSLPGLRVTNVLERLAEMRGLPRSITVDNGPEFAGRALDAWAYQAGVTLSFIRPGKPV
ENAYIESFNGKFRDECLNEHWFLSLRKAKTLIENWRVEYNTDRPHSALGYLTPTQFAQAHQEKMLLTPDSMSAPY
TLMTERGMGITRACGLVGISRSLFHYESRRTGDAALTERMKEMAAVKRRYGYRRIHVLLRREGWHSNHKRVWRLYSQAGLSVRQRKRKRIAATERVVRPA
ATGPNQSWSMDFVADGLAYGRRFRCLNIVDDYTRECLAIEVDTSLPGLRVTNVLERLAEMRGLPRSITVDNGPEFAGRALDAWAYQAGVTLSFIRPGKPV
ENAYIESFNGKFRDECLNEHWFLSLRKAKTLIENWRVEYNTDRPHSALGYLTPTQFAQAHQEKMLLTPDSMSAPY
Blast result :
Comments
ISPa123 is 77% aa (transposase) similar to ISdet2.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Dongsheng Zhou (2020) Direct submission.
2] Jiang,X. and Zhou,D. (2019) Direct GenBank submission.
2] Jiang,X. and Zhou,D. (2019) Direct GenBank submission.